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Carnoy's Solution: Composition and Uses

Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform, and 10% glacial acetic acid. It can also be made with 1 g of ferric chloride dissolved in a mixture of absolute alcohol, chloroform, and glacial acetic acid. Carnoy's solution is used to enhance lymph node detection, fix tissues for immunohistochemistry and DNA analysis, and fix pap smear and mucus samples for staining.

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Matin Ahmad Khan
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1K views1 page

Carnoy's Solution: Composition and Uses

Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform, and 10% glacial acetic acid. It can also be made with 1 g of ferric chloride dissolved in a mixture of absolute alcohol, chloroform, and glacial acetic acid. Carnoy's solution is used to enhance lymph node detection, fix tissues for immunohistochemistry and DNA analysis, and fix pap smear and mucus samples for staining.

Uploaded by

Matin Ahmad Khan
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Carnoy's solution

It is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid Carnoy's Solution is composed of 1 g of ferric chloride (FeCl3) dissolved in 24 mL of absolute alcohol 12 mL of chloroform 4 mL of glacial acetic acid This is the same proportion by volume as the previous formula, with the addition of ferric chloride.

Uses
Some of the uses of Carnoy's solution are: [ Enhancing lymph node detection during dissection of cadavers. Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus. Applied directly following enucleation for the treatment of keratocystic odontogenic tumors In some cases direct application following enucleation for certain kinds of unicystic ameloblastomas. This appears to decrease the likelihood of recurrence over enucleation alone. Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.] As a fixative for pap smear samples. As a fixative agent for both nuclear and mitochondrial DNA in various tissues. As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acidSchiff base.

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