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Abcam Fluorochrome Chart

This document provides a fluorochrome chart that lists fluorescent dyes, their excitation and emission spectra, and the fluorescent channels they correspond to. It describes how to select fluorochromes based on the instrument being used, choosing bright dyes when possible to minimize compensation between channels. Examples are given of dye combinations and the brightness and level of compensation required. The goal is to select dye combinations that provide high signal with minimal spectral overlap between channels.

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6K views1 page

Abcam Fluorochrome Chart

This document provides a fluorochrome chart that lists fluorescent dyes, their excitation and emission spectra, and the fluorescent channels they correspond to. It describes how to select fluorochromes based on the instrument being used, choosing bright dyes when possible to minimize compensation between channels. Examples are given of dye combinations and the brightness and level of compensation required. The goal is to select dye combinations that provide high signal with minimal spectral overlap between channels.

Uploaded by

yylf2016
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
  • Fluorochrome Chart

Fluorochrome chart

FL

450/50

530/30

585/40

630/30

670/40

720/40

780/60

RB

R-PE-Cy7
Cy7
APC-Cy7
AF700
APC-Cy5.5
PerCP-Cy5.5
DRAQ5
PerCP
AF647
R-PE-Cy5.5
R-PE-Cy5

Legend

Cy5

Fluorescent Channel (FL)


Blue Channel
Green Channel
Yellow Channel
Orange Channel
Red Channel
Far Red Channel

APC
7-AAD
TR

Relative Brightness (RB)


High

AF594

Medium

R-PE-TR

Low

PI

Spectra

AF568

Excitation
Emission

R-PE

Lasers

u Violet laser (405 nm)


u Blue laser (488 nm)
u Yellow laser (561 nm)
u Red laser (640 nm)
u Far Red laser (750 nm)

AF555
Cy3
FITC

Abbreviaions

AF488

AF: Alexa Fluor


BV: Brilliant Blue
Cy: Cyanine
PB: Pacific Blue
FITC: Fluorescein isothiocyanate
R-PE: R-Phycoerythrin
PI: Propidium Iodide
TR: Texas Red
7-AAD: 7 AminoacLnomycinD
APC: Allophycocianin
PerCP: Peridinin Chlorophyll

GFP
PB
BV421
AF405
DAPI

350

400

450

500

How to use this chart

Examples

1 Check your instrument:


Type, number of lasers, filters and detectors dictate the fluorochromes that can be used.
Try to choose a fluorochrome for each laser excitation range

Fluorochrome

2 Select bright dyes:


It is possible to rank available dyes according to their brightness on a particular instrument.
Brightest fluorochromes for dim antibodies and vice versa
3 Minimize spillover:
The amount of spectral overlap will determine whether compensation is required.
Sacrifice brightness to avoid spillover
Avoid spillover from bright cell populations into detectors requiring high sensitivity for those populations

Discover more at abcam.com/fluorochrome-chart


Alexa Fluor is a registered trademark of Life Technologies. Alexa Fluor dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

550

Target Expression

600

Lasers

650

Channels

700

750

Brightness

Compensation

800

850

Combination

FITC
APC

High
Low

Blue
Red

Green
Red

Medium
High

Mild

FITC
PE

High
Low

Blue
Yellow

Green
Yellow

Medium
High

Moderate

PerCP
7-AAD

High
Blue
Red
Low

Poor
Severe
Low Blue Red Medium
(not recommended)

Good

Medium

E-583

Wavelength (nm): 300

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