DissolvedOxygenChangeswithvaryingDepth,Temperature,andSalinity
LabPartners
:AlexaRogers(adog),TafariWilliams(twizzle),JordanHill(Jtown)
Date
:82715
Introduction
Primaryproductivityiswhenaproducercreateschemicalenergyfromlightenergythrough
[Link](GPP)isthetotalenergyproducedandusedfor
[Link]
organismthatisinfulllightandgoingthroughphotosynthesisandanorganisminadarksetting
inwhichitundergoesmorecellularrespiration,whichisthebreakingdownofthischemical
energyandusingittomakeenergyincellularrespiration..Wemeasureprimaryproductivityby
[Link]
[Link]
(NPP)[Link]
energythatisleftoverforaconsumeraftertheproducerhasusedsomeforrespiration.
[Link]
beaffectedbytemperatureorthesalinityofasample.
Thepurposeofthislabistomeasureprimaryproductivitybasedondifferingabioticfactorssuch
astemperature,salinity,andvaryingdepthofawatersample.
Question
:Howdodifferentabioticfactorsimpactdissolvedoxygenlevelsinvariouswater
samples?
Hypothesis
:
[Link],thedissolvedoxygenlevelswillincrease.
[Link],thedissolvedoxygenwilldecrease.
[Link],dissolvedoxygenlevelsincrease.
Variables
:
[Link]
Dependentvariabledissolvedoxygenlevel
Control20degrees(roomtemp)watersample
Constantslight,salinity,watersource
[Link]
Dependentvariabledissolvedoxygenlevel
Controlfreshwater
Constantslight,temp
[Link]
Dependentvariabledissolvedoxygenlevel
Controlinitialwaterdissolvedoxygenlevel
Constantstemp,salinity
MaterialsandProcedure
:
Seelabhandout.
Safety
Seelabhandout.
DataTables:
Table1:Temperature/DOTable
Temperature(degrees
Celsius)
ClassMeanDO(partsper
million)
ClassMean%DO
Saturation(from
nomogram)
20degrees
6.4ppm
65.5
14degrees
7.1ppm
69
Salinity(partsper
thousand)
ClassMeanDO(partsper
million)
ClassMean%DO
Saturation
0ppt
6.45ppm
70
32ppt
4.6ppm
61
LabGroupData
ClassMeanData
InitialDO
5.8
6.1
DarkBottleDO
3.7
3.8375
RespirationRate
(InitialDark)
2.1
2.2625
Table2:Salinity/DOTable
Table3:Respiration
Table4:LabGroupData
Watersource:MeridianParkPond
%Light
DO(ppm)
Gross
NetProductivity
Productivity(Light (Light
BottleDarkLight) BottleInitial)
100
5.55
1.85
.25
65
3.2
.5
2.6
25
3.8
.1
2.0
10
3.3
.4
2.5
4.2
.5
1.6
Table5:ClassMeanData
%Light
GrossProductivityDO
(ppm)
NetProductivityDO
(ppm)
100%
2.3
.03
65%
.9
1.4
25%
.7
1.6
10%
.5
1.7
2%
.8
1.5
Graphs
PartA:
PartC:
Conclusion
Discussion
[Link]
utilization,therateofformationoforganiccompounds(glucose),ortherateofoxygen
production.
2.TofindtheamountofcarbonfixedinthesampleyoumultiplyyourO2ppmby0.698toget
themLofO2perliterofsolution.Thenyoutakethatanswerandmultiplyitby0.536tocalculate
[Link],wefixed0.011mgof
Carbon.
[Link],theamountofoxygenthatthewaterholdsincreases.
[Link],[Link]
becausethesalttakesupspaceinsidethesolutionandthereforeleaveslessspaceforoxygen
todissolve.
5.A)Grossproductivitywillbe0inthedarkbottle(orat0%light).
B)Netproductivitywillbe0atabout99.9%light.
[Link]
freestateinwaterissignificantlylessthanthatinair.
[Link],becausestreams
aremoreshallowandphotosynthesisoccursthroughoutmoreofthebodyofastreamthana
[Link]
subsequentlyincreasingtheamountofdissolvedoxygeninthestreamaswell.
8.TheDOconcentrationwouldbegreaterat5pmthanat7amsinceithashadmoresunlight
throughoutthedaytodophotosynthesisandat7am,respirationhasbeenhappeningallnight
andoxygenlevelsarelower.
[Link],typically
compoundscontainingnitrogen,phosphorus,[Link]
[Link]
overcrowdingandcausesspeciestocompeteforresourcesandspace.
[Link],glassAwouldhavemoreoxygenavailablebecauseitismorewatersoahigher
[Link],Bwillhavealargeropportunity
togaindissolvedoxygenbecausethereisalargersurfaceareaofwaterincontactwiththeair.
[Link],theeffectsof
temperaturechangesondissolvedoxygenlevels,ourhypothesiswasthatasthetemperatureof
thefreshwaterdecreases,thedissolvedoxygen(DO)[Link]
increasedinthesampleatalowertemperaturebecausetheoxygenmoleculesaremore
[Link],thesalinityinpartperthousand(ppt)wastestedto
[Link],
[Link],thehigheronehadalower
[Link],forpartC,[Link]
aslightintensitydecreased,[Link]
sample,[Link],lightintensity
wastestedover24hours,[Link]
letintothesample,[Link],theless
photosynthesiscanoccur,sowhentherespirationoccursitlowerstheconcentrationofoxygen
[Link],thelesslightwasreachingthewater,soless
photosynthesiswashappeningandthenetprimaryproductivity(NPP)wasalowerandlower
number,[Link],we
calculatedtheNPPofoursamplesanditdecreasedasthedepthincreased(orthelight
intensitydecreased).So,whenitisnegative(asmanyofourswere),thereismorerespiration
thanphotosynthesishappening,sonoconsumerscangainanyenergy.
[Link]
incorrectasthewrongnumberofscreenswasplacedontwoofthebottlesinitially,however
onlyarelativelysmallamountoftimepassedinwhichthelightpercentagewasincorrect.
AnothermistakethatmayhavebeenmadebyourgroupwasduringthetitrationsoftheDO
[Link]
[Link]
lettingitsettle,howeverforthe25%lightexposurebottle,theprecipitatedissolvedalmost
[Link]
[Link]
isifitispossibletodeterminethepopulationdensityofcertainphotosyntheticorganismswithin
asampleoflakewaterbasedupontheamountofdissolvedoxygeninasample.
