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Technical Data
TB Broth Base w/o Tween 80 M034
Intended Use:
Recommended as a liquid medium for cultivation of Mycobacteria from clinical samples when presence of oleic acid ester is
undesirable. It can also be used for determining antibiotic sensitivity.
Composition**
Ingredients g/L
Proteose peptone 4.000
Yeast extract 2.000
Disodium hydrogen phosphate 2.500
Potassium dihydrogen phosphate 1.000
Sodium citrate 1.500
Magnesium sulphate 0.600
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 11.6 grams in 1000 ml purified / distilled water, which if desired contains 5 ml glycerol (tested to be
non-inhibitory to typical cultures). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°
C and enrich with dextrose to a final concentration of 0.5% and either bovine albumin fraction-V or serum as desired.
Principle And Interpretation
TB Broth media are based on the medium formulated by Dubos and Davis (1) and are used as liquid media for the
cultivation of Mycobacterium tuberculosis. This medium provides dispersed growth of tubercle bacilli which is free of
excessive clumps and so it can be used to prepare a uniform suspension of Mycobacteria. The medium can be used without
additives and supplements; however sterile dextrose and sterile serum can be added for enrichment. Glycerol addition helps
in the cultivation of Mycobacterium tuberculosis though some bovine strains are inhibited by it.
Proteose peptone and yeast extract provide nitrogenous nutrients like amino acids and peptides, vitamin B complex and other
essential nutrients. The medium is well buffered by phosphates. The salts present in the medium supply ions required for the
mycobacterial metabolism. Sodium citrate inhibits gram-positive organisms and coliforms.
Type of specimen
Clinical samples : Sputum
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/
protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and
culture. Standard precautions as per established guidelines should be followed while handling clinical specimens.
Safety guidelines may be referred in individual safety data sheets.
Limitations
1. Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow coloured clear solution without any precipitate.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Reaction
Reaction of 1.16% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 2-4 weeks.
Organism Growth
Mycobacterium kansasii luxuriant
ATCC 12478
Mycobacterium smegmatis luxuriant
ATCC 14468
M. tuberculosis H37 RV luxuriant
25618
Storage and Shelf Life
Store below 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product
performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with
clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
Reference
1. Dubos and Davis, 1946, J. Exp. Med., 83:409.
2. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
3. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Revision :05/2024
HiMedia Laboratories Pvt. Limited, IVD In vitro diagnostic 30°C Storage temperature
Plot No.C-40, Road No.21Y, medical device
MIDC, Wagle Industrial Area,
Thane (W) -400604, MS, India 10°C
EC REP CEpartner4U, Esdoornlaan 13, Do not use if
CE Marking
3951DB Maarn, NL package is damaged
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Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and
other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best
of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related
to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further
manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or
implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Corporate Office : Plot No.C-40, Road No.21Y, MIDC, Wagle Industrial Area, Thane (W) - 400604, India.
Customer care No.: 022-6147 1919 Email: techhelp@[Link] Website: [Link]
