APLab#12:DissolvedOxygenandPrimaryProductivity
PaulaZdanowski,IvyZhou,KylieOHara,SaraKuramitsu
April13,2015
Gold1
[Link]
Abstract
Theobjectiveofthislabwastocalculatedissolvedoxygenconcentrationanddetermine
[Link],thedissolvedoxygenin
[Link],tomeasurethe
productivityofanaquaticenvironment,differentbottleswithvaryingdegreesoflightwereleft
underalightfor24hours,andtitratedagaintodeterminethedissolvedoxygenconcentration.
Theresultsofthislabdemonstratednostrongcorrelationbetweentheintensityofthelightand
thenetproductivity,sosomethingotherthanlightmusthavebeeninfluencingtheproductivityin
theaquaticenvironment.
Introduction
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neededinvirtuallyallmetabolicprocesses,dissolvedoxygenisagreatindicatorofwaterquality
(Robson).Becausewaterisnotasefficientatholdingoxygenasairis,itisimperativethat
[Link]
[Link]
dissolvedoxygencontentofabodyofwater,includingtemperature,wind,turbulence,andthe
trophicstate(HargreavesandTucker).Ameasureoftheproductionofoxygenoveraperiodof
timeisfoundbycalculatingtheamountofcarbonthathasbeenfoundonorganiccompounds
overtime(Sanchez).Theobjectivesofthisexperimentweretodemonstrateimportanceof
carbonandoxygencyclesinanecosystem,theeffectoflightandnutrientsonphotosynthesis,
andtoidentifythefactorsthataffectthesolubilityofdissolvedgassesinaquaticenvironments..
Wehypothesizedthatthetemperatureofthewaterwouldimpacttheamountofdissolved
oxygen,andthatthebottleswithlowerlightwouldbelessproductive.
Procedure/Materials/Methods
Theobjectiveofthislabistomeasurethedissolvedoxygenconcentration,calculate
productivityandrespirationrate,anddemonstratethisknowledgeincorrespondencewiththe
[Link],[Link],
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bottleinawhiteshallowpanandaddedeightdropsofmanganoussulfateandeightdropsof
[Link],aprecipitateformed
[Link]
settleandthenaddedonescoopofacidintothebottleandalsomixeditbyinvertingthebottle.
Whenweaddedtheacid,theprecipitatedissolvedandthesolutionturnadarkyellow.
[Link]
[Link],weaddedeightdropsofstarchindicator
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sodiumthiosulfateandwasusedtotitratethesampleuntilthecolorchangedfrompurpletopale
yellow/[Link]
[Link].
[Link]
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[Link],wewrappedthebottleswiththeappropriateamount
ofscreenforthelightintensities.Thiscanbeseeninthetableonpage11.(Bottle2hadfoil
aroundit,bottle3had1screen,etc...)Weliedbottles27downontheirsidesunderalight
[Link],wepreparedawetmountofthewatersampleinbottle1andobserveditundera
[Link].Wealsopreparedbottle1the
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wepreparedtheotherbottlesaccordingtothesamestepsasbeforeinthepreviouslabandbottle
#[Link],[Link]
[Link]
[Link],wegraphedthedatagiventomeasurethe
productivityofabodyofwater.
Data
Table1:DissolvedOxygenConcentration,LabGroupSample
Temperature
DissolvedOxygen(mg/L)
%Saturation
20C
1mg/L
10%
Table2:DissolvedOxygenConcentration,ClassSample
Temperature(Celsius)
DissolvedOxygen(mg/L)
%Saturation
0(Group1)
0.7
5%
0(Group6)
0.9
8%
20(Group2)
0.97
10%
20(Group5)
0.88
10%
30(Group3)
0.96
12%
30(Group4)
0.75
11%
Astemperatureincreases,thedissolvedoxygenandthe%saturationalsogenerallyincreases.
Table3:GrossandNetProductivity/RespirationRate,ClassSample
PercentLight
Dissolved
Oxygen(mg/L)
NetProductivity Gross
Productivity
(mlO2/L)
Gross
Productivity
(mgC/m^3)
Initial
0.83
Dark
0.80
100%
0.79
0.04
0.01
5.36
65%
0.83
0.03
16.08
25%
0.78
0.05
0.02
10.72
10%
0.91
0.08
0.11
58.96
2%
0.69
0.14
0.11
58.96
Respiration=InitialBottleDarkBottle
NetPrimaryProduction=LightBottleInitialBottle
GrossProduction=LightBottleDarkBottle
Thereisnotastrongcorrelationbetweenthepercentlightexposedandthedissolvedoxygen.
[Link]
specificcorrelationbetweenlightpercentageandnetproductivityaswellaslightpercentageand
grossproductivity.
Discussion/Conclusion
ForPartA,ourfindingsshowthatthetemperatureofthewaterdidimpactthedissolved
[Link],thehigherthedissolved
[Link]
[Link],theyareable
toslowdowntheirmetabolisms,whilestillmaintainingahighenoughoxygenintaketosurvive.
ForpartB,ourfindingsshowthatlighthaslittleeffectontheproductivityofaquatic
environments,[Link]
penetratewaterverywell,whichmeansthatorganismslivingdeepinwaterarelimitednotby
light,[Link]
theamountoflightdidnotreallylimittheproductivity.
WorksCited
Hargreaves,JohnA.,[Link]."MeasuringDissolvedOxygenConcentrationin
Aquaculture."
TheFishSite
.5mPublishing,[Link].20Apr.2015.
Robson,MarkG."DissolvedOxygen."
[Link]
.HighBeamResearch,[Link].20
Apr.2015.
Sanchez,Enrique."UseoftheWaterQualityIndexandDissolvedOxygenDeficitasSimpleIndicators
ofWatershedsPollution."
UseoftheWaterQualityIndexandDissolvedOxygenDeficitas
SimpleIndicatorsofWatershedsPollution
.ElsevierLtd.,[Link].20Apr.2015.
Questions
1) Howdoestemperatureaffectthesolubilityofoxygeninwater?
Thelowerthetemperature,themoresolubleoxygenisinwater.
2)Howdoessalinityaffectthesolubilityofoxygeninwater?
Thehigherthesalinity,thelowerdissolvedoxygenthereisinwater.
3)Wouldyouexpecttofindhigherdissolvedoxygencontentinabodyofwaterinwinteror
summer?
Iwouldexpecttheretobeahigherdissolvedoxygencontentinabodyofwaterinthe
winterbecausethewateriscolder.
4)Discusshoweachofthefollowingfactorscouldinfluencethedissolvedoxygen
concentrationinabodyofwater:
Wind:Windwouldincreasethedissolvedoxygenconcentrationbecauseitmovesair
acrossthesurfaceofthewaterallowingmoreoxygentobeintroduced.
Temperature:Thehigherthetemperature,thelowertheamountofdissolvedoxygenin
water.
Altitude:Asaltitudeincreases,thedissolvedoxygencontentwoulddecreasebecauseof
thedropinpressure.
5)Doyouthinkitwouldbewisetostockapondwithgamefishifithadadissolvedoxygen
contentof3ppm?Whyorwhynot?
Itwouldnotbewisebecausemostfishneedadissolvedoxygencontentofatleast5or6
ppm.
6)Ichosetolabelthelowdissolvedoxygencontentassummer,andthehighdissolved
oxygencontentaswinterbecausetemperatureisindirectlyrelatedtothelevelof
dissolvedoxygeninwater.
7)InpartB,wereanyofthesampleslightlimited?Why?
Itdoesntappearthatanyofthesampleswerelightlimited,becausetherewasnotrendin
[Link]
environmentsaremorelimitedbytheirnutrientssincelightcannotpenetratedeeplyinto
water.
8)BasedonyouranalysisofthelakespresentedinpartCofthelab,whichlakeismore
productive?
Lake2appearstobemoreproductivebecauselightcanpenetratedeeperintothewater
thaninlake1.
9)Whatisusedasthebasisformeasuringprimaryproductivity?
Dissolvedoxygencontentisusedasthebasisformeasuringprimaryproductivitybecause
itcandemonstratetheamountofO2usedinrespiration.
10)
11)[Link]:producesmanganoushydroxidewhenaddedwithwater
[Link]:oxidizesmanganoushydroxidetomanganichydroxide
[Link]:convertsmanganichydroxidetomanganicsulfate
[Link]:makestitrationendpointmorevisible
[Link]:freeiodinecombinesintosodiumiodide
