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Qualitative Tests for Amino Acids

This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

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Thonieroce Apryle Jey Morelos
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625 views2 pages

Qualitative Tests for Amino Acids

This document describes several qualitative tests used to detect different amino acids and peptide bonds in proteins. It lists the name of each test, what biochemical it detects, the reagents used, and the expected color change result. Some of the major tests mentioned are the Biuret test for peptide bonds, Ninhydrin test for alpha-amino acids, Xanthoproteic test for aromatic amino acids, and Millon's test for amino acids containing phenol groups like tyrosine.

Uploaded by

Thonieroce Apryle Jey Morelos
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

QUALITATIVE TEST TEST FOR REAGENT RESULT/S

- Biuret Reagent:
General test for the Hydrated CuSO4
BIURET TEST presence of peptide Potassium Hydroxide Blue-Violet Color
bonds Solution
Potassium Sodium
Tartrate

NINHYDRIN TEST For alpha-amino acids - Ninhydrin Solution Blue Violet Color

To detects the side


chains of aromatic
XANTHOPROTEIC TEST amino acids or to - Conc. HNO3 Clear Yellow
differentiate aromatic - Conc. NaOH Color
amino acids from
other amino acids

For detecting amino


acid containing - Millon’s Reagent Brick Red Color
MILLON’S TEST phenol group
(tyrosine)

For detecting amino - Hopkins-Cole Purple Ring


HOPKINS-COLE TEST acid tryptophan Reagent Coloration at the
present in protein - Conc. H2SO4 interface

For detecting - 10% NaOH


presence of - 0.02% Naphthol Red Color
SAKAGUCHI TEST guanidium group Solution
(arginine) - 2% NaOBr

For determining the


presence of cysteine, - 3M NaOH
NITROPRUSSIDE TEST the only amino acid - 2% Nitroprusside Red Color
containing a sulfhydryl Solution
group (-SH)
For detecting the - 30% NaOH
FOHL’S TEST presence of sulfur - 5% Pb(CH3COO)2 Appearance of
containing amino Black sediment
acids

For detecting Red litmus paper


TEST FOR AMIDES asparagine and - 20% NaOH should turn to blue
glutamine

For detecting - Diazo Reagent:


PAULY’S TEST aromatic amino acid 1% Sulfanilic Acid & Red Color
tyrosine and histidine 5% NaNO2 Solution
- 10% Na2CO3

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