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Cold Drink Composition Analysis

This project investigates the contents of various cold drinks. Tests were conducted to detect the presence of pH, carbon dioxide, glucose, phosphate, and alcohol. The pH of different drinks ranged from 2.5-4. Carbon dioxide was detected when lime water turned milky. Glucose was identified using Benedict's reagent and Fehling's solution, producing reddish precipitates. Phosphate presence was shown by a canary-yellow precipitate with ammonium molybdate and nitric acid. Alcohol presence was indicated by a yellow precipitate using iodine, potassium iodide and sodium hydroxide. The results showed that all drinks contained the tested components.

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khushi kasana
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Topics covered

  • pH detection,
  • carbon dioxide test,
  • carbohydrates,
  • health effects,
  • multinational brands,
  • market analysis,
  • organic compounds,
  • bibliography,
  • safety precautions,
  • teacher guidance
0% found this document useful (0 votes)
111 views18 pages

Cold Drink Composition Analysis

This project investigates the contents of various cold drinks. Tests were conducted to detect the presence of pH, carbon dioxide, glucose, phosphate, and alcohol. The pH of different drinks ranged from 2.5-4. Carbon dioxide was detected when lime water turned milky. Glucose was identified using Benedict's reagent and Fehling's solution, producing reddish precipitates. Phosphate presence was shown by a canary-yellow precipitate with ammonium molybdate and nitric acid. Alcohol presence was indicated by a yellow precipitate using iodine, potassium iodide and sodium hydroxide. The results showed that all drinks contained the tested components.

Uploaded by

khushi kasana
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Topics covered

  • pH detection,
  • carbon dioxide test,
  • carbohydrates,
  • health effects,
  • multinational brands,
  • market analysis,
  • organic compounds,
  • bibliography,
  • safety precautions,
  • teacher guidance

GREATER VALLEY SCHOOL, GREATER NOIDA

HS, P-7, SECTOR OMEGA-2, GREATER NOIDA

[Link] BUDH NAGAR

AN INVESTTIGATORY PROJECT
ON
DETERMINATION OF THE CONTENTS OF COLD DRINKS

SUBMITTED BY
NAME ROLL NO.

UNDER THE GUIDANCE OF


MS Upasana Sharma
Acknowledgement

I would like to express my gratitude to my teacher (Ms


Upasana Sharma) as well as our (Principal Anamika
Sood) who gave me the golden opportunity to do this
wonderful project on the topic (Determination of the
contents of cold drinks) which also helped me in doing
a lot of research and I came to know about so many
new things. I am really thankful to them. Secondly I
would also like to thank my parents and friends who
helped me a lot in finalizing my project.
GREATER VALLEY SCHOOL, GREATER NOIDA
CERTIFICATE

This is to certify that work done by Khushi


Kasana of class XII (Session 2019-2020) in partial
fulfilment of CBSE exam 2020 has been carried
out under my direct supervision and guidance.

Signature of Student Signature of Teacher


Contents
1. Introduction
2. Theory
3. Aim
4. Apparatus
5. Chemicals Required
6. Detection of pH
7. Test for Carbon Dioxide
8. Test for Glucose
9. Test for Phosphate
10. Test for Alcohol
11. Test for Sucrose
12. Result
13. Precautions
14. Conclusion
15. Bibliography
Introduction
The era of cold drinks began in 1952 but the
industrialization in India marked its beginning
with launching of Limca and Gold spot by
parley group of companies. Since, the
beginning of cold drinks was highly profitable
and luring, many multinational companies
launched their brands in India like Pepsi and
Coke.
Now days, it is observed in general that
majority of people viewed Sprite, Fanta and
Limca to give feeling of lightness, while Pepsi
and Thumps Up to activate pulse and brain.
Theory
Cold drinks of different brands are composed of alcohol,
carbohydrates, carbon dioxide, phosphate ions etc. These soft drinks
give feeling of warmth, lightness and have a tangy taste which is
liked by everyone. Carbon dioxide is responsible for the formation of
froth on shaking the bottle.

The carbon dioxide gas is dissolved in water to form carbonic acid


which is also responsible for the tangy taste. Carbohydrates are the
naturally occurring organic compounds and are major source of
energy to our body. General formula of carbohydrates is CX (H2O)Y.

On the basis of their molecule size carbohydrates are classified as:

Monosaccharide, Disaccharides and Polysaccharides.

Glucose is a monosaccharide with formula C6H12O6 .It occurs in Free


State in the ripen grapes in bones and also in many sweet fruits. It is
also present in human blood to the extent of about 0.1%. Sucrose is
one of the most useful disaccharides in our daily life. It is widely
distributed in nature in juices, seeds and also in flowers of many
plants. The main source of sucrose is sugar cane juice which contain
15-20 % sucrose and sugar beet which has about 10-17 % sucrose.
The molecular formula of sucrose isC 12 H 22 O11. It is produced by a
mixture of glucose and fructose. It is non-reducing in nature whereas
glucose is reducing. Cold drinks are a bit acidic in nature and their
acidity can be measured by finding their pH value. The pH values also
depend upon the acidic contents such as citric acid and phosphoric
acid.
APPARATUS
 Test Tube
 Test Tube Holder
 Test Tube Stand
 Stop Watch
 Beaker
 Burner
 pH Paper
 Tripod Stand
 China Dish
 Wire Gauge
 Water Bath
CHEMICALS REQUIRED
 Iodine Solution
 Potassium Iodine
 Sodium Hydroxide
 Fehling’s A & B Solution
 Lime Water
 Concentrated HNO 3

 Benedict Solution
 Ammonium Molybdate
DETECTION OF PH
EXPERIMENT
Small samples of cold drinks of different brands were taken in a test
tube and put on the pH paper. The change in the colour of pH paper
was noticed and was compared with the standard pH scale.

OBSERVATION
[Link] Name of the Colour change pH value
drink
1. COCA COLA PINK 2.5-3
2. SPRITE RED 3
3. LIMCA PINKISH 4
4. FANTA LIGHT ORANGE 3-4

INFERENCE
Soft drinks are generally acidic because of the presence of citric acid
and phosphoric acid. pH values of cold drink of different brands are
different due to the variation in amount of acidic contents.
TEST FOR CARBON DIOXIDE
EXPERIMENT
As soon as the bottles were opened, one by one the sample was
passed through lime water. The lime water turned milky.

OBSERVATON

[Link]. NAME OF THE TIME TAKEN CONCLUSION


DRINK (SEC)
1. COCA COLA 26.5 CO 2IS PRESENT

2. SPRITE 21 CO 2IS PRESENT


3. LIMCA 35 CO 2IS PRESENT
4. FANTA 36 CO 2IS PRESENT

INFERENCE
All the soft drinks contain dissolved carbon dioxide in water. The
carbon dioxide (CO 2) dissolves in water to form carbonic acid, which
is responsible for its tangy taste.

CHEMICAL REACTION INVOLVED


Ca(OH )2(s) + CO 2(g) CaCO3(s) + H 2O(s)
TEST FOR GLUCOSE
EXPERIMENT
Glucose is a reducing sugar acid. Its presence is detected by the
following test:

1. BENEDICTS’S REAGENT TEST:


Small samples of cold drinks of different brands were taken in a test
tube and a few drops of Benedict’s reagent were added. The test
tube was heated for few seconds. Formation of reddish colour
confirmed the presence of glucose in cold drinks.
OBSERVATON

[Link] NAME OF THE OBSERVATION CONCLUSION


DRINK
1. COCA COLA REDDISH GLUCOSE IS PRESENT
COLOUR
PRECIPITATE
2. SPRITE REDDISH GLUCOSE IS PRESENT
COLOUR
PRECIPITATE
3. LIMCA REDDISH GLUCOSE IS PRESENT
COLOUR
PRECIPITATE
4. FANTA REDDISH GLUCOSE IS PRESENT
COLOUR
PRECIPITATE

INFERENCE
All the samples gave positive test for glucose with Benedict’s reagent. Hence
all the drinks contain glucose
2. FEHLING’S SOLUTION TEST
Small samples of cold drinks of different brands were taken in a test
tube and a few drops of Fehling’s A solution and Fehling’s B solution
was added in equal amount. The test tube was heated in a water
bath for 10 minutes. Appearance of brown precipitate confirmed the
presence of glucose in cold drinks.

OBSERVATON
[Link] NAME OF THE OBSERVATION CONCLUSION
DRINK
1. COCA COLA REDDISH BROWN GLUCOSE IS
PRECIPITATE PRESENT
2. SPRITE REDDISH BROWN GLUCOSE IS
PRECIPITATE PRESENT
3. LIMCA REDDISH BROWN GLUCOSE IS
PRECIPITATE PRESENT
4 FANTA REDDISH BROWN GLUCOSE IS
PRECIPITATE PRESENT

INFERENCE
All the samples gave positive test for glucose with Fehling’s (A & B)
solutions. Hence all the cold drinks contain glucose.
TEST FOR PHOSPHATE
EXPERIMENT
Small samples of each brand of cold drinks were taken in separate
test tubes and Ammonium Molybdate followed by concentrated
Nitric Acid (HNO3) was added to it. The solution was heated.
Appearance of canary-yellow precipitate confirmed the presence of
phosphate ions in cold drinks.

OBSERVATON
[Link] NAME OF THE OBSERVATION CONCLUSION
DRINK
1. COCA COLA CANARY-YELLOW PHOSPHATE IS
PRECIPITATE PRESENT
2. SPRITE CANARY-YELLOW PHOSPHATE IS
PRECIPITATE PRESENT
3. LIMCA CANARY-YELLOW PHOSPHATE IS
PRECIPITATE PRESENT
4. FANTA CANARY-YELLOW PHOSPHATE IS
PRECIPITATE PRESENT

INFERENCE
All the soft drinks samples gave positive test for phosphate ions.
Hence all the cold drinks contain phosphate.

CHEMICAL REACTION INVOLVED


NAHPO 4 + 12(( NH ) 42MoO4 + 21 HNO3 +3 H +¿¿( NH 3)3 PO 4.12Mo03 +
21 NH 4NO3 + 12 H 2O
TEST FOR ALCOHOL
EXPERIMENT
Small samples of each brand of cold drinks were taken in separate
test tubes and Iodine followed by Potassium Iodide and Sodium
Hydroxide (NaOH) solution was added to each test tube. Then the
test tubes were heated in hot water bath for 30 minutes.
Appearance of yellow colored precipitate confirmed the presence of
alcohol in cold drinks

OBSERVATON
SR.N NAME OF THE OBSERVATION CONCLUSION
O DRINK
1. COCA COLA YELLOW ALCOHOL IS
PRECIPTATE PRESENT
2. SPRITE YELLOW ALCOHOL IS
PRECIPTATE PRESENT
3. LIMCA YELLOW ALCOHOL IS
PRECIPTATE PRESENT
4. FANTA YELLOW ALCOHOL IS
PRECIPTATE PRESENT

INFERENCE
All the cold drinks samples gave positive test for alcohol. Hence all
the cold drinks contain glucose.

CHEMICAL REACTION INVOLVED


CH 3 CH 2OH + 4 I 2+ 6NaOH  CHI 3 + HCOONa + 5NaI + 5 H 2O
TEST FOR SUCROSE
EXPERIMENT
5 ml samples of each brand of cold drinks were taken in separate
china dishes and were heated very strongly until changes occur.
Black coloured residue left confirmed the presence of sucrose in cold
drinks.

OBSERVATON
[Link] NAME OF THE OBSERVATION CONCLUSION
. SRINK
1. COCA COLA BLACK RESIDUE SUCROSE IS
PRESENT
2. SPRITE BLACK RESIDUE SUCROSE IS
PRESENT
3. LIMCA BLACK RESIDUE SUCROSE IS
PRESENT
4. FANTA BLACK RESIDUE SUCROSE IS
PRESENT

INFERENCE
All the brands of cold drinks contain sucrose. But amount of sucrose
varies in each brand of drink. Fanta contains highest amount of
sucrose.
RESULT
After conducting several tests, it was concluded that the different
brands of cold drinks namely:

1. Coca Cola

2. Sprite

3. Limca

4. Fanta

All contains glucose, alcohol, sucrose, phosphate and carbon dioxide.


All cold drinks are acidic in nature. On comparing the pH value of
different brands Coca Cola is the most acidic and Limca is least acidic
of all the four brands taken.

CARBON DIOXIDE
Among the four samples of cold drinks taken, Sprite has the
maximum amount of dissolved carbon dioxide and Fanta has the
minimum amount of dissolved carbon dioxide.
CONCLUSION
DIS-ADVANTAGES OF COLD DRINKS
1. Soft drinks are little more harmful than sugar solution. As they
contain sugar in large amount which cause problems in diabetes
patients.

2. Soft drinks can cause weight gain as they interfere with the body’s
natural ability to suppress hunger feeling.

3. Soft drinks have ability to dissolve the calcium so they are also
harmful for our bones.

4. Soft drinks contain “phosphoric acid” which has a pH of 2.8. So


they can dissolve a nail in about 4 days.

5. For transportation of soft drinks syrup the commercial truck must


use the hazardous matter place cards reserved for highly consive
material.

6. Soft drinks have also ability to remove blood so they are very
harmful to our body.

USES OF COLD DRINKS


1. Cold drinks can be used as toilet cleaners.

2. They can remove rust spots from chrome car hampers.

3. They clean corrosion from car battery terminals.

4. Soft drinks are used as an excellent ‘detergent’ to remove grease


from clothes.

5. They can lose a rusted bolt.


BIBLIOGRAPHY
LABORATORY MANUAL OF CHEMISTRY BY- Full marks

DINESH COMPANION CHEMISTRY BY- S.K. MALHOTRA

[Link]

[Link]

Common questions

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Benedict's reagent and Fehling’s solutions are used to confirm the presence of glucose by identifying reducing sugars. When a cold drink sample is heated with Benedict's reagent, the formation of a reddish precipitate confirms glucose's presence . Similarly, Fehling's solutions cause a reddish-brown precipitate upon heating, indicating glucose . The presence of glucose is significant because it is a primary source of energy but, when consumed in excess as in soft drinks, can contribute to health issues such as obesity and diabetes .

The primary chemical components found in the brands of cold drinks analyzed are glucose, carbon dioxide, sucrose, alcohol, and phosphate ions. These components are tested using different chemical reactions: Glucose is confirmed using Benedict's reagent and Fehling's solutions, where a reddish or brown precipitate indicates its presence . Carbon dioxide is detected by passing the cold drink through lime water, which turns milky upon reaction . Sucrose is confirmed by heating the drink until a black residue forms . Alcohol is detected with iodine, potassium iodide, and sodium hydroxide, resulting in a yellow precipitate . Phosphate ions are confirmed by adding ammonium molybdate and nitric acid, leading to a canary-yellow precipitate .

The acidity level in cold drinks contributes to their tangy taste, which is often marketed as refreshing and invigorating, appealing to consumers seeking lightness and a burst of energy. For instance, the low pH levels corresponding to high acidity can be associated with a strong tangy flavor that some customers prefer . However, growing awareness of the health implications of consuming acidic beverages, like dental and gastric concerns, could adversely affect consumer perception and reduce their appeal to health-conscious individuals . Marketing strategies focus on immediate sensory experience rather than long-term health consequences, balancing between enjoyment and potential drawbacks .

The presence of alcohol in cold drinks is indicated by the chemical halogenation reaction, where iodine, potassium iodide, and sodium hydroxide react to form a yellow precipitate after heating . Although the alcohol content is likely minimal, its presence raises concerns about unintentional consumer exposure, particularly for individuals sensitive to alcohol for health, religious, or personal reasons. Additionally, the implication is significant for marketing and regulatory aspects, requiring labeling and disclosure to inform consumers adequately .

The test for phosphates involves adding ammonium molybdate and concentrated nitric acid to the cold drink, and heating the mixture. The appearance of a canary-yellow precipitate indicates the presence of phosphate ions, which are present in all the drinks tested . Phosphates have dietary significance, as they are involved in energy storage and release in the body. However, excessive consumption via beverages could disturb natural phosphate balance, potentially affecting bone health and metabolic processes . The presence of phosphates in acidified systems also raises concerns regarding their effect on tooth enamel and general digestive health .

Citric acid and phosphoric acid contribute to the acidity of cold drinks, which affects their pH value. The experiments showed that these acids lower the pH of the drinks, making them more acidic, as evidenced by the color changes on pH paper . The presence of these acids is also responsible for the tangy taste of the drinks. Coca Cola had the lowest pH, indicating the highest acidity, while Limca had the highest pH, indicating the least acidity among the tested drinks .

Carbonation enhances the perceived taste of cold drinks by contributing to a tangy sensation. This is corroborated by the test for carbon dioxide, where carbon dioxide forms carbonic acid when dissolved in water, enhancing the tangy flavor . The experiments demonstrated that carbon dioxide is present across all brands tested, with Sprite having the highest concentration, which may contribute to its stronger tangy taste compared to the others .

Cold drinks pose several health risks due to their chemical composition. The high sugar content from glucose and sucrose contributes to diabetes and weight gain by interfering with the body's hunger suppression mechanisms . The presence of phosphoric acid, with a pH as low as 2.8, can erode calcium in bones and other tissue, increasing the risk of bone health issues . Alcohol traces, though minimal, combined with other chemicals may have cumulative undesirable health effects. The acidity from citric and phosphoric acids can cause dental erosion and other digestive health issues .

Sucrose was detected in all cold drink samples, identified by the residue left after heating . Its presence is significant as it accounts for the sweet taste of soft drinks, enhancing consumer appeal but also contributing to calorie intake and potential weight gain. The variation in sucrose content among brands, with Fanta containing the highest, affects both taste profiles and caloric intake, potentially influencing brand preference based on sweetness levels . This variation can impact marketing strategies that capitalize on the balance between sweetness and health appeal .

From a chemical perspective, cold drinks have both practical household uses and significant drawbacks. Practically, they can act as cleaning agents due to their acidity; for instance, they can clean toilet stains, remove rust, and clean corrosion from car battery terminals . However, these same acidic properties pose health risks. The high acidity can erode dental enamel and contribute to bone health issues due to the dissolution of calcium in soft tissues . They also contribute to obesity due to their high sugar content, challenging overall health when consumed frequently .

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