5100 TABLE-TOP CENTRIFUGE INSTRUCTION and SERVICE MANUAL READ THIS MANUAL CAREFULLY BEFORE OPERATING THE CENTRIFUGE Do not distribute this manual within the U.S.A. and CANADA as the Products acvertised in the manual shall not be distributed in the U.S.A. and CANADA, p. Printed in Japan KUBOTA CORPORATION 29-9 HONGO 3-CHOME. BUNKYO-KU, TOKYO 113, JAPAN 230510104WARRANTY, Kubota Corporation (“Kubota”) warrants that the instrument covered by this warranty shall be free from defects in material and workmanship under normal use. Kubota will repair or replace, free of all charges, the instrument which, within one (1) year after delivery or fifteen (15) months after shipping, whichever comes earlier, is proved to the satisfaction of Kubota to have been defective at the time of delivery, provided that it does not fall under the exceptions and conditions specified in this warranty. Such exceptions and conditions include, but are not limited to, failure due to natural wear and tear, accident, negligence, alteration, repair, or operat yn in a manner not prescribed in the Instruction Manual supplied with the instrument. The foregoing expresses Kubota’s sole warranty with respect to the instrument, THIS WARRANTY IS MADE IN LIEU OF ANY AND ALL OTHER WARRAN- TIES AND ALL IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED AND EXCLUDED. Kubota and its authorized dealers will not be liable for any consequential damages, loss or expense arising from the improper use of the instrument. Kubota will not honor any other warranty which may be given by its representative or dealer or otherwise which is different from the warranty given hereunder. This warranty is not assignable and is operative only in favor of the original customer to whom this warranty is originally delivered. Copyright © 1990 KUBOTA Manufacturing CorporationSafety Instructions Connect the ground wire to the ground terminal, To prevent any chance of electric shock, connect ground wire to ground terminal. For details, see to (2-4. Grounding] on page 2-2. Install your centrifuge in the horizontal place For details, see to [2 -2. Place of Installation] on page 2-1. Do not centrifuge the following (1) Flammable liquid The centrifuge does not have the explosion - proof structure. | (2) Biohazardous liquid | centrifuge is not designed | against bichazardous problems. | Lacquer thinner Do not open the top door when the rotor is rotating Keep the rotor and bucket spéd below the maximum speed, The Maximum speed depends on the rotor and bucket strength. An excessive speed may cause a damage of the rotor and buckets. Mount the buckets in all places to balance the rotor. For details, see Section 5 [Caution in operation] of the Rotor and Buckets. y { a \Table of Contents Section 1. Component Name and Explanation 1-1 s1-2 1-1. Appearance -- +++ 1-2, Control Panel « Sec n 2. Installation and Power Supply 2-1. Unpacking a 2-2, Place of Installation:++-- 2-3. Power Requirements: Grounding -- cesses seen : Assembly and Installation of Centrifuge Table ---~ 2-3 Section 3. Operation Preparation -- = Cautions in Operation »-++++++++++++ - Opening and Closing the door +++++++++ . Turning on the power and opening the door:--- = Opening the door during power failure ------- bGissing tha Vacoe ust en AF, CBee. Calculating Centrifugal Force - Allowable Speed and Load -- « Setting the Speed - - Setting the Timer-- - Saving the Program- « Operation: ++++++++ Alarm Indicators eo 1 NOT hOwH ene ® 1 Section 4. Specifications A= 1, Centrifuge :+---+0+0ecssesorecssseseisonssersesscesseeseceete dB 4-2. Standard Accessories «++++-- vritererreeeereeee daSection 5. Rotor and Buckets « Mounting the Rotor ++++++++++ .RS~4/6 Swinging bucket rotor - . RA - 2886 Angle rotor: RS - 720 Swinging bucket rotor - RS - 96/6 Swinging bucket rotor RA - 3606 Angle rotor RA -500/6 Angle rotor Section 6. Service 6-1, 6-2. 6-3 6-4 6-5 6-10. 6-11 Section 7. -1 . Greasing: - Cleaning of Rotor, Buckets . Cleaning of Chamber-- . Removal and Installation of Chamber « . Inspection and Replacement of Carbon Brushes - Daily Inspection - Monthly Inspection Annual Inspection - Cleaning: Sterilization - Inspection and Replacement of Fuse Adjustment - Troublesho« 1 AHAHDAHRHHOHHOOD 1 AOTAAHONVNYONMEE Parts List and Location Recommended Spare Parts --Section 1. Component Name and Explanation 1 — 1. Appearancea2. «Control! Panel “TIME” knob This knob allows setting the centrifuging time. “SPEED” knob) ‘This knob allows setting the speed. Program selection keys ‘These Keys allow the selection of saved programs. Pressing the key will light the lamp. "NORMAL" key Press this key when the programmed operation is not required. Parameters can be set with each knob and key. Pressing this key during operation will indicate the current parameter status fon each display for § seconds, "START" key, [The lamp tights during This Key is used when a program is saved or parameter to be set is selected. “ENTER” key, This key is used to input parameters selected by the FUNCTION key and set by knobs or Keys, into a program to be stored. “STOP” _ key Press this Key to discontinue operation, Pressing ths ey anaes te ny rng operation Wl ght the lao and smear aces f centrifuging tine, discontinue the operation ‘This lamp flashes when the rotor has come to a ‘complete stop. If the door lock release knob is tured clockwise while this amp is lit or flashing, the door can bbe opened. When the door is opened, this lamp will remain lit. Tiss Bey Sanees the RAPD = SOW oF vice "changeover key,Section 2 Installation and Power Supply 2 —1. Unpacking When the centrifuge is taken out of a corrugated carton box, check the following, © Examine it for any visible damage caused during transportation. If any is found, contact the dealer immediately. ® Confirm that all the accessories listed in [4 ~ 2. Standard Accessories) are included with the delivery. @ The locale dealer will install the centrifuge. 2— 2. Place of Installation Horizontally @ Install the centrifuge on a horizontally placed solid table or stand. @ Avoid places where vibration is transmitted from other location or an inclined place. @® Do not install the centrifuge in places where it may be exposed to direct sunlight or high temperatures. Also avoid places where the ambient temperature is extremely high (40 °C or above) or extremely low (10 °C or below) @ Do not install the centrifuge in a places with high humidity (relative humidity 85 % or above) or in dusty places, 2-12—3. Power Requirements Se ee Prepere a power supply that meets the following conditions. @ The power supply voltage must be the same as that indicated on the nameplate of the centrifuge and the voltage variation must be within the range given in Tabie 2-1 @ The current capacity must be more than 15 amperes. D The outlet must have a ground terminal and its ground resistance must be less than 100 ohm. Use single ~ phase supply Je connecting the cord, install a knife switch or circuit breaker of 15 amperes rating. When the centrifuge is connected to an outlet by means of a plug, use a 3- pin pleg with a ground terminal of 15 amperes rating. Table 2-1 Rated Power supply Voltage and Acceptable Voltage Range Eset Power supply Volissd]_ioov_[ tiov_[ iisv | ov] 200v_|_zeov | aa0v | aw0v i - S0V to] $V to [104V to| I0aV to] T00V to} 198V to] zoTV to] 216V to [Asmat Voltage Rangel! “tiov | iaiv | rev. | isav-| zov | 24a | asav | 2eav 2—4. Grounding ~ Te prevent a chance of electric shock, connect the ground wire of the power cord to the ground terminal, ~ The ground terminal (identified by symbot EWS) ) locating on the rear panel should also be connected to the ground wires: |e— Knife switch || * Ground terminal ° Gas piping Caution : Do not use water pipes for grounding. Ground wire (Green ~ and — yellow Stripe pattern) Do not connect the grounding wire to any of the following: 1. Gas piping 2. Telephone wire 3. Lightning rod ground wire2—5. Assembly and Installation of Centrifuge table (1) eee 1. Standard accessories The standard accessories for the centrifuge table are listed below. Ensure that all sems are available before installation. D Adjuster Adjuster locking nut (M 10) Antiskid holder for rubber leg - Antiskid holder mounting screw (4 mm) Antiskid holder mounting nut (4 mm) tiskid holder mounting plain washer (4 mm) Centrifuge fixing bolt (M 8) S Plain washer (8 mm) 2 Assembling method (1) Pes MMB OWE VSsAe eA Pa a @ Install adjusters on the bottom four corners of the centrifuge table. @® Open front door of the centrifuge table. Install the four antiskid holders onto the four holes at A of top plate using screws, nuts and plain washers. Antiskid holder Screw (4mm) Fiain_ washer (4mm) Nut (4mm) @ Position the centrifuge on the table so that the rubber leg of the centrifuge are positioned in the center of the antiskid holders. Centrifuge table 2-32 —5. Assembly and Installation of Centrifuge table (2) Centrifuge body ean and plain washers from the underside of fa the table into the bottom of the a centrifuge, and tighten it. Top plate Plain washer (8mm) (Centrifuge fixing bolt) 3. Horizontal adjustment method Caution : Make sure to adjust the level of the centrifuge is adjusted with the adjusters. Adjustment procedure is as follows: @ Insert the two centrifuge fixing bolts @ Open the door, install the rotor on the motor shaft, and place a level on the rotor. Caution: Place the level on the rotor. Leveling cannot be performed correctly if the level is placed on the door, for example. @ Adjust the centrifuge to a horizontal position in both left to right and front to rear directions by turning the lower nut of the centrifuge table adjuster. @ Turn the lock nut counterclockwise to shift it upward, then tighten it so that adjuster will not loosen. @ Bach adjuster and should be as shown in the figure on the left after the locking nut is tightened. => —Canae)Section 3 Operation 3— 1. Preparation 1. When operating the centrifuge for the first time. When the centrifuge is put into initial operation after delivery, ensure that the reset switch is pressed without fail after the "POWER" switch is turned on. ‘The figure at below indicates the location of the reset switch. Press the reset switch with the point of a pencil, or a similar object. 2 If the centrifuge does not start while the control panel displays normal. off the "POWER" switch, Wait 5/6 seconds and turn it on again. If the fuge still does not start, press the reset switch, ne centrifuge has been unused for a long time. having been unused for a long time, if the centrifuge will not start even if the “POWER” switch is turned on, the backup battery mounted on the centrifuge may be flat. Follow the same procedure described in article 2. Caution: Pressing the reset switch will delete all programs which have been stored under each key "PRG 1","PRG 2”, "PRG 3” When the reset key is pressed, it is necessary to reenter and save programs.3- 2. Cautions in Operation” 10. rT Do not open the door while the rotor is rotating. Always operate the centrifuge at a speed below the maximum allowable speed, The maximum allowable speed of the rotor has been determined by the strength of the rotor, bucket, etc. Therefore, if the centrifuge is operated at a speed above '@ maximum speed, it may cause damage to the rotor, bucket, etc. other trouble in the centrifuge. Always mount four buckets in position (two buckets for the RS — 96SA//6 rotor). Always place tube racks in a bucket. Even if only two pairs of tube racks are used, place empty tube racks in empty buckets. Balance the loads (sample, bucket, tube rack, cushion, etc.) before operation, If the centrifuge is operated with the load unbalanced, the imbalance detector is actuated and the centrifuge stops operation, Rebalance the loads. De not force to rotate the sensitive "SPEED", and "TIME" knobs. Do not use buckets and tube racks of a size other than specified or those of other manufacturers, Since they may be dislocated or damaged during operation. if a tube in a bucket breaks, replace the cushion with a new one. Hf the cushion is used with fragments of broken glass in it, the tube will be liable to break. Do not centrifuge the following : 1) Flammable ii The centrifuge does not have the explosion ~ proof structure, 2) Bichazardous liquid ‘The centrifuge is not designed against biohazardous problems, If the centrifuge is to be moved to another location, remove the rotor from the motor shaft before transportation. Do not modify the centrifuge or the rotor, nor use parts other than those specified,3-3. Opening and Closing the door (1) 5 1. Turning on the power and opening the door EN /, 4 (1) Turn on the "POWER" switch. Ne (2) The lamp on the control panel will light. STOP (3) Turn the door lock release knob. T 8 The door can be opened. ey & / Door lock release knob Caution: The door lock release knob will hit a stopper when it is turned approx. 90 degrees. Do not force it any further. The door is locked in following cases and will not open even if the door lock release knob is turned. 1. When the rotor is running. 2. When there is a power failure or the power is turned off. When the alarm indicator < OPEN > is lit, the centrifuge can not be started. Close the door firmly.3-3. Opening and Closing the door (2) 2. Opening the door during power failure (1) Turn off the "POWER" switch. (2) Remove the circular rubber plug located on the left side face of the centrifuge. ZL (3) Insert the screwdriver as shown in the figure at left, and move the interlock block to the other side. (4) In that condition, turn the door lock release knob clockwise. The door can be opened. Close the door by pressing on the front edge of the door. If the rotor does not start when the "START" key is pressed, press the door securely and lock it. The door can be closed even when there is a power failure or the power is turned off. Caution :3-4. Calculating Centrifugal Force You can readily calculate the centrifugal force by assigning the Rotation Radius and speed in the following equation ‘Speed_N_ (rpm) Bese tae = tue x Cexriusn force ROF (x #) sux aN 2 ) X Rotation Radius (em) ~ (amu) 3-1 For the Rotation Radius, see page 5-3 and following, and refer to appropriate Specification Table. —5. Allowable Speed and Load ~ When the gravity of sample is 12 or higher or when using a special glass tube 'S used, check the allowable load of the bucket. — E the load exceeds the limit, calculate the calibrated maximum speed of Expression 3-2 and set the actual speed below the calibrated maximum speed. ~ Regarding allowable load on rotors and buckets at the maximum speed, see page SS and following, and refer to the appropriate Specification Table for rotor in use. — The allowable load includes the weight of sample, buckets, cushions, adaptors, tubes, eees and racks. A » id HGR). om) Stes sximum speed (rm) = Maximum speed (rom) x / Aliza Teed Te)... (formal — 6. Setting the Speed ‘Press “NORMAL” key and have the current parameter displayed on the "SPEED” Gspizy i ‘The "SPEED" display indicates the value in units of 100 rpm. Kno} oO Seed Jf) ito “ © ‘SPEED ‘@ Tsang the "SPEED" knob changes the value displayed. Set to the speed as segred. ‘Tis speed can be set from 200 rpm to 5000 rpm in intervals of 100 rpm. ‘Ween RA - 2886 and RA~500/6 are used, the speed can be set from 200 rpm % 6000 rpm in intervals of 100 rpm. 5 2] Sie Oo Spee | 3-5at: Setting the Timer 1 Press “NORMAL” key and have the current parameter displayed on the *TIME* Gspizy. ° NORMAL ée 3 ‘@ Pressing "MIN/SEC” changeover key changes the settable range of centrifuging me as described below. Set to the range as required. At the time of factory shipment, the range is set from 1 to 90 minutes. Setting minutes Settable range 1-90 minutes Setting unit: 1 minute ony Press "MIN//SEC” changeover key Ose Setting minutes Settable range : 10-900 minutes Setting unit: 10 minutes Soin Press "MIN/SEC” changeover key CE Setting seconds Settable range : 1-90 seconds Setting unit : 1 second OMiN m4 Press "MIN/SEC” changeover key seo Setting seconds Settable range: 10 ~ 900 seconds Setting unit: 10 seconds OMIN Press "MIN/SEC’ changeover key sect 6 @ Tarning the “TIME” knob Gres che indication on the display. Set to the time required. S—8. Saving the Program , (1) —The perameters necessary for operation (speed, centrifuging time, acceleration“ Geceieration, etc.) can be stored as programs under either PRG 1, PRG 2 or PRG ‘3 program selection keys. Swered programs can be easily called up by pressing the appropriate program seiecticn Key, enabling repetitive operation under the same set parameters. —eex if the power is turned off, the parameters set remain stored. Fhe procedure for storing programs are described below. = Let us store the following parameters under the PRG 2 key. @ Speed: 5000 rpm Centrifuging time: 85 seconds “ACCEL” < RAPID > acceleration “DECEL™ : < SLOW > deceleration > If either digital display ("SPEED” or "TIME" display) is flashing, the centrifuge can not be started. Press the program selection key being used to stop the flashing. ft is possible to stop flashing by pressing the "ENTER" key. However, the parameter, which has been flashing, changes to the value set by the corresponding knob. When any parameters are changed, press the "ENTER" key. Otherwise, they are not stored in the program. n of program selection key 1. Press "PRG 2” key. The parameters which are currently stored [Fas under te "PRG 2” Key are shown on their y respective display and key. ‘the speed 2 Press “FUNCTION” key. = "SPEED" display flashes rpm indicating the currently a <é Foc Lie 4} *NGQ stored value SPEED & Ef the speed setting is not going to be changed, proceed to Operation 6 Turn the "SPEED" knob S g rem >\ until the display indicates y + xiao 50. SPEED3-8. Saving the Program, (2) Operation 5. Pressing the "ENTER" key allows 5000 rpm, the value set in Operation FUNG 4 to be stored. (Fe 59 rpm "SPEED" display now remains ENTE lit. os ‘SPEED Saving the centrifuging time Oceration 6. Press "FUNCTION" key. MIN " TIME ” display —_ flashes Re fou) Lie OE ) frag | indicating the currently stored vale yy ose 7 v fine OGoestion 7. If the centrifuging time is not going to be changed, proceed to Operation 11 Gceston 8. Is the settable range of centrifuging time shown in seconds (settable range 1-90 seconds) ? If $0, +++++++++ proceed to Operation 9. es © If not, ++++++++ press "MIN/SEC” changeover key until seconds indication (settable range 1-90 seconds) is shown. 3 Turn "TIME" knob until 85 is 8 5\ En") displayed. TIME 10. Pressing the "ENTER" key allows 85 seconds, the centrifuging time set in Operation 9. to be stored. SION "TIME" display now : Bs remains it > TIME ‘De acceleration//deceleration characteristics 11. Press “FUNCTION” key. Either or lamp on "ACCEL”,"DECEL” keys flashes showing the acceleration or deceleration characteristics currently stored in the program, 3-88. Saving the Program, (3) 12. If characteristics of both acceleration or deceleration are not to be altered, proceed to Operation 15, Let us set the acceleration characteristic to on “ACCEL” key. If the lamp is flashing, proceed to Operation 14. Figure shows "ACCEL" key is| set to acceleration. If the lamp is flashing, change to < RAPID > by pressing the "ACCEL" key. = Figure shows "ACCEL" key is set| to acceleration. 14 Let us set the deceleration characteristic to on "DECEL” key If the lamp is flashing, proceed to Operation 15. Figure shows "DECEL” key is| set to acceleration. If the lamp is flashing, change to by pressing the "DECEL” key. Figure shows “DECEL" key is| set to acceleration. 15. Pressing the "ENTER" key allows the acceleration/deceleration characteristic of "ACCEL" - & "DECEL” ~ , which were set in Operations 13. & 14. to be stored. ‘The lamps in the “ACCEL” & "DECEL” GZRAPID) keys now remain lit. ‘ACCEL O stow 16. With the above procedures, the parameters of the have been stored under "PRG 2” key. When the parameters for "PRG 2” are further changed, go back to Operation 2.Operation (1) 1. Turn on the "POWER" switch, 2. After the lamp flashes or lights, open the door by turning the door lock release knob clockwise. 3. Install the rotor. Refer to "5—1. Mounting the Rotor” on page 5-1. 4. For swinging bucket rotor, mount buckets and tube racks on the rotor. 5. Place the sample in the rotor or buckets. Refer to the operating cautions of the relevant rotor described in [Section 5. Rotor and buckets]. 6. Close the door firmly. lamp goes out. 7. Press “NORMAL” key on the control panel. the desired value has been stored in a program, call up the program by pressing the relevant program selection key. When the program has been called up, press the selection key and proceed to Operation 10. 8 Se: desired speed with the "SPEED" knob and desired centrifuging time with the "TIME" knob respectively. © Set braking force during deceleration with the "DECEL” key and acceleration speed with the "ACCEL" key. 1. Press the "START" key. Lamp on the "START" key lights. ond 15 g OMIN Value on the "TIME" display [ 0 starts decreasing. TIME g ain When the value becomes 0, the | ee centrifuge automatically decel ~ cae erates and stops. 3-103—9. Operation (2) %* In the following case ** * * When you want to halt operation, Press the "STOP" key on the control panel. The "STOP" key lamp will light and the rotor will come to a halt. When the rotor has stopped, the lamp flashes and an electronic buzzer sounds. Operation 11. Open the door by turning the door lock release knob clockwise, lamp remains lit. Operation 12. Remove the sample. Operation 13. If the centrifuge is to be used again, return to Operation 4. Operation 14. Turn the "POWER" switch off to cut the power supply. If the power is turned off in while a Program being called up and is then turned on again, the same program as displayed before the Power cut will be called up and displayed. : Turn off the power while "PRG 2” called up. Turn on the power. The "PRG 2” key lamp will light and the parameter stored under “PRG 2° key will be displayed. aua trouble occurs during operation, alarm indicators on the left of the control = flash and indicate the abnormality. any of the “OPEN”, “IMBAL”, “SPEED”, and “ERROR” alarms turns on @uricz operation (while the rotor is rotating), the rotor immediately starts Geceierating and stops. — When an abnormality is indicated, follow the procedure given below. When the seéicctor is flashing, the centrifuge can not be operated. PEN This indicator lights when the door is oper. When it is lit, the centrifuge can not be operated. It flashes and the rotor stops if the door is opened during operation. If it flashes, contact your local dealer for inspection. IMBAL This indicator flashes and the rotor stops when abnormal vibration is detected. 1. Check the balance of the loads (samples, buckets, etc.). Balance them if they are unbalanced. 2. Check that the buckets swing smoothly. Turn off the power after this check. Turn on the power again. If the indicator still flashes, contact your local dealer for inspection. PEED This indicator flashes or the rotor stops when the speed of the rotor exceeds 5300 rpm for swinging bucket rotor and 6300 rpm for angle rotor. Confirm the above. Turn off the power after the rotor is completely stopped. Turn on the power. If the | indicator still erroneously flashes, contact your local dealer for inspection. a This indicator flashes and the rotor stops when the rotor does not rotate within 10 seconds after the "START" key has been pressed due to an abnormality in the electrical circuit. After checking the flashing, turn off the power. Turn on the power again. If the indicator still flashes, contact your local dealer for inspection. 3-124-1 Centrifuge Section 4 Specifications Maximum speed Maximum relative centrifugal force Maximum loading capacity Speed control System Speed setting range Speed indication Centrifugal force indication Timer setting range Rotor acceleration changeover Rotor deceleration changeover Brake system Drive system Emergency indicator and stopon Rated voltage and Rated current Power consumption Total heat produced Dimensions Weight 6000 rpm 4830 Xe 1000 me Microprocessor control From 200 rpm to 6000 rpm Digital. in units of 100 rpm. Digital. in units of 100 X g From 1 second to 90 seconds 10 seconds to 900 seconds I minute to 90 minutes 10 minutes to 900 minutes Rapid, Slow Rapid, Slow Microprocessor control DC high torque motor by Microprocessor control Door Open, Imbalance, Over Speed 100 V 50/60 Hz 7A 110 V 50/60 Hz 7A 115 V 50/60 Hz 7A 120 V 50/60 Hz 7A 220V 50/60Hz 3A 230V 50/60Hz 3A 240. V 50/60 Hz 3A 520W(100~120V MODEL), 330W(220~240V_ MODEL) 450 Keal(100~120V MODEL). 280 Keal(220~240V MODEL) 43 cm Wide, 34 em High, 54 cm Deep 43 kg |. Instruction manual cseeess] Copy 4—2. Standard Accessories 2, 6-mm Allen wrench:NEE NOOO Section 5 Rotor and Buckets 5 —1. Mounting the Rotor (1) Mounting the RS- 720 rotor 1. To install the rotors, match the rotor slot with the joint pin of the motor shaft, 2. Fix the rotor by tightening the rotor bolts. Rotate the bolts clockwise using the allen wrench, Rotor yoke i 3. After installation of the rotor, hold the rotor yoke and attempt to move it up and down to ensure that there is no play. t Motor shaft Caution: Make sure that the rotor yoke is firmly fastened to the motor shaft with the rotor locking bolt. Joint pin Center axis of rotor Caution: If the rotor slot interfere with the joint pins, the rotor locking screw can Not be fastened. In such @ case, reinstall the rotor, Fig.5~1 Installation procedure of rotor (1)