Name: Haydee N.
Paras Score:___________________
Section: BS BIO 3-3LAB B Date: ___________________
DEVELOPMENT OF INCUBATED CHICKEN EMBRYO
A. Egg Candling Pictures
Day 2
Day 1
Day 3 Day 4
Day 6
Day 5
Day 7 Day 8
Day 10
Day 9
Day 11 Day 12
Day 14
Day 13
Day 15 Day 16
Day 17
Day 18
Day 19
Day 20
B. Observable Changes in the Chicken Embryo
Structure Incubation Period (Hours)
24 33 48 72
Membrane Systems The somites are Approximately There are about Embryonic
formed in the 12 pairs of 26 somites membranes
mesoderm somites present A somatopleure amnion, chorion,
Only this head at this time. fold covrs the and yolk sac are
region elevates The vitelline anterior portion prominent
above the veins begin due of the chick’s
underlying area to the signs of body.
pellucida. thickened ridges. The amnion
The notochord in The blood islands which lies next to
the region of the are very visible in th embryo, and
differentiating the vasculosa chorion which
foregut can be region. lies beyond the
seen. There is a amnion, form 2
presence of separate
proamnion. coverings.
Position of the Head The head fold has The head fold is Twisting or The head which
become even even broader and torsion is almost twisted
more bends inward happened when downward, has a
pronounced, marginally over the head fold number of
resembling a the notochord’s emerges from conspicuous
flattened finger- anterior end. the blastoderm. bulges that lead
shaped structure As result, the to brain
protruding posterior part of divisions.
forward from the the body is in its
blastoderm, initial location,
starting at the ventral side
level of the down. The tail
anterior fold is being
intestinal defined at the
extreme caudal
end in almost th
same way as the
head fold was
defined.
Size of the Head (mm) Not observed 0.7mm 1.7mm 1.6mm
Size of the Body (mm) 2mm 4mm 6mm 7mm
Weight of the Embryo (g) n/a n/a n/a n/a
Amount of Amniotic Fluid (ml) Not observed Not observed 2ml 4ml
C. Indicate other distinguishing characteristics of the embryos incubated at different hours.
• 24 hours of incubation
Chick is still not visible, but the yolk is still visible, and the growing membrane
system is somewhat visible and detectable.
• 33 hours of incubation
The head and the body of the chick is still undeveloped, as well as its wings and
beak. Large bulging similar to an optic organ can be seen; The membrane is thick and
visible.
• 48 hours of incubation
The head and body are almost completely developed; The wings, legs and beak are
evident; feathers are dense and almost surround the body; The membrane is thin.
• 72 hours of incubation
Good body dimensions, nearly full body structures, dense fur that covers a
substantial portion of the chick, less yolk, and a thin and delicate membrane layer.
D. Attached pictures of cracked incubated eggs from day 1-21.
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7 Day 8
Day 9
Day 10
Day 11 Day 12
Day 13 Day 14
Day 15 Day 16
Day 17 Day 18
Day 19
Day 20
Day 21
E. ANSWERS TO QUESTIONS
1. What are the observable changes in the chicken embryo development incubated at 24,
33, 48 and 72 hours?
The observable changes in the chicken embryo development incubated at 24 hours are
the membrane system, the position of the head and the size of the body. The changes in
these aspects are also visible in the 33, 48, and 72 hours. However, the amniotic fluid
was just observed after 48 hours of incubation.
2. What are the morphological changes that happen in the chick embryo from the first day
of incubation up to the twenty first day of incubation?
A pointed thickened coating of cells becomes evident in the caudal or tail end of the
embryo soon after incubation starts. The blood islands begin to connect and form a
vascular structure on the second day of incubation, while the heart develops elsewhere.
The beak starts to grow at the end of the third day of incubation, and limb buds for the
wings and legs are visible. Torsion and flexion are still present on the fourth day. The
chick's entire body rotates 90 degrees and it lays flat on its left side on the yolk. Since
the embryo's head and tail are so close together, it forms a "C" pattern. As part of the
digestive and respiratory processes, the mouth, tongue, and nasal pits form. The
embryo has all of the organs needed to survive life after hatching by the end of the
fourth day of incubation, and the majority of the embryo's parts can be established. On
the seventh day, the wings and feet have digits, the heart is entirely embedded in the
thoracic cavity, and the fetus resembles a bird. Feathers and feather tracts become
evident after the tenth day of incubation, and the beak hardens. The claws are emerging
on the fourteenth day, and the embryo is going into place for hatching. The chick is in
the hatching position after twenty days, the beak has pierced the air sac, and pulmonary
respiration has begun. The chick will then continue its breakout from the shell after 21
days of incubation.
