Ste Research 10 q1 Melc 1 Week 1 3
Ste Research 10 q1 Melc 1 Week 1 3
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Research 10 Activity Sheet
Quarter 1 - MELC 1
A Week 1-3
Revisiting the Research Paper
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S REGION VI - WESTERN VISAYAS
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Research 10
Activity Sheet No. 1- Research Plan
First Edition, 2020
Republic Act 8293, section 176 states that: No copyright shall subsist
in any work of the Government of the Philippines. However, prior approval of
the government agency or office wherein the work is created shall be
necessary for exploitation of such work for profit. Such agency or office may,
among other things, impose as a condition the payment of royalties.
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Introductory Message
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Name of Learner: _________________________________________________________
Grade and Section: _________________________Date: _________________________
I Learning Competency
• Hypothesis
o For a more ‘scientific’ or experimental approach, the researcher
may choose to generate and test a hypothesis.
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o A hypothesis is a statement of anticipated behavior, and it is
usually expressed in a ‘null’ form.
• Literature Review
o Do not take too narrow a view of ‘literature’.
o In this context literature may comprise not only scholarly papers;
but also national, local or organizational policy documents; in-
house reports and a range of other project-relevant
documentation.
o The purpose of the literature review can be described as two-fold,
“to acquaint you with current thinking in your subject area, and
to find out about methods and research processes used by other
researchers investigating this topic
o Literature review you should be familiar with the key issues and
theories from your subject area and be able to identify any gaps
• Ethical issues
o If you plan to involve either people or animals in your research
then you will almost certainly need to seek ethical approval for
your work.
o Should describe the main ethical issues likely to arise from the
project and indicate how it will be handled.
o If human participation is involved in the study, an informed
consent and guaranteeing that all data gathered from them will be
held anonymously and confidentially.
o IACUC protocol should be taken into consideration when using
vertebrate animals in the study.
• Risk assessment
o When planning a research project therefore, the researcher must
ask themselves a number of questions:
• What risks might prevent a successful outcome to the project?
• What is the probability of each risk occurring?
• What would be the impact on the project if a risk occurred?
• How will a risk be managed?
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• Resources
o Materials should be available in the locality.
o Identify the facilities to be used (laboratory, clinical, animal,
computer, office, other). If appropriate, indicate their capacities,
pertinent capabilities, relative proximity and extent of availability
to the project.
o Describe only those resources that are directly applicable to the
proposed work.
o Provide any information describing the other resources available
to the project (e.g., machine shop, electronic shop) and the extent
to which they would be available to the project.
o Describe how the scientific environment in which the research
will be done
o If there are multiple performance sites, describe the resources
available at each site.
o Describe any special facilities used for working with biohazards or
other potentially dangerous substances.
• References
o Any documents, websites, or other resources referred to in the
proposal must be correctly referenced.
• Further considerations
o Keep your language straightforward and direct. Try to be concise
and to the point. Short sentences are good. Avoid waffling.
o Develop your ideas clearly and logically. This will help the reader
understand not only what you wish to do, but why.
o Use ‘signposts’ such as headings and linking sentences between
sections to emphasize the flow through the plan.
o Make sure your facts are accurate and that you have covered all
necessary points.
o Emphasize key points, not details.
o Check for typographical errors and spelling mistakes – do not rely
on your computer’s spell-checker since it cannot distinguish
between words with different meanings (such as ‘there’ and ‘their’).
o Avoid jargon. Assume your audience are non-expert and may not
be familiar with your subject matter.
o Avoid the use of too many acronyms, but where unavoidable, write
them in full the first time you use them.
o Avoid repetition.
o Make use of figures and illustrations. A picture can sometimes
convey more information than a lengthy block of text.
o Pay attention to the presentation of your plan. A clear and tidy
plan suggests a clear and tidy approach to work. A messy plan will
not only be more difficult for the reader to follow, it may also be
taken to indicate sloppiness and lack of rigor in research.
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III Activity Proper
2.
3.
Research Problem 1.
__________________________________________________________________________________
__________________________________________________________________________________
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Research Problem 2.
_________________________________________________________________________________
_________________________________________________________________________________
Research Problem 3.
__________________________________________________________________________________
__________________________________________________________________________________
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Among the 3 research problems evaluated, which one got the most number
of YES answer?
(Write the title of the research problem here)
_________________________________________________________________________________
_________________________________________________________________________________
Activity 2. Help!
Directions: Please read the situation below and be able to answer the given
questions.
Daisy has in mind to determine the number of days in the vase and
visible changes in characteristics of roses subjected to coconut water
treatments as well as to compare these data to those data using plain water.
And Daisy is really expecting that Roses under coconut water treatments will
have longer vase-life as compared to roses with only plain water. In addition,
she expected that roses under coconut water treatments will extend the
number of days, with minimum visible changes in characteristics, as
compared to plain water and that coconut water is effective in prolonging the
vase -life of roses.
Guide Questions
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2. Based on the situation above, formulate the following:
A. Research Question
___________________________________________________________________________
___________________________________________________________________________
B. Hypothesis/es
___________________________________________________________________________
___________________________________________________________________________
C. Expected Outcome
___________________________________________________________________________
___________________________________________________________________________
5. What must be done with the results that will be gathered by Daisy in her
experiment?
___________________________________________________________________________
___________________________________________________________________________
6. Where did Daisy got the information needed for her investigation?
___________________________________________________________________________
___________________________________________________________________________
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Activity 3. Let’s Apply
Directions: Given the checklist of criteria below, study and evaluate the
given Research Plan below by checking the box corresponding to
your answer.
RESEARCH PLAN
In vitro Antihelmintic Activity of Lantana camara
Extracts on Eudrilus euginiae
Life Science
A. Rationale
Null Hypotheses:
1. Baho-baho (Lantana camara) aqueous extract using the leaves, young stem, and
flower will not exhibit in vitro anthelmintic activity against Eudrilus euginiae.
2. There is no significant difference on the in vitro anthelminthic activity of varying
concentrations of Baho-baho (Lantana camara) aqueous extract using the
leaves, young stem, and flower in terms of the worms’ paralysis time..
3. There is no significant difference on the in vitro anthelminthic activity of varying
concentrations of Baho-baho (Lantana camara) aqueous extract using the
leaves, young stem, and flower in terms of the worms’ lethal time.
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C. Procedure
The following Regulated Research institutions will be the venue of the
different tests to be conducted in the study:
Hicalde Farms in Sigma Capiz, it will be the site where we will collect the Eudrilus
euginiae and Roxas Memorial Provincial Hospital, it will be the site where the
experimentation will take place.
Plant Material and Extraction. Young stem, leaves and flower (~4-6 months
old) of Lantana camara will be obtained from Brgy. Cagay, Roxas City. Collected
samples will be air-dried for 4 days to retain <10% moisture and properly stored
in sealable plastic bags. A whole plant specimen will be subjected to
identification at the Department of Agriculture.
Air-dried Lantana camara will then be coarsely ground. Lantana camara will
be extracted using aqueous solution according to the recommendations of WHO
for medicinal plants based on the Philippine Pharmacopeia Annex A of A.O. No.
172, s.2004. This will ensure that antioxidant properties will be preserved as
they are more aqueous in nature. About 300g of each ground plant material will
be boiled in 1L distilled water in a beaker for 30 minutes at 100 oC. Then it will
then be cooled to room temperature and filtered through a filter paper.
Collection of earthworms. Seventy-two Eudrilus euginiae, nearly equal in
sizes (~6-8 cm) and weight (~1.5-2.5g), will be collected from the Hicalde Farms
in Sigma Capiz. A sample organism will be submitted for proper identification
at the Department of Agriculture.
Accumulation of earthworms. The collected Eudrilus euginiae will be
cleaned in natural saline solution to remove the soil and to prepare them for
the experimentation.
Preparation of the Different Treatments. Plant extract collected from the
stems will be diluted with normal saline to obtain 50% and 100%
concentrations. Albendazole will be used as the standard drug and will be
diluted with normal saline to obtain 1mg/ml concentration and poured into
Petri dishes. Normal saline (0.9% NaCl) alone will serve as the negative control.
Applying the Different Treatments. Eight groups of earthworms (n = 3/ petri
dish) will be utilized for the study. All treatment dilutions will be poured into
the Petri dishes accordingly containing 20 ml of each concentration.
Earthworms, nearly equal sizes (about 6-8 cm), will then be released in the
petri dishes containing each treatment at room temperature.
In vitro Anthelmintic Activity. The in vitro Anthelmintic Activity will be
performed according to the method of Ghosh et al. (2005) and Goswami et al.
(2011) with minor modifications.
A. Determining the time of paralysis:
Time for paralysis will be noted when no movement was observed with a slight
pin prick method by pricking the worms with a pin to check whether they are
paralyzed or dead. The time of paralysis will be recorded in terms of minutes.
B. Determining the lethal time:
The lethal time will be recorded in terms of minutes after ascertaining that
the worms neither moved when shaken vigorously nor when dipped in warm
water (50°C).
Statistical Analyses. Data obtained will be further analyzed using Two-way
Analysis of Variance (ANOVA) to compare significant differences between
treatments and control. Statistical tool for Agricultural Research (STAR) 2.0.1
was employed. All inferential tests will be set at 5% level of significance.
References:
Ferreira, L. E., Castro, P. M. N., Chagas, A. C. S., França, S. C., & Beleboni, R.
O. (2013). In vitro anthelmintic activity of aqueous leaf extract of Annona
muricata L. (Annonaceae) against Haemonchus contortus from sheep.
Experimental Parasitology, 134(3), 327–332.
https://doi.org/10.1016/j.exppara.2013.03.032
Belizario, V. Y., Totañes, F. I. G., de Leon, W. U., Lumampao, Y. F., & Ciro, R.
N. T. (2011). Soil-transmitted helminth and other intestinal parasitic
infections among school children in indigenous people communities in
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Davao del Norte, Philippines. Acta Tropica, 120(SUPPL. 1).
https://doi.org/10.1016/j.actatropica.2011.02.010
Swathi, H., & Shekshavali, T. (2016). In Vitro Evaluation of Anthelmintic activity
of Rhus mysorensis leaves . Research Journal of Pharmacology and
Pharmacodynamics, 8(3), 115. https://doi.org/10.5958/2321-
5836.2016.00021.5
K, V. K., S, D. S., & Sukumar, E. (n.d.). Bio Chemistry Preliminary Phytochemical
Studies of Lantana Aculeata Roots, 365–368.
Kumar, K. V., Sharief, S. D., & Sukumar, E. (2011). Preliminary phytochemical
studies of Lantana aculeata roots. International Journal of Pharma and Bio
Sciences, 2(2), 365–368.
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Included special facilities for working with biohazards or
other potentially dangerous substances.
References Are all documents, websites and other resources fully and
accurately cited and referenced?
Followed the preferred referencing style?
Other Criteria Is the writing style clear, concise and to the point?
Does the research plan have a logical flow?
Used appropriate headings and other signposts to help the
reader?
Absence of acronyms and jargon?
Where there typographical and spelling errors?
Are figures and illustrations used appropriately and
referred to in the text?
Absence of non-essential text?
Activity 4. Let’s Do It
Directions:
A. From the title you selected in activity 1, make your research plan.
B. Evaluate the research plan you had made based on the criteria given above and
make the necessary corrections.
C. Submit your output to your teacher.
IV Reflection
I realized that
__________________________________________________________________________________
__________________________________________________________________________________
I will apply
__________________________________________________________________________________
__________________________________________________________________________________
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Formulating A Research Plan https://www.ifcc.org/media/410323/05
_Research_ Guide_ IFCC.pdf
Practical Guide in Local Science Fairs Prepared by Elson Ian Nyl Ebreo Galang
Intel ISEF 2012 Finalist Through the initiative of the Network of ISEF
Alumni Philippines (NIAP) First Printed: June 2014
Pickton, M. (2013). Writing your research plan. In: Grant, M.J., Sen, B. and
Spring, H. (eds) Research, Evaluation and Audit: Key Steps in
Demonstrating Your Value. London:facet Publishing
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