2023 JETIR June 2023, Volume 10, Issue 6 www.jetir.

org (ISSN-2349-5162)

IN VITRO PROPAGATION OF AGLAONEMA

(AGLAONEMA COMMUTATUM)
1
Gadhe Swaranjali, 2Kale Abhishek
1
Assistant Professor, 2Student
Department of Plant Biotechnology
College of Agricultural Biotechnology, Loni, (Ahmedanagar), India

ABSTRACT: The present investigation “In vitro propagation of Aglaonema (Aglaonema commutatum),
was undertaken to identify suitable media combinations for in vitro propagation of Aglaonema. The mother
plant of Aglaonema was collected from Mauli Hitech Nursery, Solu, Tal.Khed, Dist.Pune. The nodal
explants were initiated on MS media with 3% sucrose, 8gm Agar fortified with different concentration of
BA and NAA (3.0, 0.5+2.5, 1.0+2.0, 1.5+1.5, 2.0+1.0 mg/l) respectively. After four weeks of inoculations
it was observed that MS media supplemented with 1.0 mg/l BA + 2.0 mg/l NAA gives best result with 80%
initiation. MS media supplemented with 4.0 mg/l BA + 1.0 mg/l NAA was found to be most suitable for
shoot multiplication i.e. average 3 shoots per bottle. MS media supplemented with 0.5 mg/l IBA + 0.25
mg/l NAA was found to be most suitable for rooting i.e. average 5 roots per explants. Thus, the nodal
explant of Aglaonema can be effectively used for the in vitro propagation.
Keywords: Aglaonema, MS media, BA, NAA, IBA.

1. INTRODUCTION
Aglaonema commutatum, belongs to the family Araceae, which commonly known as aroids, it has more
than three thousand species that are mostly herbaceous either as terrestrial, aquatic, or epiphytic (Mayo et
al., 1997; Brown, 2000). The genus Aglaonema is comprised of 21 species which inhabit humid and
heavily shaded forests of many territories of Asia (Chen et al., 2003). Aglaonema is an ornamental plant
important in interior landscaping due to its attractive brightly colored leaves.Aglaonema has been produced
as a foliage ornamental plant due to its attractive foliage, easiness to grow and tolerance to low light
conditions and low relative humidity (Henny, 2000; Chen et al., 2002). The rooting of its cuttings and
division of basal shoots are the basic methods of propagation since non-simultaneous flowering and short
life span of the pollen make sexual reproduction difficult.

Adoption of in vitro propagation has reduced the time-spanned for plant introduction and new cultivar
release. Using micropropagation techniques, a new cultivar can be improved quickly enough to reach
commercial production levels (Henny and Chen, 2003). It has been principally effective mainly due to

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difficulty of establishing sterile culture (Chen and Yeh, 2007), low rate of shoot multiplication (Zhang et
al., 2004), and lack of detailed methodological information on invitro propagation technique (Marianiet al.,
2011).

Need of propagation of Aglaonema : The non-simultaneous flowering and short life cycle of the pollen
make the sexual reproduction difficult, so most of Aglaonema sp. has been propagated by cutting the
rooting part, from nodes, or shoot basal division as the basic method (Barakat and Gaber, 2018).

Micropropagation technique are advanced vegetative propagation technique for producing a large amount
of uniform and pathogen-free transplants in a short period of time and limited space, decreases greenhouse
space needed for stock plant production and provides growers with lines of tissue-cultured plantlets grown
in cell plug trays on a year-round basis (Chen and Henny, 2008).

MATERIALS AND METHODS

Source of explant and sterilization : The Axillary shoots explants were collected from Maulihitech
nursery, Solu and bought to the tissue culture laboratory. These explants were thoroughly washed under
running tap water for 5-6 times. Thenthe explants were washed with few drops of Tween 20 for 15 min.
Then they were treated with 0.5% Bavistin for 4 minutes followed by washing with 70% ethanol for 15 sec
in laminar air flow. Finally it was treated with 0.1% mercuric chloride for 1 min. Then it was thoroughly
washed with sterile distilled water for 4-5 times before inoculation. Finally the explants were dried using
the sterilized tissue paper.

Initiation stage and culture conditions:The surface sterilized nodal explants were inoculated on the MS
media supplemented with different combinations of BA and NAA.All the inoculated cultured bottles were
incubated at 25 ± 2 ºC temperature for 16 hours light (2000-3000 lux) and 8 hours dark period.

Shoot initiation and multiplication and rooting: After four weeks of the growth cycle, established
cultures were subjected to different media concentrations of BA and NAA for multiplication. The shoot
initials obtained from the in vitro established cultures were subjected for multiplication. The shoot number
per explants varied under various cytokinin and auxin concentration. After successful shoot multiplication,
the explants were transferred to rooting media supplemented with varying concentrations of IBA and NAA.

RESULT AND DISCUSSION

The shoot initiations were obtained after 4 weeks of culture inoculation. The best result was obtained
on MS medium supplemented with 1.0 mg/l BA + 2.0 mg/l NAA.

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Table 1:Effect of different concentration of BA & NAA
on culture initiation of Aglaonema after four weeks

Sr. MS Media + BA + NAA Total no. of Total no. Rate of

No (mg/l) explants of explants Survival in %
inoculated shown
BA NAA
growth
1. 0.0 3.0 20 10 50
2. 0.5 2.5 20 14 70
3. 1.0 2.0 20 16 80
4. 1.5 1.5 20 12 60
5. 2.0 1.0 20 10 50

The explants which successfully grown on the initiation medium were then subjected to multiplication medium
supplemented with combinations of BA and NAA. The best results were obtained on MS medium supplemented
with BA 4.0 mg/l and NAA 1.0mg/l. The mean no. of shoots observed 4 on the said combination.

Table 2: Effect of different concentration of BA & NAA

on culture multiplication

Sr.no MS media + BA + NAA (mg/l) Mean no. of Shoots

BA NAA
1. 2.0 2.0 1
2. 3.0 1.5 3
3. 4.0 1.0 4
4. 5.0 0.5 2

After the growth cycle of initiation and multiplication the culture were transferred to rooting media for four
weeks. The combination of MS media supplemented with IBA 1.0 mg/l + NAA 0.25 mg/l shown best
result for the rooting.

Table 3: Effect of different concentration of IBA & NAA on rooting

Sr.no MS media + IBA + NAA (mg/l) Average Number of

roots
IBA NAA

1. 1.0 0.5 3

2. 0.5 0.25 6

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A. Initation

B. Multiplication

C. Rooting

CONCLUSION
From the present study it can be concluded that the nodal segment of Aglaonema can be effectively used
for in vitro propagation of the plant. The hormones like BA, NAA and IBA are useful for the initiation,
multiplication and root formation along with full strength MS medium. Thus the productions of large
numbers of Aglaonema plants are possible through in vitro propagation technique.

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