Andrea Frangiosa, La Merced Tarija, B-38

What is Bio-gas?
Bio-gas, also referred to as digester gas, swamp gas, or marsh gas, is the by product of
decomposing organic matter. Bio-gas is made up of about 1%-80% methane, carbon dioxide,
and trace amounts of nitrogen, hydrogen, and hydrogen sulfide. Bio-gas mixtures containing
more than 50% methane are combustible. The gas that is produced can be used as fuel to cook,
heat, and light households.
Bio-gas is produced when organic material decomposes under anaerobic conditions. This
anaerobic digestion occurs naturally in wetlands, lake bottoms, and deep in soils. Anaerobic
digestion and the production of methane as a by product occurs in a two-stage process due to the
presence of bacteria which thrive in an oxygen deprived environment.

Stage 1
Acid-forming bacteria break down organic matter into volatile fatty acids. Proteins are
broken down into amino acids which are then broken down into volatile fatty acids.
Carbohydrates are broken down into simple sugars and then into volatile fatty acids. Fats and
oils are broken down into long chain fatty acids and then into volatile fatty acids.
Acetogenic bacteria use the volatile fatty acids to form acetic, propionic, and lactic acids;
as well as hydrogen and carbon dioxide gas.
Stage 2
Methane-forming bacteria (methanogens) use the acids formed in Stage 1 from the
breaking down of proteins, carbohydrates, fats, and oils to produce methane. Other bacteria that
are present in Stage 1 use some of the hydrogen and carbon dioxide to produce methane as well.

The production of Bio-gas is initiated by putting organic material into a biogenerator, or

bio-gas digester. The biogenerator provides an environment where the necessary anaerobic
reactions can take place. The biogenerator, or unit, also captures and stores the biogas for latter
use as combustible fuel. The unit produces two by products, the bio-gas and the digestate.
Digestate is the sludge like material left over after the organic material has undergone anaerobic
decomposition. Digestate can be used as an agricultural fertilizer which can aid in increased soil
moisture retention, as well as improved soil fertility.
The biodigestion process does reduce the amount of disease causing pathogens in the
manure. This is especially true if the unit reaches higher temperatures. However, caution still
needs to be exercised when ever working with animal waste, as e coli is present as well as other
bacteria that can cause illness.
Organic Materials that can be used to produce bio-gas.
Manure
* cows, pigs, or chicken
Ideally pig and chicken manure is most affective in the production of bio-gas.
Organic Matter
* agricultural wastes, as long as they are organic
* sugar cane, material left over after the extraction of the cane juice
* straw and material left over from the harvesting of wheat
* corn stalks left over from the harvesting of corn
* weeds from the vegetable garden
* coffee bean shells and pulp
You can use only pure manure, or only pure plant material, or you can mix the two together.
However you will more than likely be able to produce higher quantities of bio-gas if you use
pure manure or manure mixed with a low percentage of plant material.

Why is Bio-gas beneficial?

Why is the production of bio-gas a good idea? There are various reasons why bio-gas is
beneficial to implement, both economically and environmentally.
Here in the rural areas of Bolivia many people use wood as their main source of fuel for
cooking. They often use only wood because buying gas tanks and gas stoves are cost
prohibited. As a result an extra strain has been placed on the surrounding rural environment, as
well as forested areas. The cutting down of trees for a fuel source leads to deforestation and
degradation of forest ecosystems. Gathering wood is often a time consuming task that can
involve walking long distances with heavy loads of wood.
 Take into consideration that in many rural areas food is usually prepared using an open
wood fire in confined roofed areas. These open fires usually have no chimneys and produce
large quantities of smoke. The smoke produced by these fires, in confined closed areas, can lead
to respiratory irritation, infections, and illness.
Another important fact about bio-gas is that it is a renewable energy resource, and is not a
fossil fuel; it is a non-petroleum based product. In other words you are not contibutating to the
further depletion of non renewable resources by using bio-gas as a fuel.
When a Household uses Bio-gas they:
Will use less:
* natural gas, kerosene, wood, and coal
They will become less dependant on these materials as a result.
Due to the time saved by not looking for wood, families will have more time to:
* do domestic tasks
* do agriculture
* do trades such as artesania to help earn more income
* have a little bit more free time to spend with family and friends
Other benefits of using bio-gas
* People will have better air quality within the kitchen because bio-
gas does not produce smoke like wood or coal when burned.
* People will diminish their impact on the environment that
surrounds them by
decreasing deforestation and the use of non renewable resources.
* They will not only produce fuel for cooking, heating, and light,
but they will
also be producing digestate which can be used as organic fertilizer.

How to Build a Small Scale Biogenerator

It must be noted that it is neither easy, nor straight forward to build a biogenerator in
order to produce bio-gas. It is a commitment and investment of time, work, money, and
experimentation. Therefore, it is suggested that you make a small scale unit first to learn how
the system works. There is a level of experimentation involved with this project. Using a small
scale unit will make it easier to find out what works best to produce the largest quantity of bio-
gas from the organic material.
Where to build a biogenerator
Look for a place that is close to the area where you will be using the gas. There needs to
be a distance of at least 10 meters between the area where you are going to use the gas and the
unit. Keep in mind that the larger the distance between the two areas, the more gas hose you will
need to buy, and the more expensive the unit will become to construct. Also the larger the
distance the more gas line you will need to maintain, and repair to prevent gas leaks. Make sure
that the gas line will be in an area that is protected, away from chickens and other animals. This
needs to be done in order to prevent puncturing of the line. This could mean that you will need
to bury the gas line.
The unit needs to be at least 15 meters away from water sources. This is to prevent
contamination of the water by the manure that you will be using in the unit to produce gas.
Biogenerators perform best between the temperatures of 32°C to 37°C. 35°C is the ideal
temperature for anaerobic digestion. As temperature decreases so does bacteria activity, thus
resulting in lower production of bio-gas. As temperature increases to a high level, above 37°C,
some bacteria begin to die and as a result bio-gas production decreases or stops completely.
Since temperature regulation is such an important factor in bio-gas production, it is extremely
important to place the unit in a suitable location.
It is not advisable to place the unit in an area that has direct sunlight all day long,
especially in hot climates. You need a location that experiences a good mix of both sunlight and
shade through out the day. Trying to maintain a unit temperature of 35°C is key and will greatly
aid in the success of the project.
In colder climates it may be necessary to bury the unit in a compost pile above ground.
The compost pile naturally generates heat due to the decomposition of the organic matter present
in the compost. This heat can help regulate the temperature of the unit and avoid allowing the
unit to fall below 32°C, which would lower the amount of bio-gas being produced. It is not
advisable to bury the unit below ground. It makes it more difficult to maintain as well as reload
manure and empty the digestate.
Materials Needed
For the construction of the unit you will need the following:
 One old oil barrel that is at least 200 liters in volume. Look for an oil barrel that is in
good condition, NOT rusted. You want to create an air tight unit, or you will lose the
bio-gas you are trying to produce through leaks from the barrel. Make sure the barrel has
two openings on the top, a larger one that has a screw down cap, and a smaller one that
has a diameter of about 2 cm.

 One metal pipe with the length of 15 cm and the diameter of 2 cm. Make sure the pipe
threaded at one end so you can screw it to the T pipe.

 One metal T pipe with diameter of 2cm.

 Two metal pipes with 10 cm of length, and diameter of 2 cm that are threaded at both
ends.

 2 metal gas valves

  2 hose adapters

 Gas hose that is either made of rubber or plastic and at least 12 meters in length and 2 cm
in diameter. Make sure to take note of the distance between the unit and the stove. Make
sure that the hose fits snuggly to the hose adapters.

 2 or more large inner tubes to store the gas.

 1 small metal T to connect hose to multiple inner tubes.

 A least 7 hose clamps to tighten and secure the hose to the gas exit on the unit.
 Teflon tape to seal connections between pipes.

To make the catalyst you will need:

 A small container that has a volume of at least 15 liters that has a secure screw down cap.
 Rubber or plastic hose with a length of about 50 cm.
 sealing putty
 empty wine or pop bottle

Materials need to prepare unit:

  Welder, or access to a welding shop
 Large container to mix manure and water together. An old oil barrel cut in half is useful
for this project.
 Scrubby brush
 Detergent, Omo works great
 Bucket
 Stick
 Funnel with a diameter equal to the opening in the oil barrel to which you will pour the
manure mixture.

Cleaning the Barrel

You will need to clean the barrel both inside and out, making sure to remove all
petroleum residue. If you do not clean the barrel sufficiently the oil residue could inhibit the
production of bio-gas.
1) First clean the inside of the barrel by heating water in the half barrel container. You can do this
by building a small fire under the container that is propped up on rocks. Pour the hot water into
the large oil barrel when the barrel is in the horizontal position. Then pour powdered detergent
into the oil barrel.

2) Now place the oil barrel with the water/detergent mixture on its side and roll the barrel back and
forth. This will clean the oil residue off from the inside of the barrel walls. Do this until you
think you have washed the inside of the barrel evenly.
3) Pour out the dirty water and repeat the process until the water that you pour out of the unit is
relatively clean.

4) Remember that this is an excellent time to look for leaks on the barrel. If there are leaks, mark
them and fix them by welding them closed. You can do this with or without using a metal
patch. It all depends on the severity of the leak. The unit needs to be air tight in order to
produce and save the bio-gas effectively.

5) Once the inside is clean, pour clean water, without detergent, into the barrel and rinse out all the
remaining soap. It is very important to rinse the barrel thoroughly because the detergent can also
affect the production of bio-gas. The soap could kill the beneficial bacteria you are trying to
cultivate.
6) Now wash the outside of the barrel with hot water and detergent. It is best to scrub the outside
with a brush until all oil residue is cleaned off. Make sure to the clean the top of the barrel well,
especially the two holes that you will use in the future to fill the barrel with manure.

7) Rinse the outside of the barrel well and then turn it upside down, resting the barrel on a few
rocks. This is to help all the water to drain out of the barrel. Once the barrel is dry, inside and
out, you can start the construction of the unit.

Constructing the Unit

1) Take the 15 cm pipe and weld it to the smaller of the two holes on the top of the barrel. This
smaller hole is where the gas produced will exit the unit. The larger of the two holes will be
used to fill the unit with manure. It is important that the larger hole has a cap that you can screw
down tightly. It is very important that the unit be as air tight as possible.
2) After you have welded on the 15 cm pipe attach the T pipe, 10 cm pipe, hose adapters, and the
two metal gas valves as shown in the drawing.

3) Now take the inner tubes and make sure that they are without holes or tears in the rubber. To do
this pump up the inner tube with air and then submerge the inner tube in water. Look for small
bubbles escaping from the inner tube, this signifies air loss.

4) Mark the holes and patch them like you would a bike tire with a rubber patch and cement.
5) Once the inner tube is ready make sure that the inner tube has a two way valve. The valve
should allow gas to enter and leave freely from the inner tube.

6) -Now lay the inner tube flat and roll it up using a stick that is at least the width of the inner tube.
Roll the inner tube using the stick, trying to get as much air out of the inner tube as possible.

7) Clamp a portion of the hose length that you are going to use to attach the inner tubes to the unit,
making sure that no air will be able to pass through the hose.

8) Take this hose and attach the hose to the inner tube, while still rolled up, at the two way valve.
Make sure the connection is secure; you may need to use a hose clamp or wrap wire around the
connection. Repeat the above process with each inner tube and inner tube attachment.
9) Take the inner tubes and secure them with sticks. This is to immobilize the inner tubes. If you
have more than one inner tube you can stack them on top of one another.

10) Take the hose that is attached to the inner tubes and attach it to the unit at the hose adapter. You
are now ready to fill the unit with organic matter.

Making the Catalyst

About a month to two months before you fill the unit you should make a catalyst. The catalyst
will jump start the production of bacteria in the unit and speed up the production of bio-gas
inside the unit.
1) Take the screw cap of the 15 liter container you will be using and punch a hole in the top.

2) Take the rubber hose and secure one end to the screw cap, securing and sealing the sides of the
hose to cap with cement or sealing putty. Make sure that the connection between the rubber hose
and the cap is air tight and that no air escapes.

3) Take 5 liters of manure and 5 liters of water and mix them together in a bucket. Let it stand for
20 minutes, mixing periodically, making sure that the mixture is some what smooth and the
thickness of paint.
4) Then pour the manure-water mixture into the 15 liter container and secure the cap with the hose
attached to it.
5) Take an empty wine or soda bottle and fill it half way with water. Then take the free end of the
hose that is attached to the 15 liter container and place it into the bottle of water. Make sure that
the hose is submerged completely in the water.

6) Leave the manure mixture to ferment for at least 4 weeks. Agitate the container, mixing and
moving the manure, at least 3 times a week. Bio-gas will be produced in small quantities. You
can lift the hose in the bottle and see bubbles being released from the hose when gas is being
produced. You can also experiment and try and light the gas that is escaping from the end of the
hose.

Filling the Unit

1) Using an old oil barrel that is cut in half or any other large volume container, mix one part
manure with one part water. For example, mix three buckets manure with three buckets of
water.

2) Mix in a small amount of catalyst, and let the mixture stand for about 20 to 30 minutes. Mix
every 5 minutes until the mixture is smooth and the consistency of paint.

3) Once the mixture is ready pour it into the unit, repeat steps 1 and 2 until the barrel is almost
full. Slowly add the catalyst to the manure mixture, little by little, until all used up. Make sure
to leave 10 cm between the top level of the manure and the top of the barrel. It is a good idea to
 have the gas valve that runs to the stove open so that you allow air to escape from the barrel
while you are filling it with the manure.
4) Once the unit is full to capacity, secure the unit by capping the larger of the two holes. Make
sure that the main gas valve is closed, the one that leads to the stove. Make sure that all
connections are air tight and you have no leaking of gas.
5) Now you can open the hose that runs from the unit to the inner tubes. This will allow the bio-
gas that is produced to be stored in the inner tubes.

6) To help facilitate the production of bio-gas twice a week you can shake the unit, this turns the
mixture within the unit, which can aid in the production of bio-gas.

Important Information
Production Time
It maybe necessary to wait three weeks to one month until there is any production of bio-gas.
You will note the production of gas when the inner tubes start to inflate. Production of bio-gas
can last for up to eight weeks. Half of the gas will be produced in the first two to three weeks,
and the rest will be produced between the fourth and 8th weeks. If you are producing little to no
gas towards the end of the 6th week, empty the unit and repeat the process again.
Do not try to burn the gas that is produced at the beginning of production. Instead, once the
inner tube is full, open the valves and release the gas. In order to empty the inner tubes you may
need to place some heavy objects on top of the inner tubes, smooth rocks, wooden boards, to aid
in pushing the gas out of the inner tubes.

The first gas produced usually has a high amount of water and air mixed with it; this is why it
is a good idea to release it. Make sure that there are no open flames close to the unit, as this is a
combustible gas that is very volatile. After you release the first batch close the valves and let the
unit fill the inner tubes a second time, you can use this gas as fuel.

Temperature
 Temperature is a key factor in bio-gas production. The best temperatures for bio-gas
production are between 32°C and 37°C. When the unit drops to 15°C, the production of bio-gas
stops completely. As mentioned earlier you can prevent the unit from dropping below the
optimal temperatures by burying the unit in a compost pile. However, it is not a good idea to
bury the unit underground because it makes maintenance and reloading of the unit very difficult
to impossible.

Alkalinity and pH
Alkalinity and pH are two important factors in bio-gas production. pH is used to indicate
whether a solution is acidic or basic. An acidic solution has more hydrogen or hydronium ions
than hydroxide ions. A basic solution has more hydroxide than hydronium ions. At a pH of 7
there are equal amounts. A pH greater than 7 is classified as a basic solution and a pH less than
7 is classified as an acidic solution.
Alkalinity is the measure of the amount of carbonate in a solution. Alkalinity is important
because as an acid is added to a solution, carbonates will contribute hydroxide ions, which tend
to neutralize the acid, thus affecting the pH of a solution. This is known as the buffering effect
of alkalinity.
pH is important to bio-gas production because the bacteria responsible for bio-gas production
flourish within a narrow range of pH, between 6.5 and 8.0. As the acid-forming bacteria
produces acid, the methane-forming bacteria use the acid and aid in maintaining a neutral pH in
the unit. It is important to know that the reaction rate for the acid-forming bacteria is much
faster than the reaction rate involving the methane-forming bacteria. Therefore it is important
that a good balance is maintained between the two in order to avoid acidification of the manure.
This means that you need to maintain and nurture a higher population of methane-forming
bacteria.
In order to facilitate a large population of methane-forming bacteria you can raise the pH to
about 7.5 by adding baking soda. The baking soda increases the alkalinity or buffering capacity
of the unit. This may or may not be necessary to do. If you are not producing bio-gas test the
pH of the unit, and if you have an acidic solution you know to add baking soda the next time you
mix the manure and water together.
When the initial reactions occur in the unit there is a large production of acid-forming
bacteria. The methane-forming bacteria population may not be large enough to maintain a
neutral pH in the unit. If the unit drops below 6.5 the methane-forming bacteria will begin to
die, pushing the bacteria populations further out of balance. This results in an acidic solution
within the unit, which can not produce bio-gas.

Formation of Foam
Some times the unit will form a cap of foam on the top of the manure mixture inside of the
barrel. This can diminish the production of bio-gas and stop the flow of bio-gas to the inner
tubes. If the organic matter and water was mixed well before introduction to the unit this
problem can usually be prevented. It is also less likely to occur if the organic material you are
using is 100% manure and has no plant matter mixed with it. To prevent the formation of foam
in the unit it is a good idea to agitate the unit twice weekly as mentioned before.

Burning Bio-gas
Bio-gas can be used with almost any small stove. The simpler the better, meaning you may
want to construct your own. You can do this simply by using plastic tubing and a simple metal
frame.
Or you can use the small stoves available here in Bolivia. Which ever type of stove you use,
make sure that there is not too much air mixing with the gas before it is lit. If you are producing
a small amount of methane and you mix the bio-gas with too much outside air, it may not ignite.
The opposite is true as well; if you have too much bio-gas and not enough outside air the gas
may not ignite.

So make sure that you can adjust the air mix on the stove, it may take some experimentation
to get the bio-gas to light. When the bio-gas/air mix is right the gas will ignite and a blue flame
will result, try to obtain as blue of a flame as you can by adjusting the mixture.
Over time your stove may become clogged, or the flame may turn from blue to yellow. This
means that your line is dirty and that you need to clean the stove. You can clean the stove with a
brush, warm water, and soap. Make sure the stove is completely dry before reassembling it and
using it to cook with once again.

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