Description

Organism: Homo sapiens, human

Tissue: HeLa contaminant
Product Sheet Disease: Carcinoma
Morphology: epithelial
HEp­2 (ATCC® CCL­23™) Hela Markers: Y
Growth Properties: adherent
Virus Susceptibility:
Please read this FIRST Viral Testing: ATCC confirmed this cell line is positive for the presence of human papilloma viral DNA
sequences via PCR.
Isoenzymes:
Storage Temp.
G6PD, A
liquid nitrogen DNA Profile:
vapor phase Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
Biosafety Level D5S818: 11,12
2 D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Intended Use Cytogenetic Analysis: Occasional polyploids. Several marker chromosomes were observed along with
frequent minutes, and often 2 large chromosomes with subterminal [Link] Marker Chromosomes:
This product is intended for research use only. It is not
One copy of M2, two­four copies of M3 and one copy of M4 as revealed by G­banding patterns.
intended for any animal or human therapeutic or
diagnostic use.
SAFETY PRECAUTION

Complete Growth Medium

ATCC highly recommends that protective gloves and clothing always be used and a full face mask always be
worn when handling frozen vials. It is important to note that some vials leak when submersed in liquid nitrogen
The base medium for this cell line is ATCC­formulated
and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas
Eagle's Minimum Essential Medium, Catalog No. 30­
phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris.
2003. To make the complete growth medium, add the
following components to the base medium: fetal bovine
serum to a final concentration of 10%. Unpacking & Storage Instructions

Citation of Strain 1. Check all containers for leakage or breakage.

2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature
If use of this culture results in a scientific publication, it below ­130°C, preferably in liquid nitrogen vapor, until ready for use.
should be cited in that manuscript in the following
manner: HEp­2 (ATCC® CCL­23™) Handling Procedure for Frozen Cells

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If
upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor
phase and not at ­70°C. Storage at ­70°C will result in loss of viability.
1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep
the O­ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by
dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out
under strict aseptic conditions.
3. It is recommended that the cryoprotective agent be removed immediately. Centrifuge the cell
suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the
cell pellet in an appropriate amount of fresh growth medium.
4. Transfer the vial contents to an appropriate size vessel. It is important to avoid excessive alkalinity of
the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents,
the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to
American Type Culture Collection
allow the medium to reach its normal pH (7.0 to 7.6).
PO Box 1549
Manassas, VA 20108 USA 5. Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if
[Link] using the medium described on this product sheet.

800.638.6597 or 703.365.2700
Fax: 703.365.2750 Handling Procedure for Flask Cultures
Email: Tech@[Link]
The flask was seeded with cells (see specific batch information) grown and completely filled with medium at
Or contact your local distributor
ATCC to prevent loss of cells during shipping.

Page 1 of 3
 1. Upon receipt visually examine the culture for macroscopic evidence of any microbial contamination.
Using an inverted microscope (preferably equipped with phase­contrast optics), carefully check for
any evidence of microbial contamination. Also check to determine if the majority of cells are still
attached to the bottom of the flask; during shipping the cultures are sometimes handled roughly and
many of the cells often detach and become suspended in the culture medium (but are still viable).
2. If the cells are still attached, aseptically remove all but 5 to 10 mL of the shipping medium. The
Product Sheet
shipping medium can be saved for reuse. Incubate the cells at 37°C in a 5% CO2 in air atmosphere
HEp­2 (ATCC® CCL­23™) until they are ready to be subcultured.
3. If the cells are not attached, aseptically remove the entire contents of the flask and centrifuge at
125 x g for 5 to 10 minutes. Remove shipping medium and save. Resuspend the pelleted cells in 10
mL of this medium and add to 25 cm2 flask. Incubate at 37°C in a 5% CO2 in air atmosphere until cells
Please read this FIRST
are ready to be subcultured.

Storage Temp.
Subculturing Procedure
liquid nitrogen
vapor phase
Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an
additional 1 to 2 mL of trypsin­EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the
cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Biosafety Level
Medium Renewal: 2 to 3 times per week
2

Cryopreservation Medium

Intended Use Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is
available as ATCC Catalog No. 4­X.
This product is intended for research use only. It is not
intended for any animal or human therapeutic or Comments
diagnostic use.
Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was
Complete Growth Medium originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found,
based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established
The base medium for this cell line is ATCC­formulated via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. ATCC confirmed
Eagle's Minimum Essential Medium, Catalog No. 30­ this cell line is positive for the presence of human papilloma viral DNA sequences via PCR.
2003. To make the complete growth medium, add the
following components to the base medium: fetal bovine References
serum to a final concentration of 10%.

References and other information relating to this product are available online at [Link].
Citation of Strain
Biosafety Level: 2
If use of this culture results in a scientific publication, it
should be cited in that manuscript in the following
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in
manner: HEp­2 (ATCC® CCL­23™)
the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S.
Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes
for Health.

ATCC Warranty

The viability of ATCC® products is warranted for 30 days from the date of shipment, and is valid only if the
product is stored and cultured according to the information included on this product information sheet. ATCC
lists the media formulation that has been found to be effective for this strain. While other, unspecified media
may also produce satisfactory results, a change in media or the absence of an additive from the ATCC
recommended media may affect recovery, growth and/or function of this strain. If an alternative medium
formulation is used, the ATCC warranty for viability is no longer valid.

Disclaimers

American Type Culture Collection

PO Box 1549
This product is intended for laboratory research purposes only. It is not intended for use in humans.
Manassas, VA 20108 USA While ATCC uses reasonable efforts to include accurate and up­to­date information on this product sheet,
[Link] ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and
patents are provided for informational purposes only. ATCC does not warrant that such information has been
800.638.6597 or 703.365.2700
confirmed to be accurate.
Fax: 703.365.2750
Email: Tech@[Link]
This product is sent with the condition that you are responsible for its safe storage, handling, and use. ATCC
is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort
Or contact your local distributor is made to insure authenticity and reliability of strains on deposit, ATCC is not liable for damages arising from
the misidentification or misrepresentation of cultures.
Page 2 of 3
 Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this
product. The MTA is also available on our Web site at [Link]

Additional information on this culture is available on the ATCC web site at [Link].
© ATCC 2013. All rights reserved. ATCC is a registered trademark of the American Type Culture Collection. [05/31]

Product Sheet
HEp­2 (ATCC® CCL­23™)

Please read this FIRST

Storage Temp.
liquid nitrogen
vapor phase

Biosafety Level
2

Intended Use

This product is intended for research use only. It is not

intended for any animal or human therapeutic or
diagnostic use.

Complete Growth Medium

The base medium for this cell line is ATCC­formulated

Eagle's Minimum Essential Medium, Catalog No. 30­
2003. To make the complete growth medium, add the
following components to the base medium: fetal bovine
serum to a final concentration of 10%.

Citation of Strain

If use of this culture results in a scientific publication, it

should be cited in that manuscript in the following
manner: HEp­2 (ATCC® CCL­23™)

American Type Culture Collection

PO Box 1549
Manassas, VA 20108 USA
[Link]

800.638.6597 or 703.365.2700
Fax: 703.365.2750
Email: Tech@[Link]

Or contact your local distributor

Page 3 of 3