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Answered paper 6 3/9

Cambridge International Examinations
Cambridge International General Certificate of Secondary Education
* 2 5 1 5 0 4 6 5 3 2 *

BIOLOGY 0610/63
Paper 6 Alternative to Practical October/November 2018

Answered
1 hour
Candidates answer on the Question Paper.
No Additional Materials are required.

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use an HB pencil for any diagrams or graphs.
Do not use staples, paper clips, glue or correction fluid.
DO NOT WRITE IN ANY BARCODES.

Answer all questions.

Electronic calculators may be used.

You may lose marks if you do not show your working or if you do not use appropriate units.

At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

This syllabus is approved for use in England, Wales and Northern Ireland as a Cambridge International Level 1/Level 2 Certificate.

This document consists of 10 printed pages and 2 blank pages.

DC (ST/JG) 152887/5
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1 Catalase is an enzyme found in plant and animal cells. It catalyses the break down of hydrogen
peroxide to form water and oxygen.

2H2O2 2H2O + O2
hydrogen peroxide water oxygen

The oxygen produced during the reaction forms a foam on the surface of the mixture of hydrogen
peroxide and catalase. The height of the foam can be used as a measure of the activity of the
catalase present. Dependent
Independent
A student investigated the effect of cooking on the activity of catalase in potato tissue using 3%
hydrogen peroxide. Outcome Constant variables
1. Temp
The student used the following method: 2. PH
c. U 3. Volume of substrate and enzyme
Step 1 Cut two potato sticks so that they are identical in both shape and size.
c.u
Step 2 Put 5 cm3 of hydrogen peroxide solution into a test-tube labelled uncooked potato.

Step 3 Put 5 cm3 of hydrogen peroxide solution into a test-tube labelled cooked potato.

Step 4 Put one of the potato sticks into a beaker of hot water (90 °C) and leave it for five minutes
before carefully removing it. Source of error …starting the exp with different temperatures
Without drying
Step 5 Put the potato stick from step 4 into the test-tube labelled cooked potato.

Step 6 Put the uncooked potato stick into the test-tube labelled uncooked potato.
c. u
Step 7 Leave the potato sticks in the hydrogen peroxide for three minutes and then measure
the height of the foam produced in each of the test-tubes.

Dependent:- recorded/ measured / counted, observed.

Indepen'dent: changable/ effect of-

Dr. Mody Is crazy.

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1 Catalase is an enzyme found in plant and animal cells. It catalyses the break down of hydrogen
peroxide to form water and oxygen.
substrate outcomejm
t controlled U .
:
2H2O2 2H2O + O2
hydrogen peroxide water oxygen emp .
substrate
•
PH cone .

The oxygen produced during the reaction forms a foam on the surface of the mixture of hydrogenEnzymecone
•

peroxide and catalase. The height of the foam can be used as a measure of the activity of theoutcome
catalase present. Dependent
indep .
C. U
A student investigated the effect of cooking on the activity of catalase in potato tissue using 3%
I

hydrogen peroxide.

The student used the following method:

C- U
Step 1 Cut two potato sticks so that they are identical in both shape and size.

Step 2 Put 5 cm3 of hydrogen peroxide solution into a test-tube labelled uncooked potato.
-

Step 3 Put 5 cm3 of hydrogen peroxide solution into a test-tube labelled cooked potato. indep .

Step 4 Put one of the potato sticks into a beaker of hot water (90 °C) and leave it for five minutes
before carefully removing it. Without dying / diff
starting
Step 5 Put the potato stick from step 4 into the test-tube labelled cooked potato.
temp .

Step 6 Put the uncooked potato stick into the test-tube labelled uncooked potato.
c.u
Step 7 Leave the potato sticks in the hydrogen peroxide for three minutes and then measure
the height of the foam produced in each of the test-tubes.

↳DÉ
No repeats .

Dependent:- recorded/ measured / counted, observed.

Indepen'dent: changable/ effect of-

Dr. Mody Is crazy.

Conclusion ; independent + outcome

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Fig. 1.1 shows the test-tubes after three minutes.

uncooked 13 cooked
potato potato
v8 12
11 .
measure
11 theheight of

3- 2
/ foam
of 10
at
Foam
☐ From
→

same
9
at
=
00
starting point
8
2) Use same
7
ruler .

hydrogen 6 37 keep the

-

peroxide
5 test tube at
the same distance
4
From the ruler .

3 4) take
test-tube
.

reading
2 From ruler at same

1 angle to avoid
cm parallax
error .

desk ruler
potato stick

Fig. 1.1

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 5

(a) (i) Prepare a table for the student’s results.

Measure the height of the foam in each of the test-tubes in Fig. 1.1. Record these values
in your table.

Treated potato pieces Height of foam / cm

Cooked 1.0

Uncooked
3.2

[3]

(ii) Calculate the difference in the height of the foam produced by cooked and uncooked
potato after three minutes. Include the units.

Space for working.

3.2-1.0=2.2 cm

2.2
............................................................[1]

(iii) Describe how you made sure that your measurements of the height of the foam in each
test-tube could be compared.

Use the same ruler

...........................................................................................................................................

Measure from the same Starting point.

...........................................................................................................................................

.......................................................................................................................................[1]

(iv) State a conclusion for these [Link] of independent on outcome

Uncooked had higher enzyme activity
...........................................................................................................................................

Or Uncooking showed a longer foam height

...........................................................................................................................................

.......................................................................................................................................[1]

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(b) (i) Identify the variable that was changed (independent variable) in this investigation.

treatment of potato being cooked / un

.......................................................................................................................................[1]
cooked .

(ii) State three variables, other than the way in which you measured the height of the foam,
that should have been kept constant in this investigation.

volume of Her / correct Hzoz

1 ........................................................................................................................................
.

Size of Potato pieces / species

2 ........................................................................................................................................
.
.

Same starting temp

3 ........................................................................................................................................
.

PH Sufaceareaof Potato [3]

(c) Identify three sources of error in this method. pieces .

no repeats
1 ................................................................................................................................................
.

...................................................................................................................................................

not starting with same temp as the cooked

2 ................................................................................................................................................
.

potato was not cooled before adding 1-011202

................................................................................................................................................... .

height of foam is uneven so inaccurate

way
3 ................................................................................................................................................
of measurement
...................................................................................................................................................
[3]
6. Didnt dry potato Piece before adding to
112oz may cause dilution of Koz .

affecting results .

5. Potato
pieces used may not bed
same type / age / species .

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outcome Same age

Inder ? activity of catalase
→

oWIpis¥F size / mass

{
.

7
Same volume

{
→
(d) A student was asked to test the hypothesis:
' of Hzoz + Time
nd .

Catalase activity is the same in all species of plants. →

Temp / PH . .

Dep → .

Plan an investigation to test this hypothesis.

Ind .
get 5 different plant types / species
...................................................................................................................................................
.
.

cont v. all should be cut to same size

...................................................................................................................................................
}
Use 5 test tubes with same volume of Hzoz 5cm
...................................................................................................................................................

and same concentration , control Temp

Using W Band
...................................................................................................................................................
.

same PH
Using Buffer Solution
...................................................................................................................................................

Time interval leave them soaked in Hroz for homing

...................................................................................................................................................
. .

Safety : eye goggles

...................................................................................................................................................
.

Control expi: Used Boiled Plant / No plant

...................................................................................................................................................
- .

Dependent : measure the volume of oxygen gas produced

...................................................................................................................................................

using a
gas syringe
...................................................................................................................................................
.

Repeat using more plant pieces

of each species
...................................................................................................................................................
→

to take average and reduce error

...................................................................................................................................................
.

...............................................................................................................................................[6]

(e) Potatoes contain a lot of starch. When potatoes start to grow to form new plants, the starch is
broken down by the enzyme amylase to form reducing sugars.

Explain how you could test samples of potato to show that starch and reducing sugars are
present.

Steps of planning an investigation

starch ........................................................................................................................................

Independent
...................................................................................................................................................

Controlled variable + methodology ( w.B

................................................................................................................................................... , buff
equilibration)
reducing sugars ........................................................................................................................

Time interval
...................................................................................................................................................

Dependent
...................................................................................................................................................

Safety
...................................................................................................................................................
[5]
Control experiment
Repeat ( using larger sample) replicates
[Total: 24]

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ndep
outc%f
'

(d) A student was asked to test the hypothesis:

+
Catalase activity is the same in all species of plants.

Methodology
team
.

Plan an investigation to test this hypothesis. same +

Independent ...................................................................................................................................................
Get 5 different plant species
Controlled variables + methodology
...................................................................................................................................................
All should be of same size
Time interval...................................................................................................................................................
Us4 5 test tubes with the same volume of H2O2
Dependent
Same pH using buffer solution
...................................................................................................................................................

Safety Same temperature using thermostatic water bath

...................................................................................................................................................

Control ...................................................................................................................................................

Repeat Leave them soaked in H2O2 for 10 mins

...................................................................................................................................................

...................................................................................................................................................
Measure the volume gas produced using gas syringe
...................................................................................................................................................

Wear eye goggles

...................................................................................................................................................

...................................................................................................................................................
Repeat using boiled plants
...................................................................................................................................................

Repeat : using 3 more plant pieces of each species to calculate the mean value
...............................................................................................................................................[6]

(e) Potatoes contain a lot of starch. When potatoes start to grow to form new plants, the starch is
broken down by the enzyme amylase to form reducing sugars.

Explain how you could test samples of potato to show that starch and reducing sugars are
present.

starch ........................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

reducing sugars ........................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................
[5]

[Total: 24]

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2 Woodlice are small animals.

The rate of respiration of a woodlouse can be measured using a simple respirometer as shown in
Fig. 2.1.

As the woodlouse respires the drop of coloured liquid moves along the capillary tube.

ruler
Start

mesh barrier cm
1 2 3 4 5 6

capillary drop of
potassium woodlouse tube coloured liquid
hydroxide to absorb airtight
carbon dioxide bung

ruler
After 30 minutes

mesh barrier cm
1 2 3 4 5 6

drop of capillary
potassium woodlouse coloured liquid tube
hydroxide to absorb airtight
carbon dioxide bung

Fig. 2.1

(a) (i) Record the position of the drop of coloured liquid in the capillary tube shown in Fig. 2.1 at
the start and after 30 minutes.

45
Start ........................................................ mm

5
After 30 minutes ........................................................ mm
[1]

(ii) Using the information in 2(a)(i), calculate the rate of movement of the drop of coloured
liquid in mm per minute. Give your answer to one decimal place.

Space for working. 45€

30
=

4¥ = 1.3

13_
.................................. mm per minute [2]
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Dependent
(b) The rate of movement of the drop of coloured liquid along the respirometer can be used
as a measure of the rate of respiration. A student used the apparatus shown in Fig. 2.1 to
investigate the rate of respiration in different species of small animals.

The results are shown in Table 2.1.

Table 2.1
non - measurable .

rate of movement of drop of coloured liquid / mm per minute

animal species
repeat 1 repeat 2 repeat 3 average
A 1.6 1.7 1.3 1.5

±
B 0.9 1.0 0.7 0.9
C 2.4 2.6 2.5 2.5
D 1.9 2.0 1.9 1.9

(i) Plot a bar chart on the grid to show

-
the average rate of movement of the drop of coloured
liquid in the capillary tube for the four different species of animal.

2. 5

15

I
-

0.5

A B C D
[3]

(ii) State which letter represents the animal species with the highest rate of respiration.
C
.......................................................................................................................................[1]
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(iii) The student decided it would be better to calculate the rate of respiration per gram of
animal so that the values could be compared.

Describe how the student could find out the rate of respiration per gram of animal.
Measure the mass of the animal
...........................................................................................................................................

Then divide rate of respiration by mass

...........................................................................................................................................

...........................................................................................................................................

.......................................................................................................................................[2]

(c) Fig. 2.2 shows a photograph of a woodlouse.

i* i.
Q

Fig. 2.2

(i) Draw a large diagram of the woodlouse in Fig. 2.2.

*
[4]
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(ii) The magnification of the woodlouse in Fig. 2.2 is ×9.

Measure the width of the woodlouse along line PQ. Include the unit.

length of line PQ ......................................................

Calculate the actual width of the woodlouse using the formula. Include the unit.

length of line PQ on Fig. 2.2

magnification =
actual width of woodlouse

Show your working and give your answer to two decimal places.

................................................................
[3]

[Total: 16]

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