Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
1. Statement 1: Asexual Reproduction provides opportunities for
variations and formulation of unique combinations of genetic set-up
Statement 2: In a chromosome, a specific DNA sequence responsible for
initiating replication is called Initiator codon.
A. Both Statement 1 and 2 are Correct
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
2. The first restriction endonuclease was
A. Hind II
B. Hind III
C. EcoR I
D. pBR 322
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
3. The cutting of DNA at specific locations became possible with the
discovery of….
A. Molecular glues
B. Molecular scissors
C. Plasmids
D. Vectors
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
4. Statement 1: The Green Revolution succeeded in tripling the food
supply. Statement 2: The capacity to generate a whole plant from any
cell/ explant is called Totipotency.
A. Both Statement 1 and 2 are Correct
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
5. Which of the following is free of virus –
A. Apical meristem
B. Axillary meristem
C. Both a and b
D. None of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
6. Assertion: DNA always moves towards anode during gel-electrophoresis.
Reason: DNA consist of a phosphate backbone which is a positively charged,
hence when the DNA is placed in gel- electrophoresis it always moves
towards anode, as the anode is negatively charged.
A. Both As. And Rs. Are True and Rs. Is Correct Explanation of As.
B. Both As. And Rs. Are True but Reason is not Correct Explanation of As.
C. Assertion is True but Reason is False
D. Both Assertion and Reason are False
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
7. Restriction enzymes belong to which class of enzymes –
A. Hydrolases
B. Ligases
C. Isomerases
D. Nucleases
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
8. Mark the odd one out with respect to the group to which the
following belongs –
A. Armyworm
B. Tobacco budworm
C. Hookworm
D. None of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
9. Which of the following is used for staining to visualise DNA in Gel
electrophoresis –
A. NaOH
B. HCL
C. Ethidium bromide
D. Agarose
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
10. Which of the following features are required to facilitate cloning into
a vector?
A. Origin of Replication
B. Selectable marker
C. Cloning site
D. All of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
11. Which of the following Enzyme is used for isolating genetic material
from plant cells-
A. Cellulase
B. Chitinase
C. Lysozyme
D. Ribozyme
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
12. Statement 1: The first clinical gene therapy was given in 1880 to a 4
year old girl.
Statement 2: The first clinical gene therapy was performed for treating
Adenosine deaminase deficiency.
A. Both Statement 1 and 2 are Correct
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
13. Agrobacterium tumifaciens is pathogenic to :
A. several monocot plants
B. several dicot plants
C. Bryophytes
D. Gymnosperms
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
14. Bt toxin gets activated at which pH
A. Alkaline
B. Acidic
C. Neutral
D. Both b and c
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
15. The proteins encoded by the genes crylAc control….
A. Cotton bollworms
B. Corn borer
C. Both a and b
D. None of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
16. The volume of Bioreactors cannot be
A. 20 L
B. 120 L
C. 170 L
D. 220 L
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
17. Stirred-tank bioreactors are defined for-
A. Availability of 02 throughout the process
B. Purification of the product
C. Addition of preservative
D. Seperation of DNA fragments
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
18. Which of the following technique is / are used for Early diagnosis of
diseases?
A. Recombinant DNA technology
B. Polymerase Chain Reaction (PCR)
C. Enzyme Linked Immuno-sorbent Assay
D. All of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
19. Downstream processing includes
A. Seperation and Distillation
B. Expression and Seperation
C. Selection and Purification
D. Seperation and Purification
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
20. Which of the following is not a tool for Genetic engineering?
A. Restriction endonuclease
B. Transacetylase
C. DNA Ligase
D. Plasmid
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
21. Insulin used for Diabetes was earlier extracted from and Pigs – of
slaughtered cattle
A. Pancreas
B. Liver
C. Kidney
D. Small Intestine
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
22. At present, about how many recombinant therapeutics have been
approved for human-use all over the world –
A. 30
B. 13
C. 12
D. 300
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
23. The two polypeptide chains of human insulin are linked together by-
A. Hydrogen bonds
B. Phosphodiester linkage
C. Covalent bond
D. Disulphide bridges
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
24. Eli Lilly company synthesised
A. Human glucagon
B. Human Insulin
C. Somatostatin
D. Pancreatic polypeptide
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
25. ADA deficiency is caused due to :
A. Substitution
B. Translocation
C. Inversion
D. Deletion
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
26. A probe is a …….stranded sequence of DNA or RNA.
A. Single
B. Double
C. Single or Double
D. None of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
27. Which of the following technique is based on the principle of
Antigen- Antibody interaction:
A. PCR
B. Recombinant DNA technology
C. ELISA
D. All of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
28. Statement 1: 75% of all transgenic animals are mice.
Statement 2: Rosie produced human protein- enriched milk (1.4 grams
per litre)
A. Both Statement 1 and 2 are Incorrect
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
29. How many varieties of rice have been estimated to be present in
India?
A. 2,000
B. 200
C. 20,000
D. 200,000
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
30. How many documented varieties of Basmati rice are grown in India?
A. 2,000
B. 27
C. 127
D. 50,000
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
31. Which of the following term is used to refer to the use of bio-
resources by multinational companies & other organisations without
proper authorisation from the countries and people concerned without
compensatory payment-
A. Bioremediation
B. Biometry
C. Privacy
D. Biopiracy
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
32. The sequence that controls the copy number of the linked DNA in
the vector, is termed as –
A. ori site
B. Palindromic sequence
C. Recognition site
D. Selectable marker
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
33. GEAC stands for
A. Genetic Engineering Approval Committee
B. Genetic and Environment Approval Committee
C. Genetics Engineering Appraisal Committee
D. Genome Engineering Approval Committee
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
34. The vector requires a gene whose expression helps in identifying and
eliminating non-transformants. This gene is known as:
A. Origin of replication
B. Structural gene
C. Plasmid
D. Selectable marker
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
35. The word ‘restriction’ in restriction enzyme refers to-
A. Cutting of DNA at specific position only
B. Cleaving of phosphodiester linkage
C. Prevention of the multiplication of bacteriophage in bacteria
D. All of the above
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
36. Which of the following is being used to precipitate DNA from the
suspension obtained from the lysis of cell?
A. Divalent cations like calcium
B. Chilled ethanol
C. Ethidium bromide
D. Antibiotics like Chloramphenicol
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
37. Which part of the tobacco plant is infected by Meloidogyne
incognita?
A. Root
B. Leaf
C. Stem
D. Flower
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
38. RNA interference is used for which of the following purposes in the
field of biotechnology?
A. To develop a pest resistant plant against infestation by nematode
B. To enhance the mineral usage by the plant
C. To reduce post harvest losses
D. To develop a plant tolerant to abiotic stresses
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
39. Bt cotton variety that was developed by the introduction of toxin
gene of Bacillus thuringiensis (Bt) is resistant to-
A. Fungal diseases
B. Plant nematodes
C. Insect predators
D. Insect pests
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
40. Plasmids are used as vectors in genetic engineering because of their:
A. Resistance to restriction enzymes
B. Ability to cause infection in host
C. Resistance to antibiotics
D. Ability to carry foreign genes
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
41. In pBR322, tetracycline resistance gene (tet) has recognition site for
which of the following restriction endonuclease?
A. Hind III
B. Bam HI
C. EcoRI
D. Pst I
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
42. Genetic engineering would not have been possible if one of these
were absent-
A. DNA polymerase
B. Reverse transcriptase
C. DNA ligase
D. RNA synthetase
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
43. The sticky ends generated by action of EcoRI on insert DNA facilitates
action of which enzyme?
A. DNA polymerase
B. Taq polymerase
C. Alkaline phosphotase
D. DNA ligase
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
44. Organisms which can be used to gain commercial benefits are called-
A. Beneficial resources
B. Bioresources
C. Financial resources
D. Biodegradable resources
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
45. The Taq polymerase enzyme is obtained from-
A. Thermus aquaticus
B. Thiobacillus ferroxidants
C. Bacillus subtilis
D. Pseudomonas putida
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
46. The correct order of steps in PCR is-
A. Denaturation, Extension, Annealing
B. Purification, Annealing, Extension
C. Denaturation, Annealing, Extension
D. Extension, Annealing, Denaturation
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
47. Statement 1: Human Insulin can be produced by protozoa. Statement
2: Insulin from different animals cause allergic reactions in humans.
A. Both Statement 1 and 2 are Correct
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
48. Agarose extracted from the sea- weeds is used in-
A. PCR amplification
B. Electroporation
C. Gel electrophoresis
D. Cybrid production
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
49. Silencing of mRNA has been used in producing transgenic plants
resistant to-
A. bollworms
B. white rusts
C. nematodes
D. bacterial blights
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
50. Statement 1: Biotechnology deals with techniques of using live organisms or
enzymes from organisms to produce products and processes useful to humans.
Statement 2: Today more than 2300 restriction enzymes have been isolated from
over 900 strains of bacteria.
A. Both Statement 1 and 2 are Correct
B. Both Statement 1 and 2 are Incorrect
C. Statement 1 is Correct
D. Statement 2 is Correct
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
51. Bt toxin protein crystals present in bacterium Bacillus thuringiensis,
do not kill the bacteria themselves because-
A. Bacteria are resistant to the toxin
B. Toxins occur as inactive protoxins in bacteria
C. Bacteria enclose toxins in a special sac
D. None of these
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
52. In gel electrophoresis, differential mobility of DNA depends upon-
A. Helical nature of DNA
B. Double stranded nature of DNA
C. Size of DNA
D. Hydrogen bonding between bases
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
53. Which of the following statements does not hold true for restriction
enzyme?
A. It recognises a palindromic nucleotide sequence.
B. It is an endonuclease.
C. It is isolated from bacteria.
D. It produces the different kind of sticky ends in different DNA
molecules.
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
54. Pomato is a result of :
A. Embryo culture
B. Micro-propagation
C. Tissue culture
D. Somatic hybridisation
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
55. At what temperature heat shock treatment is given to make bacterial
cells competent?
A. 42° F
B. 104° C
C. 42° C
D. 24° C
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
Answers
1)B 13)B 29)D
14)A 30)B
2)A
15)A 31)D
3)B 16)A 32)A
4)A 17)A 33)A
18)D 34)D
5)C
19)D 35)C
6)C 20)B 36)B
7)D 21)A 37)A
8)C 22)A 38)A
23)D 39)D
9)C
24)B 40)D
10)D 25)D 41)B
11)A 26)A 42)C
27)C 43)D
12)D
28)B 44)B
45)A
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Biology at Ease
Community Post 49: Biotechnology Unit NEET Biology
Answers
46)C
47)D
48)C
49)C
50)C
51)B
52)C
53)D
54)D
55)C
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