SAS Super 100/180 & Duo 360 Manual
SAS Super 100/180 & Duo 360 Manual
Patent Pending
Manufactured by International pbi Spa Milan, Italy
REFERENCES
FDA – 1987 Guideline on Sterile Drug Products produced by Aseptic Process
ACGIH – Guideline for the Assessment of Bioaerosol in the Indoor Environment
ASTM – Draft Protocol – Committee D22.05.06
USP 23-NF 18 8th Supplement 1116 (May 1998) – Microbiological Evaluation of Clean Rooms and other Controlled
Environments
EU Guide for GMP – Manufacture of Sterile Medicinal Products Control of Medicines and Inspection
CEN/TC 243 Norms for Clean Room Technology
International PBI S.p.A. Via Novara, 89 – 20153 Milano – Italy – Tel + 39 02 48779-1 – Fax + 39 02 40090010
E-mail: info@[Link] – [Link]
International pbi S.p.A. « Sas Super 100/180, Duo Sas 360, Sas Isolator » October 2006 Rev. 5 1
INSTRUCTIONS MANUAL
SAFETY INSTRUCTIONS
• Use the device just for the indicated purposes.
• The Device must be correctly used according to manual instructions,before starting any operation.
• Replace immediately any electrical cable when damaged ; never use a damaged or weared electrical cable.
• Always disconnect the charger before:
A. Reparing or maintenance; these operations must be carried out by qualified staff;
B. Keep the unit clean;
• Use original spare parts and accessories for any replacement;
GENERAL CAUTION
Be aware that the voltage and frequence of the electrical system are compatible with
the electrical requirements of the battery charger.
CAUTION
CAUTION
International pbi S.p.A. « Sas Super 100/180, Duo Sas 360, Sas Isolator » October 2006 Rev. 5 2
SUMMARY
SECTION ONE PAGE
Brief Operating Instructions 5
Visual Display Presentation 5
SECTION TWO
Operating Instructions 7
1. Principle 7
2. The basic idea (Sas Isolator / Sas Super100-180 / Duo-Sas 360) 7
3. Installation 8
3.1 The Practical Use 8
3.2. List of MENU and SUB-MENU 9
3.3. Preliminary Inspection 10
3.4. 55 mm Contact Plate holder adjusting 12
3.5. 84 mm Maxi Contact Plate use (optional) 12
3.6. Tripod Installation (optional) 12
4. Operations 12
4.1. Routine Sampling (Sas Super100-180 / Sas Isolator) 13
4.1.A. Routine Sampling (Duo Sas 360) 13
4.2. Standard Mode Sampling (Sas Super100-180 / Sas Isolator) 13
4.2.A. Standard Mode Sampling (Duo Sas 360) 14
4.3. Utility Mode Program 15
4.3.1. Set Time 15
4.3.2. Sampling Site 15
4.3.3. Operator Identification 16
4.3.4. Language 16
4.3.5. Clear Record 17
4.3.6. Display Record 17
4.3.7. Print 17
4.3.8. Autoswitch OFF 18
4.3.9. Mode 18
4.4. User Mode 19
4.5. Program Mode 19
4.6. Delay Mode 20
4.7. Multi Mode 21
4.8. Low Battery Message 23
4.9. Remote Switch Use 23
4.10. Contact Plate Holder Adjustment 24
4.11. Sanitation of Air Sampler 24
4.12. Contact Plate 25
4.13. Use In Clean Room 25
4.14. Contact Plate Preparation 26
4.15. Total Bacterial Count and/or Micro-organisms Identification by Dilution 26
4.16. Multi Point Sampling with several independent air samplers 26
4.17. Multi Point Sampling with several connected air samplers 26
5. Final Operation 27
6. Result Calculation and Recording 27
6.1. CFU/1000 Litres 27
6.2. Data Recording 28
« Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 3
SECTION THREE
7. Specifications 29
7.1. Messages Shown On Display 31
7.2. Sampling Time Requested For Different Air Volumes 31
7.3. Battery Charger Operation 31
7.4. Automatic Switch OFF 32
7.5. Sampling at High Altitude Over The Sea Level 32
SECTION FOUR
8. Maintenance 33
8.1. Cleaning 33
8.2. Validation and Calibration 33
8.3. Performance Routine Monitoring 33
8.4. Trouble Shooting 33
8.5. Battery Replacement 33
8.6. Accessory and Spare Part List 34
SECTION FIVE
9. Brief Instructions 36
SECTION SIX
10. EN 17025 Documentation 44
SECTION SEVEN
11. SAS SUPER ISOLATOR SCHEMATIC 45
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 4
SECTION ONE
• BRIEF OPERATING INSTRUCTIONS FOR SAS SUPER 100 / 180,
The air flow is visualized on digital display at the fourth automatic succession presentation each time the
instrument is switched on.
Press ON/OFF switch.
Press START button to sample the same air volume as the last sampling cycle.
To change the volume of air, use arrows to select “Standard Mode” from menu.
Refer to instruction manual to change settings.
Press ENTER to confirm selection.
Press arrows for menu selection to modify other pre-set parameters:
STANDARD MODE
USER MODE
PROGRAM MODE
DELAY MODE
MULTI MODE
UTILITY MODE
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 5
DUO SAS SUPER 360
This information appears in automatic succession. Avoid pushing any button before the end of the few seconds
presentation; during this presentation all functions are disconnected.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 6
SECTION TWO
• OPERATING INSTRUCTIONS
SAS ISOLATOR
Critical air monitoring in isolator and barrier environments
It consists of a programmable unit which remains outside the
controlled area and an independent sampling head which uses
RODAC Contact Plates or Standard 90 mm Petri dishes. See Section
seven page 43.
The two air samplers have the same performances with the only difference being in the rate of air aspirated:
SAS SUPER 100 = 100 litres of air per minute
SAS SUPER 180 = 180 litres of air per minute.
The SAS SUPER 180 is appropriate in Clean Rooms and other applications demanding fast sampling times. In Clean
Rooms, for example, it is important to test a larger volumes of air, because the microbial air contamination is very low.
The SAS SUPER 180 reduces the required time to obtain a sample with an important saving of time.
Both SAS SUPER 100 and SAS SUPER 180 are identified by the “SAS SUPER 100” logo on the right side of the
instrument.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 7
The SAS SUPER 180 air sampler is recognisable by the SAS SUPER 180 logo that appears on the display when the
operator turns on the unit.
3. INSTALLATION
3.1 The practical use of contact plates
SAS SUPER 100 SAS SUPER 180 DUO SAS SUPER 360 & SAS ISOLATOR FLOW SCHEME
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 8
3.2. LIST OF MENUS AND UTILITY SUB-MENUS
MENU DESCRIPTION
START FOR XXX This message indicates the air sampler is ready to repeat the same volume of
prior sampling
STANDARD MODE This message indicates the air sampler can be set to select one of 8 fixed
standard sampling volumes
USER MODE This message indicates the air sampler can be set to select one of 8
programmable sampling volumes
PROGRAM MODE This message indicates the air sampler can be set to modify the 8
programmable sampling volumes
DELAY MODE This message indicates the air sampler can be programmed to start sampling
after a set period
MULTI MODE This message indicates the air sampler can be programmed to extend the total
sampling time using “sequential interval time” sampling
UTILITY MODE This message indicates the air sampler is programmed to enter the following
Sub-Menu:
SET TIME
To program date and time
SAMPLING SITE
To identify the sampling point
IDENTIFY
To identify the operator’s name
LANGUAGE
To display text in different languages
CLEAR RECORD
To clear the recorded sampling data
DISPLAY RECORD
To show the recorded sampling data
PRINT
To export the recorded sampling data
AUTO SWITCH OFF
To disconnect the automatic switch off when the “Infrared Remote Switch” is
used
MODE
To select aspiration mode
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 9
3.3 PRELIMINARY INSPECTION
The apparatus is subject to specific working tests before shipping and it is carefully packed to avoid possible damage
during transit. However, a visible check should be carried out as soon as possible to determine any transit damage. This
must be reported immediately. The following procedures should be followed to check that the unit is functioning
correctly.
The battery pack of the instrument must be charged for at least 3,5 hours before the test (14 hours if slow battery
charger is used).
ACTION DISPLAY
Switch on the ON/OFF
switch (black button) I N T E R N A T I O N A L
P B I
S. N. X X X X
P R E S S A R R O W S F O R
N E W M E N U S E L E C T .
S . S A S 1 0 0
I D X X X X
S I T E X X X X
S T A R T F O R X X
D / M / Y - H/ M
The motor will run until the figures in brackets reach the same figure as displayed to the right and then it will stop.
During the sampling the two red leds are flashing (see position 16 and 28 in the figures at page 29).
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 10
DUO SAS Super 360
After the ON/OFF button is pressed, an automatic visual presentation appears. In the following display, the entry of
“XX” indicates numerical figures.
ACTION DISPLAY
Switch on the ON/OFF
switch I N T E R N A T I O N A L
P B I
S. N. X X X X
P R E S S A R R O W S F O R
N E W M E N U S E L E C T
D U O S A S 3 6 0
I D X X X X
S I T E X X X X
S E L E C T H E A D
Press ENTER
R I G H T O R L E F T H E A D
R I G H T + L E F T H E A D
The motor will run until the figures in brackets reach the same figure as displayed to the right and then it will stop.
During the sampling the two red LEDs are flashing.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 11
3.4 “55 mm CONTACT PLATES” HOLDER ADJUSMENT
The Contact Plate holders can be adjusted (using a screw driver) in case the available plates slightly different in
diameter from the standard 55 mm Contact Plate (see Figure page 29 and Chapter 4.10 page 23).
4. OPERATIONS
Push the Main switch (black button) once to switch on the unit.
The Main switch (black button) must be pushed twice to switch on the unit if the automatic switch off timer operated
when last used.
Press “CLEAR” at any time to return to the initial “START FOR” configuration.
The air sampler is provided with eight fixed air volumes (“Standard Mode”) stored in the memory and eight
programmable air volumes (“User Mode”) selected by the operator.
The selection of the volume is made by pressing the “UP” or “DOWN” arrows when the program is in the
relevant sub-menu.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 12
SAS SUPER 100, SAS SUPER 180 & SAS ISOLATOR
4.1. TO START WITH THE SAME AIR VOLUME AS THE PREVIOUS SAMPLE
ACTION DISPLAY
Switch on the ON/OFF switch (black
button)- wait 6 seconds S T A R T F O R X X
(Last aspirated volume of air) D / M / Y - H / M
4.1.A - TO START WITH THE SAME AIR VOLUME AS THE PREVIOUS SAMPLE
ACTION DISPLAY
Switch on the ON/OFF switch
S E L E C T H E A D
D / M / Y - H / M
S T A R T F O R X X
D / M / Y - H / M
4.2 TO START WITH ONE OF THE EIGHT MEMORIZED “STANDARD MODE” PROGRAMS
ACTION DISPLAY
Switch on the ON/OFF switch
S T A R T F O R X X
(black button)- wait 6 seconds D / M / Y - H / M
Press “ ” to reach
S T A N D A R D M O D E
D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 13
Press ENTER and then “ ” to select
one of the eight volumes S . P R O G X X X X
(10, 20, 30, 50,100, 200, 500, 1000) D / M / Y - H / M
ACTION DISPLAY
Press ENTER to confirm
S T A R T F O R X X
the chosen figure D / M / Y - H / M
Push “START”
( X X ) X X
D / M / Y - H / M
4.2.A - To start with one of the eight memorized “STANDARD MODE” programs
ACTION DISPLAY
Switch on the ON/OFF switch S E L E C T H E A D
D / M / Y - H / M
” P R E S S S T A R T
D / M / Y - H / M
Push START ( X X ) X X
D / M / Y - H / M
Press CLEAR button to know which head is active (left, right or both) or to change it. The message “SELECT HEAD”
will appear on the visual display. Follow the previous instruction.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 14
4.3 “UTILITY MODE” PROGRAM
In this program the following SUB-MENUS are listed:
Set time; Sampling site; Identify; Language; Clear Record; Display Record; Print; Set Autoswitch.
ACTION DISPLAY
Switch on the Main ON/OFF switch (black
button) and press U T I L I T Y M O D E
“ ” to reach UTILITY MODE. D / M / Y - H / M
ACTION DISPLAY
Press ENTER to select S E T T I M E
the “SET TIME” Sub-Menu D / M / Y - H / M
ACTION DISPLAY
Press ENTER to confirm U T I L I T Y M O D E
D / M / Y - H / M
ACTION DISPLAY
Press ENTER and then “ ” S A M P L I N G S I T E
to select “SAMPLING SITE” Sub-Menu D / M / Y - H / M
Press ENTER S A M P L I N G S I T E
to indicate sampling location X X X X
S A M P L I N G S I T E
X X X X
S A M P L I N G S I T E
X X X X
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 15
4.3.3. “IDENTIFY”
This option is used to identify the operator. This should be changed if different operators use the sampler and especially
if the date is to be printed. The data is recorded in the file “DISPLAY RECORD” (see paragraph 4.3.6).
Select UTILITY MODE Program.
ACTION DISPLAY
Press ENTER and then “ ”
“ I D E N T I F Y “
to select the IDENTIFY Sub-Menu D / M / Y - H / M
“ I D E N T I F Y “
X X X X
“ I D E N T I F Y “
X X X X
4.3.4. “LANGUAGE”
The text of the Menus may be selected choosing from different languages.
Select UTILITY MODE Program.
ACTION DISPLAY
Press ENTER and then “ ”
to select the L A N G U A G E
LANGUAGE Sub-Menu D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 16
4.3.5. “CLEAR RECORD”
This option is used to delete all the data memorized in the “DISPLAY RECORD”. Before starting this procedure,
please be certain that existing data is not required or that it has been downloded.
Select UTILITY MODE Program.
ACTION DISPLAY
Press ENTER and than “ ”
C L E A R R E C O R D
to select the D / M / Y - H / M
CLEAR RECORD Sub-Menu
Press ENTER to delete all the memorised data on the DISPLAY RECORD. After about five seconds the display will
show UTILITY MODE and all memorised data is deleted.
ACTION DISPLAY
Press ENTER and then “ ”
to select the D I S P L A Y R E C O R D
DISPLAY RECORD Sub-Menu D / M / Y - H / M
4.3.7. “PRINT”
The sampler should be connected to the printer by connecting cable (a specific cable and diskette must be ordered: cat.
n. 22536-SAS-PC).
Select UTILITY MODE Program.
Switch the printer on.
ACTION DISPLAY
Press ENTER and then “ ”
P R I N T
to select “PRINT”. Press again ENTER. D / M / Y - H / M
The following data will be printed in chronological order: progressive sample number, day, month, year, hour,
operator’s name, site, litres of aspirated air.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 17
4.3.8. “SET AUTO SWITCH ”
This option is used to disable the automatic switch off option when the Infra Red Remote switch is being used.
ACTION DISPLAY
Press ENTER and then “ ”
to select the “AUTO S E T A U T O S W I T C H
SWITCH OFF” Sub-Menu. D / M / Y - H / M
Press ENTER
A U T O S W I T C H O N
D / M / Y - H / M
Press “ ” to choose
A U T O S W I T C H O F F
D / M / Y - H / M
When the air sampler is switched off at the end of cycle, the Autoswitch off is automatically reset. (For more details see
Chapter 4.9.)
4.3.9. “MODE ”
This option is used to set the aspiration mode of the sampler. The aspiration mode can be chosen between SINGLE
MODE and MULTI MODE. The aspiration mode is recorded in the memory.
Select UTILITY MODE Program.
ACTION DISPLAY
Press ENTER and then “ ”
to select the “AUTO M O D E
SWITCH OFF” Sub-Menu. D / M / Y - H / M
Press ENTER
S I N G L E M O D E
D / M / Y - H / M
Press “ ” to choose
M U L T I M O D E
D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 18
When MULTI MODE is selected the main menù will show alternately the following messages:
S T A R T F O R
D / M / Y - H / M
N C 0 2 X 1 0 0 L .
D / M / Y - H / M
Note: 02 cycle and 100 Liters are just an example, the displayed number are those selected in the MULTI MODE
function.
4.4. To start with one of the eight operator programmable sampling volumes memorised in the “USER
MODE” program
If the operator require to use volumes other than the standards volumes, up to 8 additional volumes, can be program
into the unit.
ACTION DISPLAY
Switch on the ON/OFF switch S T A R T F O R X X
(black button) D / M / Y - H / M
Press “ ” to select U S E R M O D E
the USER MODE D / M / Y - H / M
Press ENTER S T A R T F O R X X X X
to confirm D / M / Y - H / M
ACTION DISPLAY
Switch on the ON/OFF
S T A R T F O R X X
switch (black button) D / M / Y - H / M
Press “ ” to select
P R O G R A M M O D E
PROGRAM MODE D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 19
Press ENTER and then “ ”
S . P R O G X X X X
to select the volume D / M / Y - H / M
of air to be modified
Press ENTER X X X X - 0 0 0 0
D / M / Y - H / M
Press “ ” to select
X X X X - X 0 0 0
the thousands of litres D / M / Y - H / M
Press ENTER. Note the display will still show the old volume before modification.
ACTION DISPLAY
Switch on the ON/OFF
S T A R T F O R X X
switch (black button) D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 20
Press “ ” to select the chosen Delay Time.
The message “DELAY” will flash until the selected time is reached: the display flashes to confirm delayed start has
been selectes.
ACTION DISPLAY
S T A R T F O R X X
* * D E L A Y * *
( X X ) X X
D / M / Y - H / M
This program is very useful for extending the time of sampling with the purpose of obtaining a more representative
environmental sample “in actual operating conditions”. The total air volume to be sampled is aspirated with two or
more sub-volume aspirations (E.g.:1000 litres in ten runs of 100 litres at five minute intervals).
Before entering the MULTI MODE Program you should therefore decide (a) total volume of air to be sampled onto the
Contact Plate; (b) number of runs; (c) interval time. See Application Note N.95 for more details.
ACTION DISPLAY
Switch on the ON/OFF S T A R T F O R X X
switch (black button) D / M / Y - H / M
Press “ ” to select M U L T I M O D E
MULTI MODE D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 21
Press “ ” to select
the first figure S I N G L E R U N V O L .
(thousands of litres) X X X X
ACTION DISPLAY
Press ENTER and “ ” to select
the fourth figure S I N G L E V O L U M E
(units of litres) X X X X
T O T A L L X X X X
Before running the multimode sampling, set the aspirating mode to MULTIMODE (see chapter 4.3.9)
At the end of the first RUN, during the interval, the number of “residual cycles” (runs) that are remaining are shown on
the display:
- X X C Y C L E
* * D E L A Y * *
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 22
4.8. Low Battery Message
When the LOW BATTERY message is flashing, the air sampler must be connected to the battery charger. The residual
charge may be enough to complete the last sample, but this will vary according to the condition of the battery (age, use,
temperature, etc.).
ACTION DISPLAY
Switch on the ON/OFF
! ! L O W B A T T E R Y ! !
switch (black button)
ACTION DISPLAY
Switch on the ON/OFF S T A R T F O R X X
switch (black button) D / M / Y - H / M
Press “ ” to select
U T I L I T Y M O D E
UTILITY MODE D / M / Y - H / M
Press “ENTER”
S E T T I M E
D / M / Y - H / M
Press “ ” to select
S E T A U T O S W I T C H
“SET AUTOSWITCH” D / M / Y - H / M
Sub-menu
Press “ENTER”
A U T O S W I T C H O N
D / M / Y - H / M
Press “ ” to choose
A U T O S W I T C H O F F
“AUTOSWITCH OFF” D / M / Y - H / M
(The instrument will now not switch off after 4/5 minutes and the Infrared Remote Switch will work regularly).
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 23
ACTION DISPLAY
Press “clear” to go S T A R T F O R X X
back to the start D / M / Y - H / M
NOTE.
At the end of this protocol, the automatic switch off option is disabled and the Infrared Remote Switch may be used at
any time.
The automatic switch off option is reactiveted by switching off the air sampler with the Main Switch (black button).
CAUTION!
When the auto switch off option is disabled, remember to switch off the instrument after use to save the battery charge.
The SAS SUPER 100, SAS SUPER 180, DUO SAS 360, SAS ISOLATOR air
samplers are designed for use with standard “Contact Plates”. The plate holders are
adjustable to allow different brands of Contact Plate to be fitted.
Adjustable plate holders in the sampler head allow the operator to use contact
plates from many sources without concern for slight differences in plate size.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 24
4.12. “Contact Plate” handling
(a) Disinfect the cover of the air sampler at the beginning of each sampling program treating the inside and the outside
part with a disinfecting alcohol wipe or swab.
(b) The cover with its protective plastic lid can be autoclaved at 121°C for 15 minutes. If this procedure is to be
repeated regularly, a s/s head is recommended. It is therefore useful to have available several heads ready to use.
Alternatively, the unit can be sanitised using a disinfecting aerosol at about 30 cm from the head when the fan is
running. Thirty seconds are sufficient to disinfect the air path.
(c) Remove the cover of the air sampler by unscrewing it, holding the edge of the cover. Avoid touching the inside and
outside of the drilled area.
(d) Insert a closed filled “Contact Plate” into the retaining slots and then remove its lid. Avoid contamination from
droplets and aerosol infection.
(e) Replace the sampling head and start the air aspiration cycle. At the end of the cycle, unscrew the sampling head,
replace the “Contact Plate” lid and remove the “Contact Plate”.
(f) Be sure each “Contact Plate” is identified with the appropriate sample data.
(g) Incubate the “Contact Plate” for the specified time at the appropriate temperature.
(h) At the end of incubation count the number of visible organisms (C.F.U.) and relate this number to the volume of
air sampled.
Note.
The air sampler is a scientific instrument and its surface should be disinfected using an aerosol and not a liquid!
The unit should be protected with a sterile bag and then transferred into the Clean Area.
It is important to wear sterile gloves when handling the “Contact Plates” and the head of the air sampler to prevent
bacteriological contamination.
Use double packed, gamma irradiated “Contact Plates” to avoid possible clean environment contamination.
For more details see Application Notes N.15, 31, 55, 63, 68, 83, 95, 99.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 25
4.14. “Contact Plates” preparation
It is important that the volume of media dispensed into a “Contact Plate” is constant for the type of plate used. An
excess volume can create a convex surface that is too high and which may touch the inside of the sampler head. An
insufficient volume will create a concave surface, unsuitable for representative colony growth using SAS System.
The volume of agar in a “55 mm Contact Plate” should be 16-18 ml and in a ”84 mm Maxi-Contact Plate” 24-26 ml.
For more details see Application Note N.78.
AREA Position SAS s/n Single Air Total Air Sub Sample Sampling Start delay
Volume Volume number interval
A A1 1.210 - 1.000 - - 5
A2 1.211 200 1.000 5 30 -
A3 1.213 250 1.000 4 30 -
B B1 1.214 250 1.000 4 30 -
B2 1.215 200 1.000 5 30 -
C C1 1.216 - 1.000 - - 20
(a) Air sampler SAS SUPER 100 Serial Number 1210 was positioned in AREA A on site A1 and programmed for a
total air volume of 1000 litres in one run, starting after 5 minutes from the switching on of the instrument.
(b) Air sampler SAS SUPER 100 Serial Number 1211 was positioned in AREA A on site A2 and programmed for a
total air volume of 1000 litres in five times (200 litres during each run) with interval times of 30 minutes.
(c) Air sampler SAS SUPER 100 Serial Number 1213 was positioned in AREA A on site A3 and programmed for a
total air volume of 1000 litres in four times (250 litres during each run) with interval times of 30 minutes.
(d) Air sampler SAS SUPER 100 Serial Number 1214 was positioned in AREA B on site B1 and programmed for a
total air volume of 1000 litres in four times (250 litres during each run) with interval times of 30 minutes.
(e) Air sampler SAS SUPER 100 Serial Number 1215 was positioned in AREA B on site B2 and programmed for a
total air volume of 1000 litres in five times (200 litres during each run) with interval times of 30 minutes.
(f) Air sampler SAS SUPER 100 Serial Number 1216 was positioned in AREA C on site C1 and programmed for a
total air volume of 1000 litres in one run, starting after 20 minutes from the switching on of the instrument.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 26
5. FINAL OPERATIONS
Switch off the ON/OFF Main switch (black button).
The unit will switch off automatically after 4/5 minutes, if the “switchg off option” has not been disabled for Infrared
Remote Control use.
6. CALCULATION OF RESULTS
Colonies MPN Colonies MPN Colonies MPN Colonies MPN Colonies MPN Colonies MPN
r Pr r Pr r Pr r Pr r Pr r Pr
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 27
CORRECTION TABLE TO ADJUST COLONY COUNTS FROM A 487-HOLE IMPACTOR USING 84 mm
MAXI-CONTACT PLATES
Colonies MPN Colonies MPN Colonies MPN Colonies MPN Colonies MPN Colonies MPN
r Pr r Pr r Pr r Pr r Pr r Pr
Pr x 1000
X =----------------------
V
Where:
V = Volume of sampled air = 200 litres of air
r = Colony Forming Units counted on “ 55 mm Contact Plates” = 67
Pr = Probable count obtained by positive hole correction = 80
x = Colony Forming Units per 1000 litres (= 1 cubic metre) of air
80 x 1000
x = ------------------------- = 400 CFU per 1000 litres of air (1000 litres=1m3)
200
To express the final result in CFU/ft3 multiply the CFU/m3 value by 0,02832
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 28
SECTION THREE
7. SPECIFICATIONS
Principle:
Air-borne micro-organisms are collected on microbiological agar by impaction produced by aspiration.
Size: 105x110x290 mm
Weight: 1750 g
Construction:
Aspirating Head: aluminium or stainless steel
Turbine: antistatic plastic resin
Housing: polyurethane resin
Sampling Efficiency:
The effective sampling efficiency of the SAS SUPER 100, in a controlled environment, with aerosol of known particle
size is 100% over 4 microns in size.
Considering the normal range of environmental micro-organisms is between 4 and 20 microns, the SAS SUPER 100 is
suitable for all normal air monitoring testing ([Link] – Journal of Hospital Infection, 1985, 6, 102-107). This
condition is obtained using the standard 219 holes aspirationg head.
If, for special applications or research, it is necessary to collect micro-organisms to a size of 1 micron, the 401 holes
special aspirating head should be used.
This head has smaller holes to increase the velocity of particles and achieve a 100% capture efficiency on particles
down to 1 micron in size.
Acoustic signal:
An acoustic signal alerts the operator when air sampling is completed.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 29
SAS Super 100 / SAS Super 180 / SAS Isolator Contact Plate
Chamber
SAS Super 100 / SAS Super 180 / SAS Isolator Front Control Panel
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 30
7.1 Messages shown on display
START FOR XX The instrument is ready to collect the same volume of air as the last sample
STANDARD MODE 8 Standard volumes option
USER MODE 8 User volumes option
PROGRAM MODE Program to modify user program
DELAY MODE Program to delay sampling start:
1, 2, 3, 5, 10, 20 minutes
MULTI MODE Program for sequential interval sampling: interval time, numbers of runs, single run
volume, total volume of aspirated air,
** DELAY ** The sampler is in “stand-by”, waiting to start
UTILITY MODE Program to set time, sampling site, operator’s identification, language, clear record,
display record, print, auto switching off
**LOW BATTERY** The battery of the instrument must be recharged
When the message LOW BATTERY is flashing, the air sampler must be recharged.
1. Switch off the unit.
2. Connect the smaller plug of the battery charger to the battery socket on the SAS SUPER 100 or SAS SUPER 180.
3. Connect the battery charger to the main supply (220-240 or 110 Volts): verify that the LEDS on the charger are lit
correctly (see label on charger).
4. The air sampler is recharged after 14 hours using the normal battery charger “Revit“ (Cat.n.18201 or 19248) or
after 3.5 hours using the fast battery charger “Swift” (Cat.n.17275 or 19143).
5. Disconnect the battery charger from the mains supply.
6. At the end of operation, disconnect the battery charger plug from the socket of the air sampler.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 31
NOTES
(a) The correct metal hydrate battery charger must be used to charge the SAS SUPER 100 or SAS SUPER 180!
(b) If the air sampler has not been used for several weeks, check the battery status!
(c) The efficiency of the battery is reduced when operating at temperatures below 0°C.
(d) This battery pack, using the SAS SUPER 100 sampler, should provide approximately 5-7 hours of sampling time.
(50% less in SAS SUPER 180).
Before using for the first time, or after a storage time of one month or more, charge battery for 14 hours. If you do
not reach the rated battery capacity during initial use, repeat the 14 hours charge process.
Do not charge the battery for more than 24 hours.
Battery performance will degrade at extreme temperatures like +60° or – 20°C.
The Metal Hydrate battery has a self-discharge rate and, without use, will lose about 2% of its charge per day.
To prevent injury or burns, do not allow metal objects to contact or short circuit the battery terminals.
The Metal Hydrate battery may explode if disposed in fire.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 32
SECTION FOUR
8. MAINTENANCE
8.1 Cleaning
The SAS SUPER 100, SAS SUPER 180, DUO SAS 360, SAS Isolator do not require special care and maintenance
procedures. Clean the outside surface with a normal mild NON CORROSIVE detergent.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 33
8.5 Battery replacement
1. Take out the two caps (Position 1) from the lower part of the control panel.
2. Unscrew the three fixing screws (Position 2) that fix the control block.
3. Slide the control block (Position 5) from the instrument body (Position 6).
Accessories
Cat.n. 15931 55 mm aluminium certified drilled head
Cat.n. 15503 55 mm stainless steel certified drilled head
Cat.n. 19123 84 mm aluminium certified drilled head – complete with adaptor for
Maxi Contact Plate
Cat.n.18148 84 mm stainless steel certified drilled head – complete with adapter for Maxi Contact Plate
Cat.n.19117 90 mm aluminium standard Petri dish head + adaptor
Cat.n.19119 90 mm stainless steel standard Petri dish head + adaptor
Cat.n.19115 Atrium chamber for Clean Room application
Cat.n. 6064 Autoclavable plastic cover for sampling head
Cat.n.19239 Compressed air head
Cat.n. 5214 Table tripod
Cat.n. 5215 Floor tripod
Cat.n.18201 Normal battery charger (220 Volt)
Cat.n.19248 Normal battery charger (110 Volt)
Cat.n.86603 Fast battery charger (220 Volt)
Cat.n.86604 Fast battery charger (110 Volt)
Cat.n.20153 Aluminium carrying case
Cat.n.18143 Soft carrying case
Cat.n.18950 Infrared remote control
Cat.n.18200 “IN OUT MH” Battery pack
Cat. n.20915 “SAS Printer” Printer to be connected to SAS Super 100
Cat.n.19836 “Paperol” paper for SAS printer
Cat.n.19834 “ARM” System to fix SAS Super 100 to wall
Cat.n.20093 Installation Qualification manual
Cat.n.20094 Operator Qualification manual
Cat.n. 22536 SAS Software
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 34
Control and Validation Systems
Cat.n.86666 Pyramid
Consumables
“AGAR CONTACT” READY TO USE MEDIA FILLED STERILE CONTACT PLATES
SECTION FIVE
9. BRIEF INSTRUCTIONS FOR SAS SUPER 100, SAS SUPER 180, SAS Isolator
ACTION DISPLAY
Switch on the ON/OFF switch (black
button) S T A R T F O R X X
(Last aspirated volume of air) D / M / Y - H / M
Push “START” ( X X ) X X
(after the visual display presentation) D / M / Y - H / M
ACTION DISPLAY
Switch on the ON/OFF switch S T A R T F O R X X
(black button) D / M / Y - H / M
Press “ ” to select S T A N D A R D M O D E
D / M / Y - H / M
Push “START” ( X X ) X X
D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 35
TO START WITH ONE OF THE EIGHT FREE SAMPLING VOLUMES PRE-MEMORISED
IN THE “USER MODE” PROGRAM
ACTION DISPLAY
Switch on the ON/OFF switch
S T A R T F O R X X
(black button) D / M / Y - H / M
Press “ ”
U S E R M O D E
to select the USER MODE D / M / Y - H / M
Press “ENTER”
S . P R O G X X X
to select the program D / M / Y - H / M
Press ENTER
S T A R T F O R X X X X
to confirm D / M / Y - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 36
9.1 BRIEF INSTRUCTIONS FOR DUO SAS SUPER 360
To start with the same air volume as the previous sample
P R E S S S T A R T
D D / M M / Y Y - H H / M M
P R E S S S T A R T
D / M / Y - H / M
Push START
( X X ) X X
D / M / Y - H / M
Press CLEAR button to know which head is active (left, right or both) or to change it. The message “SELECT HEAD”
will appear on the visual display.
Follow the previous instruction.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 37
SAS SUPER 100 KURZANLEITUNG
Einschalten am schwarzen
Ein-/Ausschalter Starten mit xx
(letztes Ansaugvolumen) T/M/J – Std / Min
Einschalten am schwarzen
Ein-/Ausschalter Starten mit xx
(letztes Ansaugvolumen) T/M/J – Std / Min
Einschalten am schwarzen
Ein-/Ausschalter Starten für xx
(letztes Ansaugvolumen) T/M/J – Std / Min
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 38
INSTRUCCIONES RESUMIDAS
ACCIÓN DISPLAY
Conectar el interruptor ON/OFF
(Botón negro) S T A R T F O R X X
(Último volumen de aire aspirado) D / M / A H / M
Pulsar “START”
( X X ) X X
(Después de la presentación en el display) D / M / A H / M
ACCIÓN DISPLAY
Conectar el interruptor ON/OFF
S T A R T F O R X X
(Botón negro) D / M / A - H / M
Pulsar “ “
S T A N D A R D M O D E
Para seleccionar D / M / A - H / M
STANDARD MODE
Pulsar “START”
( X X ) X X
D / M / A - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 39
FUNCIONAMIENTO CON UNO DE LOS OCHO VOLÚMENES DE MUESTREO PREFIJADOS
LIBREMENTE EN EL PROGRAMA “USER MODE”
ACCIÓN DISPLAY
Conectar el interruptor ON/OFF
S T A R T F O R X X
(Botón negro) D / M / A - H / M
Pulsar “ “
U S E R M O D E
Para seleccionar D / M / A - H / M
USER MODE
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 40
INSTRUCTIONS RESUMEES
ACTION ECRAN
Allumer l’interrupteur ON/OFF (bouton C O M M E N C E R P A R X X
noir)
(dernier volume d’air aspiré) J / M / A - H / M
ACTION ECRAN
Allumer l’interrupteur ON/OFF C O M M E N C E R P A R X X
(bouton noir) J / M / A - H / M
M O D E S T A N D A R D
Presser « » pour sélectionner le mode
J / M / A - H / M
d’utilisation
( X X ) X X
Appuyer sur la touche «START»
J / M / A - H / M
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 41
POUR PRELEVER 1 DES 8 VOLUMES AU CHOIX DE L’UTILISATEUR DANS LE «MODE UTILISATEUR»
(LES VOLUMES DOIVENT ETRE PRE-ENREGISTRES DANS LE «MODE UTILISATEUR»
ACTION ECRAN
Allumer l’interrupteur ON/OFF C O M M E N C E R P A R X X
(bouton noir) J / M / A - H / M
C O M M E N C E R P A R X X X X
Presser la touche «ENTER» pour J / M / A - H / M
confirmer
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 42
SECTION SIX
10. AIR SAMPLER IDENTIFICATION SHEET ACCORDING TO ISO EN 17025
According to the Document ISO EN17025, to apply the current Good Laboratory Practice, it is necessary to identify
each instrument with a specific sheet. An example is here reported.
Form n. XXXX
Date XXXX
Company / Institute XXXX
Laboratory XXXX
Name of Instrument Microbiological Air Sampler
Model SAS SUPER 100
Serial Number XXXX
Name of Producer International pbi
Name of Distributor XXXX
Condition at the moment of arrival (X) new; (x) used; (x) reconditioned
Date of Arrival / /
Date of initial use / /
Usual collocation Room XXXX
Servicing details See servicing POS
Instrument to be calibrated (X) Yes; (X) No
Periodic Calibration (X) monthly; (X) quaterly; (X) Yearly
Instrument to be validated (X) Yes; (X) No
Periodic Validation (X) half year; (X) yearly
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 43
SECTION SEVEN
11. SAS SUPER ISOLATOR SCHEMATIC
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 44
To the attention of: Technical department International pbi S.p.A.
Via Novara, 89 - 20153 - Milano - Tel + 39 02/48.779.1-Fax +39 02/400.900.10
AUTHORISATION RETURN NUMBER TO BE ASKED TO THE EXPORT DPT
Company:
Address:
City: Zip Code State
Phone Fax Email
Name to contact:
Lab/Division:
return request of technical service
Equipment:
Model Serial Number:
for calibration repair other (specify)
NOTE OF MALFUNCTIONING
ELECTRICAL PART
ELECTRONICAL PART
MECHANICAL PART
HYDRAULICS PART
NOTES:
Mod. PQ09.9
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 45
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 46
WARRANTY
WARRANTY CONDITIONS
International pbi warrants this product to be free of defects in materials and functionality
The repairs will be effected by International pbi or by authorized dealers as follows:
ATTENTION
TO OUR NEW CUSTOMER
As a user of our equipment you are covered for a 12 months guarantee for all spare parts as a result of mechanical
failure of the machine during normal use. In order to provide you with first class service, it is essential for us to know
all your references and which equipment you have. We kindly ask you therefore to fill in the attached guarantee and
return the card to us by first class mail.
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 47
DECLARATION OF CONFORMITY
HEREBY DECLARES
under its sole responsibility that the following product
Il Presidente
Roberto Ligugnana
Milano 2006
International pbi [Link] « Sas Super 100/180, Duo Sas 360, Sas Isolator » September 2006 Rev. 5 48