JUCLEIC ACIDS TO MEC! \ DNA molecule in which both strands have radioactive thymidine is allowed to duplicate in an environment containing non-radioactive thymidine, What will be the exact number of DNA molecules that contain some radioactive 1¢ after 3 duplication ? thymid (1) One (3) Four 2) Two (4) Eight Enzyme, that is not participates in the process of DNA-replication in prokayotes :~ (1) DN, (2) DNA topoisomerase ligase (3) Reverse transcriptase (4) DNA polymerase Which of the following is not a characteristi feature of genetic material ? (a) It should be chemically and structurally less stable (b) It should be able to produce its replica (c) It should be more reactive (d) It does not follow Mendelian inheritance (c) It provides scope for slow changes ie. mutation (1) only a,b, ¢ and d (2) b, de Bede (4) acd Viruses having RNA genome evolve faster, because :~ (1) RNA is comparatively unstable, so mutate at a faster rate (2) RNA is stable, and mutate at a slow rate (3) RNA is chemically less reactive (4) RNA is double stranded DNA is better genetic material than RNA, because:- (1) DNA is chemically less reactive and structurally more stable as compared to RNA (2) Presence of thymine at the place of uracil provides additional stability to DNA (3) DNA being stable, so mutate at a slow rate (4) All the above 6. 10. it, HANISM OF DNA Consider the following four statements 4 and D and select the right option for tw ae statement := Statements :~ (A) RNA was first genetic material (B) S-methyl uracil is found in RNA, (C) DNA polymerase-Il doesnot require RX, primer for the synthesis of DNA stany (D) RNA primer is formed durin; of DNA replication ‘The correct statements are :- (1) (A) and (B) (2) (B) and (C) (3) (A) and (D) (4) (A) and (C) If Excoli containing N'°-N' DNA was allowed 10 grow for 80 minutes in medium containing N' then, what would be the percentage of lighy density and hybrid density DNA molecule 2 (1) 87.5% light density ; 12.5% hybrid density (2) 75% light density ; 25% hybrid density (3) 25% light density ; 75% hybrid density (4) 12.5% light density ; 87.5% hybrid density Which is not found in DNA ? (1) Deoxy ribose sugar 8 the Process (2) S-methyl uracil (3) Demethylated thymine (4) H,PO, Viruses having RNA genome evolve faster, because :- (1) RNA is unstable and mutate at a faster rate (2) RNA is more stable (3) RNA is first genetic material (4) RNA is the main geneti A segment of DNA molecule contains 20,000 base pairs, What is the number of phosphate molecule in this DNA molecule ? (1) 20,000 (2) 40,000 (3) 10,000 (4) 5,000 In a segment of DNA molecule there occurs 200 molecules of adenine nitrogen bases and 200 molecules of cytosine nitrogen bases. What will be the total number of purine nitrogen bases in this segment ? (1) 800 (3) 100 ¢ material (2) 400 (4) 200111 Histone Histone ‘octamer e octamer is made uy on ote POLS types of ()) gqone protein is Hi ypical nucleosome contains Ay : OONA Helix jstones are OTEANISEd 19 form, : (Ont molecules called ag 200 bp of Unit of as histone octamer ¢ negatively charged DNA i, om ind the positively charged histon, —_ a structure called nucleo, © “rapped © Octamer ome, statements are correct 9 (2) Two (4) Four g) Three t ch one is tTUe among the follow ee Pe ican nA A ing for 3 ¢ » Nee . \=, 3) iy * ( eo Guar In a phosphodiester bond formation t Renate group is bonded with r 5-OH of one sugar and 2'-OH of next (ys sugar / : 2) J-OH of one sugar and 5'-OH of next 3 sugar : 3) 3'-OH of one sugar and 2'-OH of next 33 sugar 4) 9-OH of one sugar and 1'-OH of next sugar Which of the following enzyme inhibit transformation of R-strain bacteria to $-strain:— (1) DNAs (2) RNase (3) DNase (4) Both (1) and (3) 16, kazaki fraget, ; i oh “Apable of (ranslation rein PNA which Undergo ) Seeme oe f chain of Ucleotides formed (4) s, Pen oh a Of chain of Ucleotides formed . hee ae of DN, SAN IS knoy, hen et eter nthe then the Sequence in other * Predicted, this ig because of :- hature 18, nucleic acid ‘at it Contained the N, bases ion A = 20%. T = 15%, 30%, This data indicates s that — id in he sample is single (3) Nucleic acid in the s; Stranded RNA. (4) Nucleic acid in the sa Stranded RNA. Which one is Incorrect statemer material ? ample is double imple is single 19, nt about genetic (1 It should be able to express itself inthe form of "Mendelian characters" (2) DNA chemically tess. reactive structurally more stable than RNA (3) RNA can directly code forthe synthesis of Proteins (4) Only DNA is able to mutate 20. IF E coli with heavy DNA is allowed to grow for 80 minutes in “NH,CI medium then what would be the proportion of hybrid and light density DNA molecules respectively :- (7st 2)1:7 (3)2:0 0:2 andThe following features occur during pretein synthesis: (i) Movement of m-RNA from the nucleus into the cytoplasm, where it binds to the ribosomes (ii) Formation of amino acyl -RNA complex (iii) Transcription of specific segments of DNA to M-RNA molecules in the nucleus (iv) Binding of N-terminal amino methionine t-RNA to the 'P* site of the ribosome, The CRNA anticodon "pairs with AUG on m-RNA" (v) Formation of peptide bond between first and second amino acids at the ‘A’ site (vi) Release of the completed polypeptide chain from ribosomes (vii) Migration of dipeptide to form a tripeptide and then a polypeptide The correct order of events is (1) Gili) = Gi) = G) = Liv) = (Y) = (vi) = (vii) (2) G) - Ci) = Gili) = Civ) - (v) = (i) - Wii) (3) (iii) ~ (i) ~ Gi) = ivy - (w) ~ Wi) - Wid) (4) (iiiy = (i) — Gai) - Civ) - (v) = (vii) - (Vi), TMV infects a variety of plants, including Zinnia and tobacco. When TMV-RNA is used for infection, the amino acid sequence of the coat protein of the progeny is the same whether the host is Zinnia or tobacco. This proves that te (2) universal (3) unidirectional (4) non-overlapping Transfer RNA = (1) forms hydrogen bonds between its codon and the anticodon of an m-RNA in the ‘A’ site of a ribosome (2) binds specific amino acid by the help of aminoacyl tRNA synthetase (3) uses GTP as the energy source to bind its amino acid (4) is translated from m-RNA Several proteins may be produced at the same time from a single m-RNA by = (1) the action of several ribosomes in a string called a polyribosome (2) several RNA polymerase molecules working sequentially 10. (3) single peptides that associate Fibosomes with rough E.R, (4) the involvement of multiple spliceosome, complex Which of the following is true for RN, processing ? (1) Exons are excised before the m-RNA processing ) Assemblies of protein and saRNPs, calleg spliceosomes, may catalyze splicing (3) ‘The RNA transcript that leaves the nucleus may be much longer than the original transcript (4) Large quantities of rRNA are assembled into ribosomes. All of the following would be found in a prokaryotic cell except :- (1) m-RNA’ (2) rRNA (3) simultaneous transcription and translation (4) sn-RNA A functional unit of gene which speciefic the synthesis of one polypeptide is known as : (1) recon (2) clone (3) codon (4) cistron A DNA strand is directly involved in the synthesis of all the following except (1) CRNA molecule (2) m-RNA molecule (3) another DNA strand (4) protein synthesis Because most of the amino acids are represented by more than one codons, the genetic code is said to be (1) deaminated (2) comma less (3) degenerate Which of the following RNA has structural and catalytic role in translation ? (1) m-RNA (2) URNA (3) FRNA (4) g-RNA (4) overlapping13. Ribosome Match the column-I with column-it and select the correct answer :- Column-1 Replication Transenption Translation Column-I1 RNA > DNA a (u) | m-RNA~protein DNA ~ mRNA PORN ONAN (iii) (iv) Reverse transcription (9) | FRNA ¢ protein (I) A-i, B~ii, C iii, D iv, Bey (2) A- ii, B- i, C - ili, D - iv, Eey (3) A - iii, B - iv, C -ii, D - i, Ev (4) A - iv, B - ili, C - ii, D - i, E-v The same amino acid sequence ofa polypeptide chain can be represented by more than one type of nucleotide sequence in mRNA. It explains which property of genetic code? (1) Universal nature (2) Degeneracy (3) Un-ambiguous (4) Comma-less or continuous The process of translation requires the transfer of genetic information from :- (1) A polymer of amino acids to a polymer of carbohydrates. (2) A polymer of deoxy nucleotides to @ polymer of nucleotides. (3) A polymer of nucleotides to a polymer of amino acids. (4) A polymer of amino acids to proteins. Which of the following region of transcription nit is located towards 3' end of the coding strand and it usually defines the end of the process of transcription ? (1) Promoter (2) Structural genes (3) Terminator (4) Coding strand 17. 3! P= Promoter 5! 1 = Terminator The m-RNA produced by transcribtion of above given DNA is :- (1) S' AUGCU 3° (2) 5' AGCAU 3° (3) 3, UCGUA 5' (4) 5’ UACGA 3' Which statement is true for transcription? (1) Both strands of DNA are copied during transcription (2) The total DNA of an organism gels transcribes (3) Only a segment of DNA and only 0 the strands is copied into RNA (4) RNA dependent DNA polymerase enzyme ne of catalyses process of transcription Which of the following codon has dual 9 functions ? (1) UUU (2) UGA (3) AUG (4) UGG Which of the following RNA act as enzyme for the formation of peptide bond in bacteria :- (1) 285 rRNA (2) 23s rRNA (3) 16s RNA (4) 188 FRNA Which of the following enzyme is helpful in polymerising RNA with defined sequences in a template independent manner? (1) Severo ochoa enzyne (2) Polynucleotide phosphorylase (3) DNA dependent RNA polymerase (4) Both (1) and (2)e following statement ! n? estructural genes, Which of th with respect to Lac operon (1) Single promoter for thre y, lac a RNA formation duction of Lac operon lac z. lac y (2) Polycistronic ™ (3) Glucose results in 1M n shows negative control | DNA, replacement of In structure of normal nis base by another nitrogenous lacement of adenine n the above (4) Repressor protei one nitrogeno pase is called as ‘A’ and re by guanine is called as 'B'. I statement A & B here refers t0 = B = Transition (As Substitution (2) A > Point mutation B > Transversion @)A > Frame shift mutation B > Transversion (4) Both (2) and (3) In the octaploid wheat, the haploid(n) and basic numbers(X) of chromosomes, are‘ (yn =21X=7 (2)n=7, @Q) n= 28, X=7 @)n=7 A classical example of point mutation is a change of single base pair in the gene for beta globin chain that results in the change of amino acid residue glutamate to valine. It result into a diseased condition called as = (2) Phenylketonuria (4) Thalassemia X=21 X= 28 (1) Sickle cell anemia (3) Haemophila Select the incorrect statement for RFLP :- (A) It is the basis of DNA fingerprinting (2) Ibis due to variable length of restricted fragment (3) Ibis due to variable number of minisatellite. (4) It is same for all human beings. Any Allelic sequence variation has traditionally been described as DNA polymorphism if more than one allele at a locus show : - (J) Frequency greater than 0.01 (2) Frequency less than 0.01 (3) Frequency greater than 1.0 (4) Frequency less than 0.01% me 10. Find an incorrect statement with respec , results of HGP :- 0 dd) Dystrophin protein gene, is the largest ene of human genome. (2) Chromosome Y has minimum 231 gene, (3) SNPs are identified at about 2.4 millon locations (4) The functions are unknown for over 599, of discovered genes. If there are four different types of nitrogeneous, 5 (A. T. Gand C) then how many differen, base: types of transitions and transversions are possible? (I) Transition = 8, Transversion = 4 (2) Transition = 4, Transversion = 4 (3) Transition = 8, Transversion = 8 (4) Transition = 4, Transversion = 8 Choose the incorrect match w.r.t. HGP : (1) Expressed Sequence Tags - Identifying all the genes that are expressed as RNA (2) Sequence Annotation - Sequencing both coding and non-coding sequences then ening different regions with functions (3) YAC and BAC - Cloning vectors (4) Human chromosome number 22 - The last chromosome to be sequenced Which one is correct for variable number tandem repeat (VNTR) : (1) Is a location in a genome where nucleotide sequence is organised as tandem repeat (2) The number of repeat varies from chromosome to chromosome in an individual and also between individuals (3) Its size varies from 0.1 to 20 kbp (4) All are correct