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Reparacion Microscopio 2

This document serves as an introduction to the Nikon Labophot microscope, covering its components, operation, Koehler illumination, oil immersion techniques, and preventive maintenance. It includes detailed instructions for using the microscope, troubleshooting common issues, and maintaining its parts. The program is designed for beginners and emphasizes hands-on practice alongside the instructional videotape.

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0% found this document useful (0 votes)

94 views101 pages

Reparacion Microscopio 2

Uploaded by

Victor gonzalez

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

Introduction to the Microscope

Operation and Preventive Maintenance

using the Nikon Labophot
All rights reserved. No part of this program may be reproduced
in any form without written permission from Nikon Inc. and
The University of Texas Health Science Center at Houston

RUNNING TIME: 26:00 minutes

Table of Contents Videotape Counter

Reading

|. Components 29
Il. Operation 195
Ill. Koehler Illumination 275
IV. Oil Immersion 343
V. Preventive Maintenance 400
VI. Trouble Shooting (This section is not included
in the videotape)

This program is intended as an introduction to the compound binocular microscope. No prior microscopic
experience is required. It is recommended that this videotape be first viewed in it’s entirety; then each
section can be repeated for practice and review. Videotape counter readings are provided for this purpose.
Counter readings are approximate and may vary according to the equipment being used. To begin, zero the
meter upon appearance of the Nikon logo. Do not reset counter between sections. Also, it is beneficial for
the viewer to have access to a microscope while viewing the videotape.
|. Components
Diopter Rings
Rubber Eyeguard ‘Adjust for focusing difference
Adjust for comfort = between eyes

Eyepiece Tube Clamp Screw

Loosen to rotate head

Reverse Facing Nosepiece

For ease in specimen manipulation
Revolving Nosepiece
Use to rotate objectives Coarse Focus Knob
Brings slide into view
Objectives
Lenses which form primary image of
specimen Fine Focus Knob

Field Diaphragm Sharpens Image

Aperture diaphragm which restricts
area of illumination
= Lamp Socket
Field Diaphragm Control Ring Holds light source
Adjusts size opening of field
diaphragm

interpupillary Distance Scale Eyepieces!

Indicates distance
A 10x magnification
between eyes
Rotate to adjust for interpupillary
E distance;
È w Magnify image (x 10) formed by
objective lens

Stage
Holds Specimen

Slide Holder
Holds slide in place Stage Clamp Screw
Loosen to remove stage

Condenser Control Ring

‘Adjusts size opening of condenser
X/Y Travel Knobs
Condenser
Moves slide on stage Aperture diaphragm that controls
light

Condenser Centering Screws

Centers the field of view

Brightness Control Dial

Condenser Focus Knob Turns microscope on/off;
Focuses light onto slide Adjusts light intensity
II. Operation
1. With the stage toward you, rotate head into viewing position.

2. Plug power cord into electrical socket.

3. Turn microscope on with brightness control dial.

4. Place slide on stage (cover slip facing up) and secure it with slide holder.

5. Move specimen into light path with X/Y travel knobs.

6. Rotate to 10x objective

7. Adjust light intensity until it is at a comfortable setting for your eyes

8. Rotate eyepieces to set interpupillary distance so that the right and left images merge
into one.

9. Focus on image with coarse and then fine focus knobs.

10. Select a good viewing area using X/Y travel knobs.

11. Holdmilled ring on eyepiece stationary.

12. Rotate eyepieces until end surfaces are on white line or “0”.

13. Refocus with fine adjustment knob.

14. Check focus on next highest magnification (40x).

15. Return to lowest magnification (4x).

16. Hold milled ring on eyepiece stationary.

17. Rotate eyepieces to adjust for diopter difference (focusing difference between eyes),

Information on Objectives To Calculate the Final Magnification

Magnification of Magnification of Final

the Eyepiece x the Objective you = — Magnification
Color coded ring are using
Type of objective Primary magnification
Immersion liquid 10 É 40 = 400
Numerical aperture
Mechanical tube length Cover glass thickness y
Ill. Koehler illumination
Koehler illumination is a process which provides optimum contrast and resolution by focusing and centering
the light path and spreading the light evenly over the field of view.

Rotate to 10x objective.

Close condenser aperture diaphragm by turning the condenser control ring to the right.

Raise condenser with condenser focus knob.

Close the field diaphragm to its smallest opening with the field diaphragm control ring.

Focus the field diaphragm image with the condenser focus knob until the edges of the
octagonal image are sharp and have a reddish pink tinge

Center the field diaphragm image with the condenser centering screws. This centers
the light path onto the specimen plane.

Switch to desired objective.

Enlarge field diaphragm image toward edge of the field of view with the field diaphragm
control ring.

Recenter the field diaphragm image.

10. Further enlarge the field diaphragm image until it is just outside the field of view. This
provides enough light to fully and evenly cover the observation area.

11. Remove the right eyepiece and look down the eyepiece tube. The circular beam of
light is the image of the condenser.

12. Adjust the condenser diaphragm with the condenser diaphragm control ring until the
beam fills 3/4 of the tube (1/4 of the area of the tube will be dark and 3/4 will be light).
This provides optimum contrast and resolution.

1/4 dark
13. Replace eyepiece.
3/4 light
14. Adjust light intensity with the brightness control dial.
IV. Oil immersion
Oil should never come in contact with the dry lenses such as the 4x, 10x or 40x objectives or be placed on
the slide until the viewing area has been chosen.

Rotate to 10x objective.

Select viewing area.

Rotate 10x out of light path.

Place drop of immersion oil above beam of light.

Watching from the side of microscope, rotate to the oil immersion objective. Do not
allow dry objectives to pass through the oil.

Adjust the fine focus.

If necessary, center and focus the field diaphragm image.

Enlarge field diaphragm image.

Remove eyepiece.

10. Adjust condenser diaphragm to fill 3/4 the tube.

11. Replace eyepiece.

12. Adjust light intensity. More light is required at higher magnifications.

13. Observe specimen.

14. After you are finished, clean lens and slide. Remove excess oil by blotting with clean
lens paper. Continue cleaning with 1-3 drops methanol. Start from center of lens and
move in a circular motion towards outside of the lens. Gently blot the lens dry. Never
rub the lens because of foreign particles possibly being present.
V. Preventive maintenance

To replace bulb:
1. Turn off and unplug microscope prior to replacing bulb. Pull lamp socket out of the
microscope base and remove the old bulb from its socket.

2. Do not handle new bulb with fingers. Use the protective packet and place the bulb
pins directly into the socket. Insert socket into the base.

To clean optics:
3. Dust eyepieces, condenser, field lens and filters often with a camel's hair brush or
bursts of air. Clean the eyepieces and objectives with lens paper only when
finger prints or smudges appear or when dusting does not seem to correct a problem.

4. Clean the oil immersion lens at the end of each day's use with lens paper and
periodically with methanol or lens cleaning solution.

5. If objectives get immersion oil or other fluids on them clean immediately. First slot
the lens with clean lens paper to remove fluid. Dampen lens paper with 1-3 drops of
methanol and use the procedure described in the oil immersion section. Finally blot
the lens dry.

To inspect objectives:
y 6. Carefully unscrew the objective from its socket, keeping one hand underneath the
objective to prevent it from falling. Remove one eyepiece and invert it; it then becomes
sk a high powered magnifying glass. Aim it toward the lens at a 45 degree angle until the
Z ς image of the lens is in focus. A clean lens should sparkle.

7. Use xylene to remove stubborn smudges. Moisten a cotton tipped swab in xylene and
apply sparingly to the optic in a circular motion. Use extreme caution with xylene
because it can soften the lens cement and cause severe damage.

To store microscope:
8. Keep microscope covered or placed in its protective case when not in use.

To carry microscope:
9. Carry the microscope with one hand on the arm and the other supporting the base.
VI. Trouble shooting
Problem Probable cause Corrective action
FOCUSING
Won't focus on 40x or higher Specimen slide upside down Turn glass slide over so that the
magnification coverslip faces up
Nonparfocal Set-up procedure not correctly followed Repeat “Operation” steps 11-17
Objective has come partially unscrewed Tighten objective

LIGHTING
No light Microscope not plugged in Plug into outlet
Brightness control dial turned off Turn up light intensity
Objective not clicked into position Click objective into place

Condenser image completely off center when field Adjust image with centering screws
diaphragm is closed down
Bulb burned out Replace bulb

Bulb not inserted properly Insert bulb correctly

Fuse blown Replace fuse

Fuse not inserted properly Insert fuse correctly

No power from wall outlet Test or try different outlet

Insufficient light Brightness control dial set too low Increase brightness
Condenser diaphragm closed Open condenser diaphragm

Substage condenser lowered too far (not correctly Adjust height of substage condenser
focused) as per Koehler Method
Flickering Loose power connection Plug in microscope or try
another wall outlet
Corrosion on bulb pins Clean pins on bulb
Defective bulb socket Replace socket

Bulb not inserted properly Insert bulb correctly

Situation not covered above Call for repair

Too bright Light turned up too high Adjust brightness

MISCELLANEOUS
Eyestrain Illumination of field too intense Reduce light intensity

Eyepieces not focused for each eye Repeat “Operation” steps 11-17

Floating spots “Debris” in the vitreous humor of the retina; Taking a break & resting will often help
noticeable at high magnifications

Field diaphragm noncenterable Substage condenser seated improperly in condenser Loosen condenser clamp screw and
carrier reseat condenser
Bubbles or dark wave passes Air bubbles in oil; contact between oil immersion Clean slide and/or add more oil
across the field of view when objective and oil “broken”
using immersion oil
Designed and Developed by:
Cheryl Thayer Tryon
Barbara Smith Michael

Technical Consultants, Nikon Inc.

Mel Brenner
Barbara Loechner
KR-7221.A

MICROSCOPE LABOPHOT-2

REPAIR PARTS LIST

修理部品表

NIKON CORPORATION
Tokyo, Japan

(90.2.210)H
| Explanation of Parts List

HDA13310- “Product code No.

- 部品ーコド Parts code No.

展開図の部品につける見出し番号 index No. in the Explosion Drawing

皿 . 部品 ( 組品) の注文ーー… 3 How to order part(s)

部品 ( 組品 )は「製品コード」と「部品番」-号 art(s) should be ordered clearly indicating
を併記して注文する。 both “Product code No.” and “Parts No..”
製品コード Product code No. 部品番号 Parts No.
HDA13310 : 2K670—002
e HDA13310 i 2B500—002
HDA13310 H AL3A

РЕ e Explanation of symbols
1. 展開図の中の記号 ymbols in the Explosion Drawing
AÇ =
Lub ( )
5—5-1. -旧品5 から、互換性のない新品 5-1 に index No.5 was changed to No.5-1 which is not
変更されたが旧品 5 の要求もでき interchangeable part, but shall be supplied if needed.
meジー旧品 5 は要求できをない …- ーー -Index No.5 was replaced with modified
No.5-1 and shall be no longer supplied.
- 部表
品の中の記号 ymbols in the Parts List
olumn of Index No.
- 金属部の品組品 ubassembly, composed of metal elements
-レンズ、プリズムの貼り合わせ組品 - ptical subassembly, cemented each other
金属部品とレン、ズプリズムの組品 - ‘Subassembly, composed of metal and optical
elements
互換性のなくなった部品 ( 組品) の-…… Appended to the Index No. of new part(s) which
補助番号につける。数字は変更回数 is(are) no more interchangeable.
The number means the time of modification.
Column of Remarks
eference No.of Technical Data sheets

9.6
・その他の記号
Rev. E
変更
した箇所につける --“To be marked when part is modified.

(%)
||
內容說明 Introduction

1. 展開
図の見方 Explanation of Explosion drawing

分類 Ra EXPHRECSAUER 記號
Classification Sample drawing car [Mack
点線
で囲まれていないもの
>| AL3A
Part(%), not encircled
by a dotted line
修
o
m
部
品

A
点線
で囲まれていて、且、個々
いに補助番号がつい
ていないもの

Part (3), encircled by 2 dotted

parts

line, but without each Index No. E

as repair

AL2
regarded

点線で囲まれていて、且、個々
506D-
に補助番号がついているもの
AL
とちら
でも注文でる| 〇へ
Part(s), encircled by a dotted
Either Index No.506D,A
L 2)
line and with each Index No. will be 0.K.

(x) 印がつい
ているもの
Part(s), marked (x) をなし (注文
できない)
x
Not considered as
| repair part(s)
|
=
E 点線で囲まれていなくて、補助
Е | 天史にの曲がっ
いているもの一一 2 同
Fe S| Part(s),not encircled by a dotted =
品 &| line but with Index No. marked [O] ©
a
vw si
Si で囲まれていて、且、個々
5 | 助番号がついていないもの回
$ Part( s), encircled by a dotted
53 line, but without each Index No.
A3

補助番号 : 展開
図の中の部品 ( 組 )品の見出し番号 ……AL3A,108,L7,A2、506D など
Index No.: Number、used in stead of Part(s) No.for the sake of convenience

C4)
w
コード桁数 (Code Fig.)
機械標準品 (Standard mechanical elements ) 部品ーコド (Code No.) 一
2H Hit (Code Fig)| 1 |2|3]«|s[6]7js[ 0 {io in:
品名種 я イイ| 呼び径| 長さ | 材 ВЯ EF SUYA RTE EK
Classification Type Z Length | mtl. ¡Coat | Spare | Name used in this List
Ez Plan bead ; a | | E =
HER ad 已なべ | Machine serew | | ICISEPM.4x1.4 ©
18 (E) ountersunk head
|| | i Γι ο νο
J A = M 2 А Eğ
ICISDCMI.4x 1.4 ©
Cross recessed EE isem
ο) serew Type 1 = vai Counters 3 ・ ISO AS dat
RxS | Machine screw i JCISD0CM1.4x1.4©
B
ПЕ | (Phan head ISO SoU
1| +ERRE DAL sz e | Bastine serem о |1 E JCIS © PM2.6x10 ©
3种 + = =
Es Qomiersunk head 2 у ICISD è shot
Sİ Cross recessed |B e | Beeline screw s JCISÓ CM2.6x 109)
screw Type 3 tes val (Camtersunk hesd 3 JCIS の丸きら小ねじ
#35 | Machine screw JCIS© 0CM2.6x10 ©
fa Ban bead 1 © zxby
Z | Machine screw © PM3x0.5x5
字穴付き小ねじ F A
NN = eera |) @ ㅎ 5166
3 5 | Machine screw 5 @CM3x0.5x5
essed C B 2
ce tes Gea Comers
bead | @nzsret
screw 485 | Machine screw + © OCM 6x 60
©Jruss head = y 人上?Zal 小和
E (Machine serer 4 © TM6x 60
(EZ | Bus a Θάσρνένοιυ
HER E なべ | Tapping затея Туре В] © PTBL.4x 2.5 ©
377
A UBI | | fr | Gemerek
beni つき
5タッピンちじ
Cross recessed D = 5 | 加 spping rew Tre回 2 > ©CTB1.4x2.5 ©
tapping screw
Type B-1 Diva Comersami
head | 2 Oñr577 4780
4.85 | Tapping screw TypelB] F © OCTBI.4x
2.5 ©
1
Ce | lan bead をペタッピンちじ
FAN Es
Si |?ere)
vem)! oon 20 ©
> = HUBMSM R= | Doutersunk head у のきらタッピンねじ
Cross recessed E|" | Gosse scree Tre 回| 2 Фствахю®
tapping screw | a
Type B-3 | Oval Domtersink head | 3 つえきらタッピンねじ
Hx5 | Taping screw TyreB ©0CTB2x 10 ©
(Elan bead 1 をベタッピンわねじ
Ана ыы Е А © PT2.6x4 ©
タッピンねじ 2 種 he | es EE
Cross recessed F lime sees 2 > t © CT2.6x4 ©
tapping serew Sb)
Type 2 Dva Coutersenkbesd | S У つえきらタッピンちビ
Ras | Mapping serex © OCT 6x 50 ©
== ФЕЯ ЕВЕ
十字穴き付 = 1 А @PTTB1.4x1.8@
297248 = |
BUE == D259794 ЕВЕ
Cresta © = ες È 때 | ØCTTB1.4x1.80
tapping tight =» | Oval Clounersunk head Vi A3 539794 hat
Screw Туре В-1 {== | Tleppine Mieke screw (3 Sen ne
ASS | Tm
Elan bend 1 ⑤ なベタンプタイトねにじ
十字大付き ~ Tapping Elişi screw | | A @PTTB1.4x1.8@
タップタイトねヒじ > Tre E
B 型3 种 te» Ces best 03597774 HL
cs || ne に ЕТ ScrrB1.4x1.8@
tapping tight 全 — | Gival =Countersunk head Y の丸らきE タップタイトねにじに
Screw Type B-3 Terras Digi sere | 3 @OCTTBI.4x1.8@
5| ~ 回
η
23- Fit (Code Fig.)| 1 12131 s[6]7]8 de aos pn
= 名 5 si Ί]μυα 長き| 材智和外装 | 子信番 | 当リストで用いる名移
Classification Type Z| dia. | Length | mel. | Coat | Spare | Name used in this List
Bet screw i DUR
à % | Hound point BA SR 1 x1.2
о EB | Bet screw a i EDUENDE
Slotted set screw E060 | (lone point SC 1x1.2
Bet screw 3 B 止ねおじくぼみ先
< 4% | Half point SH 6 x20
es ge 1 A ARAHBLALAR
À À | Boi HSR 3 X0.5X3
ARBRE RU EC- | Br Bea Е 1 = 付き止ねにとかのE り先
大角大
Hexagon socket |L ETR =
set screw とがり先 | (Close Point HSC 3x0.5x3
ne e 3 i 0 Y RARE UZ
くぼみ先| ar point i HSH 3x0.5x3
AMB SAM { Mexagon Sjocket _|0:3 14 Så 六角大付きボルト
Hazagön söckenbeli MA ΕΕΕΞ head 国 olt 1 214 0 DA HSB 3x0.5x 4
平行ピン a 1:0 0 平行ビン
Straight pin EL Em Bi. 16:9 이스 오이 SP 1x3
テーバビン
Tasov pla EZ | (Paper
ματςPin 2|-
110
ARO) 10
0 F |A-Y
= テーバビン
TP 1x6

Spring Bin 3İ— 1:0 0! s |B.x スプリングビピン

Snap ring
Loki
SãO 人
iz. |
ав i i
14-Y cues
Ge | p
= = 510:0 GSH)
o | Es 3 oloio| F [AY GS1.5
= i i =
= || | o lswse
ーー 11-0050
10|010бр. 3] Х 때 :
15 0:0 E as
Steel ball | D | Re
Eva ta a i i 3
| © inch size 2 i7|0i0|F S| X SB“
: E コード © : 5 σι
械品 (Standard mechanical elements )
机标准 ο İN o
2— Witt (Code Fig.)| 1 [2[s]«[s[e{:x[s] os [win] :
品名 # я 7| sua ες İn min al Fee) oz poms ak
Classification Type ig. mtl. | Coat | Spare | Name used in this List
Фаня ze |T リャート
HE-
ヘリサート y A 1 5 x | H3-Lo
Heli-sert
Wie
42% se
| Tl
|_ Ho

凡例 (Explanations)
名 # (Name) 標準部品コード (Code No.)

p TM 3x0.5x8 | 十字六付二小加 그
C4—30080BB/*E
= Ez (Length)
me (2 )|(Cross recessed | FRE (Spare)
{Е ピッチ (Piteh) me トラズ JH (Coat) BNi
2 呼び (Diameter) ma (d) (Truss head 一材料 яя
+ 5 A( Truss head Machine screw) Machine screw) (Material) (Brass)
АН ES (Cross recessed) FUE (Diameter) 3.0m0 HX (Length) 8.0m
pe JCIS®OCM La 一 L4030FCL:
ooo rr (Type 1) re come es TOR jE
Les (Length)mm (2) | (Cross recessed Type 피 ¥ fi (Spare)
MUZE (Diameter)
mn (d) RES La (Coat)Ni-Cr]
we
Le] a 5 (Oval Countersunk head (Oval Countersunk Em
Machine screw) head Machine screw) Us (Ferroalloy)
JCISねじ (JCIS screw) MUZE (Diameter) 14am RE (Length) 3.0 mm (4
KR-7221.A

— CONTENTS ——
8. MBA33510(Microscope LABOPHOT-2)
Illustration (Fig.1 一 Fig.6) … ドドドドドドドドドーーー τς
ημας οσα ος ασ πο ο ος ο ερ ος ZIT
MBA33510
KR-7221. A

Fe I
MBA33510
KR-7221. A

(120)x3

Al
=
Lub(G6053) i
103

88 (11192
8
8 109 Bias
(130)2 |
n2 144
o lluna =
È eo
5 в (иг
E e
o
|"
(14104 < С 6 (130)2
© s
А. (1312 & o

(LISA D}n o
8

114 ubtG2o7) /

Lub : Lubricant (# #4) E

Ad : Adhesive (E 著) Fig: 2
[9
、 ae) av: PV
SEE) HW) 1 때 20907 1907
Левое
woe
sız
р SEZ
SS 902
S (FÅR 1-22
SI
-
HOT ~ / 2082)
E) :
(78010) mesea ラン cs©
CU OS
ог
i Boz 102
ㅠ 1207-81 [sv] |
MBA33510
KR-7221. A

333

(3392

(335)2

(336)2
313 É
307

315
053)

316

Lub (66053)
(334h2
316
Lub : Lubricant (8 48) E
Ad : Adhesive (E 4 Fig. 4
MBA33510 KR-7221. A

9 Ques

Lub : Lubricant (# i)
Ad : Adhesive ( 38)

|
(A +
\
1005
>
425
À For 100/120V
İ MXAZOSIGLIA 250V)
L For 2207240v Fi 5
MXA 2O5I7(TIA 25OV) 9
MBA33510
KR-7221. A

501

回(503)5

1 2G301—007 Collector lens 1 5 @(GINEW)

ュレクタレンズ
2 2G552—003 Mirror 1 5 ©(64). ®(64), ©1), ®
33-
3 2G114—018 Field lens 1 5 ©
フィールドレンズ

4 2G702 一 009 Diffuser 1 5 ©

拡散板
5 2G702—010 Diffuser 1 5 ©
拡散板

102 2K611—384 Arm rear cover 1 2 @

アーム背面カバー

103 2K 406—085 Eyepiece tube receptacle 1 2 EE UM

am
103—1 2K406—085—1 Eyepiece tube receptacle 1 2 META LAZIO
MAZO
109 2K273—063 - Rack 1 2 ©.©
ラック
ui 2K078—002—1 Cylindrical roller 24 2 O(#11), @(#11), ©. ®
円筒コg

12 2K362—017 Retainer 2 2 ©(#12, ®(#12, ©, ®

リテーナ
113 2B680—095 Hexagon wrench 1 2 ©. ©
АФУ
114 2K601—793 Roller race cover 1 2 ©. ©
ローラレースカペー

USA 2K600—191 Adjusting washer n 2 OC+15A), ©C215A), ©. ©

調整ウッシ + (【=0.05 mm)
1158 2K600—192 Adjusting washer n 2 O(# 15B), @(#15B). O. ©
ASI, y+ (t= 008m)

115C 2K600 一193 Adjusting washer n 2 O(#15C), @(#15C), ©. ®

8874%+ (t=0.1lm)

112MB ¿388 (Common to 112MB)

11MB と共通 (Commonto 111MB)
IBA13500
と共通 (Common to ΜΒΑ 13500)
MBA33500 と共通 (Common to MBA33500)
MBA33510
Parts List KR-7221. A
Index No. | Part No. Name Pes,per | Reference | Remarks
885 188 名 # [16568] sens! a 考

115D 2K600—194 Adjusting washer O(+ 15D), O(+ 15D), ©, ©

HE7>7+ (1 =0.03 mm)

119 2K021—046 HSB3 x 0.5 x6 9.6

六角穴付きポルトセムス K (小形座金平付き )
120 2K021—047 HSB3 x 0.5 x8 9 8
RARE Hb 世人又区〈小形平座金付兰 )
121 2K021 一 048 HSB4x07x8 о. ©
大角穴付きポルトセムス K (小形座金平付き )
123 2K021—007 HSB6 x20 @(# 43B). @( #438) .@.@
ABA Ev 平
セムス K (小形座金付き )

125 2K625—410—1 Cover plate (Right) ©

SD 5
126 2 K625—409—1 Cover plate (Left) 6.8
飾り (DD
127 2K681—789—1 Reversed nosepiece cover ©. ©
ELENA DRŽEN
121-1 2K681 一 789 一 2 Reversed nosepiece cover ©. ®
ELENA 一 ØRE EM
128 2K115—523 Blank cap
Ez
130 2K360-098 ide rail 6.5
2 Ky—w (L=135m)
131 2K360—013 Guide rail ©(#10B), @(#10B), ©. @
34 ELM (L= 108 mm)

140 C 120030 FC ©PM2x3

つなべ小ねじ
141 C1—20030 FF @PM2x3
ex Mat

12MB¿ 368 (Common to 112MB)

142 C1—30060 FE @PM3 x 0.5 x6 3 3

⑤ なべ小ねじ
143 C1—30100 FE ©PM3 x 0.5 x 10 1 2
③なべ小ねじ
144 C2-3012 FE @CM3 x0.5 x12 1 2
Os Shae
145 L2—30080 FF HSC3x05x8 . 4 2
六角穴付き止ねとじがり先 a E E
146 MI
一03006 FF HSB3 x0.5 x6 3 3
六角穴付きルト
ボ
149 P4—20050 SX SPL2x5 7 2
スプリングビピン

201 2K115—524 Fine focus knob cap 2 3 es

微動ハンドルゴムキャップ

202 2K201—128 Fine focus knob (Left) 1 3 @

微動ハンドル (な) +В 12 =
202-1 2K201—128—1 Fine focus knob (Left) 1 3 ©.©
ab Fa (© +06 1.70
203 2K201—129 Fine focus knob (Right) 1 3 ©. ©
Alin Kr (5) +- 612
203-1 2K201—129—1 Fine focus knob (Right) 1 3 ©. ©
am +» (8) 4-617=
204 2K201—130 Coarse focus knob (Left) 1 3 0.8
粗動ハンドル (かな)
205 2K201—131 Coarse focus knob (Right) 1 3 ©. ©
Boz 05)

206 2K631—323 Fine focus thrust stopper 1 3 ©, ©

微動スラスト受け +- 12m
206-1 2K631—323—1 Fine focus thrust stopper 1 3 ©. ©
後動スラスト受け +- 17 =
207 2K150—175 Circular nut 1 3 6.8
丸ッナト
208 2K631—324 Pinion holder 1 3 ee
ビニオンホルダ
209 2K641—225 Adjustment ring supporter 1 3 ©.
トルク調リン
節グ
210 2B640—031 Torgue adjustment ring 1 3 ©.©
トルク調リ節ング
213 2K160—790 Gear supporter 1 3 6.8
ШЕЕ
215 2K631—325—2 Coarse focus shaft 1 3 ©. ®
зом
217 2K641—228 Coarse focus knob supporter 1 3 6.8
粗動ハンドル受け
218 2K014—008 &PM3 x0.5 x6 6 3 IONE)
BUNU +LxC (ARESHA)
219 2K165—263 Delrin washer 1 3 ©. ©
デルリンワッシャ
22 2K248—017 Belleville spring 1 3 6.9
さらばね
221 2K165—264 Delrin washer 1 3 6.8
デルリンワッシャ
22 2K246—134 Wave washer 1 3 ©. ©
波形座金
23 2K641—229 Friction disk 1 3 6.8
Es MIE
225 2K260—131 Gear 1 3 ©. ©
an
225 2K260—132—2 Gear 1 3 ©.©
an
227 2K260—133 Gear 1 3 ©. ©
an

(O: MBA 13500

è $i (Common to MBA13500)
© : MBA33500
$83 (Common to MBA33500)

230 28260-034 Gear 1 3 ©e

nu
22 2B260—035 Fine focus shaft 1 3
uma
232-1 2B260—035—1 | Fine focus shaft 1 3
ave
28 2K150—163 Circular nut 2 3
Atv

241 2K641—233 Cover ring 1 3

カバーリング

262 C1—30060 FE @PM3 x0.5 x6 6 3

hat
23 L3-30050 FE HSH3 x0.5 x5 4 3
大角穴付き止ねじくぼみ先

301 2K680—242-1 Substage 1 4 Common to $201 in 112MB1

サプステージ 112MB1 04201 ¿368
302 2K420—053 Stage attaching dovetail 1 4 ©(+202), @(#202), ©, ©
ステージ取付アけリ

303 2K680—196 Condenser carrier 1 4 (#203), @(#203), ©, ©

コンデンサキャリア

: 112MB
と共通 (Common to 112MB)
111 MB ¿ 348 (Common to 111MB)
МВА 13500
+ 3888 (Common to MBA 13500)
: MBA33500
č štiš (Common to MBA33500)

304 2K420 一 054 Condenser receptacle 1 4 ©(+204), ©(+204), ©, ©

FIRE

305 2K420—049 Dovetail 1 4 ©(+25). ©(+205), ©. ©

g7”
307 2K273—015 Rack 1 4 0(+207), ©(+207), ©, ©
522
308 2B264—001—1 Pinion 1 4 OCA 14), ACA 14), ©, ©
x ビニオン

310 2B201—017 Condenser focus knob 1 4 ОСА 15), ©CA 15), ©. ©

コンデン
サ上下動ハンドル
312 2K650—007 Pinion holder 1 4 O(+212), ©(+212), ©,©
ΕΞΑΥΜΣΕ

819 2K240—031 Leaf spring 1 4 ©(+213). ©(s213), ©. ©

板ばね
314 2K625—059 Spring retainer 1 4 (#214), @@214), ©. ®
ばね押え
315 2K380—058 Condenser clamp screw 1 4 O(#215), @(#215), 0. @
コンデンサクランプねじ
316 2B380-015 Condenser centering screw 2 4 O(A 16). Q(A 16), 0, &
コンデン
サ心出しねじ
317 2 K362-021-1 Stopper pin 1 4 (#217), @(217), ©. ©
制限ビン

318 2K220—051 Coil spring 1 4 O(+218), ©4218), ©. ©

コイルばね MBA Y 77 ER
KC045—035—0100
319 2K165—077—1 Delrin washer 2 4 (219). @(#219), ©. ©
デルリンワッシャ

320 2K246—041 Wave washer 1 4 O(:22). @ (422), ©. &

波形座金
325 2K420—050 Dovetail groove (Left) 1 4 O(#206A), @(#206A), ©,®
a7) 左)
326 2K420—051 Dovetail groove (Right) 1 4 O(#206B), @(#206B), ©,@
87) (B)

O: 112MB¿ 344 (Common to 112MB)

O: 111MBと共通 (Commpn to 111MB)
(O): MBA13500 ¿ 348 (Common to MBA13500)
©: MBA33500 ¿348 (Common to MBA33500)

331 B2 一 20050 FF JCIS@CM2x5 © 2 4

ICIS@S Sav
332 2K003—019 @PM2 x3 1 4 ©. ©
Θα

333 C2-—30060 FF @CM3 x 0.5 x6 1 4

Gs oar
334 C1—30060 FF ©PM3x0.5x6 4 4
Oral
335 C1—30080 FF @PM3 x05 x8 4 4
ETATS
336 2K014—008 @PM3 x0.5 x6 2 4 (#234), @(#234D). ©, ®
Buat *L2C AMBSHE)
337 M1—04012 FE HSB4 x0.7 x 12 4 2
AAS tb
338 MI
一 04014 FE HSB4x07x14 3 4
ARH de +
339 L3—30050 FF HSH3 x 0.5 x3 5 4
ARAM EAL (SH

402 2K611 一 371 一 1 Base upper cover 1 5 ©

ベュスギカバベニ

403 2K501-020-1 Field lens holder 1 5 6.8

フィルドレンズホルダ

407 2B572—001 Blade guide way 1 5 ©. ©

RE
408 2K574—042 Blade mount 1 5 6.8
вом
409 2B267—002—1 Field diaphragm control ring 1 5 © ©
BR AY 7

©: MBA13500
+ 3t8i (Common to MBA 13500)
© : MBA33500 と共通 Common to MBA33500)

一 13 一
MBA 33510
Parts List KR-7221. B
Index No. Part No. Name Pcs.per | Reference i Remarks
Unit | Fig.No.
補助番号部品番号名称 16958M| PRIZE | a *

411 2K240—264 Field lens retainer 1 5 6.2

フィルドレンズ押え ιά

412 2K475 一 049 Field diaphragm scale 5

視野絞り sae
413 2K087 一 698 Bottom plate 1 5
底板
414 2K611—372—1 Lamp socket holder —— q 5 ©
ランプソケットホネルダ

415 2K681—776 Lamp house cover 1 5 ©

ランプハウスカバベー

416 2K611—373 Heat resisting plate 1 5 ©

ENE
417 2K087—665—1 Switch panel 1 5 ©
スイッチバネル

419 * 2K110—427 Rubber foot e 4 „s 6.9

了人足

420 2K380 一 233 Adjustable foot 1 5 640.8

ㅅ - ㅅ 조 페츠 토모
421 2K601—782 Blank cap 1 5
πα
422 2K083—316 Voltage select switch cover 1 5 IONE)
電圧切換え表示銘板
424 2K681—772 Brightness control knob — 1 5 ©. ©
調つ光まみ
425 2K681—777 Filter cassette 1 5 ©
フィルタカセット

426 25652-009 Halogen lamp < 1 5 ©

ハロゲンランプ (6V 30W) Delivered as a product
営業販売品

⑯: MBA13500
と共通 (Common to MBA13500)
@ : MBA33500
と共通 (Common to MBA33500)

May 1990 x1 Revised

改正

431 B1—20060 FE JCIS©PM2x6 © 4 5

JCIS③ なべ小ねじ
432 B1—20080 FF JCIS@PM2x8 © 3 5
JCIS@ RL
433 C1—30060 FE @PM3 x05 x6 18 5
③ なべ小ねじ
434 2K014—001 ©PM3x0.5x6 3 5 @(*451A)@(*41F)@(449)@
つなべ小ねじ i KAZA MINERA),
435 2K014—027 ©PM4 x0.7x6 1 5 ©4890 |
②③ なべ小ねじそムス A (ばおね座金付き )
436 C2—30060 FE @CM3 x0.5 x6 5 5
Ss Shae :
437 B1—20050 FE JCIS@PM2x5 © 2 5
JCIS@ 丰 -<小
438 F1—26140 FA ©PT26x14 © 12 5
なベタッピンねじ
439 2K021—037 HSB3 x0.5 x8 4 5 642,8
ABRÍAN A モムス K (小形
平座金付き)
440 P3—30060 SX SPN3 x6 1 5
スプリングビン

41 R1—02000 FE PWS2 6 5
TRONCA
442 C1—20120 FC @PM2 x12 1 5
⑤ なべ小ねじ

: 112MB
と共通 (Comrmon to 112MB)
: 111MB
と共通 (Common to 111MB)
: MBA13500
と共通 (Common to MBA13500)
: MBA33500
と共通 (Common to MBA33500)

501 2B001—120—1 Nosepiece ei)

レポルバ

501A 2K080—031 Cover plate SGA)

BOR
501B 28240-008 Click spring (3018)
クリックばね
501C 2K640—783 Revolving ring @(301C〉
レポ
ルババ回転環

501D A2—20025 FC JCIS©CM2x25 ©

ICIS@s Shae
501E B1—26045 FA JCIS@PM26 x45
JCIS のなべ小ねじ
502 2K576—233—1 Circular nut Common to 302 in MBP31102
Aşık MBP31102
の 302と共通
503 2K467—003 jiece cap O da
レポルズyeバ用対物キャップ ¡vered
FRERE as a prod

1001 A 2S418—018 AC inlet ©. ©

AC インレット şana O
1001 B 25423-028 See- saw switch 6.8
シーソースイッチマッカート 1852-0122
1001 C 25400—667 Socket housing ON)
ソケットハウジング日本圧着適子 S2P-SVF 2 極
1001D 25400-669 Socket contact me EİN
ソケットコンタクト DE >

1002A 28322-137 Slide variable resistor LIMA, ®(1005A)

254ライド FERS HER VJ4513—4NI5C-10A10
RAT AC
1002B 2 S424 一 032 Slide switch 2979 S-1 UL
スライドスイッチ

@: MBA 13500
¢ 3t@ (Common to MBA13500)
© : MBA33500
č 3t (Common to MBA33500)
©: MBA31103
¿ 368 (Common to MBA31103)

— 16
MBA33510
Parts List KR-7221. A
Index No. | Part No. Name Pes,per Remarks
Unit i
補助番号部品番号名 $ 1 台分個数 | sans | A =

1002C 25400-668 Socket housing 1 BEET S6P-SVF 635

ツケットハウジング
1002D 25400-669 Socket contact 5 Quo). SCD D), a
5 トコントー01Tー1.
R 1001
Dと共通
1003 25040-578 Lamp socket with cable ——— | ©
ケーブル付きランプソケット
1005 25463-036 Fuse holder 1 ©1006), & (1006)
モューズホルダ πα ΜΕΣ
1007 28740-047 Cable clamp 4 (1009). ®(1009) *
ケーブルクランプ北川工業 NK-5N の84
1008 2S740 一 063 Cable clamp 1 @ 1010)
ケーブルクランプ AIR КСА- の43

1101 2S001—034—1 Brightness controller 1 る аа

mon in
AV O MCA 141000
1201 と共通

A7 2B260—036 Gear | — 1 6.8

ag
A9 2B570—022 Blade 一一 10 한은 to 121 in MAA15500
絞り羽根 MAA15500
の 121 と共通

@: MBA13500
と共通 (Common to MBA13500)
© : MBA33500
と共通 (Common to MBA33500)

=17 =
KR-7263.A

STAGES
FOR
MICROSCOPE OPTIPHOT-2, LABOPHOT-2
題微鏡OPTIPHOT-2, LABOPHOT-2 ステ
用ージ

REPAIR PARTS LIST

修理部品表

NIKON CORPORATION
Tokyo. Japan

(91.9.210)H
[KR-7263A|

— CONTENTS —

$. MBC21102 (Rectangular Mechanical Stage “R2”)

Illustration (Fig.1—Fig.2)
Parts List
ΚΗ- 12063 Α

(X) Upper plate

Lub : Lubricant (i)

Ad : Adhesive (№ 8) Fig
MBC21102
KR-72 63. А

104

P ESE

9 (107)x2

101

Lub(

= 109A„C

Lub : Lubricant ‘#8 #

Ad : Adhesive JE É μια. 2
MBC21102
Parts List KR-7263. A

Index No. Part No. Name Pcs.per Reference. Remarks

Unit Fig.No.
補助香號 ERE z # 16984 ERE m

5 2K078—003—1 Cylindrical roller 16 1 ©. ©. ©. © (6)

円筒ロコ (D= 3175 mm)
6 2K 347—005 Retainer 4 1 ©. ©. 9. ® (7)
i リテーナ
7 2K 360-005 Guide rail 8 1 ©. ©. @. ® (73)
ガイドレール (L 126 mm )
10 2K 273-035 一 2 Rack (Lateral) 1 1 ©. ©
747 ESR)
1 2K 360-006 Guide rail 4 1 ©, ©. 9. 5 (14)
ガイドレール (Lニ= 174 mm)
12 2K 360-007 Guide rail 4 1 O, O, 6, 9 (15)
ži4 Fy—w (L= 184 mm)
13 2K 240-105 Leaf spring 2 1 ©. ©. ©. © (45)
板ばね
16 2K 470-079 Vernier (Lateral) 1 т о. ©. ®
HR (ESR)
17 2K 470-016 Scale (Lateral) 1 1 ©. ©. 9. © (28)
ER (ESR)
18 2K 470-050 Scale (Longitudinal) 1 1 O, O, e
主尺 (前後用 )
19 2K273-051-3 Rack (Longitudinal
)一一 1 1 о. ©
379 (前後用 ) >
20 2K 680-021 —2 Vernier mount E 1 1 O. O, 9. ® GD
HIRE
21 2K 058-004 Toothed washer 2 1 ©. ©. @, ® (66)
歯付き座金 2一
22 2K 470-002 Vernier (Longitudinal) 1 1 ©. ©. 9. © GD
副尺 (前後用 )
28 2K600—071—1 Stopper 1 1 ©. ©. @. ® (26)
ストッパ
24 2K600-617-1 Spacer 1 1 ©. ©, ©. © (4-1)
Z スペーサ
25 2B380—010—1 Stage clamp screw 1 1 ©, ©, © (A5-D
ステージクランプねじ

: MBC21100 と共通 (Common to MBC21100)

@@@ 〇

: MBC21103 と共通 (Common to MBC21103)

: MBC 21000 と共通 (Common to MBC21000)
: MBC11101
と共通 (Common to MBC11101)

24
MBC 21102
Parts List KR-7263. A
Index No. Part No. Name Pes.per Reference Remarks
R Unit Fig.No.
补助香号部品番号る称 1 BR SERE m 3

31 A1- 17040FC JCIS © PM17x4 © 4 1

JCIS ⑤ な <べ小ねじ

32 A1-20040FC 。 JCIS © PM2x4 © 2 1

ICIS @ な <べ小ねじ
38 B1—2040FC JCIS © PM2x4 © 2 1
1015 © a ptr
4 B1—20050FC JCIS © PM2x5 © 2 1
1015 〇なべ小ねじ
35 M1—03006 FF HSB3x0.5x6 2 1
KAR
付きボルト
36 M1—03010FF HSB3x0.5x 10 4 1
AMARE KK
37 M1— 04008 FF HSB4x07x8 6 1
六角穴付きボルト
38 P3—20050SX SPN 2x5 18 1
スプリングビピン
39 P3-30100SX SPN 3x10 2 1
スプリングビピン
40 R1-02000FY PWS2 1 1
平座金小形丸
41 MI 一 04012FF HSB 4x07x12 2 1
a 六角穴付きポルト
43 2K 160-276 Washer 2 1 O, O, e
座金
4 R1—04000FF PWS 4 2 1
平座金小形丸

[BC 21100 ¿38 (Common to MBC21100)

©: MBC21103 と共通 (Common to MBC21103)
@ : MBC21000 と共通 (Common to MBC21000)
MBC 21102
Parts List KR-7263. A
Index No. | Part No. Name Pes,per Reference ; Remarks
| Unit Fi .
apes | unes = 15384. SARE a =

51 2B 201-001 Knob (Longitudinal) 1 1 ©, ©. © (3)

POD)
52 2B 201-002 Knob (Lateral) 1 1 ©. ©. © (14)
ハンドル (左右用 )
53 2K 340—006—1 Pinion cover 1 1 O, O, © (61), © (9)
ピニオンカバー

54 , 2K 630—005—1 Pinion holder 1 1 о. ©

ビニオン軸受
55 2K264-010-1 Pinion (Lateral)一 1 1 о. ©
已= 才 > (左右用)
56 2K 264-041 Pinion (Longitudinal ) > 1 1 о. ©
ビニオン (前後用 )
57 2K 156—020 Nut 1 1 O. O. 8 15)
F

58 2K 156-021 Nut 1 1 ©. ©. © (16)

toh

59 2K 246-058 Wave washer 1 1 ©. ©. & U7NEW)

波形說金
60 2K 246 一 059 Wave washer 1 1 ©. ©. © a8NEW)
波形說金
61 2K 165—050 Delrin washer 2 1 O, O, 8 (19)
デルリンワッシャ

62 2K 165-051 Delrin washer 1 1 O, O, @ (63). @ (20)

デルリンワッシャ

・ 3 2K 165-061 Delrin washer 3 1 ©. ©, © (64), ® (21)

デルリンワッシャ

68 N1- 04000FY HN4 © 1 1

六角ナット
69 及 1 一 04000FY PWS 4 1 1
平座金小形丸
70 R1—04000BX PWS 4 i 1
平座金小形丸

@ : MBC 21100 上共通 (Common to MBC21100)

© MBC 21103 上共通 (Common to MBC21103)
e MBC 21000 と共通 (Common to MBC21000)
© : MBC11101 と共通 (Common to MBC11101)
W
MBC 21102
Parts List KR-7263. A
Index No. Part No. Name Pes,per Reference Remarks
Unit Fig.No-
福助番号 | 部品番号名 π 1678% SEDE a =

101 2K625—167—1 Specimen holder 1 2 © (2-1)

Barry
102 2B625-011 Specimen clip 1 2 © (A7)
標本押え >
104 2K 370-057 Specimen clip thrust metal 1 2 ©. ©. © (35)
標本押えスラスト受け
105 2K 230-003 Spiral spring ~ 1 2 ©, © 66)
渦巻ばね
106A 2K600—072—1 Adjusting washer n 2 O, ©. © G7A)
調整ワッシャ (t =04mm)
106B 2K600—073—1 Adjusting washer n 2 ©. ©. © G7B)
調整ウッシャ (t= ニ 05mm)
107 2К 380—033 Specimen holder clamp screw 2 2 ©. ©. © (39)
βαν Έεου
108A 2K 600-074 Adjusting washer n 2 ©, @, @ (0A)
調整ワッシャ (も t ニ03mm)
108B 2K 600-075 Adjusting washer 2 2 O, ©. © (40B)
調整ワッシャ (t =0.4mm)
108C 2K 600-076 Adjusting washer n 2 ©. ©. © aoc)
調整ワッシャ (t=0.5mm)
108D 2K 600-077 Adjusting washer n 2 ©. ©. © (40D)
調整ワッシャ (t =06mm)
109A 2K 600-078 Adjusting washer n 2 ° ©, @ @ GIA)
調整ワッシャ (t =02mm)
109B 2K 600-079 Adjusting washer n 2 * ©, @ @ GIB)
MET >7+ (t =03mm)
109C 2K 600-080 Adjusting washer 2 2 ο. 9. 9 (10)
調整ワッシャ (t =0.4mm)

115 B2—20040FC JCIS @ CM2x4 © 2 2

JCIS © #3/hal

116 2K 003-004 @ CM 3x05x5 1 ο ® (64)

のさら小ねじ

@ : MBC 21100 と共通 (Common to MBC 21100)

Al Al Specimen clip supporter 1 2 ©. © (A6R)

標本押え保持金物
A2 A2 Leaf spring retainer (stopper) 2 1 ©. ©. ©. © (A8)
板ばね押え
A3 A3 Roller race | — 1 1
ローラレース

A4 A4 Roller race < ^ 1 1 ©. ©

ローラレース

A5 A5 Roller race 7 1 1 o, e. e
ローラレース

A6 A6 Roller race 1 1 o. e. e
ローラレース E

@ : MBC 21100 と共通 (Common to MBC21100)

@ : MBC211と03
共通 (Common to MBC21103)
@ : MBC210と00
共通 (Common to MBC21000)
@ : MBC 11101 と共通 (Common to MBC11101)
いっ
W
Nikon

Biological Microscope

LABOPHOT (Y-R stand)

INSTRUCTIONS

NIPPON KOGAKU K.K.

CAUTIONS

o Avoid sharp knocks!

Handle the microscope gently, taking care
© Focus knobs
Never attempt to adjust the tightness of
to avoid sharp knocks. the right- and lefthand focus knob by turn-
ing the one, while holding the other in this
o When carrying the microscope
When carrying the microscope, hold its arm
model
disorder,
microscope, because of causing

with one hand, supporting the bottom of

the microscope base with the other. The
instrument weighs about 8 kg.

© Place for using

Avoid the use of the microscope in a dusty
place, where it is subject to vibrations or
exposed to high temperatures, moisture or
direct sunlight.

Light source
©

Use halogen lamp 6V — 20W.

In lighting the lamp

Take care not to touch the rear cover of

the lamp being lighted, and don't bring
inflammable substances such as gasoline,
thinner, and alcohol near to the cover, as it
may take a high temperature while the
lamp is being lighted.

© Exchanging the lamp bulb and fuse

Before replacing the lamp bulb
(6V — 20W)
or fuse, turn OFF the power switch and
disconnect the plug of the power source
cord.
In such cases as of replacement, do_not
touch the lamp bulb with bare hands,
immediately after putting out the lamp.

Dirt on the lens

Do not leave dust, dirt or finger marks on
the lens surfaces. They will prevent you
from clear observation of the specimen
image.
CARE AND MAINTENANCE CONTENTS

I. NOMENCLATURE
о Cleaning the lenses
To clean the lens surfaces, remove dust II. ASSEMBLY
using a soft brush or gauze. Only for ill. MICROSCOPY
removing finger marks or grease, should ~ 1. Operating Procedure ............
soft cotton cloth, lens tissue or gauze 2. Manipulation of Each Element .....
lightly moistened with absolute alcohol 1) Interpupillary distance
(ethanol or methanol) be used. adjustment ................
For cleaning the objectives and immersion 2) Diopter adjustment
oil use only xylene. 3) Optical path change-over in the
For cleaning the surface of the entrance trinocular eyepiece tube . .......
lens of the eyepiece tube and the prism 4) Centering the condenser lens. ....
surface of the Trinocular Eyepiece Tube 5) Use of condenser aperture
"T" or the Ultra Wide Eyepiece Tube diaphragm
“UW”, use absolute alcohol. 6) Use of field diaphragm. ........
Observe sufficient caution in handling 7) Focusing
alcohol and xylene.
IV. OPTICAL SYSTEM

o Cleaning the painted surfaces V. PHOTOMICROGRAPHY

Avoid the use of any organic solvent (for VI. USE OF THE ACCESSORIES
example, thinner, ether, alcohol, xylene
VII. TROUBLE SHOOTING TABLE ......
etc.) for cleaning the painted surfaces and 1. Optical
plastic parts of the instrument.
2. Manipulation
3. Electrical
© Never attempt to dismantle ! 4. Photomicrography .............
Never attempt to dismantle the instrument
so as to avoid the possibility of impairing ELECTRIC SPECIFICATIONS
the operational efficiency and accuracy.

a When not in use

When not in use, cover the instrument with
the accessory vinyl cover, and store it in a
place free from moisture and fungus.
It is especially recommended that the
objectives and eyepieces be kept in an air-
tight container containing desiccant.

© Periodical checking
To maintain the performance of the instru-
ment, we recommend to check the instru-
ment periodically. (For details of this
check, contact our agency.)
I. NOMENCLATURE

CF eyepiece

Eyeguard

Diopter ring

Interpupillary
distance scale

R-revolving nosepiece

CF objective

Stage clamp screw

Condenser aperture diaphragm
control ring

Condenser clamp screw

Filter receptacle

-axi b É E
Stage Y-axis travel kno Brightness control dial
(Including power switch)

Stage X-axis travel knob Condenser focus knob

Daylight filter Dust cap

Fig. 1
Binocular eyepiece tube

Eyepiece tube clamp screw

Slide holder Coarse focus knob

Condenser Fine focus knob

Condenser centering screw

Lamp socket

Field lens

Field diaphragm control ring Fuse holder

Power cord

Fig. 2
II. ASSEMBLY
© To assemble the microscope, follow the procedure in the order given:

(D Leveling foot screw

For stable installation of the
microscope, manipulate the adjust-
ing screw at one foot on the bot-
tom of the microscope base.

rep
y

(2 Lamp bulb
Insert the lamp bulb with its
Г
|
|
pins into the accepting hole
in the socket. Condenser
Note: Don't touch the bulb
surface directly with the
clamp screw
fingers.

Power cord

(3) Lamp socket Y-R stand

Insert the socket into the
receptacle on the micro-
scope base.

Dos: filter
Place the filter on the field
lens. 45 mm in diameter.
(5) Eyepiece
Insert the eyepieces into the eye-
piece sleeves of the eyepiece tube.

È (4) Eyepiece tube

After releasing the eyepiece tube clamp screw
sufficiently on the right side of the microscope
arm, attach the eyepiece tube, and clamp it in
position.

(の Objectives
Take stage down by manipulating coarse focus
knob. Mount the objectives on the revolving
nosepiece in such positions that, when, viewed
from the stage-side, their magnifying power in-
creases clockwise.

Slide holder
If the microscope is to be used with the lefthand
stage travel knobs, attach the slide holder to the
righthand hole, and if with the righthand knobs, to
ser the lefthand hole. Slide holder is to be removed
when using large specimen.
reW

=— Stage clamp screw

Releasing the stage clamp screw suffi-

ciently, fit the stage into the circular
dovetail, and refasten the clamp screw.

Aperture number plate

Condenser
Insert the condenser into the condenser carrier,
facing the aperture number plate toward the
user, and fasten the clamp screw.

Fig. 3
Ill. MICROSCOPY
1. Operating Procedure
1) Turn the brightness control dial (including power
switch) to ON and set the scale on the dial to 4.
2) Remove the dust cap and place the daylight filter
onto the field lens.
3) Place the specimen on the stage and swing the
10X objective into position. Focus on specimen.
4) Adjust the interpupillary distance and diopter.
(Refer to P. 9)
5) Carry out the centering procedure for the con-
denser. (Refer to P. 10)
6) Swing in the objective to be used and refocus on
specimen.
7) Adjust the condenser. (Refer to Table 1)

Table 1. Use of Condensers

Type of Abbe Swing-out Achromat Achromat/aplanat
condenser condenser condenser condenser
Object N.A. = 1.25 N.A. = 0.9 N.A. = 1.35
distance Dry system Oil-immersion
Objective’ 2mm 1.8mm 1.6mm
1x Remove the Remove the condenser
※2X condenser Remove the
※4x Top lens swung out condenser

10x
20x
Usable Top lens swung in
40x Usable
100x

[NOTE] © The above object distance (from the top of the

condenser lens to the specimen surface) includes a
glass slide thickness 1.2mm.
*e When using the Swing-out condenser with 2X or
4X objective, fully open its aperture diaphragm.
© UW (ultra-wide) viewfield observation is possible
with 2x © 100X objective. In combination with
the Abbe condenser, however, the use of the 10X
or higher ob jective is possible.
© For photomicrography using the 4X or lower ob-
jective, remove the Abbe condenser.
© For photomicrography using the 2X objective,
preferably remove the condenser.
© For observation with the 1X objective, additional-
ly use the diffuser. (available on order).
© The Achromat/aplanat condenser is not included
in the standard set.

8) Brightness is adjusted by changing the lamp

voltage.
9) Adjust the condenser aperture diaphragm and the
field diaphragm. (Refer to P. 10, 11)
2. Manipulation of Each Element © Repeat the above procedure two times, and
a perfect diopter adjustment will be achiev-
1) Interpupillary distance adjustment
ed.
Place a specimen on the stage, and focus on the
© The above adjustment, compensating the
specimen. As shown in Fig. 4, adjust the inter-
diopter difference between the user's right
pupillary distance, so that both the right and
and left eyes, will keep the tube length of
left viewfields become one.
microscope correct, thus enabling him to
realize the full advantages of the highclass
objectives, including their parfocality.
(4) Since the CF eyepieces are of high eyepoint
type, it is not necessary for the user putting
on his spectacles to remove them.
Only fold down the eyeguard rubber.
(Fig. 7)

Fig. 4

2) Diopter adjustment
Make diopter adjustment for both the right and
lefthand eyepieces.
(1) Turn the diopter ring on each eyepiece,
until the end surface of the milled ring
coincides with the engraved line, as shown
in Fig. 5. Fig. 6 Fig. 7

3) Optical path change-over in the trinocular

eyepiece tube
Diopter ring
(1) When using the trinocular eyepiece tube
“pr
End surface
As shown in Fig. 8, when the observation
11111888188118811 tube is turned toward the user, 100% of
Engraved line
light enters the observation tube.

Observation
tube 100%
Fig. 5

Mount the specimen on the stage. Swing

the objective 40X into position, and bring
the specimen image into focus. For facilitat-
ing the focusing, first use the 10X and then
40X objective.
(3) Thereupon, swing the objective 4X into
position. Fig. 8
Without manipulating the coarse and fine
As shown in Fig. 9, when the observation
focus knob, turn the diopter rings on the
tube is revolved 60° leftward, 100% of light
eyepieces, so that the specimen images in
enters the vertical photo tube.
the right and lefthand eyepieces are focus-
In either case, turn the tube to the limit.
ed individually.
denser vertically so that a sharp image of
Vertical photo I
tube 100% | the field diaphragm is formed on the speci-
men surface.
(2) Bring the field diaphragm image to the
center of the field of view by means of the
condenser centering screws. (Fig. 12-[1))
Change over to the 40X objective, and
adjust the field diaphragm so that the
image of the diaphragm is about the same
as that of the field of view, as shown in Fig.
Fig. 9 12-12). If not centered, use the condenser
centering screws again.
(2) When using the trinocular eyepiece tube
"T" or the ultra wide eyepiece tube
UW"
Image of field
As shown in Fig. 10, with the change-over diaphragm
knob pushed in, 100% of light enters the
observation tube.
— Eyepiece
Observation 還 viewfield stop
tube 100%

NT Image of field.
— Optical path 、 diaphragm
change-over
knob

Eyepiece
グ a viewfield stop

Fig. 10
Fig. 12
As shown in Fig. 11, with the change-over
knob drawn out, the proportion of light 5) Use of condenser aperture diaphragm
entering the binocular observation tube The condenser aperture diaphragm is provided
and vertical photo tube will be 14 : 86. for adjusting the numerical aperture (N.A.) of
the illuminating system of microscope. It is
Observation tube:
vertical photo important because it determines the resolution,
tube = 14:86
contrast and depth of focus.
In general, when it is stopped down to 70 ~
80% of the numerical aperture of the objective,
change-over
knob a good image of appropriate contrast will be
obtained. (Fig. 13)

Fig. 11

4) Centering the condenser lens

(1) Close the field diaphragm in the microscope Objective pupil

base to its smallest size by means of the

Aperture diaphragm |
field diaphragm control ring. Rotate the Size of the condenser aperture diaphragm
condenser focus knob to move the con-
Fig. 13

10
The graduation on the Abbe condenser indicates the stage 4.7mm.
the diameters in mm of the aperture diaphragm The range of coarse and fine motion is within
opening of condenser. 30mm; 2mm up and 28mm down from the
After removing the eyepiece from the eyepiece standard position,
tube, adjust the size of the diaphragm, observ- Tightness of the coarse-fine focus knob having
ing the image of the diaphragm which is visible been properly adjusted by the manufacturer,
on the bright circle of exit pupil of objective it should never be readjusted in this model
inside. microscope by turning the one knob while
It is recommended to take note of the diameter holding the other.
of the diaphragm opening for each objective
power, whereby the best image is obtained.
The Swing-out Achromat and Achromat/
aplanat condensers, however, have a graduation
indicating the numerical apertures (N.A.), and
not the diameters of diaphragm opening.
Manipulation of these condensers is the same as
that of the Abbe condenser. Stopping down the
aperture diaphragm too far will deteriorate the
image quality of microscope due to diffraction
of light. Therefore, it is not recommended to
stop down the aperture to a size smaller than
60% of the N.A. of the objective in use except
when observing almost transparent specimen.
6) Use of field diaphragm
The field diaphragm is used for determining the
illuminated area on the specimen surface in
relation to the field of view of the microscope.
Generally, it is stopped down to such an extent
that the circumference of the illuminated area
circumscribes or inscribes that of the eyepiece
field of view. If the former be larger than the
latter, extraneous light will enter the field of
view, causing flare in the image and lowering
the contrast. Therefore, especially in photo-
micrography, the proper adjustment of the field
diaphragm is very important. Generally, good
results will be achieved when the diaphragm is
stopped down to such an extent that the dia-
meter of illuminated area is slightly larger than
the diagonal of film format.

7) Focusing
The relation between the direction of rotation
of the focus knobs and that of vertical move-
ment of the stage is as indicated in Fig. 14.
One rotation of the fine focus knob moves the
stage 0.2mm.
The graduation on this focus knob is divided
into 2um.
One rotation of the coarse focus knob moves

11
IV.OPTICAL SYSTEM
The CF objectives and CF eyepieces adopted in of viewfield, the objectives provide an
the Nikon Biological Microscope LABOPHOT unsurpassable high resolution and contrast
are designed on the basis of a new Nikon- of image over a wider field.
developed concept “Chromatic Aberration Focusing at the center means simultaneous
Free” With the Nikon CF optical system the focusing at the marginal part of viewfield.
chromatic difference of magnification in the They are excellent for ultra-wide observa-
objective and eyepiece is individually corrected. tion and photomicrography.
This is unlike conventional microscopes where (3) Plan Apochromat (CF Plan Apo)
the corrections of such aberration has been, for The use of fluorite and special, low color
the most part, compensated for in the objectives dispersion optical glasses improves the
and eyepiece as a pair. As a result the Nikon correction of chromatic aberrations over
Microscope LABOPHOT has no orange colored the entire visible region up to the line g
fringe in the eyepiece. In cooperation with the (violet) along with the lines C and F.
other optimum aberration corrections such as These highest-grade objectives with their
the Nikon Integrated Coating, a uniformly large numerical apertures produce an ideal
sharp image, much superior in resolution, image over a wide viewfield. With their
contrast and color rendition is achieved over outstanding definition, superior color re-
100% of the effective, even, super-wide field of producibility, and prominent image flat-
view, for observation as well as color photo- ness, they are especially suited for most
micrography. profound study of minute structures and
color photomicrography.
1. Objectives (4) Epi-fluorescence (CF UV-F)
Mechanical tube length of 160mm and parfocal Exclusively designed for episcopic, fluo-
distance of 45mm (This is longer than the rescence observation, this type objectives
33.6mm of earlier microscopes). In every case use non-fluorescent and non-solarisation
use the CF objectives in combination with the materials and a strictly chosen cementing
CFeyepieces. agent, to increase the transmission of UV
1) Types of objective exciting light (ultra-violet rays). Special
(1) Achromat (CF) weight being attached to the correction at
In this type of objective, the correction of the center of viewfield, and the numerical
chromatic aberrations is based on the lines apertures made extremely large, they
C (red) and F (blue). Importance being ensure bright and sharp fluorescence images
given to the correction at the center of using every excitation method. As im-
viewfield, the objectives offer the finest mersion fluid, the objectives 10X < 100X
definition and highest contrast of image of this type require the use of non-fluo-
at the center. Even the 40X and 100X rescent glycerine of high purity. -
objectives fulfill the “Chromatic Aberra- 2) Use of the objective
tion Free” correction, which has been (1) “Oil immersion objectives (Oil)
considered difficult so far until now for The objectives discriminated by the engrav-
such high magnifying powers. Furthermore, ing “Oil” are to be immersed in oil between
image flatness has been attained to an the specimen and front of the objective.
appreciable extent. When using oil immersion objectives of
(2) Plan Achromat (CF Plan) numerical aperture 1.0 or higher, it is rec-
Same as the above type, the objectives ac- ommended, for making full use of: its
complish the correction of chromatic efficiency, to use a highclass oil-immersion
aberrations based on the lines C and F. condenser such as of Achromat/aplanat
In addition, owing to sufficient correction type, applying oil between the glass slide
of all the image defects up to the periphery and condenser as well.

12
To see if air bubbles are present in the 2. Eyepieces
immersion oil, which deteriorate the image
To take full advantage of the CF eyepieces, use
quality, pull out the eyepiece from the
them in combination with the CF objectives.
eyepiece tube to examine the objective exit
The indication “CF” should serve to prevent
pupil inside the tube.
their use with other type objectives.
To remove air bubbles, revolve the nose-
piece slightly to and fro several times, 1) CFD eyepieces (CFD)
apply additional oil, or replace the oil. Being of wide field and high eyepoint type, the
Be careful not to rotate the nosepiece too CFD eyepieces are only used for observation,
far as to soil the ends of the other objec- obtains prominent image flatness. Compared
tives with oil. with the CFW eyepieces, they accomplish the
To clean off the oil, pass lens tissue or soft good correction of chromatic aberrations at the
cloth moistened with xylene lightly two or periphery of the viewfield in combination with
three times over the lens. It is essential at the low magnifying power of CF Plan
this time to avoid touching the lens with Apochromat objectives.
the part of tissue or cloth once used. They are equipped with a diopter ring and a
Any remnants of oil left on the lens dete- rubber eyeguard. An eyepiece CFD 10XM,
riorate the image quality. incorporating a photo mask, is also available,
(2) Coverglass which enables focusing and framing by the use
With the objectives engraved “160/0.17”, of the observation tube of the Trinocular
use a coverglass of 0.17mm in thickness Eyepiece Tube “T”.
(No. 1%). For the objectives whose N.A. is
2) CFW eyepieces (CFW)
0.75 or higher, a coverglass of other thick-
Being of wide field and high eyepoint type, the
ness than 0.17mm will deteriorate the CFW eyepieces with diopter ring are only used
image definition and contrast.
for observation. They are equipped with a
The indication 160/— on the objective
rubber eyeguard.
means that no matter whether a coverglass
An eyepiece called CFW 10XM, incorporating
is used or not, no decrease of image defini-
a photo mask is also available, which enables
tion or of contrast will result.
focusing and framing by the use of the observa-
(3) Objectives with compensation ring
tion tube of the Trinocular Eyepiece Tube “T”.
When a high power, dry objective of large
N.A. is adopted in combination with a 3) CFUW eyepiece (CFUW)
coverglass of thickness other than 0.17mm, Featuring extra-wide field of view and high
which will cause sharp reduction of image eyepoint, this eyepiece with diopter ring is
definition and contrast, it is necessary to designed exclusively for observation. It enables
use an objective incorporating a compensa- observation over a field of view twice as large as
tion ring as below: that of the ordinary type eyepieces in combina-
First, observe with the compensation ring tion with the ultra-wide tube.
set to 0.17, and then rotating the ring, An eyepiece called CFUW 10XM, incorporating
focus the image with the fine focus knob, a photo mask, is also available, which enables
until an image of the highest sharpness and focusing and framing by the use of the observa-
contrast is obtained. tion tube of the Ultra Wide Eyepiece Tube
(4) No-coverglass objectives (NCG) “UW".
Objectives with the indication NCG are 4) CF PL Projection lenses (CF PL)
suited for observing specimens such as Exclusively designed for photomicrography.
Do
smears without coverglass. not use them for observation.
(5) Objectives with aperture diaphragm Every eyepiece is liable to gather dirt and dust,
The objective incorporating an iris dia- which not only appear as shadows but also
Phragm serves to cut off direct light in impair image quality and contrast.
darkfield microscopy. Stop down the Keep the eyepieces clean at all times.
diaphragm nearly to its minimum opening.

13
3. Condensers with the CFW 10X eyepiece, it is possible to
close the field diaphragm down to 45% of the
1) Abbe condenser
viewfield.
N.A. = 1.25. This is used with 4X ~ 100X ob-
jectives. The graduation of this condenser 4) Darkfield condenser (Oil)
indicates the diameters in mm of the aperture N.A. = 1.43 ~ 1.20. Oil system. Used in dark-
diaphragm opening. field microscopy. Apply oil between the
condenser and glass slide. (It is recommended
2) Swing-out Achromat condenser
to use a thinner glass slide.)
N.A. = 0.9. Dry system.
This condenser is used in combination with the
It is used in combination with objectives from
objectives 10X ~ 100X with aperture dia-
2X to 100X, and provided with a swing-out top
phragm (N.A.: up to 1.1).
lens which is to be swung out when using the
2X or 4X objective. Its adjustable aperture scale 5) Darkfield condenser (Dry)
is graduated in N.A. ratings. N.A. = 0.95 ~ 0.8. Dry system. Used in dark-
3) Achromat/aplanat condenser field microscopy. Magnifying powers of usable
N.A. = 1.35. Oil system. objectives are 10X ~ 40X(N.A.: up to 0.7).
The spherical, coma and chromatic aberrations
being ideally corrected, this large aperture 4. Illumination System (Fig. 15)
condenser is used with 20X ~ 100 X objectives.
The optical system for illumination in the
The standard thickness of glass slide should be
LABOPHOT microscope is constructed to fulfill
1.2mm. the Koehler illumination requirements perfect-
Apply oil between the condenser and glass slide. ly, and offers a bright, uniform field without
It is recommended that this condenser be any change-over manipulation. i
employed especially in combination with the
As a standard light source, use the Halogen
Plan Apochromat objectives. When using the
lamp 6V 20W (PHILIPS 7388).
100X objective for observation in combination

Tube prism BY iece

OPTICAL PATH

Objective

Condenser
‘Aperture diaphragm

Filter
Collector-
lens i Field lens
abe

Halogen Diffuser Field Mirror

lamp diaphragm

Fig. 15

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У. РНОТОМИСВОСВАРНУ
(The Biological Microscope LABOPHOT is Failure” of film may result. So, when taking
designed mainly for observation.) picture of such specimens, it is recommended
to use the Nikon Biological microscope OPTI-

1. Combination of CF Objectives and PHOT.

CF PL Projection lens
The combined use of the CF objectives and CF 5. Manipulation of Field and Aperture
PL Projection lens is essential.
Diaphragms
For the same total magnification, select a In photomicrography, the adjustment of the
combination of the highest possible objective field diaphragm is important for the purpose of
power and lowest possible projection lens limiting extraneous light which causes flare in
power to achieve the utmost image definition the microscope image. Stop down the dia-
and contrast. phragm so as to get an illuminated area slightly
larger than that of the picture field. By adjust-
ing the aperture diaphragm, a change of depth
2. Checking the Illumination
of focus, contrast and resolution of image is
Uneveness in the illumination will show up attainable. Select a size suited to the purpose.
more conspicuously in photomicrography than Generally speaking, the aperture diaphragm, is
in observation. Consequently, before taking a properly stopped down to 70 ~ 80% of the
photograph, recheck the correct adjustment of aperture of the objective being used.
the condenser.

6. With Regard to Condensers

3. Selection of Voltage and Filter
For photomicrography, it is generally rec-
1) When using a daylight type color film ommended to use the Swing-out Achromat
Set the brightness control dial to 5.5 and use condenser. When using 2X objective, however,
the NCB10 filter* preferably remove the condenser.
Adjustment of the image brightness should be
made by means of the ND filters.
7. Focusing
2) When using a monochrome film
Focusing is to be accomplished by means of the
Remove the NCB 10 filter. Contrast filters such
ocular finder on the photomicrographic attach-
as X-1 green are usable.
ment, or binocular observation tube with mask
* The NCB 10 filter is most suitable for a eyepiece on the trinocular eyepiece tube.
standard film. Depending upon the make
of the film different color renditions may Table 3. Focusing
result. It is recommended that in addition Type of Focusing with Focusing with
to the NCB 10 filter a color compensation eyepiece | 10x or 4x or o
tube higher objective lower objective
filter (CC filter), available from the film
Use Ou 」 Focusing
manufacturer, be used. “Fº tube Ocular cular + va
finder finder magnifier
Use
4. Shutter Speed “qe observation
Use E tube Focusing
Desirable shutter speeds for least vibration are Or observation or .
“UW" tube! tube Ocular magnifier
1/4 ~ 1/15 sec. finder
Adjustment of the image brightness for color
photomicrography should be made by means of
the ND filters. Some specimens require, on
account of their insufficient brightness, longer
exposure times, and consequently poor color
reproducibility owing to the “Reciprocity Law

16
OD Adjust diopter. the double cross line so as their relative
position is fixed and unchaned under
e Binocular of eyepiece tube:
observation by swinging your eye laterally.
Use 4X or 10X objective.
(Focusing by parallax method.)
Insert the mask eyepiece into either of
right or left eyepiece sleeve that is ac- Using 4X or lower objective:
customed to usual use. Adjust the diopter Attach the focusing magnifier to the
ring to bring the double cross line in the ocular finder. (Fig. 18)
view field center into focus. (Fig. 16)
Then focus the specimen image also on the
central area of the mask by means of the sã pitt!
Focusing magnifier
focus knob of the microscope.
Ocular finder
The diopter of another eyepiece is to be
adjusted by focusing specimen rotating the
diopter ring without using the microscope
focus knob.
Rotate the mask eyepiece so as the mask Clamp screw
positions as shown in Fig. 19.
e Ocular finder:
Fig. 18
Adjust the diopter ring so as the double
cross line in the view field center can be Viewing through the attached focusing
seen clear and each line separated. (Fig. 17) magnifier, move it back and forth until the
double cross line is seen clear. Then, focus

Double cross line

Il LE
|| the .double cross line and the specimen
image by rotating the fine focus knob as
of the mask eyepiece | | | | sharp as possible.

Picture composing
Fig. 16
Compose the picture within the mask in
the ocular finder corresponding to the film
size in use by driving the microscope stage
Double cross line や多 > Y by lateral and longitudinal movement and
of the ocular finder 4 x “ o rotation. (Fig. 19)

Fig. 17 For 35mm film

For 4"x5"
(© Make focusing according to the magnifica- Polaroid film
tion of objective to be used.
For 3%" x4%"
© Using 40X or higher objective: Polaroid film

With diopter adjusted eyepiece make the For 6x9

roll film
specimen image sharp by rotating the ‘Double cross line
microscope fine focus knob and make sure Finder mask
that both of the double cross line and the Fig.19
specimen image are seen crisply at the same
When the mask eyepiece is used, select one
time.
out of masks in the view field suitable to
© Using medium magnification objective the film size relative to CF PL Projection
10X, 20X, etc.: lens in use, in reference with Fig. 20 and
After focusing the same way as above, Table 4.
bring the specimen image to coincide with

17
© For the use of other photomicrographic
attachment refer to the pertinent instruc-

L=
Inner frame
tion manuals.
Intermediate

Y Outer frame

Mask of the mask eyepiece

Fig. 20

Table 4
F E
CFPL ilm size

Mask |Projection| 35 |6x9 |3%"X|4" x | 6x6

lens mm | cm | 4%" | 5" | ст

2.5X 一一一一一

Inner 4X = = © — =
frame
5x o|-|-|0|-
2.5X 一 © © 一一
Inter-
mediate| 4X © 一一 © 一
frame
5x = ー - - =

2.5X © 一一 © 一
Outer 4X 二一一 = ー
frame
5x = = = = m

Note: Framing for picture composing will

be more accurate by the ocular
finder than the mask eyepiece.

9. Vibration-free operation
Set the microscope on a vibration-resistant,
rigid desk or a bench with a vibration-proof
device.

10. Others
© When using the 1X objective, place the
diffuser (available on order), and remove
the condenser.
e For photomicrography, when focusing
with the binocular observation tube, use
the CF eyepiece, CF PL Projection lens and
CF photo mask eyepiece, with the magnifi-
cation and other indications engraved in
yellow, or in white with a white dot in
addition.

18
VI. USE OF THE ACCESSORIES
1. Ultra Wide Field Trinocular Eyepiece 2. Polarizing Filter Set “PT”
Tube “UW”
1) Nomenclature (Fig. 22)
1) Objectives
CF Plan Achromat 2X ~ 100X, CF Plan Apo-
chromat 2X © 100X, CF Plan Achromat for
phase contrast 10X— 100%, CF Plan Achromat
for metallurgical 5X ~ 100X, CF Plan Apo-
chromat for metallurgical 50* or CF BD Plan
Achromat for bright and darkfield 5X ~ 100 Analyzer Polarizer

are used.
Fig. 22
2) Condenser
Refer to the Table 1 (P. 8). 2) Assembly
3) Assembly and microscopy (1) Attaching the analyzer

Assembly and microscopy being almost the After removing the eyepiece tube, insert
same as that of the regular microscopy (P. 6 the analyzer into the optical path hole in
and P. 8), only the differences will be described the microscope arm. (Fig. 23)
below. The white index dot is to be brought into
(1) Using the centering telescope coincidence with the Y-axis (of X-Y co-
For attaching the centering telescope on ordinates), viewing the arm from above.
top of the eyepiece sleeve, it is necessary to x
use the adapter (Fig. 21), because the tele- White 7,
index dot © Analyzer
scope which has been originally designed
for centering the annular diaphragm in
phase contrast microscopy, has a fitting
diameter different from that of the CFUW
eyepiece.

“Centering
telescope Fig. 23

(2) Condenser
Use the Swing-out condenser.
(3) Attaching the polarizer
As shown in Fig. 24, fit the polarizer to the
internal diameter at the bottom of the
Fig. 21 condenser.

Fig. 24

19
(4) Objective (NOTE)
Use the ordinary CF objectives. The following accessories can not be used in
combination with LABOPHOT (Y-R stand)
3) Microscopy Microscope.
(1) Turn ON the power switch. Set the bright-
@Teaching Head and Multi-teaching Head
ness control dial to 4.
(Only when they are combined with Ultra
(2) Remove the dust cap and place the daylight
Wide Eyepiece Tube “UW”)
filter.
eEpi-illuminator “M”
(3) Place the specimen on the stage and focus
on specimen with 10 Xobjective.
(4) Adjust the interpupillary distance and
diopter. (Refer to P. 9)
(5) Swing in the top lens of the swing-out
condenser in the optical path. (If using 4X
objective swing out the top lens.)
(6) Center the condenser. (Refer to P. 10)
(7) Rotate the polarizer until the darkest field
of view is obtained.
(8) Set the brightness control dial to 5~ 6.
(9) Change over the objective to be used and
sharpen the focus on the specimen.
(10) Adjust the aperture diaphragm and field
diaphragm. (Refer to P. 10 and 11)

20
Vil. TROUBLE SHOOTING TABLE
Although nowhere the user can find any disorder or derangement in the instrument, if he
encountes some difficulty or dissatisfaction, recheck the use, referring to the table below:

1. Optical

Failures Causes —>

一 Actions

Darkness at the Optical path in trinocular tube not——> Changing-over to the limit
periphery or fully changed-over < (Refer to P. 9)
uneven bright- Revolving nosepiece not in click-———— Revolve it to click-stop position
ness of view- stop position (Objective not
field centered in optical path)
(No appearance Condenser not centered Centering by using field dia-
of viewfield) phragm (Refer to P. 10)
Field diaphragm too much closed: Open it properly
Dirt or dust on the lens —————————— Cleaning
(Condenser, objective, eyepiece, slide)
Improper use of condenser ———_——> Correct use (Refer to P. 10)

Dirt or dust in Dirt or dust on the lens ——————————>> Cleaning

the viewfield (Condenser, objective, eyepiece, field lens)
Dirt or dust on the slide —————————5 Cleaning
Too low position of condenser ——————> Correct positioning
(Refer to P. 10)

No good image No coverglass attached to slide or Correct use (Refer to P. 13)

obtained (low NCG objective used with coverglass
resolution or Too thick or thin coverglass Use specified thickness (0.17mm)
contrast) coverglass (Refer to P. 13)
® Immersion oil soils the top of dry—————> Cleaning
system objective (especially 40X)
© Dirt or dust on the lens Cleaning
(Condenser, objective, eyepiece, slide)
© No immersion oil used on immersion——— Use immersion oil
system objective (Refer to P. 12)
® Air bubbles in immersion oil—— Remove bubbles
@ Not specified immersion oil used Use Nikon immersion oil
®@ Condenser aperture and field diaphragm—— Close properly (Refer to P.10, 11)
too much opened
@ Dirt or dust on the entrance lens —————> Cleaning
® Compensation ring in objective not—_> Adjustment (Refer to P. 13)
adjusted
®@ Objective aperture (which provided) ———> Open properly
too much closed

Image quality @ Condenser aperture too much closed——— Open properly (Refer to P. 10)
deteriorated Too low position of condenser———————, Bring it up to coincidence with
field diaphragm image
(Refer to P. 10)

Oneside dim- Revolving nosepiece not in click-————> Revolve it to click-stop position

ness of image stop position

21
Failures Causes ————____________. Actions

Image moves Specimen rises from stage surface —————> Place it stable
while being © Revolving nosepiece not in click ————> Revolve it to click-stop position
focused stop position
e Condenser not correctly centered————— Correct centering (Refer to P.10)
e Optical path in trinocular tube ——————= Changing-over to the limit
not fully changed-over (Refer to P. 9)

Image tinged e Daylight filter not used Use daylight filter

yellow

2. Manipulation

Failures Causes Actions

No focused e Upside down of slide Turn over the slide

image obtained | @ Too thick coverglass 一 Use specified thickness (0.17mm)
with high pow- coverglass (Refer to P. 13)
er objectives

High power ob- | © Upside down of slide ———————————= Turn over the slide
jective touches | e Too thick coverglass ——————————— Use specified thickness (0.17 mm).
the slide, when coverglass (Refer to P. 13)
changed-over e Eyepiece diopter not adjusted Diopter adjustment
from low power (Especially when changing-over (Refer to P. 9)
low power objective 1X or 2X)

Insufficient e Eyepiece diopter not adjusted Diopter adjustment

parfocality of (Refer to P. 9)
objective
(when changed-
over)

Movement of e Slide holder not tightly fixed ——————= Fix it tightly

image not
smooth by
moving the
slide

Travel of stage e |mproper attaching of slide Shift the attaching position

limited to one holder
half length of
slide

No fusion of e Interpupillary distance not Adjustment (Refer to P. 9)

binocular adjusted
images

Fatigue of e Incorrect diopter adjustment———————> Correct adjustment (Refer to P.9)

observing eyes e Inadequate brightness of illumination ——> Change power voltage

22
3. Electrical

Failures Causes ーーーーーーーーーー> Actions

Lamp does not | e No electricity obtained —————————= Connect the cord to socket
light even e No lamp bulb attached Attaching
though @ Lamp bulb blown一 Replacement
switched ON @ Fuse blown -一
一 Replacement

Unstable e Input voltage not adjutted to——————— Turn the change-over switch on
brightness of house current voltage (for European the microscope bottom
illumination districts only)
© House current voltage fluctuates—; Use transformer or the like
too much (for adequate voltage)

Lamp bulb e Not specified lamp bulb used — Use 6V 20W specified lamp bulb:
promptly (Halogen bulb: PHILIPS 7388)
blown e Too high voltage of house current
— Use transformer for adjustment

Insufficient @ Condenser not centered

一 Centering (Refer to P. 10)
brightness of e Condenser aperture too much closed— Open it properly (Refer to P. 10)
illumination @ Too low position of condenser ——————> Correct positioning
(Refer to P. 10)
e Not specified lamp bulb used Use 6V 20W specified Halogen
bulb (PHILIPS 7388)
e Dirt or dust on lens (condenser, —————— Cleaning
objective, eyepiece, field lens, filter)
e Too low voltage —————————————=> Raise the voltage

unstable e Lamp bulb not inserted to the limit———— Secure connection
brightness of @ Fuse holder not firmly fastened —————- Firm fastening
lamp bulb e Irregular change of house current Use stabilizer
voltage
| @ Lamp bulb insufficiently inserted —————> Positive connection
into the socket

4. Photomicrography

Failures Causes Actions

No sharp @ Improper focusing—— e Viewing into the finder and turning diopter ring,
picture bring double crosshair into focus. Moving the eye
obtained laterally, rotate fine focus knob, until no parallax
separation appears between the image and double
crosshair.
e At lower magnifications use focusing telescope in
addition.

23
Failures Causes——> Actions

No sharp e Out of focus — e For preventing external vibration, use vibration-

picture (Especially with high proof table or rigid desk.
obtained power objective and ㆍ Select a place free from vibrations, such as caused
long-exposure) by traffic, passers-by or motors etc.
© Momentary vibration—e Using ND filters or others, elongate exposure time
(for color film, to 1/4 ~ 1/15 sec.)
Lower the voltage, and elongate exposure time (for
black-and-white film).
Note, however, for color film, that lowering of
color temperature and change of spectral character-
istics will be unavoidable.
© Incorrect thickness—e Use a standard coverglass of 0.17mm in thickness.
of coverglass (No. 1%)
(Especially, when e Use objective with coverglass thickness compensa-
using large N.A. and tion ring.
high power objective)
e Using dry objectives — e Use no-coverglass type objective.
for smear preparations @ If other objectives are to be used, place a coverglass
on the specimen.

Fogging of © Grease, dust or dirt—— @ Clean the front of objective thoroughly, top surface |
image on optical surfaces of projection lens, specimen, photographic lens,
condenser lens, field lens, etc.

Illuminated e Condenser not————e Centering (Refer to P. 10)

image not centered
uniformly (This shows up
more conspicuously
in photography than
in observation)

image opened too large aperture stopped down to 70 ~ 80% of N.A. of the
contrast objective being used. (Refer to P. 10)
e Incorrect use of———© In metallurgical, interference, polarizing or phase
filter contrast microscopy, use of a green filter or mono-
chromatic interference filter (e.g. peak wavelength
= 546nm, half-value range = 30 nm) will increase
contrast.
When contrast is to be increased for a part stained
with a particular color, use a filter whose color is
complementary to the stain color (for black-and-
white film).
@ Inadequate use of @® Stop down field diaphragm to a diameter slightly
field diaphragm larger than the diagonal of picture frame.
(Refer to P. 16)

24
Failures Causes —————> Actions

image specimen contrast, darkfield, or differential interference
contrast methods.
@ Specimens should be stained a rather dark color, if
possible.
© In color photography, depending upon the speci-
men, red-blue separation staining (Mallory or Azan
methods etc.) is preferable to red-violet combina-
tion staining (H-E staining).
© In black-and-white photography, for low contrast
specimens a film of finer grain and higher contrast
is more suited (such as minicopy film).
© For general specimens a film of wider latitude and
finer grain is preferable.

resolving of objective © For the same magnification, increase power of
power of objective rather than that of eyepiece to attain
microscope higher resolution and sharpness, even though depth
of field is reduced.
© Excessive magnifi-—— © 500 ~ 1000 times N.A. are magnification limits for
cation resolving power.

Ghosts or © Extraneous light ——— © Darken the surroundings or place the cap on the
flare entering the ocular ocular finder.
appears finder
e Stray light entering—— e Take care not to expose microscope and specimen
to direct sunlight and other intense lights.

Poor photo- e Inadequate use of —— e Select best filter combination.

graph filter
obtained e Film of another e Note that, when using a daylight film, remarkably
make or emulsion NO, different spectral sensitivities will result depending
upon the type, make, etc.
Even though of same make, according to emulsion
number, different color rendition will be obtained.
e Wrong power ———— e Take picture in every case at the specified voltage.
source voltage (Refer to P. 16)
used
e Incorrect exposure—— e By inadequate exposure time, color rendition will
time not be true on account of ‘’reciprocity law failure”
Then, with the help of exposure time indicator,
adjust exposure time according to characteristics of
film by means of ND filters, or compensate for
such failure by means of CC filters.
(Refer to Kodak Data)
9 Influenced by ——— e Especially, for making color prints, it is recom-
film development mended to contact the development laboratory.

25
ELECTRIC SPECIFICATIONS
100V
Power source 120V 50/60 Hz
220/240V

Halogen lamp
6V 20W
(PHILIPS 7388)
100V
120V OV
1A (250V)
Fuse
220/240V 0.5A (250V)

We reserve the right to make such alterations in design

as we may consider necessary in the light of experience.
For this reason, particulars and illustrations in this
handbook may not conform in every detail to models in
current production.

27
NIPPON KOGAKU K.K.
Fuji Bldg., 2-3, 3 chome, Marunouchi,
Chiyoda-ku, Tokyo 100, Japan
203-214-5311
Telex: J22601 (NIKON)

Printed in Japan (85.6.B)H-E-3S

AA
CAUTIONS
[1] Avoid Strong Shocks!
Handle the microscope gently, taking
care to avoid strong shocks.
When Carrying the Microscope
When carrying the microscope, support
the bottom of the microscope base. The
instrument weighs about 7.9 kg. Do not
hold the overhanging portion of the base.
Place of Use
Avoid the use of the microscope in a dusty
place, or where it may be subject to
Vibrations,or exposed to high
temperatures, moisture, or direct
sunlight.
[4] Power Source Voltage and Fuse
Check the power source voltage and fuse
following the procedure on p.6.
[5] Light Source
The halogen bulb to be used is 6V-30W.
Do not use bulbs other than the one
specified in p.29 (electrical
specifications)
If a bulb of more than the suggested
wattage is used, the light adjusting circuit
may be damaged.
[6] Turning on the Lamp
Take care not to touch the lamp housing
when itis lit, and don’t bring inflammable
substances such as gasoline, thinner and
alcohol near it, as some parts of the lamp
housing may become very hot while the
Thank you very much for purchasing lamp is on
a Nikon Microscope. This microscope [7] Changing the Bulb and Fuse
is a high precision instrument with a Before replacing the bulb or fuse, turn
OFF the main switch and disconnect the
very delicate structure and varied
power source plug. Replace the halogen
functions. Please thoroughly read this lamp (6V-30W), making sure that it is
manual first to use the microscope cool enough. Do not touch the glass part
with your bare hands.
correctly.
[8] Dirt on the Lens
Do not leave dust, dirt, or finger marks on
the lens or bulb surfaces
They will prevent you from clearly
observing the specimen.
[9] Focus Knobs
Never attempt to adjust the tightness of
the right-and left-hand focus knobs by
turning one, while holding the other. It
may cause problems. Do not turn the
coarse focus knobs past the limit. Avoid
turning it forcibly, because it may cause
problems
CARE AND MAINTENANCE
[1] Cleaning the Lenses
To clean the lens surfaces, remove dust
using a soft brush or gauze. Only when
removing finger marks or grease, use a
soft cotton cloth, lens tissue, or gauze
lightly moistened with pure alcohol
(methyl alcohol or ethyl alcohol). For
cleaning the objectives of immersion oil
use only xylene. Do not use xylene for
cleaning the surface of the entrance lens
of the eyepiece tube or the prism surface CONTENTS
of the Ultra-Wide Eyepiece Tube "UW".
Observe sufficient caution in handling
I.NOMENCLATURE v5. 3
alcohol and xylene (they are in- II.ASSEMBLY +
flammable), and the ON-OFF of the
power source switch.
πΙ.ΜΙΟΒΟΦΟΟΡΥ -----
[2] Cleaning the Painted Surfaces
IV.MANIPULATION OF EACH
ELEMENT + НА 16
Avoid the use of any organic solvent (for
example, thinner, ether, alcohol) for 1) Use of focusing device ++ 16
cleaning the painted surfaces and plastic
parts of the instrument. We recommend 2) Optical path change-over in the
you use the silicon cloth. trinocular eyepiece tube + 17
Never Attempt to Dismantle! 3) Diopter adjustment
Never attempt to dismantle the 4) Interpupillary distance
instrument because you may impair the
functions. adjustment + ο... 18
When Not in Use 5) Centering the condenser lens---19
When not in use, cover the instrument 6) Use of field diaphragm
with the accessory vinyl cover, and store
it in a place free from moisture and 7) Use of condenser aperture
fungus. It is especially recommended diaphragm ----
that the objectives and eyepieces be kept
in an airtight container containing 8) Use of filters ++ 21
desiccant. 9) Oil immersion manipulation -----23
(5) Periodic Checking V .TROUBLESHOOTING +- 24
To maintain the best performance of the
instrument, we recommend that the ELECTRICAL SPECIFICATIONS.29
instrument be periodically checked. (For
details of this check, contact your au-
thorized Nikon distributor.)
Please note as per your Nikon warranty,
“Any defects or damage directly or indi-
rectly caused by the use of unauthorized
replacement parts and/or performed by
unauthorized personnel” will void the
warranty
I. NOMENCLATURE

Evepiece tube clamp screw

Mechanical stage

Specimen (glass slide) Hexagon wrench

Specimen holder
Coarse torque
Substage adjustment ring

Condenser centering screw

Fine-focus scale

Lamp housing

Filter receptacle

Line voltage indication plate

Lamp housing cover
Line voltage selector switch detaching button

Fig. 1-1
, I. NOMENCLATURE

Binoular eyepiece tube

Diopter ring

Eyepiece
Interpupillary
distance scale

Coarse focus knob Revolving nosepiece

Fine focus knob
Objective

Stage clamp screw

Condenser aperture
diaphragm ring

Condenser carrier

Condenser clamp screw

Brightness adjuster

Power switch

Condenser focus knob

Arm rest

Power cord

Fig. 1-2
II . ASSE M B LY (mounting and dismantling)

To assemble the microscope, follow the procedures given from to (2.

For details, read p.6 top.11.

(Optional)

Fig.2

5 Tools: hexagon wrench (accessory), plus screwdriver, minus screwdriver

II.ASSEMBLY

© Disconnect the power cord from the AC input connector. ( Make
Sure that it is disconnected.)
© Make sure that the line voltage indication plate at the rear of the base
indicates the correct line voltage to be used (100-120V or
220-240V) (Fig. 2-1).
When it indicates incorrect voltage, remove the line voltage
indication plate by unscrewing the two screws using a plus screw-
driver.
© Mount the line voltage indication plate after switching the input
voltage change-over switch to indicate correct voltage.

100V-120V or
220V-240V A

Fig. 2-1 Fig. 2-2

© Remove the fuse holder at the bottom of the base using a plus screw-
driver (Fig.2-2) and make sure that the metal part of the fuse
indicates “250V 1A" for 100-120V or"250V T1A" for 220-240V.
When incorrect fuse type is to be inserted, please contact
your authorized Nikon distributor.
@ Insert the fuse into the fuse holder and tightly screw it into the
original position using a plus screwdriver.

(2 Leveling Foot Screw

© For stable installation of the microscope, manipulate the adjustment
screw at the rear-right of the base (Fig. 2-3)
II.ASSEMBLY

Keep the cover on or use gloves when mounting the lamp bulb so
as not to touch the surface directly with your fingers. If finger marks
or dirt on the bulb surface, wipe them off with lens tissue. Make
sure to remove the cover after mounting the lamp.
@Remove the lamp housing cover pressing the cover detaching
buttons (Fig. 2-4)
@ Insert the lamp into the socket pin holes until it reaches the limit
(Fig. 2-5)
© Reattach the cover.

| 15. _흐
HA
Fig. 2-4 Fig. 2-5

Cautions when dismantling

e Turn off the power switch. (Make sure that it is off.) Do not touch
the bulb immediately after turning it off because itis very hot. Make
sure that the bulb has cooled enough when replacing it
II .ASSEMBLY

© Mount the objectives into the holes of the revolving nosepiece in
such positions that, when viewed from above, their magnifying
power increases as the nosepiece is revolved clockwise (Fig. 2-6)
Cautions when dismantling
@ Remove the ND filter cassette if attached and lower the stage by
turning the coarse focus knob
@ Remove any specimen if it is on the stage.
@ Hold the objective with both hands so as not to drop it when
removing it from the revolving nosepiece.

Optical path

Fig.2-6

@ Lower the substage by turning the coarse focus knob.
@ Loosen the stage clamp screw sufficiently.
@ Insert the stage into the circular dovetail of the stage mount of the
substage, [1] and [2]. Fasten the stage with the stage clamp screw
B (Fig. 2-7).
Specimen Holder
© The specimen holder should be fastened to the mounting hole on
the right side fora left-knob stage and on theleft side for a right-knob
stage. Remove the specimen holder when observing a large
specimen

@ Raise the substage by turning the coarse focus knob.
@ Lower the condenser carrier to its limit by turning the condenser
focus knob.
€ Insert the condenser lens into the condenser carrier [1] with the
numerical aperture plate facing toward the user. Fasten it with the
condenser clamp screw [2] (Fig. 2-8).
@ Raise the condenser carrier to its limit by turning the condenser focus
knob
II.ASSEMBLY

Cautions when Dismantlin

emove the ND filter cassette if attached
@ Lower the condenser carrier to its limit by turning the condenser
focus knob. Remove the condenser lens by releasing the condenser
clamp screw.

Fig. 2-9
@ Mounting the Binocular Eyepiece Tube
@ Loosen the eyepiece tube clamp screw sufficiently using the
hexagon wrench. (See @.)
O Insert the circular dovetail of the trinocular eyepiece tube into the
circular dovetail of the eyepiece tube mount. Fasten it with the
eyepiece tube clamp screw (Fig. 2-9)
Caution when clamping
© Do not hold the plastic part of the hexagonal wrench when
tightening the eyepiece tube clamp screw. Hold the stem of the
wrench. If overtightened, optical-path change-over of the
eyepiece tube may be malfunctioned

® Mounting the Eyepiece

@ Use the same magnification eyepieces for both the right and
left eyes
@ Insert the eyepieces into the sleeves of the binocular eyepiece tube
by engaging the three grooves of the eyepiece with the three
protrusions of the sleeve (Fig. 2-10)
@ When using eyeguard rubbers, insert them into the eyepieces (Fig
2-11)
Il. ASSEMBLY

Place the ¿45m filter on the filter receptacle of the field lens part

Fig. 2-12

@ Mounting and Dismantling the ND Filter Cassette

(optional)
@ Push down the ND filter cassette from the top so the two protrusions
of the lower part of the ND filter cassette fit with the two mounting
grooves on the rim of the field lens part of the microscope base
(Fig. 2-13).
@When frequently lowering the microscope stage, mount the ND
filter cassette in the direction as shown in Fig. 2-14.
@When dismantling, push the cassette either to the left or right and
raise the opposite side.

Fig. 2-14
II .ASSEMBLY

@ Insert the socket of the power cord to the AC input connector and
plug it into a AC line receptacle (Fig. 2-15).
@When the receptacle is not a double-pole ground type, use a plug
converting adapter (Fig. 2-16).

Adapter Socket

Ground spike

Fig. 2-15 Fig. 2-16

@ Hexagon Wrench
© The hexagon wrench is stored in the back of the microscope stand.
Take it out as shown in Fig. 2-17.
Il. MICROSCOPY
1) Put the NCB 1 filter (or necessary filter) on the filter
receptacle of the field lens part (Fig. 3-1).

2) Mount the ND filter cassette, if used (Fig. 3-2).

(Refer to II. ASSEMBLY-@ p.10)

3) Turn on the main switch to light the lamp.

Slide the brightness adjuster to meet with the 3rd line
from the right. (Fig. 3-3).

4) Slide the filter insertion“ removal knobs of the ND

filter cassette to the limit to place the entire ND filter
in the optical path (Fig. 3-4).

5) Revolve the revolving nosepiece to put the 10X

objective into the optical path. Properly turn the
revolving nosepiece so it fits into position (Fig. 3-5).

6) Place the specimen (glass slide) on the stage with its

cover glass facing up and fasten it with the specimen
holder (Fig. 3-6).
III. MICROSCOPY

7) Raise the condenser lens using the condenser focus knob

until it hits the limit (Fig. 3-7). When using an achromatic
aplanat condenser, add oil taking care not to allow
bubbles to exist between the top of the condenser lens and
the specimen
(See p. 23, Manipulation of Each Element-9).)

8) Fully open the field and aperture

diaphragms (Fig. 3-8).

9) Manipulate the Y-axis and X-axis stage motion control

knobs to put the specimen into the optical path (Fig. 3-9).
(Place the specimen above the condenser lens.)

10)When the trinocular eyepiece tube is used, change

the optical path of the eyepiece tube to enter 100%
of the light into the binocular part
(See p. 17, Manipulation of Each Element-2).)

11) Manipulate the coarse/fine focus knob and focus on

the specimen while looking in the eyepiece (Fig. 3-10).

2) Adjust the diopter (Fig. 3-11).

(See p. 18, Manipulation of Each Element-3).)

13) Adjust the interpupillary distance (Fig. 3-12)

(See p. 18, Manipulation of Each Element-4).)

Fig.3-12
Ill. MICROSCOPY

14) Focus and center the condenser lens (Fig. 3-13).

Image of field (See p.19, Manipulation of Each Element-5).)
diaphragm

15) Revolve the revolving nosepiece to the objective to be

used and focus on the specimen by manipulating the
fine focus or coarse focus knob. (See Note: 2.)
Image of field When using an oil immersion objective, add oil taking
diaphragm
care not to allow bubbles to exist between the top of the
objective and the specimen. (See p. 23, Manipulation
of Each Element-9).)
Eyepiece
2) viewfield stop
Fig.3-13
16) When the ND filter cassette is used, Adjust the brightness
by sliding the filter insertion / removal knob of the ND
filter cassette.
(See p. 21, Manipulation of Each Element-8).)
When the ND filter cassette is not used, adjust the
brightness of the lamp by manipulating the brightness
adjuster

17) Adjust the viewfield diaphragm and the aperture

diaphragm by manipulating their control rings.
(See p. 20, Manipulation of Each Element-6) and 7).)
Ill. MICROSCOPY

Note 1: Manipulate the centering screws of the condenser lens if part of the viewfield
is dark. If this doesn't help, check the following items:

* Insertion / removal of the ND Ш. MICROSCOPY-4)

filter cassette
* Turning the revolving mM. MICROSCOPY-5)
nosepiece(click-stop position)
+ Position of the condenser lens M. MICROSCOPY-7)
+ Viewfield and aperture diaphragms I MICROSCOPY-8)
fully open
+ Change-over of the optical path of II MICROSCOPY-10)
the eyepiece tube
+ Mounting the lamp I. ASSEMBLY-© Mounting the
lamp and the lamp housing
+ Mounting the condenser I. ASSEMBLY-© Mounting the
condenser
* Placing the filter on the filter I. ASSEMBLY-© Placing the filter
receptacle

Note2: Check the following items when the specimen cannot be focused.
+ Mounting the specimen Ш. MICROSCOPY-6)
+ Thickness of the cover
glass(standard=0.17mm)
W. MANIPULATION OF EACH ELEMENT
1) Use of Focusing Device
The turning direction of the knob and the direction of vertical
movement of the stage are shown in Fig. 4.
One rotation of the fine focus knob moves the stage 0. 1mm and the
graduation on the fine focus knob is 1 micron. One rotation of the
coarse focus knob moves the stage 12mm. The range of coarse
and fine motion is 2mm up and 28mm down from the standard
position
The coarse focus knob tightens by turning the torque adjustment ring
counterclockwise.
Never turn the right or left knob while holding the other. It may
cause problems.
Do not turn the coarse focus knob further than the limit.
IV. MANIPULATION OF EACH ELEMENT

2) Optical Path Change-Over in the Trinocular Eyepiece Tube

Note)Binocular eyepiece tube “B” does not have the optical path
change -over function. 100% of the light enters the observation
tube.
(1) When using the trinocular eyepiece tube “F”
As shown in Fig. 5-1, when the observation tube is turned toward
the user, 100% of the light enters the observation tube
As shown in Fig 5-2, when the observation tube is revolved 60 left,
100% of the light enters the vertical photo tube. In either case, turn
the tube to the limit.

Observation Vertical photo

tube: 100% tube: 100%

Fig. 5-1 Fig. 5-2

(2) When using the trinocular eyepiece tube “T”

As shown in Fig. 6, when the change-over knob is pushed until it
reaches the limit [1], 100% of the light enters the observation tube
When the change-over knob reaches the intermediate click [2], the
proportion of light entering the observation tube and photo tube will
be 14:86. When the change-over knob is pulled to the limit 3) ,
100% of the light enters the photo tube.

r1 Observation tube 100% |

17 BObservation tube:
photo tube= 14:86
/ м Photo tube 100%

Fig. 6
W. MANIPULATION OF EACH ELEMENT

3) Diopter Adjustment
(Before adjusting the diopter, implement MICROSCOPY procedures
1) to 11) and focus on the specimen with the 10X objective.)
Make diopter adjustments for both the right and left eyepieces
(1) Turn the diopter compensation rings on each eyepiece until the end
surface of the ring coincides with the engraved line
(This is the position of O dioptic compensation.) (Fig. 7-1)
(2) Swing the 40X objective by turning the revolving nosepiece and
bring the specimen image into focus by turning the fine focus knob
(or the coarse focus knob).
(3) Swing the 4X or 10X objective into position. Without manipulating
the fine and coarse focus knobs, turn the diopter rings on the
eyepieces so that the specimen images in the right and left
eyepieces are focused individually (Fig. 7-2)

End surface
Engraved line

Fig.7-1 Fig. 7-2

@ Repeat the above procedure two times, to adjust the diopter
perfectly.
© The above adjustment, compensating diopter difference between
the user's right and left eyes, will keep the tube length of the
microscope correct. This enables the user to take full advantage of
the high-quality objectives, including their parfocality.

4) Interpupillary Distance Adjustment

(Before adjusting the interpupillary distance, implement Microscopy
procedure 1) to 12) and focus on the specimen with the 10X
objective.)
Adjust the interpupillary distance, so that both the right and left
viewfields become one (Fig. 8)
This adjustment will enable the user to observe with both eyes.

Fig.8
IV. MANIPULATION OF EACH ELEMENT

5) Centering the Condenser Lens

(Before focusing and centering the condenser lens, implement
Microscopy procedure from 1) to 13) and focus on the specimen with
the 10X objective.)
(1) Close the field diaphragm to its smallest size by manipulating the
field diaphragm control ring. Rotate the condenser focus knob to
move the condenser vertically so that a sharp image of the field
diaphragm is formed on the specimen surface (Fig. 9-1,2 and 3).

Image of field diaphragm

Eyepiece viewfield stop

Fig.9-1 Fig.9-2 Fig. 9-3
(2) If the image decenters from the viewfield of the eyepiece, bring it
roughly to the center of the viewfield by means of the condenser
centering screws (Fig. 10-1 and 2).
(3) Change to the 40X objective. Focus on the specimen by turning
the fine focus knob and form an image of the field diaphragm on
the specimen surface by manipulating the condenser focus knob
(4) When the image decenters from the viewfield of the eyepiece, bring
it to the center of the viewfield by means of the condenser centering
screws. At this time, the centering will be facilitated by adjusting
the size of the image of the field diaphragm to be slightly smaller
than the viewfield of the eyepiece by manipulating the viewfield
diaphragm control ring (Fig. 10-3).

Image of field diaphragm

Eyepiece viewfield stop

Fig. 10-3
V. MANIPULATION OF EACH ELEMENT

6) Use of Field Diaphragm

The field diaphragm is used for determining the illuminated area on
the specimen. The size of the diaphragm is adjusted by manipulating
the field diaphragm control ring. Stopping down the diaphragm to
such an extent that the circumference of the illuminated area
circumscribes or inscribes that of the eyepiece field of view is
recommended for general microscopy. If a wider area than required
is illuminated, extraneous light will enter the field of view, causing flare
in the image and lowering the contrast. Therefore, especially in
photomicrography, the proper adjustment of the field diaphragm is
very important. Generally, good results will be achieved when the
diaphragm is stopped down to such an extent that the diameter of the
illuminated area is slightly larger than the diagonal of the film format.

7) Use of Condenser Aperture Diaphragm

The condenser aperture diaphragm is provided for adjusting the
numerical aperture (N.A.) of the illuminating system of the
microscope. Itis important because it determines the resolution of the
image, contrast, depth of focus, and brightness.
Stopping down the aperture diaphragm will lower the resolution and
brightness but increase the contrast and depth of focus. In general,
when it is stopped down to 70% to 80% of the numerical aperture
of the objective, a good image of appropriate contrast will be
obtained (Fig. 11)
To adjust the size of the condenser aperture diaphragm, manipulate
the diaphragm control ring referring to the condenser’s N.A. scale,
or after removing the eyepiece from the eyepiece tube, adjust the size
of the diaphragm, observing the image of the diaphragm that is
visible on the bright circle of the exit pupil on the inside of the objective.
Stopping down the aperture diaphragm too far will lower the
resolving power. Therefore, it is recommended not to stop down the
aperture to a size smaller than 60% of the N.A. of the objective in
use, except when observing almost transparent specimens

Exit pupil of objective

Aperture diaphragm

Size of the condenser aperture diaphragm

Fig. 11
WV. MANIPULATION OF EACH ELEMENT

8) Use of Filters
(1) ND filter cassette (Optional)
Accessory filters attached to the optional ND filter cassette are ND2,
ND4, and ND16. These are used for general microscopy and
brightness control in photomicrography.

| Np2 Reduce light to 1/2 Transmission rate=about 50%

| Np4 Reduce light to 1/4 Transmission rate=about 25%
ND16 Reduce light to 1/16 Transmission rate=about 6%

e Light is reduced to 1/8 when the ND2 and NDA filters are put into
the optical path and is reduced to 1/64 when ND4 and ND16 filters
are put into the optical path. Light can be reduced from 1/2 to 1/
128 depending on the combination of the filters.
e Securely change the filter insertion /removal knob.
Removing and mounting the ND filters
Use gloves or gauze so as not to touch the filters with your bare
hands.
To remove the ND filter, lay soft cloth such as gauze on a desk.
Place the ND filter cassette on it and spread the lever (Fig. 12).
To mount the filter, insert the filter obliquely in the opposite side of
the lever and spread the lever from the lower side
Any filters with an outer diameter of 45mm and thickness less than
3mm can be mounted in the ND filter cassette. The indication on
the filter insertion Z removal knob, however, can't be changed

Fig. 12
WV. MANIPULATION OF EACH ELEMENT

Transparent Cover
Mount the transparent cover to protect the filters from dust (Fig. 13-1).
When the ND filter cassette is removed, attach the transparent cover
on the field lens (Fig. 13-2). However, remove the cover during
observation or photomicrographing with the viewfield diaphragm
stopped down.

Fig. 13-1

(2) Filter receptacle

Filters with an outer diameter of 45mm can be placed on the filter
receptacle of the field diaphragm part. If the thickness of the filter
is less than 4.5mm, ND filter cassette can be mounted with the filter
placed on the filter receptacle.
Therefore, less frequently changed filters such as shown in the table
below are conveniently used placing them on the filter receptacle.

NCB 11 Color balancing filter

For general microscopy and color
photomicrography

For microscopy of phase

GIF Green interference filter difference, etc. For
monochrome-photomicrography
HE HE color contrast filter
For hematoxylin-eosin staining
For fuchsine staining
WV. MANIPULATION OF EACH ELEMENT

(3) Other filters

e 33mm Lemon skin filter
When the optional achromat condenser is used, insert the 633mm
lemon skin filter into the receptacle between the collector lens and
the lamp bulb. (Fig. 14-1)
@ ¢45mm heat absorbing filter
When the heat absorbing filter is used, mount it into the diffuser
holder inserted into the bottom of the microscope base

Lemon skin filter: | Heat absoring filter

AT O
^
E |
le
| 4

Diffuser holder o
L
Fig. 14-1 Fig. 14-2

9) Oil Immersion Manipulation

The objectives with the indication “oil” are to be immersed in oil
between the specimen and the front lens of the objective. Use only
the included oil. When using an oil immersion objective of numerical
aperture 1.0 or larger, it is recommended to use an oil immersion
type achromat/aplanat condenser to make full use of it's efficiency
For this type of condenser, apply oil between the specimen and the
condenser lens
Apply the minimum amount necessary (the quantity that fills the
gap between the front of the objective and the specimen, or
between the front of the condenser lens and the specimen) to avoid
a flow of excessive oil that will adhere to the stage and the
circumference of the condenser lens.
To see if air bubbles are present in the immersion oil, which
deteriorate the image quality, pull out the eyepiece from the
eyepiece tube. Fully open the viewfield diaphragm and the
aperture diaphragm to examine the objective exit pupil (bright
circle) inside the tube.
To remove air bubbles, slightly rotate the nosepiece several times
(or turn the condenser focus knob to move it slightly up and
down), or apply additional oil, or replace the oil.
Any remnant of oil left on the oil immersion objective or adhesion
of oil to the front of the dry system objective will deteriorate the
image quality. Clean off the oil after using it and make sure that
the oil did not adhere to the front of other objectives.
The oil for the condenser lens should also be cleaned off after use
To clean off the oil, wipe with lens tissue or a soft cloth, moistened
with xylene, lightly two or three times over the lens. It is essential
at this time to avoid touching the lens with a part of tissue or cloth
already used.
V . TROUBLESHOOTING
SEEING AND OPERATION
Failures Causes Actions
Optical path in trinocular
tube not fully
changed Changeover the optical path
securely to binocular
Optical path in trinocular tube not observation tube
changed-over for binocular (p. 17)
observation
Revolving nosepiece not in click-stop | Revolve it to click-stop position
Darknessatthe | Position (objective not centered in (put objective into optical
periphery. optical path) path) (p. 12)
U Position correctly so the
neven
brightness of Condenser is too low. viewfield diaphragm image is
viewfield. formed (p. 19)

Condenser not centered. Centering (p. 19)

No appearance
of viewfield. Condenser not mounted correctly. Mount correctly (p. 8)

ND filter not fully changed-over Changeover to the ne 21

Field diaphragm closed too much. | Open properly. (p. 20)
Improper combination of objective Use proper combination.
and condenser
Dirt or dust on the lens (field lens,
condenser, objective, eyepiece,
eyepiece tube entrance lens) or Cleaning. (p. 2)
specimen
Lamp not mounted correctly Mount properly (p. 7)
Vv. TROUBLESHOOTING
ーー ——

Failures Causes Actions

Position correctly so the
Position of condenser too low viewfield diaphragm image is
formed (p. 19)
Dirt or dust in | Aperture diaphragm too restricted. | Open properly (p. 20)
the viewfield. | Dir or dust on the lens (field
| lens, condenser, objective, 1 - 2)
eyepiece, eyepiece tube entrance Cleaning (p.
lens) or specimen.

| Aperture diaphragm too restricted. | Open properly. (p. 20)

Position correctly so the
Position of condenser too low. viewfield diaphragm image is
formed (p.19)
Too thick of thin coverglass Use specified thickness (0.17
No coverglass attached. mm) coverglass
NCG objective for observing Use normal objective for
specimen without coverglass used to | observing specimen with
observe specimen with coverglass | coverglass

Poor mage ea9

obtained P
observe specimen without
Use NCG objective.
(Contrast is too coverglass 1
strong or too |
| No immersion oil used on the front of |
weak.) | immersion system objective
| Use Nikon immersion oil
Details are not (p. 23)
| Immersion oil used not the type
clear.)
specified
Air bubbles in immersion oil Remove bubbles (p.23)

Immersion oil soils the top of dry Cleaning (p. 23)

system objective (especially 40X).
Compensation ring of objective not
Adjust to match coverglass.
adjusted
Dirt or dust on the lens (field lens,
condenser, objective, eyepiece,
Cleaning (p. 2)
eyepiece tube entrance lens) or
specimen.
V . TROUBLESHOOTING
—— —

Failures Causes Actions

Revolving nosepiece not in click-stop | Revolve it to click-stop

position position (p. 12)
One side of |
Place it stably in specimen
image is dim. _| Specimen rises from stage surface
holder on stage. (p. 12)
Stage tilted. Attach correctly (p.8)
Revolving nosepiece not in click-stop | Revolve it to click-stop
position position. (p. 12)
Place it stably in specimen
Image moves Specimen rises from stage surface.
while being holder on stage. (p. 12)
focused. | Condenser lens not correctly
|centered. Centering (p. 19)

Stage tilted Attach correctly (p. 8)

NCB11 filter not used. Use NCB11 filter (p. 12)
Image tinged
yellow. Lamp power source voltage too low. | adjust the lamp voltage by
manipulating the brightness
adjuster .Use the optional ND
Image too bright. | Lamp power source voltage too high. | filter cassette if the constant
| color temperature is to be
| desired
Lamp power source voltage too low. |
|
Aperture diaphragm too restricted. | Open properly. (p. 20)
Not bright | Position correctly so the
enough (see also | position of condenser too low. viewfield diaphragm image is |
Electrical). | formed. (p. 19)
Changeover so that 100% of
Optical path change-over
of trinocular light enters binocular part
tube not 100% binocular. (p.17)
Vv. TROUBLESHOOTING

Failures Causes Actions

Attach to stage with coverglass
up (when no coverglass,
No focused image Slide upside-down
specimen surface should be
obtained with high up). (p. 12)
power-objectives.
Use coverglass of specified
Coverglass too thick.
thickness (0.17 mm).

Attach to stage with coverglass

High-power up (when no coverglass,
objective Slide upside-down specimen surface should be
touches
up) (p. 12)
the specimen,
when changed-
Coverglass too thick.
Use coverglass of specified
over from low thickness (0.17 mm).
power.
Eyepiece diopter not adjusted Diopter adjustment. (p. 18)

Insufficient
parfocality of
Eyepiece diopter not adjusted. Diopter adjustment. (p. 18)
objective when
changed-over.

Movement of
image not smooth Slide holder not tightly fastened to Fasten it tightly.
when moving the stage,
specimen.

Travel of stage
limited to one-half Improper attachment of slide holder. Shift the attachment position.
length of slide.

Adjust interpupillary distance.

Interpupillary distance not adjusted.
(p. 18)
No cohesion of
Eyepiece diopter not adjusted. Diopter adjustment. (p. 18)
binocular image.
Magnifications of right and Use same eyepieces. (p. 9)
left eyepieces differ.

Interpupillary distance not adjusted.

Adjust interpupillary distance.
(p. 18)
Eyepiece diopter not adjusted Diopter adjustment. (p.18)

Experiencing eye Adjust the lamp voltage by

fatigue. manipulating the brightness
adjuster. Use the optional ND
Inadequate illumination.
filter cassette and adjust the
brightness with combination of
ND filters. (p. 21)
V. TROUBLESHOOTING

ELECTRICAL
Failures Causes Actions
No electricity obtained. Connect the cord to socket
No bulb attached. Attach lamp. 7
Lamp does not © Bulb attac + р tp. 7)
lighteven though | Bulb blown | Replacement. (p. 7)
switched ON. Τ
Fuse blown. Replacement (p. 6)
Lamp not correctly mounted. Attach securely (p. 7)

Bulb about to blow. Replacement. (p. 7)

e c t not secure Connect power source cord

Flickering and onnector securely (p.11)
unstable
“a on
illumination.
Fuse holder not firmly fastened. _| Fasten securely (p. 6)
Bulb insufficiently inserted into the
Socket Positive connection. (p. 7)

Bulb immediately
blown. Bulb used not the one specified. Use 6V 30W halogen 20)
Insufficient
illumination. |
Input voltage change-over switch Changeover input voltage
does not match the power voltage of
change-over switch. (p. 6)
| the room
Fuse blown.
Fuse used not the one specified. Use the fuse specified. (p. 6)
ELECTRICAL SPECIFICATIONS
Power source + 100-120V/220-240V, 50/60Hz
Halogen lamp + - 6V 30W [special type made by
PHILIPS ]
FUSE iii ++ 100-120V:250V 1A

220-240V:250V T1A
Power consumption - less than 43W

29
Nikon reserves the right to make such altera-
tions in design as may be considered necessary
in the light of experience. For this reason,
particulars and illustrations in this handbook may
not conform in every detail to models in current
production.
NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100, Japan
Tel: 81-3-3216-1026 Telex: J22601 (NIKON) Fax: 81-3-3201-5856

NIKON INC.
Instrument Group, Surveying Dept.
19601 Hamilton Avenue, Torrance, California 90502-1309, U.S.A.
Tel: (213) 516-7124 Telex: 967806 (NIKON GRCY) Fax: (213) 516-7751

NIKON EUROPE BY.

Schipholweg 321, P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands
Tel: 020-6594406 Telex: 13328 (NIKON NL) Fax: 020-6598335

Printed in Japan
M027 (91.5.E)H -E-4S

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Reparacion Microscopio 2

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Reparacion Microscopio 2

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Introduction to the Microscope

Operation and Preventive Maintenance

RUNNING TIME: 26:00 minutes

Table of Contents Videotape Counter

Eyepiece Tube Clamp Screw

Reverse Facing Nosepiece

Field Diaphragm Sharpens Image

interpupillary Distance Scale Eyepieces!

Condenser Control Ring

Condenser Centering Screws

Brightness Control Dial

2. Plug power cord into electrical socket.

3. Turn microscope on with brightness control dial.

5. Move specimen into light path with X/Y travel knobs.

6. Rotate to 10x objective

7. Adjust light intensity until it is at a comfortable setting for your eyes

9. Focus on image with coarse and then fine focus knobs.

10. Select a good viewing area using X/Y travel knobs.

11. Holdmilled ring on eyepiece stationary.

13. Refocus with fine adjustment knob.

14. Check focus on next highest magnification (40x).

15. Return to lowest magnification (4x).

16. Hold milled ring on eyepiece stationary.

Information on Objectives To Calculate the Final Magnification

Magnification of Magnification of Final

Rotate to 10x objective.

Raise condenser with condenser focus knob.

Switch to desired objective.

Recenter the field diaphragm image.

Rotate to 10x objective.

Select viewing area.

Rotate 10x out of light path.

Place drop of immersion oil above beam of light.

Adjust the fine focus.

If necessary, center and focus the field diaphragm image.

Enlarge field diaphragm image.

10. Adjust condenser diaphragm to fill 3/4 the tube.

11. Replace eyepiece.

12. Adjust light intensity. More light is required at higher magnifications.

13. Observe specimen.

Bulb not inserted properly Insert bulb correctly

Fuse not inserted properly Insert fuse correctly

Bulb not inserted properly Insert bulb correctly

Situation not covered above Call for repair

Too bright Light turned up too high Adjust brightness

Technical Consultants, Nikon Inc.

REPAIR PARTS LIST

HDA13310- “Product code No.

- 部 品 ーコ ド Parts code No.

皿 . 部 品 ( 組 品) の 注文 ーー… 3 How to order part(s)

Part (3), encircled by 2 dotted

line, but without each Index No. E

Spring Bin 3İ— 1:0 0! s |B.x スプ リン グ ビピン

Lub : Lubricant (# #4) E

1 2G301—007 Collector lens 1 5 @(GINEW)

4 2G702 一 009 Diffuser 1 5 ©

102 2K611—384 Arm rear cover 1 2 @

103 2K 406—085 Eyepiece tube receptacle 1 2 EE UM

12 2K362—017 Retainer 2 2 ©(#12, ®(#12, ©, ®

USA 2K600—191 Adjusting washer n 2 OC+15A), ©C215A), ©. ©

115C 2K600 一193 Adjusting washer n 2 O(#15C), @(#15C), ©. ®

112MB ¿388 (Common to 112MB)

115D 2K600—194 Adjusting washer O(+ 15D), O(+ 15D), ©, ©

119 2K021—046 HSB3 x 0.5 x6 9.6

125 2K625—410—1 Cover plate (Right) ©

140 C 120030 FC ©PM2x3

12MB¿ 368 (Common to 112MB)

142 C1—30060 FE @PM3 x 0.5 x6 3 3

201 2K115—524 Fine focus knob cap 2 3 es

202 2K201—128 Fine focus knob (Left) 1 3 @

206 2K631—323 Fine focus thrust stopper 1 3 ©, ©

(O: MBA 13500

230 28260-034 Gear 1 3 ©e

241 2K641—233 Cover ring 1 3

262 C1—30060 FE @PM3 x0.5 x6 6 3

301 2K680—242-1 Substage 1 4 Common to $201 in 112MB1

- 部品ーコド Parts code No.

皿 . 部品 ( 組品) の注文ーー… 3 How to order part(s)

Spring Bin 3İ— 1:0 0! s |B.x スプリングビピン

: MBC21100 と共通 (Common to MBC21100)

: MBC21103 と共通 (Common to MBC21103)

@ : MBC 21100 上共通 (Common to MBC21100)

@ : MBC 21100 と共通 (Common to MBC 21100)

@ : MBC 21100 と共通 (Common to MBC21100)