Nucleic Acid
Extraction Kits
"Plus" – larger volume of NA solution
"Rapid" – quick method
"Genetics" – human DNA extraction from whole blood
"DBS" – dried blood spots
Suitable for extraction of AVRI pathogens NA, including SARS-CoV-2
Quick NA extraction: from 15-30 minutes
Manual dosing
Automated dosing
Extraction time depends on biomaterial, number of samples, and
equipment being used.
Advertising and information material. For detailed information about the Nucleic Acid
Extraction Kit, please refer to the instructions for use
2
PREP-NA
Sample preparation Sample preparation
Line features
reagent kit product lines reagent kits
PREP-NA PREP-GS
PREP-NA PLUS
Lysis and further NA
PREP-NA PREP-NA-ULTRA
precipitation
PREP-NA-FET
PREP-NA-S PREP-
RAPID
PREP-GS
Higher purification Lysis and further NA
PREP-GS PREP-GS PLUS
degree sorption
PREP-GS Genetics
PREP-RAPID Thermocoagulation PREP-MB
Quick PREP-RAPID
PREP-RAPID Genetics of impurities
extraction
(express method) PREP-OPTIMA
PREP-OPTIMA Alkaline cell lysis
PREP-OPTIMA MAX
PREP-
Lysis with further sorption OPTIMA
PREP-MB on paramagnetic
nanoparticles
PREP-MB-NA-S PREP-MB DWP and
Automation ‣ Automated extraction PREP-CM
PREP-MB DWP PREP-MB-DBS DWP are
PREP-MB MAX compatible with KingFisher
‣ Manual or automatic Flex (Thermo Fisher Scientific),
PREP-MB RAPID
dispensing Auto-Pure 96 (Allsheng)
PREP-MB-DBS DWP PREP-
PREP-RADIP Genetics CITO DBS
Extraction from PREP-GS Genetics
Suitable for genetic assays
whole blood PREP-OPTIMA MAX
PREP-MB MAX Pre-
PREP-CM treatment
Extraction from PREP-MB RAPID Ability to extract from blood
cell cultures PREP-OPTIMA cultures – PREP-CM
PREP-OPTIMA MAX
Transport
Extraction from dried PREP-CITO DBS media
Suitable for genetic assays
blood spots PREP-MB-DBS DWP
Bio-
3 material
PREP-NA
Extraction of DNA and RNA (human, microbial and viral) by precipitation
Method: lysis and further NA precipitation
30* *
PREP-NA PREP-NA-ULTRA
Extraction time: from 30 min*, from 50 min Viral NA extraction from blood plasma with
Obtained NA sample volume: 50 μl preconcentration
* for nasopharyngeal and oropharyngeal swabs when using Extraction time: from 50 min
shortened extraction method for PCR detection of AVRI
pathogens, including SARS-CoV-2. Details of the method are
in the annex to the amplification part of the instruction for
SARS-CoV-2 kit. PREP-NA-FET
Fetal DNA extraction from mother's blood
30* *
PREP-NA PLUS Extraction time: from 2 hours
Extraction time: from 30 min*, from 50 min 25
Obtained NA sample volume: 300 μl PREP-NA-S
* for nasopharyngeal and oropharyngeal swabs when using
shortened extraction method for AVRI Complex. Details of Extraction of AVRI pathogens NA, including
the method are in the annex to the amplification part of the SARS-CoV-2
instruction for AVRI Complex kit. Extraction time: from 25 min
4
PREP-NA
PREP- PREP- PREP- PREP- PREP-
BIOMATERIAL
NA NA-PLUS NA-ULTRA NA-FET NA-S
Blood plasma
Urine
Feces
Nasopharyngeal/oropharyngeal
smears and swabs
Scrapes from
posterior pharynx
Phlegm
Saliva
Urogenital scrapes
Prostate fluid
Ejaculate
Сerebrospinal fluid
Milk
5
PREP-GS
Extraction of DNA (human and microbial) by sorption with extra purification
Method: lysis and further DNA sorption
PREP-GS
Extraction time: from 40 min
Obtained DNA solution volume: 100 μl
PREP-GS PLUS
Extraction time: from 40 min
Obtained DNA solution volume: 300 μl
PREP-GS Genetics
Extraction time: from 40 min
6
PREP- PREP- PREP-
BIOMATERIAL PREP-GS
GS GS PLUS GS Genetics
Whole blood
Blood plasma
Urine
Scrapes from posterior pharynx
Phlegm
Saliva
Urogenital scrapes
Prostate fluid
Ejaculate
Сerebrospinal fluid
Milk
Native tissues
7
PREP-RAPID
Express method of DNA extraction. Sample transportation and extraction in one tube
Method: thermocoagulation of impurities
15 20
PREP-RAPID PREP-RAPID Genetics
Microbial DNA extraction Human DNA extraction
Extraction time: from 15 min Extraction time: from 20 min
8
PREP- PREP-
BIOMATERIAL
RAPID RAPID Genetics
Whole blood
PREP-
RAPID
Urine
Scrapes from posterior pharynx
Saliva
Urogenital scrapes ♀*
Prostate fluid
Сerebrospinal fluid
* We do not recommend to use PREP-RAPID for DNA extraction from men's urogenital scrapes
9
PREP-MB
Extraction of DNA and RNA using paramagnetic nanoparticles
Method: lysis and release of NA under the action of guanidine thiocyanate with subsequent
sorption on paramagnetic nanoparticles and washing from impurities
PREP-MB RAPID PREP-MB MAX
DNA extraction DNA extraction
Extraction time: from 40 min Extraction time: from 60 min
Obtained DNA solution volume: 50-300 μl
PREP-MB-NA-S
DNA and RNA extraction
Extraction time: from 40 min
10
PREP- PREP- PREP-
BIOMATERIAL
MB RAPID MB MAX MB-NA-S
Whole blood
Urine
Feces
Nasopharyngeal/oropharyngeal smears and swabs
PREP-MB
Rectal scrapes
Urogenital scrapes
Ejaculate
Cerebrospinal fluid
Milk
Amniotic fluid
Ascitic fluid
Cell culture
11
PREP-MB
Extraction of DNA and RNA using paramagnetic nanoparticles
on KingFisher (Thermo Fisher Scientific) and Auto-Pure (Allsheng) instruments
Method: lysis and release of NA under the action of guanidine thiocyanate with subsequent
sorption on paramagnetic nanoparticles and washing from impurities
PREP-MB DWP PREP-MB-DBS DWP
Extraction of DNA and RNA DNA extraction from dried blood spots
of AVRI pathogens, including SARS-CoV-2 Extraction time: from 60 min
Total time of preparation for NA extraction and NA Compatible instruments: KingFisher Flex
extraction from 96 samples: from 40 min (Thermo Fisher Scientific), Auto-Pure 96 (Allsheng)
Extraction time: from 20 min
Obtained DNA solution volume: 50-300 μl
Compatible instruments: KingFisher Flex
(Thermo Fisher Scientific), Auto-Pure 96 (Allsheng)
12
PREP-
BIOMATERIAL PREP-MB-DBS DWP
MB DWP
Nasopharyngeal, oropharyngeal smears, swabs
Dried blood spots
Example of working with a large amount of samples using PREP-MB
PREP-MB DWP reagent kit
DeepWell preparation: RNA extraction:
20 min 20 min
DTstream KingFisher Flex (Thermo Fisher Scientific)
(DNA-Technology) Auto-Pure 96 (Allsheng)
13
PREP-OPTIMA
Extraction of DNA (human, microbial and viral).
Universal DNA extraction kit
Method: alkaline cell lysis occurring during thermal incubation
25 25
PREP-OPTIMA PREP-OPTIMA MAX
Extraction time: from 25 min Extraction time: from 25 min
Obtained DNA solution volume: 100-450 μl Obtained DNA solution volume: 100-450 μl
14
PREP- PREP-
BIOMATERIAL
OPTIMA OPTIMA MAX
Whole blood
Urine
Feces
Nasopharyngeal, oropharyngeal smears
Phlegm
Rectal scrapes
PREP-
Buccal epithelium OPTIMA
Urogenital scrapes
Ejaculate
Milk
Amniotic fluid
Synovial fluid
Native tissues
Fungal culture
Bacterial culture
Cell culture
15
PREP-CM
Bacterial and fungal DNA extraction from microbial cultures
Method: alkaline cell lysis occurring during thermal incubation
PREP-CM
Extraction time: from 40 min
Obtained DNA solution volume: 400 μl
BIOMATERIAL PREP-CM
Fungal culture
Bacterial culture
Cell culture
Blood culture
16
PREP-CITO DBS
Human DNA extraction from dried blood spots
Method: alkaline cell lysis occurring during thermal incubation. Removal
of possible impurities and stripping of blood from the carrier takes place
in the pre-washing stage
PREP-CITO DBS
Extraction time: from 40 min
DNA yield: 30-140 ng when extracted from 10 μl of blood dried on three filter paper discs
Amount of obtained DNA depends on the amount of leukocytes in sample
PREP-CM
BIOMATERIAL PREP-CITO DBS PREP-
CITO DBS
Dried blood spots
17
PREP-L
Lysozyme pretreatment of biomaterial before DNA extraction
Method: enzymatic destruction of peptidoglycans that make up the cell walls of
microorganisms, by lysozyme
Pretreatment time: Biomaterial for pretreatment:
from 30 min at t= 37 ⁰С ‣ Feces
from 60 min at t=18-25 ⁰С ‣ Meconium
‣ Bacterial culture from this biomaterial
Used together with PREP-MB MAX and PREP-NA PLUS NA extraction kits
PREP-FU
Biomaterial pretreatment to obtain lymphocytes from whole blood
Pretreatment time: 1 hour
Biomaterial for pretreatment: whole blood
18
PREP-PK
Biomaterial pretreatment by proteinase K before nucleic acid extraction
Method: proteolysis by proteinase K and elimination of inhibitory effects
Pretreatment time:
formalin-fixed, paraffin-embedded tissues: DNA — from 150 min,
RNA — from 60 min.
native tissues – 60 min;
cervical scrapes – 90 min.
Biomaterial for pretreatment:
‣ formalin-fixed, paraffin-embedded tissues (FFPE);
‣ native tissues;
‣ cervical scrapes taken into transport-fixating medium for liquid-based cytology
Used together with PREP-NA PLUS nucleic acid extraction kit
PREP-PK reagent kit is not intended for RNA extraction from biomaterial fixated in BD SurePath Pre-
treatment
transport medium.
19
STOR-F
Transport and storage of human biomaterial
Method: saline solution with the addition of a preservative that prevents the growth
of microorganisms
Suitable for further DNA and RNA extraction, including SARS-CoV-2 RNA
Compatible biomaterial:
scrapes/smears of epithelial cells from urogenital tract, oropharynx, nasopharynx, rectum, eye conjunctiva, skin
Transport and storage of biomaterial:
at t = 2 °С – 8 °С for no longer than 7 days
at t = 18 °С – 25 °С for no longer than 48 hours
20
STOR-M
Transport and storage of human biomaterial, including those containing mucus
Method: saline solution with mucolytic.
Preservative prevents non-specific microorganisms from reproduction, mucolytic affects
disulfide bonds of mucopolysaccharrides to thin mucus.
Suitable for further DNA extraction
Compatible biomaterial:
scrapes/smears of epithelial cells from urogenital tract, oropharynx, nasopharynx, rectum,
eye conjunctiva, skin, including those containing mucus
Transport and storage of biomaterial:
at t = 2 °С – 8 °С for no longer than 3 months
at t = 18 °С – 25 °С for no longer than 28 days
Transport
media
21
DNA RNA
NA extraction
BIOMATERIAL
reagent kits human microbial human microbial
PREP-GS Genetics
PREP-RAPID Genetics
Whole blood
PREP-MB MAX
PREP-OPTIMA MAX
PREP-NA
PREP-NA PLUS
PREP-NA-ULTRA
Blood plasma
PREP-NA-FET
PREP-GS
PREP-GS PLUS
PREP-NA
PREP-NA PLUS
PREP-RAPID
PREP-MB MAX
Urine
PREP-GS
PREP-GS PLUS
PREP-OPTIMA
PREP-OPTIMA MAX
PREP-NA
PREP-MB MAX
Feces
PREP-OPTIMA
PREP-OPTIMA MAX
PREP-NA
PREP-NA PLUS
PREP-MB MAX
Ejaculate PREP-GS
PREP-GS PLUS
PREP-OPTIMA
PREP-OPTIMA MAX
22
DNA RNA
NA extraction
BIOMATERIAL
reagent kits human microbial human microbial
PREP-NA
PREP-NA-S
PREP-MB RAPID
Nasopharyngeal, PREP-MB-NA-S
oropharyngeal PREP-MB MAX
smears PREP-GS
PREP-GS PLUS
PREP-OPTIMA
PREP-OPTIMA MAX
PREP-RAPID
Scrapes from
PREP-NA
posterior pharynx
PREP-NA PLUS
PREP-RAPID
PREP-NA
Saliva
PREP-GS
PREP-GS PLUS
PREP-MB RAPID
PREP-MB MAX
Rectal scrapes
PREP-OPTIMA
PREP-OPTIMA MAX
PREP-RAPID
PREP-NA
PREP-NA PLUS
PREP-MB MAX
Urogenital scrapes
PREP-GS
PREP-GS PLUS
PREP-OPTIMA
PREP-OPTIMA MAX
Bio-
23 material
“DNA-Technology”, LLC
[Link]
e-mail: info@[Link]
Client support service: +7 (495) 640-17-71
hotline@[Link]
