Dr.
DINESH KUMAR SHUKLA, CHIEF MANAGING TRUSTEE, PARA MEDICAL COUNCIL OF INDIA, OM
NAGAR, AMETHI ROAD,MUSAFIRKHANA, DISTRICT- SULTANPUR, U.P, INDIA 227813
PARAMEDICAL COUNCIL OF INDIA
POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY
DURATION: 2 YEARS + 3 MONTHS
LEVEL: DIPLOMA AFTER GRADUATION
TYPE: DIPLOMA
ELIGIBILITY: GRADUATION
POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY IS A POST GRADUATE
MEDICAL LABORATORY TECHNOLOGY COURSE. MEDICAL LABORATORY TECHNOLOGY ALSO
CALLED CLINICAL LABORATORY SCIENCE WHICH IS AN ALLIED HEALTH PROFESSION WHICH IS
CONCERNED WITH THE DIAGNOSIS, TREATMENT AND PREVENTION OF DISEASE THROUGH THE
USE OF CLINICAL LABORATORY TESTS. DURING THE COURSE SOME OF THE TOPICS ARE TO BE
COVERED. CLINICAL PATHOLOGY HEMATOLOGY. HUMUNOPATHOLOGY. MICROBIOLOGY, BLOOD
BANKING, HISTOPATHOLOGY AND CYTOLOGY. THE DURATION OF P.G.D. MEDICAL LABORATORY
TECHNOLOGY IS OF TWO YEARS BUT SOME INSTITUTES ARE OFFERING THIS COURSE ON THE
BASIS YEAR.
POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY
ELIGIBILITY
Candidate should have passed their [Link] with preferably chemistry/ zoology with
40% marks.
POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY SYLLABUS
Syllabus of medical laboratory technology as prescribed by various universities and
colleges.
Dr. DINESH KUMAR SHUKLA, CHIEF MANAGING TRUSTEE, PARA MEDICAL COUNCIL OF INDIA, OM
NAGAR, AMETHI ROAD,MUSAFIRKHANA, DISTRICT- SULTANPUR, U.P, INDIA 227813
FIRST YEAR
1ST PAPER
GENERAL LABORATORY AND INSTRUMENT MAINTENANCE
1. Organization of laboratory and safety precaution in laboratory and personal
cleanliness and care with regards to infected materials and clinical burns.
2. Quality assurance and disposal of wastes.
3. Cleaning of equipments and glass wares.
4. Maintenance and use of refrigerator, deepfrigers, incubators, ovens, water bath,
autoclaves, centrifuges, anaerobic chambers, BOD incubators etc.
5. Maintenance and knowledge of various components of microscopes and
application of various types of balances.
6. Principles and use of colorimeter, spectrophotometer, ELISA reader, turbidometers and
auto – analyzer etc.
7. Use and maintenance of balances.
8. Simple glass manipulation includes the making of capillary tubes, Pasteur pipettes, glass
sheet cutting.
2ND PAPER - CHEMICAL AND CLINICAL PATHOLOGY
1. Sample collection, preservation and transportation of various clinical pathology
samples.
2. Preparation of buffers, molar solutions, normal solutions and determination of ph of
buffers.
3. Biochemical estimation of total protein, albumin, globulin, sugar, cholesterol urea,
LFT, GTT, enzymes including preparation of their reagents.
4. Physical and chemical examination of urine including sugar, protein, acetone, bile
salts, bile pigments, blood, urobilinogen, chyle and microscopic examination for
crystals, cells and casts.
5. Examination of body fluids (CSF, ascetic fluid, pleural fluid, synovial fluid etc.)
Including biochemical, microscopic.
6. Semen analysis (count, motility, abnormal forms etc.)
Dr. DINESH KUMAR SHUKLA, CHIEF MANAGING TRUSTEE, PARA MEDICAL COUNCIL OF INDIA, OM
NAGAR, AMETHI ROAD,MUSAFIRKHANA, DISTRICT- SULTANPUR, U.P, INDIA 227813
3RD PAPER - HEMATOLOGY AND BLOOD BANKING
1. Collection of blood, preparation and use of different anticoagulant vials,
preparation of blood smears, staining of blood film bone marrow smear and
mounting of slides.
2. Preparation of reagents for hemoglobin, counting of leukocytes, RBC, platelet
and reticulocyte count, determination of ESR and PCV, and techniques of these
tests.
3. Recognition of blood cells in peripheral blood smears.
4. Preparation of haemolysate and determination of foetal hemoglobin and
hemoglobin electrophoresis.
5. Preparation of reagents and techniques for test of sickling, osmotic fragility,
G6PD enzyme deficiency, comb’s test.
6. Preparation of reagents and techniques of coagulation profile, platelet profile.
7. Blood donor selection and screening including ABO, RH blood grouping and
cross match tests.
8. Blood collection and preservation and component separation.
9. Identification of blood parasites (Malaria, Filarial, LD Bodies etc.)
Dr. DINESH KUMAR SHUKLA, CHIEF MANAGING TRUSTEE, PARA MEDICAL COUNCIL OF INDIA, OM
NAGAR, AMETHI ROAD,MUSAFIRKHANA, DISTRICT- SULTANPUR, U.P, INDIA 227813
SECOND YEAR
1ST PAPER - HISTOPATHOLOGY AND CYTOLOGY
1. Tissue reception. Labeling, fixation for different tissue and section cutting.
2. Preparation of paraffin blocks (dehydration, clearing, embedding, blocking.)
3. Handing and care microtome sharpening of razors, and section cutting.
4. Preparation of common stains H & E, congored, methyl violet, Leishman
stain, Giesma, Papiaicolau, VG , PAS, PASM etc. and staining techniques.
5. Mounting of museum specimens, record keeping, indexing of slides.
2ND YEAR - MICROBIOLOGY
1. Collection of samples, labeling and transportation of material and
disposal of microbiology products. Cleaning and methods of sterilization
of glassware’s media, instruments including syringes, needles and sharp
instruments.
2. Preparation of media, plugging of test tubes, preparation of swab sticks,
stains reagents, nutrient agar, blood agar, chocolate agar loeffer’s serum
dorsetts egg medium fellurile medium , L.J. Medium, peptone water
sugar media etc.
3. Processing of samples for isolation of bacteria from blood, CSF, tissue,
sputum, throat swab, sasal, eye swabs, aural swab, vaginal swab, wound
swab, rectal swab, urine, pleural fluid, ascetic fluid, AFB culture etc.
4. Biochemical reaction, acid production from fermentable substances
coagulase, catalase, oxidase, bile solubility optochin sensitivity, indole
production, M.R. test and V.P test. Urease activity citrare utilization etc.
5. Processing of sample for isolation of bacteria, blood , CSF, tissue sputurn
throat etc.
6. Drug sensitivity tests.
7. Staining method, gram stain, ziehl-nelsen stain, Albert’s stain etc.
8. Motility preparation of coci, corynebacteria, deptheria, mycobacteria,
gran negatice bacilli and gran positive bacilli.
9. Stool examination (occult blood), microscopic and culture.
10. Sputum examination (general, cytological and microbiological).
Dr. DINESH KUMAR SHUKLA, CHIEF MANAGING TRUSTEE, PARA MEDICAL COUNCIL OF INDIA, OM
NAGAR, AMETHI ROAD,MUSAFIRKHANA, DISTRICT- SULTANPUR, U.P, INDIA 227813
3RD PAPER - IMMUNOLOGY
1. Preparation of distilled water, deionized water and cleaning of distillation apparatus.
2. Collection of rabbits and sheep blood, animal care and raising of sera in animal.
3. Preparation of various buffers and reagents and techniques for following tests:
Agglutination tests
Haemagglutination tests
Precipitation tests and
Flocculation tests
4. Techniques of RA factor, CRP, ASO, VDRL, WIDAL, TORCH, auto antibodies, hepatitis, HIV
testing and EBV etc.
5. Complement titration, hemolysin titration, aldehyde test ELISA Test, serum electrophoresis,
gel electrophoresis, gel diffusion tests.
6. Preparation of slides of le cell phenomenon and identification.
7. Immune- histochemical staining methods for auto- antibodies and tumour markers.
8. Cutaneous sensitivity test.
