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Bioprocess Engineering Exam Questions

This document is an examination paper for the Fourth Semester B.Tech Degree in Bioprocess Engineering at APJ Abdul Kalam Technological University, dated June 2023. It consists of two parts: Part A with ten short answer questions and Part B with detailed questions from five modules, covering topics such as microorganism storage, fermentation processes, and bioreactor scaling. The exam is structured to assess knowledge on various aspects of bioprocess engineering with a total duration of 3 hours and a maximum score of 100 marks.

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Chinchu James
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119 views2 pages

Bioprocess Engineering Exam Questions

This document is an examination paper for the Fourth Semester B.Tech Degree in Bioprocess Engineering at APJ Abdul Kalam Technological University, dated June 2023. It consists of two parts: Part A with ten short answer questions and Part B with detailed questions from five modules, covering topics such as microorganism storage, fermentation processes, and bioreactor scaling. The exam is structured to assess knowledge on various aspects of bioprocess engineering with a total duration of 3 hours and a maximum score of 100 marks.

Uploaded by

Chinchu James
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

D 02000BTT206052102 Pages: 2

Reg No.:_______________ Name:__________________________


APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY
Fourth Semester [Link] Degree Supplementary Examination June 2023 (2019 Scheme)

Course Code: BTT206


Course Name: BIOPROCESS ENGINEERING
Max. Marks: 100 Duration: 3 Hours

PART A
(Answer all questions; each question carries 3 marks) Marks

1 Write the technical details for the storage of microorganisms under nitrogen. 3
2 What is the principle of in-vitro technique for the transfer of DNA between 3
different bacteria?
3 What are precursors? Why it is added in fermentation? 3
4 Summarize the characteristics of good industrial microorganisms. 3
5 Suggest the ways to avoid contamination in fermentation process. 3
6 What are the information’s required for the design of a batch sterilization 3
process?
7 Explain the characteristics of chemostat for a continuous culture. 3
8 Define volumetric mass transfer coefficient (KLa) in aeration process. Give its 3
physical meaning.
9 Mention the needs for modelling and controlling a bioprocess. 3
10 What is scale up of bio-reactors? List the environmental parameters involved in 3
scale-up studies.
PART B
(Answer one full question from each module, each question carries 14 marks)

Module -1
11 a) Explain the factors to consider in the selection of micro-organisms for a 10
fermentation process.
b) Write notes on lyophilisation method for storage of micro-organisms. 4
12 a) Describe the improvement of industrial micro-organisms by protoplast fusion 10
techniques.
b) What is site-directed mutagenesis? Why it is preferred than other techniques? 4

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02000BTT206052102

Module -2
13 a) With a schematic diagram, describe the important components of a typical 12
aerobic fermentation process.
b) Summarize the characteristics of wild – type microorganisms. 2
14 a) Explain the principle of solid state fermentation and submerged fermentation 10
process.
b) What are the objectives for optimizing fermentation media? 4
Module -3
15 a) Explain the kinetics of microorganisms death by steam sterilization method. 10
b) List the advantages of continuous sterilization over batch sterilization. 4
16 a) Describe the methods for the sterilization of fermenter exhaust air. 8
b) Explain the methods for the sterilization of liquid wastes. 6
Module -4
17 a) Explain the calculation of oxygen transfer rate from air bubble to the liquid 7
phase.
b) Discuss the procedure for the measurement of volumetric oxygen transfer 7
coefficient by oxygen-balance technique.
18 a) With neat sketch, explain the working principle of fed-batch operation of a mixed 8
reactor.
b) Explain the relationships between the operating variables and the concentrations 6
inside the reactor in a chemostat with immobilised cells.
Module -5
19 a) Explain the step by step approach to the modelling of biochemical process. 7
b) Brief the role of inoculation procedures and oxygen transfer rate on scale-down 7
of fermentation process.
20 a) Explain the scale-up of bioreactors based on constant power consumption per 10
volume and impeller tip speed.
b) Mention the process variables which affect mixing and mass transfer in 4
bioreactors.
***

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