Course Code: VI
DEVELOPMENT OF TOOTH
Dept. Of Dental Anatomy, & Dental
Histology
Govt. Dental College, Nagpur
Prepared by: Dr. Dipak Ghatage
Purpose Statement
• At the end of the lecture student should be able to
describe the Various stages of tooth development &
Morphology and function of various cells involved in
tooth development
Learning Objectives
• At the end of the lecture the student should be
able to
S.N. Learning Objectives Domain Level Criteria Condition
1 Enumerate and Cognitive Must Know All
described Various
histological
stages of tooth
development
2 Write about Cognitive Must Know All
Morphology and
function of various
cells involved in tooth
development
3 Draw sell labeled Cognitive & Must Know All
diagrams of Psychomotor
Development of teeth
CONTENTS
• HISTOLOGY OF TOOTH DEVELOPMENT
• STAGES OF TOOTH DEVELOPMENT
• 6 th week IUL: rupture of buccopharyngeal
membrane to form stomatodaeum
(primitive mouth)
• 37th day IUL: two thickened primary
epithelial bands, which subdivide to form:
dental lamina and vestibular lamina
• Localized proliferative activity within
dental lamina leads to formation of series
of epithelial ingrowths into
ectomesenchyme at sites corresponding to
position of future deciduous teeth.
HISTOLOGY OF TOOTH
DEVELOPMENT
STAGES OF TOOTH DEVELOPMENT
• Bud stage
• Cap stage (proliferation)
• Early bell stage (Histodifferentiation &
morphodifferentiation)
• Advanced bell stage
BUD STAGE
• Epithelial cells show little change in
shape or function.
• Supporting ectomesenchymal cells are
closely packed beneath and around the
epithelial bud.
CAP STAGE
• Condensation of ectomesenchyme immediately
adjacent to the epithelial ingrowth: due to local
grouping of cells that have failed to produce
extracellular substance & have not separated
from each other.
• It is already possible to identify the formative
elements of tooth.
• Dental/ enamel organ
• Dental papilla
• Dental follicle
EARLY BELL STAGE
• By 14th week further morpho and
histodifferentiation leads to early bell stage.
• Four types of epithelial cells that are
observed on light microscopic examination
of enamel organ are:-
1) Outer enamel epithelium
2) Stellate reticulum
3) Stratum intermedium
4) Inner enamel epithelium
OUTER ENAMEL EPITHELIUM
• Cells are cuboidal with large centrally
placed [Link] contain free
ribosomes,few profiles of ER , some
mitochondria & a few scattered
tonofilaments.
• Cells are separated from mesenchymal
tissue by basement membrane
• function
STELLATE RETICULUM
• In early development, cells
are sqamoid with blunt
processes. Their cytoplasm
contains usual complement of
organelles & tonofilaments.
• Cells continue to synthesize
and secrete
glycosaminoglycans and pull
water and there is increase in
volume of extracellular
compartment
STELLATE RETICULUM…
• As mucoid material accumulates cells in centre become
spindle or fusiform and those bordering the OEE and
stratum intermedium retain stellate shape.
• Cells contain ER,few mitochondria but well developed
golgi complex and they show presence of microvilli.
• Cells also secrete alkaline phosphatase & contain
small amount of RNA & glycogen.
• Functions-mechanical &determination of crown
morphogenesis.
STRATUM INTERMEDIUM
• 2-3 layers of squamous cells oriented at right angle to
developing ameloblasts.
• Oval or round cells with hyperchromatic nuclei and
few intercellular spaces housing microvilli.
• Few intercellular cell organelles.
• Cells are attached by desmosomes.
INNER ENAMEL EPITHELUIM
• Initially cells are 4-5 microns in width and 12 microns in
length
• Cells have large centrally placed nuclei with golgi apparatus
and centrioles at proximal end and mitochondria evenly
dispersed.
• Gap junctions between cells
• Cells continually divide until late bell stage. At the
sites of future cusp or incisal edge mitotic division
ceases and cell start differentiating.
DENTAL PAPILLA
• Acellular zone separates IEE and dental papilla.
• Cells of papilla are mesenchymal cells and fibroblasts.
• Until late bell stage dental papilla consists of closely packed
mesenchymal cells and show developing blood vessels.
• Rich in glycosaminoglycans.
• Cells of the DP start differentiating under the organizing
influence of IE.
DENTAL PAPILLA CONT…
• Differentiation begins at the site of deepest invagination
and progresses crestward.
• Preodontoblasts do not show well developed organelles
and specific orientation and are cuboidal.
DENTAL SAC
• Loose fibrous connective tissue investing enamel organ.
• Consists of fibroblast and mesenchymal cells.
• Intercellular spaces contain collagen fibrils.
• Dental sac is distinguished from dental papilla.
• Function.
SUMMARY
– Various stages of tooth development
– Morphology and function of various cells
involved in tooth development
Expected Questions
[Link] the morphological & histophysiological stages of development of tooth
and describe in detail about cap & bell stage. Draw well labeled diagram of cap
stage? (3+2+2+2=9 marks)
2. Enumerate the morphological & histophysiological stages of development of
tooth and describe in detail about early & advanced bell stage. Draw well labeled
diagram of bell stage? (3+2+2+2=9 marks)
Government Dental College and Hospital,
Nagpur
BIBLIOGRAPHY
Color Atlas And Text Book Of Oral Anatomy,
Histology Berkovitz, B. 1ST edition.
Oral Development and Histology Avery, j. K.1st
edition.
Orban's Oral Histology and Embryology Bhaskar, s.
N.11th edition.
Oral Histology : Development, Structure and Funct
Tencate, a. R. 4th edition.
Dental Embryology, Histology & Anatomy. Marry
Bath- Balogh Inergaret. 2nd edition.
Disclaimer
All data and content provided in this
presentation are taken from the reference
books, internet – websites and links, for
informational purposes only.
Government Dental College and Hospital,
Nagpur
