HKUGA College

Final Examination (2022/23)

BIOLOGY Total marks: 60

Question - Answer Book

Time allowed : 1 hour Form: 3

Name : _______________________________ Class (No.) : ( )

INSTRUCTIONS

 This paper consists of 14 pages including this cover page. The words “End of Paper” should appear on
the last page.

 Do not open this exam paper until instructed to do so.

 This paper consists of TWO sections, A and B. Section A consists of multiple-choice questions, which
carries 20 marks. Section B consists of conventional questions, which carries 40 marks.

 Answers to Section A should be marked on the Multiple-choice Answer Sheet while answers to Section
B should be written in the spaces provided in this Question-Answer Book. The Answer Sheet for Section
A and the Question-Answer Book for Section B must be handed in separately at the end of the
examination.

 The diagrams in this paper are not necessarily drawn to scale.

Section A: Multiple-choice question (20 marks)
Choose the BEST answer for each question and indicate the answer on the multiple-choice answer sheet
provided.
1. Kathy went diving in the sea. She saw a shark eating some small fish and another group of fish swam
away quickly. Which characteristics of living things are observed by Kathy?
A. nutrition, growth, movement
B. nutrition, sensitivity, movement
C. reproduction, sensitivity, excretion
D. sensitivity, movement, reproduction

2. On a field trip to a rocky shore, students observed that seaweeds grow on the upper shore (far from
the sea), while no seaweed grow on the lower shore (near the sea). Several students suggested
explanations for the lack of seaweed, such as:
 Seaweeds cannot grow near the sea because of the strong wave action.
 Invertebrates living on the lower shore eat all the seaweed.
The above suggested ideas are
A. observations.
B. hypotheses.
C. predictions.
D. conclusions.

3. John investigated whether the browning of cut apples is caused by exposure to oxygen. He prepared
six slices of apples. He immersed three slices in a vacuum container, while the other three slices were
left in a container with pure oxygen. Which of the following is the dependent variable of his
investigation?
A. degree of brownness
B. presence of enzyme
C. presence of oxygen
D. temperature of the experimental venue

4. Which of the following is / are the difference(s) between DNA and RNA?
(1) DNA has a phosphate group whereas RNA does not have.
(2) DNA has less oxygen molecules than RNA.
(3) Adenine is present in DNA whereas RNA does not have.
A. (1) only
B. (2) only
C. (1) and (2) only
D. (1), (2) and (3)

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 5. Which of the following correctly shows the suitable test for the organic substances?
Substance Treatment Result
A. Milk Dip the test end of Albustix The test end would turn green.
paper into the sample
B. Butter Grease spot test A translucent spot would form on the
filter paper, which would disappear
after drying.

C. Sucrose Benedict’s test Brick-red precipitate would form.

solution
D. Glucose Mix with iodine solution The mixture would turn blue-black.
solution

Directions: Questions 6 and 7 refer to the table below which shows the test results of three food samples.

Food sample
Test
X Y Z
Iodine test Blue-black solution Brown solution Blue-black solution
Benedict’s test Brick-red precipitate Brick-red precipitate Blue solution
formed formed
Grease spot test A translucent spot A persistent A persistent
formed and then translucent spot translucent spot
disappeared after formed formed
drying
6. Which sample(s) is/are likely to be potato chips?
A. X only
B. Z only
C. X and Z only
D. Y and Z only

7. Which of the samples is/are most likely an apple?

A. X
B. Y
C. Z
D. X and Z

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8. The photomicrograph below shows carbohydrate-containing structures (which are stained) in plant
cells.

Which of the following correctly identifies the type of carbohydrate stored in the structures stained
and its function?
Type of carbohydrate Function in plants
A. Glucose As a carrier of genetic information
B. Glycogen As an energy reserve
C. Cellulose As a component of plant cell wall
D. Starch As an energy reserve
9. A student wants to use a light microscope to observe a slide containing a paramecium, which is
shown below.

Which of the following correctly shows the image that would be observed by the student under a
light microscope?
A. B.

C. D.

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10. An animal cell placed in liquid X swells up and bursts. What will happen to a plant cell of same
water potential placed in the same liquid?
A. It will become turgid.
B. It will become flaccid.
D. It will not be affected.
C. It will become plasmolysed.

11. The following plant is wilting, and some leaves have turned yellow.

Which of the followings should be supplied to this plant to restore its health?
(1) Water
(2) Magnesium
(3) Carbohydrates
A. (1) only
B. (2) only
C. (1) and (2) only
D. (1), (2) and (3)

12. Which of the following statements correctly describe active transport and phagocytosis?
(1) They both require energy.
(2) They both require the help of carrier proteins.
(3) They can both transport materials regardless of concentration differences.
A. (1) and (2) only
B. (1) and (3) only
C. (2) and (3) only
D. (1), (2) and (3)

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13. The following electron micrograph shows a chloroplast.

(8,000X)
What is the actual length of this chloroplast?
A. 98.7 µm
B. 9.87 µm
C. 6.32 µm
D. 63.2 µm

14. The diagram below shows an apparatus in which the dialysis tubing is made of a differentially
permeable membrane.

What will happen to the liquid levels in the glass tubes after one hour?
Glass tube X Glass tube Y
A. falls rises
B. remains unchanged rises
C. rises falls
D. rises remains unchanged

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Directions: Questions 15 and 16 refer to the setup below.
The diagram below shows an experiment to investigate the digestion of lipids by lipase. This lipase can be
found in human small intestine. In this experiment, milk was used as the lipid source.

P Q R S

water bath at 25 ºC

The following table shows the contents of test tubes P to S.

Test tube Contents

3 3 3
P 4 cm milk + 1 cm lipase + 1 cm distilled water
Q 4 cm3 milk + 1 cm3 boiled lipase + 1 cm3 distilled water
R 4 cm3 milk + 1 cm3 lipase + 1 cm3 dilute sodium hydroxide solution
S 4 cm3 milk + 1 cm3 distilled water + 1 cm3 dilute sodium hydroxide solution

15. Which of the following combinations about the final pH of the contents in test tubes is incorrect?
Test tube Final pH of contents
A P slightly acidic
B. Q neutral
C. R slightly alkaline
D. S slightly alkaline

16. Which of the following would shorten the time required for the reaction to complete?
(1) Increase the temperature of the water bath to 37oC
(2) Reduce the amount of milk in each test tube by half.
(3) Add 1 cm3 of hydrochloric acid to each of the test tube.

A. (1) and (2) only

B. (1) and (3) only
C. (2) and (3) only
D. (1), (2) and (3)

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Directions: Questions 17 and 18 refers to the case below:
Three different enzymes (1, 2 and 3) catalyse a given reaction that produces product Z. The graph below
shows the amount of product Z produced by each enzyme over time. The data shown in the graph were
recorded during experiments where temperature, pH, and the amount of both substrates and enzymes were
controlled.

17. Which of the following is a valid description of the results shown in the graph?
A. Enzyme 1 sped up the reaction the most.
B. Enzymes 2 and 3 had lower kinetic energy than enzyme 1.
C. Enzyme 3 produced the least amount of product Z.
D. Enzyme 3 was denatured.

18. Which of the following points shows the highest reaction rate?
A. P
B. Q
C. R
D. S

19. Which of the following combinations about the industrial applications of enzymes is incorrect?
Application Enzyme used
A meat tenderizer protease
B. fruit juice extraction pectinase
C. production of lactose-free milk amylase
D. contact lens cleaner protease

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20. The following diagram shows how an inhibitor X works:

Which of the following can reduce the effect of this inhibitor on rate of enzymatic reaction?
(1) increasing the substrate concentration
(2) increasing the enzyme concentration
(3) decreasing the temperature at which the reaction takes place
A. (1) only
B. (2) only
C. (1) and (2) only
D. (1), (2) and (3)

End of Section A

Section B: Conventional Questions (40 marks)

Write your answers in the spaces provided.

1. For each of the biological processes listed in column 1, select from column 2 one membrane
transport mechanism for the process. Put the appropriate letter in the space provided. (3 marks)

Column 1 Column 2

Movement of oxygen from the (a) A. Diffusion

plasma into red blood cells B. Osmosis

Absorption of water into the (b) C. Active transport

capillaries of villi D. Phagocytosis

Ingestion of bacteria by white (c)

blood cells

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2. The diagram below shows the relative sizes of a virus and some cells.

(a) Which of the above cannot be observed by a light microscope? What kind of microscope should be
used to observe instead? (2 marks)

(b) State a structural difference between a bacterium and a eukaryotic cell. (1 mark)

(c) The diagram of animal cell is missing in the above diagram. Draw a labelled diagram of an animal
cell observable under a light microscope. (2 marks)

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3. The diagram below shows a cell extracted from the liver of an animal. This cell can produce an
enzyme called catalase.

B:________________

A
C

(a) Name organelle B in the diagram. (1 mark)

(b) Describe the role of organelles A and B in producing catalase. (2 marks)

(c) Explain how the rate of catalase production would be affected if the number of organelle C is greatly
reduced. (2 marks)

(d) The following diagram shows the action of catalase.

(i) Name molecule X. (1 mark)

(ii) What are molecules Y and Z? (2 marks)

(iii) Refer to the diagram, explain one characteristic of enzyme. (2 marks)

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4. James compared the water potential of four liquids commonly found in the kitchen using naked eggs.
Naked eggs refer to eggs with their outer shell removed but with the inner selectively permeable
membrane remained. The naked eggs were immersed into the liquids for five hours. The table below
shows the results.
Mass of an naked egg Solution
Grape juice Soy sauce Corn syrup Vinegar
Before immersion 95 g 92 g 94 g 93 g
After immersion 61 g 46 g 50 g 125 g
Percentage change in mass –35.8% –46.8%
(a) Calculate the percentage change of the naked eggs mass for Soy sauce and Vinegar and fill in the
above table. (2 marks)
(b) Arrange the water potential of the four liquids in ascending order. (1 mark)

(c) What should James do before weighting the naked eggs? How would the data be affected if this is
not done? (2 marks)

(d) Explain why there is a decrease of mass of naked egg when it is immersed in grape juice. (3 marks)

(e) Predict the result if the naked egg is put in distilled water. State the reason. (2 marks)

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 5. Mary is interested to know about how temperature affects the action of protease, an enzyme that
digests protein which can be found in stomach.
Eight solid protein strips are placed in test tubes with a neutral buffer solution and protease. Then
they were put in water-baths of 8 different temperatures. After 30 minutes, the protein trips were
taken out and weighted. The results are as follows:
Temperature (°C) Final weight of the protein trip (g)
0 8.1
10 6.8
20 5.4
30 4.2
40 3.5
50 6.1
60 8.1
70 8.1
(a) Draw a graph to show the results of the experiment. Include the appropriate axis labels and title.
(4 marks)

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(b) Based on the data, Mary suggested that the optimum temperature of the protease is 40 oC. Do you
agree? Explain briefly. (2 marks)

(c) Explain the experimental results at 0oC (3 marks)

(d) If the student has mistakenly used a strong alkaline buffer solution instead of neutral buffer solution
for this experiment, how would the results be affected? Explain briefly. (3 marks)

End of Paper

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