INTERNATIONAL

FOREWORD

IS0 (the International Organization for Standardization) is a worldwide federation

of national standards institutes (IS0 Member Bodies). The work of developing
International Standards is carried out through IS0 Technical Committees, Every
Member Body interested in a subject for which a Technical Committee has been set
up has the right to be represented on that Committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work.

Draft International Standards adopted by the Technical Committees are circulated

to the Member Bodies for approval before their acceptance as International
Standards by the IS0 Council.

as IS0 Recommendations;
iTeh STANDARD PREVIEW
Prior to 1972, the results of the work of the Technical Committees
these documents
were published
are now in the process of being
transformed
Committee
into International
ISO/TC 54 has reviewed
Standards. ([Link])
As part of this process, Technical
IS0 Recommendation R 1041 and found it
suitable for transformation. International Standard IS0 therefore replaces IS0
Recommendation R 104 I- 1969. ISO 1041:1973
[Link]
IS0 Recommendati on R 1041 was approved by8fe4e5d703b0/iso-1041-1973
the Member Bodies of the
folio wing countries :

Argentina Germany Portugal

Australia Greece Roman ia
Austria India Spain
Belgium Italy Sweden
Canada Morocco United Kingdom
Chile Nether lands U.S.S.R.
France New Zealand Yugoslavia

No Member Body expressed disapproval of the Recommendation.

0 International Organization for Standardization, 1973 l

Printed in Switzerland
INTERNATIONAL STANDARD IS0 1041-1973 (E)

Essential oils - Determination of freezing point

1 SCOPE AND FIELD OF APPLICATION 5.4 Apparatus assembly for determination of freezing
point, consisting of a wide-mouthed container of about
This International Standard specifies a method of 500 ml capacity, provided with a bored cork or rubber
determining the freezing points of essential oils. It is not stopper into which the stout-walled test tube (5.3) is
applicable in the special case of essential oil of rose. inserted. The test tube (5.2) is fitted into the stout-walled
test tube (5.3) by means of another bored cork or rubber
stopper. Into the test tube (5.2) a thermometer (5.1) is
2 REFERENCES
inserted, so that the centre of its mercury bulb is located at
IS0 2 12, Essential oils - Sampling. the centre of the liquid.

ISO/R 356, Essential oils - Methods of test - Preparation A suitable apparatus assembly is shown in the figure.
of sample.

3 DEFINITION iTeh STANDARD PREVIEW 6 SAMPLING

For the
following
purpose
definition
of this
applies :
([Link])
International Standard, the See IS0 212.

freezing point : Either the constant temperature, ISO 1041:1973

or the
maximum temperature, observed when the oil, in a
[Link]
7 PROCEDURE
supercooled liquid state, liberates its latent heat of fusion.
8fe4e5d703b0/iso-1041-1973
7.1 Preparation of test sample
4 PRINCIPLE See ISO/R 356.
Slow and progressive cooling of the essential oil.
7.2 Preliminary test
Observation of the variations in temperature as the oil
passes from the liquid to the solid state. If necessary, first liquefy the essential oil by warming. Cool
a few millilitres of the oil to be tested in a small test tube,
and stir with a thermometer until solidification takes place.
5 APPARATUS
Note the temperature and set aside in a cool place.
5.1 Calibrated thermometers, fulfilling the following
requirements : 7.3 Determination
length of bulb : 10 to 20 mm Fill the container (see 5.4) with water, melting ice or any
diameter of bulb : 5 to 6 mm suitable freezing mixture, so as to obtain a temperature
5 “C lower than that noted in the preliminary test. Fit the
graduations : 0,l “C stout-walled test tube (5.3) in its place.

It is essential that the set of thermometers used (see Into the test tube (5.2), place 10 ml of the essential oil,
ISO/R 653) should permit the measurement of any liquefied if necessary, insert the thermometer and carefully
temperature between - 20 and + 50 “C. cool the oil to the temperature indicated in the preliminary
test. Now insert the test tube (5.2) in the stout-walled
The relevant International Standards for the essential oils tube (5.3) of the apparatus and allow the temperature to
indicate which thermometer shall be used. fall a further 2 “C.
5.2 Test tube, about 20 mm in diameter and 100 mm Seed the oil with a trace of the solidified oil, obtained in
long. the preliminary test, and stir vigorously with the
thermometer, taking care to avoid the adhesion of particles
5.3 Stout-walled test tube, about 30 mm in diameter and to the walls of the tube. Observe the temperature variations
125 mm long. as carefully as possible.

1
IS0 1041-1973 (E)

Note the observed temperature when the temperature curve 9 TEST REPORT
in relation to time shows a maximum value, or a constant
ievel for at least 1 min. The test report shall include the following particulars :

Remove the test tube (5.2) from the apparatus and again
a) the reference of the method used;
liquefy the essential oil. Repeat the determination until two
consecutive results do not differ by more than 0,2 “C.
b) the results and the method of expression used;
Average the two last readings to obtain the final figure.

cl any unusual features noted during the

8 EXPRESSION OF RESULTS
determination;
The freezing point is the maximum temperature observed at
the end of the test. It is expressed in degrees Celsius to one d) any operation not included in this International
decimal place. Standard, or regarded as optional.

Dimensions in millimetres

iTeh STANDARD PREVIEW

([Link])
, Thermometer IS. 1)

ISO 1041:1973
[Link]
8fe4e5d703b0/iso-1041-1973 L----------
-- Test tube (52l

Stout-walled
test tube (5.3

Water, melting ice or

any freezing mixture

FIGURE - Suitable apparatus assembly for determination of freezing point