Starch Agar M107S
Starch Agar is used for detection of starch hydrolysing microorganisms.
Composition**
Ingredients Gms / Litre
Meat Extract 3.000
Peptic digest of animal tissue 5.000
Starch, soluble 2.000
Agar 15.000
Final pH ( at 25°C) 7.2±0.1
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at
15 lbs pressure (121°C) for 10 minutes. Mix well and pour in sterile Petri plates.
Principle And Interpretation
Starch Agar was formulated by Vedder (1) for the cultivation of Neisseria . It is recommended for the detection of starch
hydrolysing microorganisms from foods (2) and clinical samples (3). Present formulation is accepted by BIS for detection of
starch hydrolysis by Bacillus cereus (4).
Peptic digest of animal tissue and meat extract provide nitrogenous compounds, carbon, sulphur, trace elements etc. to the
microorganisms.
Flood the surface of 24 - 48 hour old culture on Starch Agar with Grams Iodine (S013). Starch hydrolysis is seen as a colourless
zone surrounding the colonies. A blue or purple zone indicates that starch is not hydrolyzed.
Quality Control
Appearance
Yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 2.5% w/v aqueous solution at 25°C. pH : 7.2±0.1
pH
7.10-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.(key *- On addition of Iodine solution)
Cultural Response
Organism Inoculum Growth Recovery Starch
(CFU) Hydrolysis *
Cultural Response
Bacillus cereus ATCC 10876 50-100 luxuriant >=70% Positive
reaction,
clearing around
the colony
Bacillus subtilis ATCC 6633 50-100 luxuriant >=70% Positive
reaction ,clearing
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
around the
colony
Escherichia coli ATCC 50-100 luxuriant >=70% Negative
25922 reaction , no
clearing
Staphylococcus aureus 50-100 luxuriant >=70% Negative
ATCC 25923 reaction , no
clearing
Streptococcus pyogenes 50-100 luxuriant >=70% Negative
ATCC 19615 reaction , no
clearing
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
1. Vedder, 1915, J. Infect. Dis., 16:385.
2. Harrigan W. and McCance M., 1976, Laboratory Methods in Food and Dairy Microbiology, Academic Press Inc. (London)
Ltd.
3. Lennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed., ASM,Washington, D.C.
4. Bureau of Indian Standards, IS : 5887 (Part IV) 1976.
Revision : 02/ 2015
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User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
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