1
Republic of the Philippines
Department of Education
Region VI – Western Visayas
Schools Division of Iloilo
Dueñas General Comprehensive High School
Poblacion B, Dueñas, Iloilo
POTENTIAL OF BOILED GUAVA (Psidium Guajava L.) LEAVES
EXTRACT AS SURFACE DISINFECTANT
Eguilos, Matt David P., Gerona, Chloe Anne L., Puerto, Jeanille Ann G.
INTRODUCTION
The increased concern over cleanliness has led to a greater demand for an effective
disinfectant. Chemical-based cleaning agents are widely used for surface disinfection, but they
often come with drawbacks such as toxicity, strong odors, and potential harm to the
environment and human health. These concerns have driven the search for natural alternatives
that are both safe and effective in cleaning unsanitary surfaces (Salonen et al., 2024).
Guava leaves (Psidium guajava) is a tropical plant widely recognized for its medicinal
properties. Its leaves, in particular, are rich in bioactive compounds such as flavonoids, tannins,
and saponins, which are known to exhibit antimicrobial and antibacterial activity. In traditional
medicine, guava leaves have been used to treat wounds, diarrhea, and various infections,
making them a promising candidate for natural disinfectants (Rojas et al., 2006).
Boiling guava leaves may enhance the release of these active compounds, potentially
increasing the antimicrobial potency of the extract. Since the process is simple, cost-effective,
and does not require sophisticated equipment, it presents an accessible option for households
and communities with limited access to commercial disinfectants. Utilizing boiled guava leaf
extract could therefore offer a practical, low-risk, and eco-friendly way to maintain surface
hygiene, especially in rural or low-income areas.
This study aims to explore the potential of boiled guava leaves extract as a surface
disinfectant by evaluating its effectiveness in terms of appearance, scent, and overall
effectiveness as natural disinfectant. By doing so, the research seeks to contribute to the
development of safer, sustainable alternatives to synthetic disinfectants while promoting the
practical use of natural resources in everyday hygiene.
Statement of the Problem
This research aims to investigate the potential of boiled guava (Psidium guajava L.)
leaves extract as surface disinfectant.
Specifically, it aims to answer the following research questions:
1. What is the effect of boiled guava (Psidium guajava L.) leaves extract as surface
disinfectant at different concentrations in terms of surface texture?
a. 10g of guava leaves and 100mL of water
2
Republic of the Philippines
Department of Education
Region VI – Western Visayas
Schools Division of Iloilo
Dueñas General Comprehensive High School
Poblacion B, Dueñas, Iloilo
b. 15g of guava leaves and 100mL of water
c. 20g of guava leaves and 100mL of water
2. What is the effect of boiled guava (Psidium guajava L.) leaves extract as surface
disinfectant at different concentrations in terms of odor?
a. 10g of guava leaves and 100mL of water
b. 15g of guava leaves and 100mL of water
c. 20g of guava leaves and 100mL of water
3. Is there a significant difference on the potential of boiled guava (Psidium guajava L.)
leaves extract as surface disinfectant at different concentrations in terms of surface
texture and odor?
a. 10g of guava leaves and 100mL of water
b. 15g of guava leaves and 100mL of water
c. 20g of guava leaves and 100mL of water
Hypothesis
There is no significant difference on the potential of boiled guava (Psidium guajava L.)
leaves extract as surface disinfectant at different concentrations; a. 10g of guava leaves and
100mL of water, b. 15g of guava leaves and 100mL of water, c. 20g of guava leaves and
100mL of water in terms of surface texture and odor.
METHODOLOGY
Research Design
This study entitled “Potential of Boiled Guava (Psidium Guajava L.) Leaves Extract as
Surface Disinfectant” is an experimental research study that will be conducted using a
descriptive survey, descriptive survey is an approach of descriptive research that blends
quantitative and qualitative data to provide you with the relevant and accurate information. The
experiment will be pursued to find an alternative solution to chemically modified disinfectants.
Procedure
A. Preparation of Materials
The materials for making boiled guava leaves extract as surface disinfectant will be
prepared. The following materials include: guava leaves, water, strainer, pot, containers,
measuring cup, stirring stick or spoon.
1. The guava leaves is the main material that will be used to make the surface
disinfectant.
2. The gram scale will be used to measure the weight of guava leaves in grams.
3. The water will be used to extract the beneficial compounds from the guava leaves.
3
Republic of the Philippines
Department of Education
Region VI – Western Visayas
Schools Division of Iloilo
Dueñas General Comprehensive High School
Poblacion B, Dueñas, Iloilo
4. The pot will be used to boil the guava leaves.
5. The measuring cup will be used to measure the right amount of water.
6. The stirring stick or spoon will be used to mix the ingredient thoroughly.
7. The strainer will be used to separate the boiled guava leaves from its residue.
8. The container will be used to store and preserve the boiled guava leaves.
B. Preparation of Ingredients
All the ingredients will be measured based on the different proportion of guava
(Psidium guajava L.) leaves and water volume. Guava leaves will be measured approximately
45g and 300mL of water.
C. Making of Boiled Guava (Psidium Guajava L.) Leaves Extract as Surface Disinfectant
1.1. The guava leaves will be gathered at Brgy. Baac, Dueñas, Iloilo.
1.2. The guava leaves will be measured through a gram scale.
1.3. The measured guava leaves will be washed thoroughly with water to remove
dirt.
1.4. The washed guava leaves will be placed in a pot with water.
1.5. After placing it in a pot, bring the water to a boil.
1.6. Then, reduce the heat and simmer it for 15 mins or until the liquid reduces and
turns a brownish color.
1.7. Lastly, wait for the temperature of the extract to decrease.
D. The Finished Product of Boiled Guava (Psidium Guajava L.) Leaves Extract as Surface
Disinfectant
The boiled guava leaves extract will be prepared. Using a measuring cup, the boiled
guava leaves extract will be poured into three containers with different concentrations.
E. Testing of the Boiled Guava (Psidium Guajava L.) Leaves Extract as Surface
Disinfectant
1. The researchers will travel to Dueñas General Comprehensive High School.
2. Upon the arrival, the researchers will prepare the necessary materials for the
experiment.
3. The researchers will look for 15 respondents to answer a survey.
4. Next, the researchers will prepare the different concentrations of surface disinfectant
and survey that will be given to the 15 respondents.
5. In each trial, different concentrations of surface disinfectant and a survey will be
given to the 15 respondents.
6. In each trial the respondents will record their own observation on the given survey.
4
Republic of the Philippines
Department of Education
Region VI – Western Visayas
Schools Division of Iloilo
Dueñas General Comprehensive High School
Poblacion B, Dueñas, Iloilo
7. After the respondents answered the given survey, the researchers will gather all the
results on the survey.
8. Lastly, the researchers will calculate all the result.
Data Gathering Procedure
Data will be gathered to access the potential of boiled guava (Psidium Guajava L..)
leaves extract as surface disinfectant consisting of two independent variables: odor and surface
texture. Odor is the process of evaluating the smell of a disinfectant to determine its
acceptability and any unpleasant smells. Surface texture process of assessing the changes in the
smoothness, roughness and overall texture of a surface after the application of a disinfectant.
The data of the study will be gathered accordingly and accurately using a survey that will be
given to 15 respondents.
Data Analysis Procedure
Data will be analyzed using ANOVA as the statistical tool. The study is experimental
research that will be conducted a series of observations and testing to measure the effectiveness
of the study. The experiment will be pursued to find an alternative solution to chemical-based
for surface disinfection.
