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Grossing Procedures in Histopathology

Grossing examination is a crucial process in pathology for inspecting specimens to gather diagnostic information before further analysis. It involves visual observation, description, and cutting of tissues, along with recording dimensions and digital images. Key considerations include specimen orientation, inking for malignant lesions, and detailed documentation of tumor characteristics prior to processing.

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181 views7 pages

Grossing Procedures in Histopathology

Grossing examination is a crucial process in pathology for inspecting specimens to gather diagnostic information before further analysis. It involves visual observation, description, and cutting of tissues, along with recording dimensions and digital images. Key considerations include specimen orientation, inking for malignant lesions, and detailed documentation of tumor characteristics prior to processing.

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GROSSING & TISSUE FIXATION

SURESH KUMAR MAHASETH


LECTURER (MLT)
DSEU,DWARKA CAMPUS
[Link]@[Link]
Grossing

 Grossing examination (Grossing) is a process , by means of which pathology specimens are inspected with
bare eyes to obtain useful diagnostic information , before being processed for further procedures &
microscopic examination .
 Grossing examination of surgical specimens is typically performed by a pathologist , or by a senior
laboratory technician .
 The purpose of grossing is also to confirm identity of the specimen & examine it for gross pathological
changes .
 Gross examination of the specimen is performed in a specific gross station (cabinet ) .
 The initial step in any examination of a clinical specimen is confirmation of the identity of the patient & the
anatomical site from which the specimen was obtained.
.
The Basic step involved in gross examination of the tissue are as follow

 Macroscopic examination of specimen – visual observation & description of the tissue.


 Cutting of the tissue representing specific lesion .
 Recording of digital image of the resected specimens.
Following various points are consider ed for gross examination of tissues

 Macroscopic examination of tissues: It involves visual observation , description & cutting of tissue representing lesion.
 Various aspects considered are , type of surgical procedure (e.g. Whipple’s resection , Wertheim's hysterectomy etc.), anatomic
site , specimen type (obtained by needle biopsy , punch biopsy etc.) & whether , the specimen received in the type of fixative
used.
 Dimensions: Length , breadth , width & weight of the specimen are recorded prior to dissection .
.
 Specimen orientation : Orientation of small specimens can be provided by placing the
base of the biopsy on a plastic small card paper (e.g. endoscopic biopsy) .

 Digital images of the resected specimens can be captured & recorded before inking & dissecting the specimen &
also of cut surface to demonstrate the lesion.
 Intaking Specimen: In Suspected malignant lesions the entire specimen is inked . All margins in resected
specimens are inked .In small specimens inking is helpful during embedding .
 Dissection : Small biopsies , endoscopic & needle biopsies are entirely submitted for subsequent processing . In
case of malignant tumors following points are noted before processing – The site & size of the tumor , location &
identity of structures invaded by the tumor , vascular invasion , distance from resection margins & the presence
of lymph nodes in the specimen .

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