Dextrose Broth M044
Intended Use:
Recommended for cultivation of wide variety of microorganisms.
Composition**
Ingredients Gms / Litre
Tryptose 10.000
HM peptone B# 3.000
Dextrose (Glucose) 5.000
Sodium chloride 5.000
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 23 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense
into tubes or flasks or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Dextrose in culture media serves as a source of energy. Dextrose Broth is useful when the organism has to be revived from
small inocula. Dextrose Broth can also be used for anaerobic growth by the addition of 0.1- 0.2% Agar. Agar, thus added,
helps to disperse the growth formed and also expel the CO2 formed (3). Facultatively aerobic organisms tend to grow near
the surface, in upper zone of the tube. Dextrose Broth is used for antibiotic sensitivity testing using the tube dilution method
(4). Sensitivity testing of neomycin and chlortetracycline is better done using this medium.
HM peptone B and tryptose serve as sources of nitrogenous compounds, sulphur, carbon, vitamins and minerals. Dextrose is
an energy source. Sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Food samples
Specimen Collection and Handling:
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per
established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual
safety data sheets.
Limitations :
[Link] medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured, clear solution in tubes
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Reaction
Reaction of 2.3% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
Organism Inoculum Growth Gas Growth (with
(CFU) 0.1% Agar)
Escherichia coli ATCC 50-100 good-luxuriant positive good-luxuriant
25922 (00013*) reaction
Neisseria gonorrhoeae 50-100 good-luxuriant negative good-luxuriant
ATCC 19424 reaction
Neisseria meningitidis ATCC 50-100 good-luxuriant negative good-luxuriant
13090 reaction
Staphylococcus aureus 50-100 good-luxuriant negative good-luxuriant
subsp. aureus ATCC reaction
25923 (00034*)
Streptococcus pyogenes 50-100 good-luxuriant negative good-luxuriant
ATCC 19615 reaction
Streptococcus pneumoniae 50-100 good-luxuriant negative good-luxuriant
ATCC 6305 reaction
Key : *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date
on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent
lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump
formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the
container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must
be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
Reference
1. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
2. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
3. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1,
Williams and Wilkins, Baltimore.
4. Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore Walsbren and Dunnette A., 1951, Am. J. Clin. Path., 21:884.
5. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods,
5th Ed., American Public Health Association, Washington, D.C.
Revision : 03/ 2019
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. [Link] : 23, Vadhani [Link]., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
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