STERILITY INDICATORS

PRESENTED BY: ANGGIETH KATHERINE MENDOZA

SURGICAL INSTRUMENTATION PROGRAM
UNIVERSITY OF SANTANDER
CLINICAL STERILIZATION CENTER SAN LUIS
COORDINADORA : ANGELA LATORRE
ENFERMERA JEFE : JANETTE OYOLA
 What is sterilization?

It consists of the destruction of all forms of microbial life, including spores (the spores
they are the microorganisms most resistant to heat, chemical agents, and radiation).
A sterile object is considered to be one that has undergone the different processes of
sterilization.
Before introducing the material into any sterilization system, it must be dry, clean and
well packed to prevent the passage of germs.

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 STERILITY INDICATORS

Sterilization indicators are devices or reagents aimed at certifying or

validate that the process was carried out properly. The indicators are classified as: physical,
chemical and biological.
OBJECTIVES:
Meet quality standards.
2. Verify that the equipment is sterile.
3. Verify that they have undergone a sterilization process.
4. Monitor the processes.

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 PHYSICAL INDICATORS

Time Time of
Pressure Control of sterilization vacuum dried
sterilization temperature

Source: author of the work

Pressure
caldera

They are measurement elements built into the sterilizer that allow visualization of whether the equipment has reached the
required parameters. E.g.: thermometer, stopwatch, load sensor, etc.
 Biological Indicators

spore Geobacillus
It is the most effective method It is a preparation characterized stearothermophilus:vapor
of a specific microorganism
what is there to verify the
resistant to a particular process Bacillus subtilis variety of
sterilization of a load
of sterilization niger: dry heat

These are distributed in the

{"cargas":"loads"}

It is expected to take 12 hours. Why does a biological come out? Vapor: where is it located?
and if the indicator continues to positive? Answer: because there it is
the autoclave drainage.
yellow color is sent to I cultivate a spore and therefore Peroxide and oxide: it is placed
study at the laboratory, if not, so much must be collected the in the middle of the load.
it is called a false positive load.
 BIOLOGICAL INDICATORS

Source: author of the work

Autoclave a Peroxide of oxide of

vapor hydrogen ethylene
 BIOLOGICAL INDICATOR SYSTEM FOR EXTRA FAST READING

Process indicator:
Located at the top
from the IB cover, facilitates the Audible alarm: the alarm
IB recognition of it sounds (and the icon blinks
control from the alarm) as soon
how to detect a
Access a the
positive result
information/Settings
customized: Press a
time, the date
and the serial number

Verification of
temperature: press a Minutes remaining in the cycle
time to check the temperature of incubation
current blocking of the
heater
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Positive results: it will appear the Negative results: a will appear /928117O/attest-biological-indicator-
plus symbol (+) as soon as it is detected minus symbol (–) once it is de-reading
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a positive result complete the incubation Biological of Le
READING OF THE BIOLOGICAL INDICATOR IN STEAM AT THE
INCUBATOR

Source: author of the work

 Chemical Indicators

They are devices that contain chemical substances that change color when exposed to a
or more critical variables of a sterilization process.
They ensure the correct operation of the sterilizer and that the internal conditions of the chamber
reach the sterilization parameters, allowing for early detection of failures.
Sterilization parameters:
•Vapor saturado: tiempo, temperatura, vapor saturado.
•Oxido de etileno: temperatura, tiempo, humedad relativa, oxido de etileno.
Dry heat: temperature and time.
Hydrogen peroxide: time, temperature, H202
 CLASSIFICATION OF CHEMICAL INDICATORS

Type 1 Process indicators:

Designed to show the
exposure to the process of
sterilization.

Source: author of the work

Type 2 Test indicators
specific.
(Bowie and Dick for
sterilizers with pump of
empty)

Source: author of the work

Type 3 Parameter indicators

simple: designed for
respond to a critical variable
of the process.
Type 4 Multiparameter indicators:
designed to respond to
two or more critical variables
of the process.

Source: author of the work

Type 5 Integral indicators:

designed to respond to
all the critical variables of the
sterilization process.

Source: author of the work

Type 6 Emulator indicators:

designed to respond to
all the variables of a
sterilization process of
specific way.
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 Bowie-Dick Test
Chemical Indicator Type 2

chemical indicator in diagonal lines, the

which changes from light yellow to
black once the sheet is processed. The sheet
it is placed in the center of a package of
Bowie-Dick test and processed at 134°C
for 4 minutes, with the autoclave without
load. It has a space for registration
of the department, operator, sterilizer
and results, and they are perforated for
facilitate its control in a log of
registration. The test is designed to
determine the penetration of the steam and the
functionality of the vacuum pump of
vacuum sterilizers. It has a
two-year expiration starting from the date
of manufacturing.

Source: author of the work

POSITIVE TEST: (accepted) the sheet
the entire sample shows a uniform change of
color (from yellow to black), which
means a rapid penetration of
vapor, a proper disposal of the
air and absence of significant leaks
of air. The intensity of the color is not
as significant as uniformity
of the change.

NEGATIVE TEST: (rejected)

presents when the color change is not
it is uniform due to air pockets
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trapped in the chamber, which indicates xeIgCEw/s1600/BowieDick_graphic.jpg

an incomplete removal of air from

the chamber or failures of the sterilizer for
maintain the vacuum during the cycle of
test.
Bowie-Dick Test
(Video)

Source: author of the work

 CHALLENGE PACKAGE TO THE PROCESS

Challenge Package to
process:

It is the test that is done

when is it going to be set up a With the reading IB
load in the autoclave. super fast is
they obtain results
Contains an indicator Source: author of the negative reliable
biological and an integrator work
after 1 hour of
class 5. incubation.

It is placed in the chamber,

about the drainage, near
the autoclave door.
Never should be put in the
middle of the camera.

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LOAD ASSEMBLY WITH A PACKAGE CHALLENGE TO
PROCESS
(Video)

Source: author of the work

 INDICATORS MANAGED IN SAN LUIS CLINIC

Chemical indicator
class 1

Chemical indicator Chemical indicator

class 4 class 5

Chemical indicator biological indicator

class 2

Source: author of the work

 TURN OF THE INDICATORS

The indicator tape helps us know if the material passed.

or not through a sterilization process showing us this at
change color as seen in the image.

Passing through a steam process, the tape is going to turn me from

green or beige to brown or black.

By going through an ethylene oxide process, the tape works for me.
to change from green to dark orange.

By undergoing a hydrogen peroxide process, the tape

It's going to change from blue to pink.

Source: author of the work

 The indicator strips are used to secure individually or
for package that the different parameters of each process
The sterilization has been completed inside each
one, showing us this when changing color like so
observe in the image.

By going through a steam process, the strip is going to turn me into

beige to black.

By going through a steam process, the strip will exceed the

limit which is the blue line with the black liquid that
it has inside.

Passing through an ethylene oxide process, the strip will

change from brown to olive green.

By undergoing a hydrogen peroxide process, the strip

it's going to change from blue to pink.

Source: author of the work

 BIBLIOGRAPHY

• [Link]
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• [Link]
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• [Link]
• [Link]
[Link]?fn=Attest Biological Indicator of Le