UNLOCK THE INNOVATION:
AGENDA
1. Emerging Trend In Aesthetics: why a paradigm shift is needed
2. Introducing PRX-PLUS: Multilayer Skin Booster
3. The treatment STEP by STEP
4. Standalone & Combined Procedures
Not just Volumizing!
Regenerating: A New Era in Aesthetics
Over the last decade, aesthetic medicine has shifted focus:
/ Not only volume replacement, but also skin stimulation.
/ Focusing on skin quality by restoring skin vitality.
This evolution reflects a growing demand for natural-looking, long-term results—not just
structural changes, but true skin regeneration.
Key Factors Defining Good Skin Quality:
It’s all about the Surface
Skin Firmness Skin Surface Evenness
Pore sizes
Elasticity Crepiness
Tightness Wrinkles &
Hydration Lines
Acne Scars
Pigmentation Radiance
Erythema Luminosity
Coloration/ Brighteness
Discoloration Complexion
Skin Tone Evenness Skin Radiance
by JE-YOUNG PARK, MD; JENG -FENG CHEN, MD; HOSUNG CHOI, MD; WILSON W. S. HO, MBChB, FRCS; NI NYOMAN INDRA LESTHARI,
MD;JOYCE TENG EE LIM, MBBS, FRCPI, FAMS (DERMATOLOGY); TING SONG LIM, MD; STEPHEN LOWE, MBChB, MPH; BEVERLY ONG -
AMORANTO, MD;VASANOP VACHIRAMON, MD; RUNGSIMA WANITPHAKDEEDECHA, MD; MARTINA KERSCHER, MD, PhD
Why Surface Matters: Importance of Superficial Layers
The superficial layers of the skin play a crucial role in maintaining overall skin quality.
Skin physiology teaches us that any
disruption of the skin barrier—whether
caused by thermal, mechanical, chemical, or
other external stressors—does not stop at the
surface.
Instead, it can trigger the synthesis of
signaling molecules that penetrate into the
dermis and activate regenerative
mechanisms.
Understanding and leveraging this natural cascade is fundamental for developing
treatments that promote skin regeneration and restoration without damaging
downtime.
AGENDA
1. Emerging Trend In Aesthetics: why a paradigm shift is needed
2. Introducing PRX-PLUS: Multilayer Skin Booster
3. The treatment STEP by STEP
4. Standalone & Combined Procedures
Why Surface Matters
• Pioneers of non-invasive medical aesthetic procedures
• From the Creators of PRX-T33.
• Created by dermatologist Dr Castellana for doctors.
• The Global Original Needle Free Skin Booster
• Made in Italy
• Globally Patented
• Trusted in Over 70 Countries
• Over 8 Million Procedures Performed Worldwide
• <0,0006% side effects
PRX-PLUS
THE FORMULA
33% AMMONIUM TRICHLOROACETATE
ATCA stimulates production of collagen type III, therefore it is responsible for the tightening effect.
2% HOMOCYSTEIC ACID
HoA is CELL REGENERATING and a SMART LIGHTENING ingredient.
CITRIC & PHYTIC ACID
PhyAc: ANTIOXIDANT – CHELATING – TYROSINASE INHIBITOR
CA: INCREASE INGREDIENTS ABSORPTION
How does PRX-PLUS work: A Multilayer Approach
First Layer: Stratum Corneum – The Smoothing Gateway
Citric acid facilitates the absorption
Epidermis:
HCA and phytic acid stimulate queratinocytes and melanocytes.
Dermis: Deep Stimulation Without Needles
ATCA activates fibroblasts for collagen types I and III.
Result: Visible rejuvenation, firm and luminous skin.
Collagen Regeneration Timeline
DAY
1–2 Activation of fibroblasts
3–7 Pro Collagen Synthesis
7–14 Collagen Deposition
15–30 Dermal Remodelling
30–60+ Cumulative effect up to 6 months
Action of ATCA on the Dermis
Once the citric acid softens the keratin at the stratum corneum level, all the other active ingredients, such as ATCA are able to reach
the epidermis.
ATCA First messenger: ATCA
It interacts with keratinocytes in the epidermis causing
Proliferation them to produce Growth Factors.
PDGF-B
Epidermis: TGF-β1 Second messenger: Growth Factors
Keratinocytes
TGF-α They migrate into the papillary dermis where they bind
VEGF to fibroblast receptors and stimulate their activation
and proliferation.
RESULT OF THE BIOCHEMICAL CASCADE:
o Producing new collagen (types I and III)
o Synthesizing hyaluronic acid and other glycosaminoglycans (GAGs)
o Reconstructing and remodeling the extracellular matrix (ECM)
Dermis
This regenerative process is essential to restore dermal architecture, improve skin elasticity, and enhance overall skin quality.
TIGHTENING EFFECT
H E R O I N G R E D I E N T:
HOMOCYSTEIC ACID
/ STRONG ACID / KERATINOCYTES / MELANOCYTES INTERACTION
INTERACTION
pKa HoA < pKa TCA
NMDA RECEPTOR NMDA RECEPTOR
SMOOTHING EFFECT AGONIST AGONIST
(KERATINOCYTES) (MELANOCYTES)
BRIGHTENING EFFECT
PRX PLUS
Clinical Results
Efficacy Tests on 30 patients (24–67 years)
+10,7%
Brightness
Fine Lines -30,2%
Wrinkles -40,4%
Enlarged pores -46,5%
Sebum -18,6%
AGENDA
1. Emerging Trend In Aesthetics: why a paradigm shift is needed
2. Introducing PRX-PLUS: Multilayer Skin Booster
3. The treatment STEP by STEP
4. Standalone & Combined Procedures
PRX-PLUS: A Three Step Needle-free Treatment
1. CLEANSE
2. APPLY
3. MOISTURIZE
with
PRX-PLUS with
WiQo Nourishing and Moisturizing
WiQo P Solution (technical massage) Cream for dry skin
PRX-PLUS Application Massage Protocol – Key Guidelines
The application of PRX-PLUS is performed through a specific massage technique that requires
precision and adherence to protocol in order to maximize efficacy and ensure optimal results
1. Glove Selection:
Always use nitrile gloves, not latex, to prevent allergic reactions
When using a spatula (e.g., WiQo spatula), cover the tip with nitrile material for
safety and glide
2. Initial Application:
Start by applying the product on a small, limited test area to assess skin response
3. Massage Technique:
Massage the product gently into the skin
Ensure the skin is well-lubricated to avoid friction or micro-abrasions
If the glove or spatula does not glide smoothly, apply additional product
4. Sensory Feedback:
A slight tingling is normal and expected.
If the patient reports intense burning or discomfort, it may indicate skin irritation.
In this case:
Stop the procedure immediately.
Rinse with water thoroughly.
Apply WiQo Nourishing and Moisturizing Cream for Dry Skin to soothe the area.
PRX-PLUS
WARNINGS AND CONTRAINDICATIONS
WARNINGS* GENERAL CONTRAINDICATIONS
_ For external use only, topical application. / Irritated and/or irritable skin
_ Avoid contact with eyes; in case of contact, rinse with / Seborrheic or atopic dermatitis
saline solution.
/ Macerated skin, open wounds, rashes, and/or herpetic
_ If the patient is using topical retinoic acid Eruptions
(isotretinoin), discontinue its use 15 days before
applying PRX-PLUS / Epidermal and dermal tumor pathologies or other
neoplastic conditions with possible local involvement
_ Do not apply to the corners of the mouth if affected by (e.g., cutaneous lymphoma)
angular cheilitis.
/ Allergy to the product components
_ Avoid application on nevi of any histological type and/
or on skin with burns of various degrees, sunburn, etc. / Pregnancy and breastfeeding
_ Treat at a reasonable distance from the last shave or
waxing.
_ Store PRX-PLUS at 2°C/8°C, even after opening the vial.
*It is recommended to follow the instructions as per the package leaflet
PRX-PLUS: The Standalone Protocol
Full cycle*: 4 sessions (avg) 5 vials of 4ml per box
10 min (face)
Duration of the session:
15 min (face & neck)
1 ml (face)
Amount of product needed:
1,5 ml (face & neck)
Frequency of sessions: 14 days apart
2nd complete cycle: After 6 months
Approximately 20 treatments per box.
*The number of sessions can vary from 3 to 5 according to skin condition to professional evaluation.
HOME CARE
HOME CARE: COSMECEUTICALS
MOISTURIZE STIMULATION OF SKIN RENEWAL
WiQo Nourishing and Moisturizing
Cream for Dry Skin 50ml WiQo Smoothing Fluid
Shea Butter - Jojoba Oil - Wheat Germ Oil - pH ~ 3
Grape Seed Oil - Echinacea Leaf Extract GLYCOLIC ACID
_BENEFITS _BENEFITS
• PREVENTS MOISTURE LOSS • SMOOTHING
• RESTORES COMFORT AND SOFTNESS • REDUCES THE SIZE AND NUMBER OF PORES
• HELPS STRENGTHEN THE SKIN BARRIER • IMPROVES SKIN ELASTICITY
HOME CARE
BEAUTY ROUTINES
RECOMMENDED SKIN CARE DURING/AFTER THE PRX SESSION CYCLE
SKIN NEEDS... WiQo RESPONDS
WiQo Nourishing and Moisturizing Cream for Dry Skin, thanks to the
DAY NIGHT
HYDRATION presence of high-quality shea butter, restores the hydrolipidic film altered
to avoid unwanted effects caused by excessive skin dehydration
by treatment, ensures hydration and nutrition, and increases skin
elasticity..
WiQo Nourishing and Moisturizing Cream for Dry Skin
WiQo Smoothing Fluid, thanks to the presence of a low concentration
STIMULATION OF SKIN RENEWAL NIGHT
of glycolic acid with a pH lower than that of the skin, stimulates the
to enhance the beneficial effects induced by professional
treatment skin, improves its firmness and prolongs skin smoothness and
luminosity.
WiQo Smoothing Fluid
AGENDA
1. Emerging Trend In Aesthetics: why a paradigm shift is needed
2. Introducing PRX-PLUS: Multilayer Skin Booster
3. The treatment STEP by STEP
4. Standalone & Combined Procedures
PRX PLUS: BEFORE AND AFTER 3 SESSIONS
PRX PLUS: BEFORE AND AFTER 3 SESSIONS
PRX PLUS: BEFORE AND AFTER 3 SESSIONS
PRX PLUS: BEFORE AND AFTER 3 SESSIONS
PRX PLUS
COMBINED TREATMENTS
ADVANCED COMBINATIONS
PRX-PLUS enhances:
/ INJECTABLES / EBD (NON ABLATIVE)
/ MICRONEEDLING / EBD (ABLATIVE)
Combined procedure
PRX-PLUS + injectable skin booster
Technique: Depending on the injectable skin booster
- First, apply the biorevitalizer, then PRX-PLUS with a
gentle massage, avoiding direct contact with the injection points.
Or
-First apply PRX PLUS and then inject the injectable skin
booster.
Synergy: Combines chemical biorevitalization with injectable
collagen stimulation to improve firmness, elasticity, and dermal
remodeling.
Combined procedure
PRX-PLUS + MICRONEEDLING
Technique: First, apply PRX-PLUS with a massage (2 layers), perform
microneedling, and then apply PRX-PLUS with a gentle massage (1
layer) to enhance absorption and dermal stimulation.
Synergy: Combines mechanical collagen induction with chemical
biorevitalization to maximize fibroblast activation and skin renewal.
Combined procedure
PRX-PLUS + Radiofrequency
Technique: Perform radiofrequency treatment first to induce
controlled dermal heating; then apply PRX-PLUS with gentle massage
to optimize biorevitalization and collagen remodeling.
Synergy: Combines thermal collagen contraction with chemical
stimulation to improve skin firmness, texture, and density.
Combined procedure
PRX-PLUS + Ultherapy®
Technique: Perform Ultherapy® first to stimulate the deep layers
with focused ultrasound energy; then apply PRX-PLUS with a gentle
massage to promote surface renewal and hydration.
Synergy: Enhances neocollagenesis and skin tightening by combining
deep stimulation with chemical biorevitalization, resulting in firmer,
lifted, and more radiant skin.
Combined procedure
PRX-PLUS as Skin Preparation Prior to
Ablative Laser Treatment
Technique: Perform 1–2 PRX-PLUS sessions in the weeks prior to ablative laser treatment to improve skin hydration,
elasticity, and barrier function, optimizing tolerance and recovery.
Synergy: Skin preconditioning with PRX-PLUS increases fibroblast activity and dermal quality, promoting faster healing,
uniform re-epithelialization, and improved post-laser results.
Combined procedure
PRX-PLUS + Ablative Laser
Technique: Perform ablative laser treatment (CO₂ or Er:YAG) first to remove damaged epidermal layers, and then apply
PRX-PLUS once re-epithelialization has begun to optimize collagen remodeling and recovery.
Synergy: Combines fractional ablation with chemical biorevitalization to accelerate regeneration, improve texture, and
enhance skin radiance.
BENEFITS FOR THE DOCTOR
• Delegable and cost-effective procedure
• High patient satisfaction
• No risk of PIH or peeling
• Compatible with all skin types
CONCLUSIONS
• PRX-PLUS redefines prejuvenation:
• Regeneration without damage or needles;
• Deep stimulation, natural effect;
• Ideal as a base or complement;
• Regenerated beauty from the surface.
CLINICAL Q&A:
ADVANCED USE OF PRX-PLUS
Clinical Q&A
PRX-PLUS in Advanced Clinical Practice
/ Evidence-based answers to the most frequent clinical questions
/ Indications, protocols, and combination strategies
/ Post-procedure recovery and inflammation control
/ Safe use across skin types and technologies
PRX-PLUS After Acne Scar Treatments
Indication: Post-procedural erythema & regeneration
/ Effective after:
- Microneedling
- RF Microneedling
- Fractional non-ablative lasers
/ Reduces post-inflammatory erythema
/ Enhances collagen remodeling without epidermal damage
Protocol
/ Apply 7–14 days post-procedure (after re-epithelialization)
/ 1–2 layers with gentle massage
/ Repeat every 3–4 weeks if needed
PRX-PLUS & Corticosteroid Withdrawal
Can PRX-PLUS be used? Yes — with caution
/ Supports dermal repair and barrier restoration
/ Indicated only after acute inflammation stabilizes
Clinical Criteria Before Treatment
/ No oozing, crusting, or erosion
/ Burning sensation resolved
/ Skin barrier function restored
Timing After Steroid Suspension
When to safely introduce PRX-PLUS
/ Minimum waiting period: 2-4 weeks after steroid discontinuation
/ Final decision based on:
- Skin tolerance
- Barrier integrity
- Absence of dermatitis
Protocol
/ Start with 1 light layer
/ Test spot recommended
/ Sessions every 7–10 days, 3–5 total
Does PRX-PLUS Cause Peeling?
Understanding epidermal response
/ PRX-PLUS is not a traditional exfoliative peel
/ Epidermis remains intact
Possible reactions
/ Mild dryness or micro-flaking
/ No sheet peeling
/ Minimal social downtime
Duration of Peeling (If Present)
/ Typically, 24–72 hours
/ Rarely exceeds 3–4 days
/ Easily controlled with moisturization
/ Does not interfere with normal activities
PRX-PLUS with High-Tech Devices
Same-session strategies
Correct sequence
1. Energy-based treatment (RF, laser, ultrasound)
2. Skin cooling and stabilization
3. PRX-PLUS application (1–2 layers)
4. Barrier repair + SPF
Clinical benefits
/ Faster recovery
/ Reduced erythema
/ Enhanced collagen response
PRX-PLUS + CFU Devices
Optimizing ultrasound outcomes
/ CFU targets deep dermis without epidermal injury
/ PRX-PLUS enhances surface regeneration and hydration
Protocol
/ Same day or within 3–7 days post-CFU
/ No additional downtime
/ Normal activity same day
Treatment cadence
/ CFU: every 6–12 months
/ PRX-PLUS: series of 3–5 sessions
PRX-PLUS + Microneedling
Two safe clinical approaches
Option A — Same day
/ Perform microneedling
/ Wait 10–15 minutes
/ Apply PRX-PLUS gently (light pressure)
Option B — Staged
/ Apply PRX-PLUS 5–7 days post-procedure
Avoid immediate application on deep RF microneedling channels
PRX-PLUS + CO₂ / Ablative Lasers
Three safe clinical approaches
Option A — Pre Treatment
/ Apply PRX-PLUS
/ Wait 10-14 days
/ Perform laser (Energy/density according to skin type)
Option B — Same day
/ Perform laser (low energy/low density)
/ Wait 10–15 minutes
/ Apply PRX-PLUS gently (light pressure)
PRX-PLUS + CO₂ / Ablative Lasers
Three safe clinical approaches
Option C — After Treatment
/ Perform laser (Energy/density according to skin type)
/ Wait for full re-epithelialization (10–14 days)
/ Use PRX-PLUS as:
- Recovery accelerator
- Redness reducer
- Collagen remodeling booster
Frequency
/ Every 3–4 weeks post-laser
Safety & Skin Type Compatibility
Clinical safety profile
/ Suitable for Fitzpatrick I–VI
/ Safe year-round
/ Minimal PIH risk when protocol is followed
/ Epidermis preserved
Common transient reactions
/ Mild erythema
/ Tingling sensation
/ Temporary dryness
Key Take-Home Message
Why PRX-PLUS fits modern aesthetics
/ Regeneration without needles or damage
/ Ideal bridge between devices and recovery
/ Enhances outcomes of advanced procedures
/ High satisfaction, minimal downtime
PRX-PLUS supports skin quality first — structure follows.
THANK YOU
QUESTIONS?
