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BIOENG472 TOPIC9 LiquidBiopsies2 Exosomes

Exosomes are 30-100 nm microvesicles shed by cells that contain cytosolic components like DNA, RNA, and proteins. They can be found in many bodily fluids and provide information about cells and tissues through a liquid biopsy. Researchers have developed several microfluidic techniques to separate and analyze exosomes from small sample volumes in order to diagnose and monitor cancers and other diseases.

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53 views9 pages

BIOENG472 TOPIC9 LiquidBiopsies2 Exosomes

Exosomes are 30-100 nm microvesicles shed by cells that contain cytosolic components like DNA, RNA, and proteins. They can be found in many bodily fluids and provide information about cells and tissues through a liquid biopsy. Researchers have developed several microfluidic techniques to separate and analyze exosomes from small sample volumes in order to diagnose and monitor cancers and other diseases.

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Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Liquid Biopsies (2):

Exosomes
Exosomes are 30-100 nm microvesicles shed by all cells

• First described in the 1980s


• Both healthy and tumor cells shed exosomes due to unknown reasons
 Contain a sample of cytosolic milieu (DNA, RNA, proteins) but have a different lipid content
 Exosomes are found in most bodily fluids (blood, urine, saliva, breast milk)
 Exosomes can report the presence/identity of a tumor from a “liquid biopsy”
Iero et al., “Tumour-released exosomes and their implications in cancer
immunity”, Cell Death and Differentiation 15: 80 (2008)
The role of exosomes in cancer
The role of exosomes in promoting metastasis

Martins et al., “Tumor-cell-derived microvesicles as carriers of molecular


information in cancer”, Current Opinion Oncology 25: 66 (2013)
Microvortex exosome separation

Chen et al. (Irimia’s group), Lab Chip 10: 505 (2010)


Filtration systems to capture exosomes

R. T. Davies, J. Kim, S. C. Jang, E.-J. Choi, Y. S. Gho and J. Park, Lab Chip 12: 5202 (2012)
Filtration systems to capture exosomes

 Poor retention of larger particles and proteins

Z. Wang, H.-J. Wu, D. Fine, J. Schmulen, Y. Hu, B. Godin, J. X. J. Zhang and X. Liu, Lab Chip 13:
2879 (2013)
Microfluidic separation of exosomes using immunomagnetic beads

Zhao et al., “A microfluidic ExoSearch chip for multiplexed exosome detection


towards blood-based ovarian cancer diagnosis”, Lab Chip 16: 489 (2016)
Integrated microfluidic exosome analysis directly from human plasma

He et al., “Integrated immunoisolation and protein analysis of circulating exosomes


using microfluidic technology”, Lab Chip 14: 3773 (2014)

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