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Study of Diuretic Activity of Drug Using Rat/Mice: MIT-WPU - School of Pharmacy

This document provides instructions for studying the diuretic activity of drugs using rats or mice. It describes using metabolic cages to separately collect urine and feces from rats. The rats will be divided into groups receiving normal saline, saline with urea, saline with hydroflumethiazide, or saline with furosemide. Urine output will be measured over time for each group and compared to determine the diuretic activity of the drugs after 1 hour. Short questions are also provided about defining diuretics, classifying them with examples, writing adverse effects, and the mode of action of loop diuretics.

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6K views9 pages

Study of Diuretic Activity of Drug Using Rat/Mice: MIT-WPU - School of Pharmacy

This document provides instructions for studying the diuretic activity of drugs using rats or mice. It describes using metabolic cages to separately collect urine and feces from rats. The rats will be divided into groups receiving normal saline, saline with urea, saline with hydroflumethiazide, or saline with furosemide. Urine output will be measured over time for each group and compared to determine the diuretic activity of the drugs after 1 hour. Short questions are also provided about defining diuretics, classifying them with examples, writing adverse effects, and the mode of action of loop diuretics.

Uploaded by

Vidhi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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MIT-WPU | School of Pharmacy

WORLD’S FIRST UNIVERSITY


FOR LIFE TRANSFORMATION

4. STUDY OF DIURETIC ACTIVITY OF DRUG USING RAT/MICE

Dr. Chinmay Deshmukh


Assistant Professor
Email: [email protected]
Reference books
1. A Practical book of Pharmacology II by Dr. Pankaj M. Choudhari, Dr. Dheeraj T. Baviskar and Dr. Prakash

Patil, PV books, S Vikas and company (medical publishers) Jalandhar, 2019.

2. Ghosh M.N., Fundamentals Of Experimental Pharmacology, 3 rd Edition.

3. Kulkarni S.K., Practical Pharmacology And Clinical Pharmacy, Vallabh Publication,.

4. A practical book of Pharmacology-II by Hemant Suryawanshi, Mukesh Patel and Sunil Pawar, Nirali

Prakashan, Second edition, January 2020.

5. X-cology software CD for demonstration


Requirements
• Metabolic cages, graduated measuring cylinders.
• Drugs: Normal saline (0.9%), urea (900 mg/kg oral) hydroflumethiazide (1mg/kg, oral)
Frusemide (5 mg/kg oral)
• Animal: Rats
• Theory
• Diuretics are the compounds which increase the flow of urine. They are used in various
diseases such as cardiac failure, liver cirrhosis, hypertension, water poisoning and certain
kidney diseases. They are acting by increasing reabsorption of Na and Water through PCT and
DCT
• Adverse effects
• Hypovolemia, hypokalemia, hyperkalemia, hyponatremia, metabolic alkalosis, metabolic
acidosis, and hyperuricemia
• Principle

• Normal urine output in rats is 1-2 ml/rat/day.


Hence to get measurable quantity of urine, the
rats are first hydrated.

• The urine output is increased after


administration of diuretics like urea,
hydroflumethiazide and frusemide.

• This increase in urine output is measured in


measuring cylinder and compared with normal

urine output.
• Metabolic cage
• These well-engineered Metabolic & Diuresis Cages supply
uncontaminated samples of effectively separate urine and feces
of the rodent.
• Metabolic cages give an ideal separation of water through a
special style of the funnel and of the separation cone.
• Animal waste is directed through a collection funnel and onto a
linear diffuser.
• The diffuser allows solid matter to travel down the top ridges of
a 50% incline, passing over a urine port, and into a fecal
collection vessel.
• Liquid waste flows along grooves down the inclined diffuser,
passing through the urine port, and into a urine collection
vessel.
• A urine deflector prevents excess urine from splashing and
contaminating fecal specimen. Separate urine and feces samples
are collected in two standard 50 ml. centrifuge vessels.
Groups

• Group 1= Normal saline

• Group 2=Saline + urea (900 mg/kg, oral)

• Group 3=Saline + Hydroflumethaizide (1 mg/kg, oral)

• Group 4= Saline+ Furosemid (5 mg/kg oral)


Amount of urine Groups 1 2 3 4
collected in ml After 15 min

After 30 min

After 1 hour

Total volume

• Result: The diuretic activity of drugs after 1 hour are found as under
• Normal Saline =
• Saline + urea (900 mg/kg, oral)=
• Saline + hydroflumethaizide (1 mg/kg, oral)=
• Saline+ furosemid (5 mg/kg oral)=
Short Questions

1. Define diuretics and write its uses.

2.   Classify diuretics with examples.

3.    Write adverse effect of diuretics

4.  Write mode of action of loop diuretics


THANK YOU
S. No.124, Paud Road, Kothrud, Pune,
411038

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