Histology spotters examination ½ + ½ = 1M

1a Name the slide focussed under microscope T.S. of Compact bone

1) Haversian canal with vessels & nerves Osteon/
2) Lacunae with osteocytes Haversian
1b Write two identification points system
3) Concentric lamellae
 Histology spotters examination ½ + ½ = 1M
1a Name the slide focussed under microscope L.S. of Compact bone
1) Haversian canals connected by Volkman’s canal
1b Write two identification points
2) Lacunae with osteocytes
 Histology Discussion examination 1x10 = 10 M
Questions
1a Focus the given slide under microscope
1b Identify the slide focussed under microscope
1c Draw a neat diagram & label it
1d Get ready to answer questions asked by the examiner
Microscopic pictures our histology slides
T.S. of Compact bone with labelling

Concentric lamellae

Volkman’s canal
Osteon/Haversian system
Lacunae with
1. Haversian canal
osteocytes
2. Concentric lamellae
3. Lacunae with osteocytes

Haversian canal
?
 Hand diagram for Theory & Practical

Transverse section
of
Compact bone

HINT:
Draw both diagrams with dark black colour pencil
& Back ground should be light yellow colour pencil
 L.S. of Compact bone with labelling

Lacunae with osteocytes

Haversian canal

Concentric
lamellae

1. Haversian canals connected

by Volkmann’s canals
2. Lacunae with osteocytes
?
 Hand diagram for Theory & Practical

Longitudinal section of Compact bone

HINT:
Draw both diagrams with dark black colour pencil
& Back ground should be light yellow colour pencil
 Histology discussion slides examination
[Link] Questions Answers
1 Why it is Bone ? Presence of Osteocytes
2 Why it is Compact bone ? Presence of Haversian system/Osteon
3 1) Haversian canal with vessels & nerves
2) Lacunae with canaliculi contain
What are Components of Haversian system ?
Osteocytes with protoplasmic processes
3) Concentric lamellae
4 Name the outer covering of bone ? Periosteum
5 Outer fibrous layer
What are the layers of periosteum ?
Inner cellular layer
6 Fibrous layer made up of ? Dense regular C.T(Collagen fibres)
7 Function of fibrous layer Protection & nutrition
8 Function of cellular layer Appositional growth of bone
9 Cellular layer made up of ? Osteoprogenitor cells & osteoblasts
[Link] Questions Answers
10 Haversian canal
What are the Components of Canalicular Volkman’s canal
system of bone ?
Lacunae with canaliculi
11 1)Passage of blood vessels &
What is the Purpose of canalicular system ?
2)diffusion of nutrients
12 matrix will not allow diffusion of nutrients because
Why canalicular system is present? it is mineralised
13 Cortex of shaft of long bones
Location/Examples/distribution/areas where
compact bone is present ? Outer & Inner tables of Flat bone
Outer shell of Short bone
14 Osteo-progenitor cells
Osteocytes
What are the types of Cells in bone? Osteoblasts
Osteoclasts
15 Name the Multinucleated giant cell ? Osteoclast (↓ Parat hormone)
16 Name the Bone forming cells ? Osteoblasts (↓ Thyrocalcitonin)
17 Name the bone destroying cells(bone
Osteoclasts
resorption) ?
[Link] Questions Answers
Shaft of Long bone
Location/Examples/distribution/
8 Outer & Inner tables of Flat bone
areas where compact bone is present ?
Outer shell of Short bone
Osteo-progenitor cells
Osteocytes
9 What are the types of Cells ?
Osteoblasts
Osteoclasts
10 Name the Multinucleated giant cell ? Osteoclast (↓ Parat hormone)
11 Name the Bone forming cells ? Osteoblasts (↓ Thyrocalcitonin)
12 Name the bone destroying cells ? Osteoclasts
Circumferential
13 What are the types of lamellae ? Concentric lamellae
Interstitial lamellae
14 Composition of lamellae? Mineralised matrix (organic+inorganic)
15 Composition of cement lines? Inorganic matrix
[Link] Questions Answers
16 Enzyme responsible for mineralization of matrix Alkaline phosphatase
17 Immigrant cell of bone Osteoclast
18 Fixed macrophage of bone Osteoclast
19 Type of collagen fibres Type 1 Collagen fibres
20 How do you differentiate bone from cartilage ? Mineralised matrix
21 How do you differentiate compact from sponge
Presence of haversian system/osteon
bone
 Differences b/w Compact & Cancellous
Feature Compact Cancellous

Lamellae Haversian system No Haversian system

Solid bone Abundant Sparse
Spaces in the bone Small Large

Ends of Long bones

Shaft of Long bones Diploe of Flat bones
Examples
Covering of spongy bone Lining of compact bone
 Differences b/w Compact & Cancellous
Feature Cartilage Bone
Consistency Soft, flexible Sclerous C.T Hard, rigid, Sclerous C.T
Covering Perichondrium Periosteum
Cells Chondrocytes Osteocytes
without protoplasmic processes with protoplasmic processes
Canalicular system Absent Present (Haversian & Volkman’s canals)
Lacunae Without canaliculi With canaliculi
Matrix Non-mineralised Mineralized (Calcium & Phosphorus)
Collagen fibres Type 1 & Type 2 Only Type 1
Vascularity Avascular Highly vascular
Bone marrow Absent Present
Growth Bi-directional Uni-directional
Regeneration Poor Rich
Examples Support & Flexibility to the body Skeletal Support to the body