Mitosis Gives a Brief Window of Opportunity for a Change in Gene Transcription
Figure 6
Most mitotic histone modifications do not distinguish mitotic chromatin from interphase nuclei.
(a) Western blot showing TSA inhibition of the normal mitotic deacetylation of histone H3K9ac. (b) Western blot showing that AT2983 prevents the normal mitotic phosphorylation of histone H3. (c) Mitotic nuclei prepared in the presence of TSA maintain the mitotic advantage. Supporting data can be found in Data S1. (d) Inhibiting normal mitotic phosphorylation by AT2983 has no adverse effect on mitotic advantage. Supporting data can be found in Data S1. (e) MTA inhibits mitotic methylation of H3K4me3 and H3K9me3, both of which are normally increased at the Sox2 locus in C2C12 cells. This does not change the mitotic advantage. Supporting data can be found in Data S1.
doi: https://doi.org/10.1371/journal.pbio.1001914.g006