diff --git a/compile.c b/compile.c
index c0cc0be873ef05..7c51a4f134b745 100644
--- a/compile.c
+++ b/compile.c
@@ -2018,6 +2018,7 @@ iseq_set_sequence(rb_iseq_t *iseq, LINK_ANCHOR *const anchor)
 			    rb_hash_rehash(map);
 			    freeze_hide_obj(map);
 			    generated_iseq[code_index + 1 + j] = map;
+			    FL_SET(iseq, ISEQ_MARKABLE_ISEQ);
 			    break;
 			}
 		      case TS_LINDEX:
@@ -2028,6 +2029,9 @@ iseq_set_sequence(rb_iseq_t *iseq, LINK_ANCHOR *const anchor)
 			{
 			    VALUE v = operands[j];
 			    generated_iseq[code_index + 1 + j] = v;
+			    if (!SPECIAL_CONST_P(v)) {
+				FL_SET(iseq, ISEQ_MARKABLE_ISEQ);
+			    }
 			    break;
 			}
 		      case TS_VALUE:	/* VALUE */
@@ -2035,6 +2039,9 @@ iseq_set_sequence(rb_iseq_t *iseq, LINK_ANCHOR *const anchor)
 			    VALUE v = operands[j];
 			    generated_iseq[code_index + 1 + j] = v;
 			    /* to mark ruby object */
+			    if (!SPECIAL_CONST_P(v)) {
+				FL_SET(iseq, ISEQ_MARKABLE_ISEQ);
+			    }
 			    break;
 			}
 		      case TS_IC: /* inline cache */
@@ -2045,6 +2052,9 @@ iseq_set_sequence(rb_iseq_t *iseq, LINK_ANCHOR *const anchor)
 				rb_bug("iseq_set_sequence: ic_index overflow: index: %d, size: %d", ic_index, iseq->body->is_size);
 			    }
 			    generated_iseq[code_index + 1 + j] = (VALUE)ic;
+			    if (BIN(once) == insn || BIN(trace_once) == insn) {
+				FL_SET(iseq, ISEQ_MARKABLE_ISEQ);
+			    }
 			    break;
 			}
 		      case TS_CALLINFO: /* call info */
diff --git a/iseq.c b/iseq.c
index e59a612de8d635..7743cd3e8a1f58 100644
--- a/iseq.c
+++ b/iseq.c
@@ -215,7 +215,10 @@ rb_iseq_mark(const rb_iseq_t *iseq)
     if (iseq->body) {
 	const struct rb_iseq_constant_body *body = iseq->body;
 
-	rb_iseq_each_value(iseq, each_insn_value, NULL);
+	if(FL_TEST(iseq, ISEQ_MARKABLE_ISEQ)) {
+	    rb_iseq_each_value(iseq, each_insn_value, NULL);
+	}
+
 	rb_gc_mark(body->variable.coverage);
 	rb_gc_mark(body->variable.original_iseq);
 	rb_gc_mark(body->location.label);
diff --git a/iseq.h b/iseq.h
index d238a55fe4d5f8..31a3232e3be1f7 100644
--- a/iseq.h
+++ b/iseq.h
@@ -84,6 +84,7 @@ ISEQ_ORIGINAL_ISEQ_ALLOC(const rb_iseq_t *iseq, long size)
 #define ISEQ_NOT_LOADED_YET   IMEMO_FL_USER1
 #define ISEQ_USE_COMPILE_DATA IMEMO_FL_USER2
 #define ISEQ_TRANSLATED       IMEMO_FL_USER3
+#define ISEQ_MARKABLE_ISEQ    IMEMO_FL_USER4
 
 struct iseq_compile_data {
     /* GC is needed */