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Answer: emptyDrops new default alpha = Inf giving far fewer non-empty droplets than unde
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Aaron Lun
★ 29k
I suspect the real problem is that the knee point is not being identified correctly in your plot. Visually, I would put the knee point some…
Comment: heatmap with batch effect
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mike
• 0
This is a really helpful discussion about addressing batch effects in heatmap visualizations. It's insightful to see the challenges when co…
Comment: How to use limma to analyze complex temporal RNAseq data
by
Jinming Cheng
• 0
Hi Jiahao, Thanks for more details about your analysis! For my design (`~ 0 + group + time`), confounding factors or interactions were not…
Comment: How to use limma to analyze complex temporal RNAseq data
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Jiahao Tian
• 0
Hi Jinming, Great thanks for your reply! I have no doubt that `voomLmFit` handles complex temporal variations. However, I suspect that yo…
Comment: emptyDrops new default alpha = Inf giving far fewer non-empty droplets than unde
by
Peter Hickey
▴ 760
Session info ``` r > sessionInfo() R version 4.5.1 (2025-06-13) Platform: x86_64-pc-linux-gnu Running under: Red Hat Enterprise Linux 9.3 …
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Answer: Limpa Pipeline Order
Answer: Limpa Pipeline Order
Answer: Should voomWithQualityWeights still be used when the sample-specific weights var
Answer: Should voomWithQualityWeights still be used when the sample-specific weights var
Answer: makeChipPackage(): Error in data.frame(..., check.names = FALSE) : arguments
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