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Surgical Spirit: Defination

This document provides the definition, identification tests, and assay for surgical spirit. Surgical spirit contains 5 ml of methyl salicylate, 20 ml of diethyl phthalate, and 25 ml of castor oil with the remainder being industrial methylated spirit to produce 1000 ml total. Two identification tests are described - one producing a violet color with iron chloride and the other producing a green fluorescence that changes with pH. The weight per ml is specified as 0.817 to 0.827 g and the assay involves dilution and measurement of absorbance at 306 nm to calculate the methyl salicylate content.

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666 views1 page

Surgical Spirit: Defination

This document provides the definition, identification tests, and assay for surgical spirit. Surgical spirit contains 5 ml of methyl salicylate, 20 ml of diethyl phthalate, and 25 ml of castor oil with the remainder being industrial methylated spirit to produce 1000 ml total. Two identification tests are described - one producing a violet color with iron chloride and the other producing a green fluorescence that changes with pH. The weight per ml is specified as 0.817 to 0.827 g and the assay involves dilution and measurement of absorbance at 306 nm to calculate the methyl salicylate content.

Uploaded by

asit_m
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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SURGICAL SPIRIT

Defination
Methyl salicylate 5 ml (0.45 to 0.55% v/v)
Diethyl phthalate 20 ml (1.80 to 2.20% v/v)
Castor oil 25 ml
Industrial Methylated spirt sufficient to produce 1000 ml

Identification
A. To 1 ml add 0.5 ml of 0.4 M iron(III) chloride hexa hydrate. A violet color is produced.
B. To 1 ml add 50 mg of resorcinol and 1 ml of sulphuric acid , heat for 1 min, cool, pour in to
water and make alkaline with 5M sodium hydroxide. A distinct green fluorescence is
produced which disappears when the solution is made acidic and reappears when it is
made alkaline.
TESTS
Weight per ml
0.817 to 0.827 g.
Assay
For Methyl salicylate
Diute 5 ml to 100 ml with absolute ethanol, further dilute 10 ml of this solution to 100 mnl
with absolute ethanol and measure the absorbence of the resulting solution at the maximum at
306 nm, Appendix II B. Caliculate the content of C8H8O3 taking the 335 of the absorbence of a
1% v/v solution of methyl salicylate at the maximum at 306 nm a 1 cm cell.

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