Biomaterials - Tissue Interactions: 2.79J/3.96J/20.441/HST522J

The document discusses analyzing a new polymer coating containing calcium phosphate particles applied to orthopedic prostheses, and determining the presence of silica particles in breast prostheses. For the polymer coating, infrared spectroscopy or ESCA could verify if calcium phosphate particles are within 5 nm of the coating surface and measure their composition. Light microscopy alone is insufficient to check for loose particles on the surface. Contact angle measurements of the coating require information on measurement conditions and static rather than dynamic contact angles. Scanning electron microscopy or transmission electron microscopy would be appropriate to detect 2 nm silica particles in breast prosthesis samples, while x-ray diffraction alone may not be sensitive enough.

Uploaded by

Guilherme Henrique

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

0% found this document useful (0 votes)

34 views

Biomaterials - Tissue Interactions: 2.79J/3.96J/20.441/HST522J

Uploaded by

Guilherme Henrique

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

You are on page 1/ 2

2.79J/3.96J/20.

441/HST522J

Biomaterials - Tissue Interactions

Homework #5
1. A new company, Polycoat, offers a service to orthopedic companies in which they apply a
polymer coating, 100 nm thick, to cobalt-chromium alloy prostheses. The coating contains
calcium phosphate particles, 10 nm in diameter. The putative benefits of the coating are: 1) bone
will bond to it and 2) water absorbed by the polymer will slowly dissolve the particles thereby
releasing calcium, which will favorably affect nearby osteoblasts. You have been hired by an
orthopedic company to help them analyze Polycoat-coated prostheses and review data from
Polycoat.
a. It is important to verify that the calcium phosphate particles are within 5 nm of the
surface of the Polycoat coating. Given that you can employ only one of the following
methods which would you chose and what would you measure: infrared spectroscopy,
ESCA, X-ray diffraction? Explain.
b. Polycoat states that there should be no concern about loose calcium phosphate particles
on the surface of the coating because their conventional light microscopy studies have
not revealed particles on the surface. Is there anything that you would recommend
relative to this issue?
c. In reference to the hydrophobic-hydrophilic character of the coating, Polycoat
determined the contract angle of water on the surface of the coating. What information
would you request about the conditions under which the contact angle measurement was
made? What type of contact angle measurement would you require for this coating?
2. The silicone elastomer used to fabricate breast prostheses is normally filled with
noncrystalline silica particles, 2 nm in diameter, to give it strength. A question has been raised
about whether such particles are actually in a sample of the prosthesis given to you for study.
Comment about the appropriateness of each of the following methods for determining if such
silica particles are in your sample.
a.
Scanning electron microscopy
b.
Transmission electron microscopy
c.
X-ray diffraction