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Buffer Solutions Preparation Guide

The document describes how to prepare different buffer solutions with specific pH values. It provides instructions for making acetate, carbonate/bicarbonate, citric acid, phosphate, TRIS, barbitone, cacodylate, and phthalate buffers by mixing appropriate volumes of acid and conjugate base solutions and diluting to a final volume. The pH values obtained by adding different volumes of acid are also shown for each buffer system.

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235 views1 page

Buffer Solutions Preparation Guide

The document describes how to prepare different buffer solutions with specific pH values. It provides instructions for making acetate, carbonate/bicarbonate, citric acid, phosphate, TRIS, barbitone, cacodylate, and phthalate buffers by mixing appropriate volumes of acid and conjugate base solutions and diluting to a final volume. The pH values obtained by adding different volumes of acid are also shown for each buffer system.

Uploaded by

tushar84
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Buffer Solutions Composition:-

A. Acetate Buffer (0.2 mol/liter):-


Place x ml of 0.2 mol/liter acetic acid in a 100 ml volumetric flask and make up top the mark with
0.2 mol/liter sodium acetate.
X ml 92 88 83 76 66 55 43 32 22 17 7
pH 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 5.2 5.4 5.6

B. Carbonate/ Bicarbonate Buffers (0.1 mol/liter):-


Place x ml of 0.1 mol/liter Sodium Bicarbonate into a 100 ml volumetric flask and make up top the
mark with 0.2 mol/liter Sodium Carbonate.
X ml 93 88 82 73 61 49 38 27 18 10 5.5
pH 9.0 9.2 9.4 9.6 9.8 10.0 10.2 10.4 10.6 10.8 11.0

C. Citric Acid/ Sodium Citrate Buffers(0.05 mol/liter):-


Place x ml of 0.05 mol/liter Citric acid in a volumetric flask and make up top the mark with 0.05
mol/liter Trisodium citrate.
X ml 91 86 80 75 70 65 60 55 50 44 39
pH 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0
X ml 34 29 24 19 14
pH 5.2 5.4 5.6 5.8 6.0

D. Phosphate Buffers (0.1 mol/liter):-


a. Sodium Phosphate Buffers:-
Place x ml of 0.2 mol/liter Sodium Hydroxide to 50 ml of 0.2 mol/liter Sodium dihydrogen
phosphate and dilute to 100 ml.
X ml 3.5 5.8 9.1 13 18 24 30 35 40 43 45 47
pH 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0

b. Potassium Phosphate Buffer:-


Place x ml of 0.2 mol/liter Potassium Hydroxide to 50 ml of 0.2 mol/liter Potassium dihydrogen
phosphate and dilute to 100 ml.
X ml 3.5 5.8 9.1 13 18 24 30 35 40 43 45 47
pH 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0

E. TRIS (Hydroxymethyl) Amino Methane/ HCL Buffer (0.1mol/liter):-


Place x ml of 0.2mol/liter Hydrochloric acid to 50 ml of 0.2 mol/liter tris and make up to 100 ml
with water.
X ml 43 41 39 34 29 24 18 13 9.5 6.0
pH 7.2 7.4 7.6 7.8 8.0 8.2 8.4 8.6 8.8 9.0

F. Barbitone Buffer (0.07 mol/liter, pH 8.6):-


Dissolve 2.58g of diethylbarbituric acid and 14.42g of sodium diethylbarbiturate in water and make
up to 1 liter.

G. Cacodylate Buffer (0.1 mol/ liter, pH 6.5):-


Add 29 ml of 0.2 mol/liter HCl to 50 ml of Sodium Cacodylate and make up to 100 ml with water.

H. Cacodylate Buffer (0.1 mol/ liter, pH 6.5):-


Add 16 ml of 0.2 mol/liter HCl to 50 ml of Sodium Cacodylate and make up to 100 ml with water.

I. Phthalate Buffer (0.1 mol/liter, pH 6.0):-


Add 43 ml of 0.1 mol/liter NaOH to 50 ml of 0.2 mol/liter potassium hydrogen phthalate.

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