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Diabetes Detection with Benedict’s Test

Benedict's test is used to detect glucose in urine by heating urine with a copper sulfate solution. If glucose is present, the solution will change from blue to various shades of green, yellow, or red, indicating the amount of glucose detected. The test is used to monitor diabetes and the effectiveness of treatment by testing urine before meals and observing any color change when mixed with Benedict's solution and heated. Heating provides energy for the reaction between glucose and the indicator to occur, with different colors corresponding to varying levels of detected glucose.

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1K views1 page

Diabetes Detection with Benedict’s Test

Benedict's test is used to detect glucose in urine by heating urine with a copper sulfate solution. If glucose is present, the solution will change from blue to various shades of green, yellow, or red, indicating the amount of glucose detected. The test is used to monitor diabetes and the effectiveness of treatment by testing urine before meals and observing any color change when mixed with Benedict's solution and heated. Heating provides energy for the reaction between glucose and the indicator to occur, with different colors corresponding to varying levels of detected glucose.

Uploaded by

San Dy
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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BENEDICTS TEST

DEFINITION: A test for detecting glucose in urine in which urine is heated with a blue solution containing sodium carbonate, sodium citrate, and copper sulfate, producing a red, yellow, or orange precipitate in the presence of glucose. Purposes: 1. Gauges the amount of sugar present in the urine.

2. To monitor the effectiveness of medication and other methods used to treat patients from illnesses arising from the detection of glucose in the urine. 3. To confirm diagnosis of diabetes (gestational diabetes in pregnant women).

PROCEDURE 1. Collect urine specimen before meals. 2. Put 5 mL of Benedicts solution into the test tube and heat it. 3. Note for the color changes. 4. Add 8-10 drops of urine into the test tube with 5 mL Benedicts solution. 5. Heat the solution but do not boil. 6. Note and record for color changes.

RATIONALE For greater accuracy Heating will provide the energy for the reaction with Benedicts indicator to occur. Color indicates how much glucose is present. Presence of glucose ranges from green (least) to red (abundant). Blue indicates no glucose is present. To verify the 1st finding. Heating is enough to form a reaction. Color indicates how much glucose is present.

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