Scribd
Upload
387 views10 pages

Stool Culture Lab 150

The document provides instructions for performing a stool culture to identify various bacterial pathogens that may be present in a stool sample. It lists the different selective media that should be used, such as MacConkey agar, XLD agar, EMB agar, TCBS agar, and campylobacter selective media. The document also provides information to help identify common stool pathogens like E. coli, Salmonella, Shigella, Campylobacter, and Vibrio cholerae based on their growth characteristics on different culture media.

Uploaded by

api-3699361
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
387 views10 pages

Stool Culture Lab 150

The document provides instructions for performing a stool culture to identify various bacterial pathogens that may be present in a stool sample. It lists the different selective media that should be used, such as MacConkey agar, XLD agar, EMB agar, TCBS agar, and campylobacter selective media. The document also provides information to help identify common stool pathogens like E. coli, Salmonella, Shigella, Campylobacter, and Vibrio cholerae based on their growth characteristics on different culture media.

Uploaded by

api-3699361
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
You are on page 1/ 10

Stool Culture

all thing in this color is done by me


‫وعين الصقر ميزانة‬
‫و سبحان من ل يخطأ‬
Stool or Rectal swab culture
*macConky plate (NLF)
*XLD agar (shigila→???/ salmonila→black)
*EMB agar → E - mythylen blue
*HE
*TCBS (y.colores) → vribo & cholera → yellow color
*alkaline peptone water (APW) → cholera
*selenite F broth
*selective media for campylobactor
(skirrow &blaser with suplements)
campylobactor microaerophilic (42C-O2 5%-
Co210%-N2 85%-48hr)
selenite F broth

For salmonila
suspectef organisms

1- E-coli (infant &NLF) it not a normal flora in


infant

2- salmonella & shigella


3-campylobactor
jejuni most common human
pathogen indol&citrate (+)
urease (- )
oxidase (+)
nalidixic acid (S) s=
sensitive

cephalo thin (R) r/


campylobactor
4-V.cholerae
serogroup -01
catalse (+)
oxidase (+)
motile (+)
lactose (NLF)
sensitive to (dry – sunlight – acid pH)
TCBS (thiosulfate citrate
bile sucrose agar –yallow colonies)
Vibrio cholerae TCBS
The End

You might also like