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Buffer Recipes: Name Description Recipe

This document provides recipes for various buffers used in biological research. It lists the name, description, and ingredients to make Tris buffer, DMEM buffer, Leibovitz's L-15 medium, PBS buffer, RIPA lysis buffer, SDS-PAGE running buffer, TAE buffer, TBS buffer, Western transfer buffer, and structures of common compounds used in buffers like Tris, EDTA, SDS, NP-40, sodium deoxycholate, and Triton X-100. The buffers are used for applications like making collagen matrices, cell culture media, lysis of cells, electrophoresis, and protein transfer.

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pisces7390
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39 views3 pages

Buffer Recipes: Name Description Recipe

This document provides recipes for various buffers used in biological research. It lists the name, description, and ingredients to make Tris buffer, DMEM buffer, Leibovitz's L-15 medium, PBS buffer, RIPA lysis buffer, SDS-PAGE running buffer, TAE buffer, TBS buffer, Western transfer buffer, and structures of common compounds used in buffers like Tris, EDTA, SDS, NP-40, sodium deoxycholate, and Triton X-100. The buffers are used for applications like making collagen matrices, cell culture media, lysis of cells, electrophoresis, and protein transfer.

Uploaded by

pisces7390
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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http://www.geocities.com/simonwaynemoore/protocols.

htm Page 1 of 3
Buffer Recipes
Name Description Recipe
Balanced
Salt
50mM Tris, pH 7.5
150mM a!l
For 1L:
" #.05g Tris
" $00ml %H&'
" $.#7g a!l
" pH to 7.5
" (il)te to 1*
DMEM (10X)
" Ma%e from pow%er
" +se% for ma,ing collagen
matrices for e-plant c)lt)res
For a detailed description see
media formulations
" $0ml of %H&'
" .%% (M/M pow%er 01igma 2(7777"1*3
" Mi- thoro)ghly
" pH to 1.$ with 10M H!l 04&0 %rops3
" Mi- )ntil completely %issol5e%
" 6ring to 100ml with %H&'
" 1yringe filter 7 ali8)ot
Leibovitz's L-15 Medim
Invitrogen #
41300-039 (powder)
1141-0!4 (li"uid)
#arious vitamins$ salts and
amino acids
For a detailed description see
media formulations
For 5L !rom po"der:
" 9inse o)t glass :ottles with water 0no soap3
" .)tocla5e
" ;n a 5* :ea,er, a%% <* %istille% water
" (issol5e 5 pac,s of pow%er
" pH to 7.# with H!l
" 6ring to 5* with %istille% water
" =ilter sterili>e into a)tocla5e% :ottles )n%er sterile con%itions
#BS
(%&osp&ate 'uffered saline)
pH 7.<
137mM a!l
&.7mM ?!l
<.3mM a&HP'<
1.<mM ?H&P'<
For 1L o! 1$%:
" $0g a!l
" &g ?!l
" 1<.< a&HP'<
" &.<g ?H&P'<
" pH to 7.<
N#-&$ l'sis b!!er
10mM Tris, pH 7.5
150mM a!l
1@ P"<0
For 5$ml:
" 500l of 1M Tris, pH 7.5
" <0.75ml of %H&'
" 5ml 10@ P"<0
" 3.75ml of &M a!l
R(#) l'sis b!!er
((adio Immuno %recipitation
)ssa* l*sis 'uffer)
50mM Tris, pH 7.5
or
10mM Phosphate, pH 7.5A
150mM a!l
+etergents,
1@ P"<0
0.1@ 1(1
0.5@ (eo-ycholate
For 5$ml:
" &.5ml of 1M Tris, pH 7.5
or &.5ml of &00mM aP, pH 7.5A
" 35.75 of %H&'
" 3.75ml of &M a!l
" 5ml of 10@ P"<0
" &.5ml of 10@ so%i)m %eo-ycholate
" 500Bl of 10@ 1(1
" 6ring to 50ml
- %&osp&ate is a useful 'uffer 'ecause it in&i'its
p&osp&atases$ &owever it will precipitate in t&e presence of
calcium or manganese.
SDS-#)*E
Rnnin+ B!!er (10X)
/&en diluted to 1X,
0m1 2ris
13 4+4
000m1 5l*cine
For 1L o! 1$%:
" 30.3g T9;1
" 10g 1(1
" 1<<g Clycine
,$$mM Sodim #-osp-ate
For 5$ml:
" 1.<1g 1o%i)m Phosphate
" <0ml %H&'
" pH to 7.<
" 6ring to 50ml
5$% .)E
(2ris acetate 6+2))
/&en diluted to 1X,
0.04 1 2ris - )cetate
0.001 1 6+2)
For 5$$ml:
" 1&1g Tris 6ase
" &$.#ml glacial acetic aci%
" D.3g /(T. 0or 0ml of 0.1 6+2))
" %H&' to 500ml
1M .ris
For 5$ml:
" #.0#g Tris
" <0ml %H&'
" pH to 7.5 with E5ml 10M H!l
" 6ring to 50ml
.BS
(2ris 'uffered saline)
50mM Tris
137mM a!l
&.7mM ?!l
For 1L:
" $00ml %H&'
" 1#g a!l (or !7.ml of 01 8a9l)
" 0.&g ?!l
" 50ml of 1M Tris, pH 7.5 (see a'ove)
" 6ring to 1* with %H&'
/estern
.rans!er B!!er (10X)
/&en diluted to 1X,
&0mM Tris
150mM Clycine
For 1L o! 1$%:
" &<.&g T9;1
" 11&.#g Clycine
http://www.geocities.com/simonwaynemoore/protocols.htm Page & of 3
Strctres
.ris ."een-,$
ED.)
SDS
(4odium +odec*l 4ulfate)
N#-&$
(Nonidet P 40$ I56%): 9)-!30 or
Nonylphenyl-polyethylenglycol)
Sodim Deo%'c-olate
nF 45
.riton 0-1$$
http://www.geocities.com/simonwaynemoore/protocols.htm Page 3 of 3

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