TOTAL-IRON BINDING PROCEDURE QUALITY CONTROL

For accuracy and reproducibility control:-

CAPACITY Pipette into 10 ml centrifuge tube: Assayed Multi-Sera Normal and Elevated.
Colorimetric Method For reproducibility control:-
Serum 0.5 ml Multi-Sera Low, Normal and Elevated.
Cat. No. Solution 5 1.0 ml
NOTES
TI 1010 100 ml 5. Iron Solution 1 x 100 ml Mix, let stand for 5-30 min at +20 to +25oC. Add one The supernatant must be absolutely clear after
6. Basic Magnesium spatula-full (180 mg) of basic magnesium carbonate from centrifugation. If not, the supernatant must be decanted and
Carbonate 2x 10 g bottle 6. Let stand for 30-60 min at +20 to +25oC, mixing centrifuged again. A swinging-head centrifuge should be
frequently during this period. Then centrifuge for 10 min at used and not an angle-head centrifuge.
PRINCIPLE(1,2) 3000 rpm. Remove supernatant.
An excess of iron is added to the serum to saturate the REFERENCE
transferrin. The unbound iron is precipitated with basic The supernatant can be stored for up to one hour. 1. Ramsey, W.N.M. The determination of the total
magnesium carbonate. After centrifugation the iron in the iron-binding capacity of serum. Adv. Clin. Chem. 1: 1,
supernatant is determined. Use 0.5 ml of supernatant for assay with serum iron kits 1958
(Cat. No SI 257 and Cat. No. SI 255) 2. Ferruccio Ceriotti and Giovanni Ceriotti, Improved
SAMPLE direct specific determination of serum iron and total
Serum. CALCULATION OF TOTAL IRON BINDING CAPACITY iron-binding capacity. Clin. Chem. 26: 327-331, 1980.
(TIBC)
REAGENTS
Using standard provided in Iron kit.
Contents Initial Concentrations of Solutions Asample
TIBC (mol/l) = x 107.4
5. Iron Solution Astandard
Iron 89.5 mol/l(500 g/100 ml)
6. Basic Magnesium Carbonate Asample
TIBC (mg/dl) = x 0.6
Astandard
PREPARATION OF SOLUTION
5. Iron Solution To calculate the unsaturated binding capacity (UIBC)
Ready for use. Stable until expiry date when stored subtract the serum iron concentration from the TIBC.
at +15 to +25oC.
6. Basic Magnesium Carbonate
Ready for use. Stable until expiry date when stored UIBC = TIBC - Serum iron concentration
at +15 to +25oC.

NORMAL VALUES (TIBC)

Serum: 46.4 - 69.5 mol/l (0.259 - 0.388 mg/dl)

Revised 25/04/96

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