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171 views2 pages

Blast Lab

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api-408976248
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© © All Rights Reserved
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Madison Gilbert

List the characteristics you observed about the unknown fossil.


Data-FOR EACH GENE:

Gene Organism that is Percentage What does Kingdom of Domain of the


most closely Identical? the gene the matched matched
matched? code for? organism? organism?
(Scientific and
Common Name)

1 Scientific​: Gallus 99% collagen animalia eukarya


gallus
Common​: chicken

2 Scientific​: 99% unknown animalia eukarya


Drosophila protein
melanogaster
Common​: fruit fly

4 Scientific​: alligator 100% unknown animalia eukarya


sinensis protein
Common​: chinese
alligator

Conclusion Questions: Please answer in complete sentences.


1.​ ​ Did the analysis of each gene support or refute your original hypothesis? Redraw the
cladogram from Part I or attach a picture. Show your hypothesis placement in green and
your actual placement (after data) in blue.
The results from my gene analysis supported my hypothesis that the fossil specimen belonged in
the same category as the crocodilians. The first and second genes were not a match to the fossil
specimen, while the fourth and last gene was a complete match, this confirmed my hypothesis.
These matches were based off the similarity of their amino acid sequences, which also enables us
to understand that their DNA shares similarities as well.
Madison Gilbert

2.​ ​ What other data could be collected from the fossil specimen to help properly identify
its evolutionary history?
​ e could further assess the specimen’s surroundings in order to analyze its environment, and any
W
adaptations that could have resulted from environmental changes. As well as what kinds of organisms
were found throughout the same vicinity as our fossil. For example, if multiple crocodile or alligator
fossils were found near our specimen, then we can infer that our fossil and those fossils share some kind
of relation. We could also detect any homologous or analogous structures in order to identify the
possibility of multiple common ancestors.

3. On the main page of BLAST, click on the link “List All Genomic Databases.” How
many genomes are currently available for making comparisons using BLAST? How does
this limitation impact the proper analysis of the gene data used in this lab?
There are currently 1,500 genomes available for analysis and comparison within the BLAST website.
This could hinder our gene analysis because, just as several organisms shared a 99% identical amino acid
sequence with our fossil, many organisms may share a 100% identical amino acid sequence with our
unidentified fossil. We are simply not obtaining all the possible genomes found throughout the world, so
the likelihood of us completely and specifically identifying our fossil is slim, but we can find a relatable
match fairly accurately.

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