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B.Pharm Microbiology & Biotech II

This document outlines the course contents for Pharmaceutical Microbiology & Biotechnology – II, which is a semester 5 subject for the B.Pharm program. The key topics covered include general microbiology concepts like microscopy, bacterial structure and classification. It also covers control of microbes in the pharmaceutical industry through disinfection and sterilization techniques. The document also briefly mentions DNA/RNA structure, genetic code, protein synthesis, and enzyme immobilization techniques. It concludes with listing the practical lab sessions and reference books for the course.

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266 views2 pages

B.Pharm Microbiology & Biotech II

This document outlines the course contents for Pharmaceutical Microbiology & Biotechnology – II, which is a semester 5 subject for the B.Pharm program. The key topics covered include general microbiology concepts like microscopy, bacterial structure and classification. It also covers control of microbes in the pharmaceutical industry through disinfection and sterilization techniques. The document also briefly mentions DNA/RNA structure, genetic code, protein synthesis, and enzyme immobilization techniques. It concludes with listing the practical lab sessions and reference books for the course.

Uploaded by

vanita
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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GUJARAT TECHNOLOGICAL UNIVERSITY

B.Pharm
SEMESTER: V
Subject Name: Pharmaceutical Microbiology & Biotechnology – II
Subject Code: 2250002

Teaching Scheme Evaluation Scheme


Theory Tutorial Practical Total Theory Practical
External Internal External Internal
3 0 3 6 80 20 80 20

Theory

Sr No Course Contents Total Hrs


1 Introduction and Scope of Microbiology 2
2 General microbiology: 19
a) Microscopy
 Light Microscopy (Bright Field, Dark field & Phase
contrast, Fluorescent, Differential interference )
 Electron Microscopy (SEM & TEM)
b) General Structure
 Prokaryotic and Eukaryotic Cell
c) Brief over view of 3 kingdom Classification system
d) Structure and Taxonomy of Actinomycetes, Bacteria,
Spirochetes, Rickettsia and Viruses, fungus with emphasis
on pathogenic and pharmacological importance.
e) Identification of microbes
 Staining Techniques
f) Nutritional requirements
 Nutrition requirements, Growth curve
 Introduction to various nutritional media,
 Cultivation and Isolation of bacteria, virus and fungus.
g) Bacterial count techniques
3 Control of microbes in pharmaceutical industry 14
a. Disinfection:
 Classification, mode of actions and Factor affecting
Disinfection
 Dynamics of Disinfection
 Evaluation of Disinfection
b. Sterilization:
 Introduction, significance, sensitivity of microorganisms,
 Detailed methods for sterilization processes.
 Sterilization control and sterility assurance.
4 Introduction of DNA & RNA, details of Genetic code and protein 06
synthesis
Introduction and scope of Biotechnology
5 Immobilization of Enzymes: 04
 Techniques of immobilization
 Factors affecting enzyme kinetics
 Applications

PRACTICAL – 22500P2

1. Introduction to Pharmaceutical Microbiology practical


2. Preparation of Various Media
3. Sub culturing of Common Bacteria (Aerobic and Anaerobic)
4. Staining of Microorganism (Monochrome staining, Gram staining, acid fast staining
etc)
5. Methods of Isolation
6. Study of Sterilization and Their Validation
7. Viable Counts and total counts by various methods
8. Evaluation of the disinfectants
9. Maintenance and preservation of pure culture

References Books:
1. Textbook of Microbiology by Tortora.
2. Pharmaceutical Microbiology, sixth edn, edited by W. B. Hugo and A. D.
Rusell Blackwell science.
3. Principles of Microbiology, Ronald M. Atlas. Second edn. W. C. Brown
Publishers.
4. Bergeys manual of Systematic Bacteriology, Williams and Wilkins- A
Waverly company.
5. Disinfection, Sterilization and Preservation. Fourth edn, Symour S. Black. Lea
and Febiger Philadelphia, London.
6. Industrial Microbiology. Fourth edn, Prescott and Dunn. CBS Publishers
and Distributors.
7. Principles of Fermentation Tehchnology. Second edn. P. F. Stanbury,
A.Whiteshaker and S. J. Hall Aditya Books Pvt Ltd. New Delhi.
8. Microbiology, Pelczar/Chan Kreig Tata McGraw Hill edn.
9. Industrial Microbiology L.E. Casida, Jr. New age International Publishers.
10. Fundamental Principles of Bacteriology. A. J. Sale, Tata McGraw Hill
Publishing Company Ltd.
11. Fundamentals of Microbiology by Forbischer.
12. Bentleys Text book of Pharamceutics.
13. Dispensing Pharmacy by Cooper and Gunn, Twelfth edn.
14. Remington Pharmaceutical Scicence, Latest edn.
15. Microbiology by Ronald Atlas.

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