XI-921 Series

Electrolyte Analyzer
User’s Manual

Caretium Medical Instruments Co., Limited

 Caretium Medical Instruments Co., Limited
Beishan Industrial Park 7th Floor Building 1, Beishan Road, Yantian,
Shenzhen 518083, China
Wellkang Ltd t/a Wellkang Tech Consulting
Suite B, 29 Harley Street, LONDON W1G 9QR, England, United Kingdom

Tel: 86 755 25273714 Fax : 86 755 25273096

Http://www.caretium.com [email protected]

Ver. 6.0
 REVISION LIST OF THE MANUAL
Rev./DATE REVISION DESCRIPTION
01/2013-04 First edition Ver. 6.0

Copyright
Copyright 2013, Caretium Medical Instruments Co., Limited, all rights reserved.

Thank you for choosing our instrument. The XI-921 series electrolyte analyzer
User’s Manual would bring you the best experience and conveniences.
This manual is compiled according to related laws and regulations of China and
the physical circumstances of XI-921 series electrolyte analyzer that
made.
Caretium Medical Instruments Co., Limited owns all the copyright of XI-921
series electrolyte analyzer sevice manual . Without expressly authorized by
Caretium Medical Instruments Co., Limited anybody or company can not duplicate,
copy , translate, or disclose this manual in any form.
This manual includes the newest information up to printing. reserve
the right of changing the content of this manual without prior notice.

1
 Contents
Chapter 1 General...................................................................................... 4
1.1 Scope of application .................................................................. 4
1.2 Instrument structure .................................................................. 4
1.3 Brief instruction of the instrument ......................................... 6
Chapter 2 Measuring principles ................................................................ 7
2.1 ISE theory..................................................................................... 7
2.2 Measuring principles ................................................................. 7
2.3 Manometric method (for HCO3-) .............................................. 8
Chapter 3 Features & Index .................................................................... 10
3.1 Measuring range & Electrode slope ..................................... 10
3.2 Technology index ..................................................................... 10
3.3 Features ........................................................................................ 10
Chapter 4 Installation ............................................................................... 12
4.1 Environment requirements ..................................................... 12
4.2 Unpack ........................................................................................ 12
4.3 Installation of new electrodes ................................................ 12
4.4 Installation of the tubes........................................................... 14
4.5 Installation of the reagents ..................................................... 15
4.6 Installation of the printing paper ........................................... 15
4.7 Installation of the auto sampler ............................................. 16
4.8 Connect to power supply ........................................................ 18
Chapter 5 Operation ................................................................................ 19
5.1 Flow Chart of the operating procedure ................................ 19
5.2 Startup and self-test ................................................................. 20
5.3 Sample measurement ............................................................. 23
5.4 Calibration.................................................................................. 30
5.5 W. List ......................................................................................... 32
5.6 Measure STD （Manual） ...................................................... 34
5.7 Service ........................................................................................ 37
5.8 Result review ............................................................................ 45
Chapter 6 Precautions ........................................................................... 47
6.1 Operation precautions............................................................. 47

2
 6.2 Safety precautions ................................................................... 47
6.3 Sample collection and handling ............................................ 48
Chapter 7 Maintenance .......................................................................... 49
7.1 Daily maintenance .................................................................... 49
7.2 Weekly maintenance ................................................................ 49
7.3 Spare parts replacement ......................................................... 49
7.4 Check the tubing system ........................................................ 49
7.5 Replace the electrode .............................................................. 50
7.6 Replace the reference membrane ........................................ 50
Chapter 8 Error code ............................................................................... 54
8.1 Printed error code ................................................................... 54
8.2 Displayed error code .............................................................. 54
Chapter 9 Troubleshooting ...................................................................... 55
9.1 Slope unstable ......................................................................... 55
9.2 Slope abnormal ........................................................................ 55
9.3 Aspiration abnormal ............................................................... 56
9.4 Cleaning and disinfection of autosampler tray .................. 56
Appendix 1：Instructions of De –protein solution ................................... 57
Appendix 2：User manual for filling solution (Drop bottle 15mL) .......... 57
Appendix 3：QC solution User’s manual ................................................. 58
Appendix 4：RS-232 standard output interface description .................. 59

3
 Chapter 1 General
1.1 Scope of application

This series electrolyte analyzers are automated, microprocessor controlled

analytic instruments that apply ISE (Ion Selective Electrode) technology to the
measurement of the contents of potassium (K), sodium (Na), chloride (Cl), calcium
(Ca), lithium (Li), pH and HCO3- （CO2）in human body liquid.
Instrument Models：
a) XI-921FT: K, Na, Cl
b) XI-921AT: K, Na,
c) XI-921BT: K, Na, Cl, & HCO3- (CO2)
d) XI-921CT: K, Na, Cl, Ca, pH
e) XI-921DT: K, Na, Cl, Ca, pH, CO2
f) XI-921ET: Li, K, Na, Cl
Note: XI-921□T Model, “T” –“Touch Screen” function

1.2 Instrument structure

a) XI-921FT、XI-921AT、XI-921CT、XI-921ET consists of mainframe, LCD

touch screen, printer, electrodes and reagent kits etc.

b) XI-921BT、XI-921DT consists of mainframe, LCD touch screen, printer,

4
 electrodes, electrochemical sensors and reagent kits etc.

c) XI-921AT、XI-921BT、XI-921CT、XI-921DT、XI-921ET、XI-921FT model back

picture.

5
1.3 Brief instruction of the instrument

Potassium, sodium, chloride, calcium and HCO3- make up the main composition of
body electrolytes. It is a prerequisite of all medical means to keep the balance of
human body electrolytes. And it is very important to obtain the amount of
potassium, sodium, chloride, calcium and HCO3- in patients’ body fluid.
In the past, flame luminosity method was widely used to measure the amount of
potassium and sodium. In recent years Ion Selective Electrode (ISE) technology
has developed with the application of sensor technology and micro-computer
technology. Flame luminosity method not only requires the flammable gas and the
compressed air, but also requires sample centrifuging to obtain the patients’
serum for dilution and test. While Ion Selective Electrode method can measure the
serum directly without any dilution, therefore it shortens the measuring time
significantly. In addition, Ion Selective Electrode method has several advantages:
faster, more accurate and less sample volume needed. It has become the
mainstream technology for electrolyte analysis.
These series electrolyte analyzers are specially designed for clinical analysis. The
main features include:
High precision: Guaranteed by long lifetime, high performance electrode
and advanced automatic control software. Unique calibration
programs eliminate systematic errors. Wide linear range.

Low sample volume: 100μl ~150μl per test only

High throughput: Result obtained in less than 60 seconds.

High automation: Automatic aspiration, washing and calibration. Results

display and print out automatically. （All models can add
auto sample plate, updated to auto sampler models.

Easy operation: User friendly software, large LCD display, touch screen. 24
hours non-stop working mode, suitable for emergency
samples.

Large memory: Up to 200 patient results stored on board, easy to review.

Easy maintenance: Advanced design of hardware, fluid tubing system and

self-diagnosis software, makes it easy and simple for
maintenance and troubleshooting.

6
 Chapter 2 Measuring principles
2.1 ISE theory

The analyzer utilizes Ion Selective Electrode (ISE) technology. Ion Selective
Electrode is a type of electrochemical sensor. It converts the ion activity to the
electric potential of the electrode. The relation conforms to the NERNST equation,
that the Logarithm of the ion activity has a linear relation with the electrode
potential. In addition, different electrode is sensitive to different ions, for example,
sodium electrode is only sensitive to Na ions, and potassium electrode is only
sensitive to K ions. If potassium electrode, sodium electrode, and chloride
electrode are being combined together, then K ions, Na ions, and chloride ions in
the sample can be measured at the same time.
The key part of the electrode is the sensitive membrane. On one side, it is in
contact with the sample, responds to the change of the concentration of certain
ions in the sample. On the other side, it is in contact with the internal filling
solution, and converts the ionic conduction to the electronic conduction through a
silver thread i.e. internal electrode. In addition, there is a reference electrode
providing reference potential and forming a complete measuring circuit. Inside the
reference electrode there is also an internal electrode. Its potential remains
constant when the concentration of the solution changes, so it provides a
reference point to measure the potential differences.

2.2 Measuring principles

The instrument measures the electrode potentials, and the data is processed by
the microprocessor to obtain the concentration of a given ion. The measure
method is called “standard comparison”. It uses two kinds of standard solutions,
one for the calibration of the base point, and the other for the calibration of the
slope. The result is obtained from the potentials of the sample and two standard
solutions.
Following are the equations:

CX = CA * EXP [(EX-EA) / S] （1）

EB - EA
S = ——————— （2）
Log（CB / CA）
Note:
Cx, Ex: the concentration and potential of the sample

7
 CA, EA: the concentration and potential of standard A
CB, EB: the concentration and potential of standard B
S: the slope of electrode
In order to improve the precision, the contents of the standard solutions should be
similar with the blood samples as much as possible.

2.3 Manometric method (for HCO3-)

Add certain quantity of blood serum and reagent (lactic acid) into the sealed
reaction chamber, the HCO3- ions in the serum will participate into the reaction and
release CO2, as a result, the gas pressure inside the reaction chamber will be
increased accordingly. The pressure sensor detects the changes and sends the
signals to the microprocessor to determine the amount of HCO3- ion of serum, and
then the amount could be displayed and printed. The instrument uses AB (Actual
bicarbonate) stand for HCO3- ion.

8
 K eyp ad

C om p u ter
A /D S creen

P rin ter

grou n d in g

K Na Ca PH Cl R ef

in let ou tlet

9
 Chapter 3 Features & Index
3.1 Measuring range & Electrode slope

Measuring range
Electrode Slope range (mV/dec)
(mmol/L)
K+ 0.50～15.0 27～70
+
Na 30.0～200.0 27～70
-
Cl 30.0～200.0 27～70
2+
Ca 0.10～5.00 15～35
pH 7.0～9.0(Unit) 27～70
+
Li 0.3～3.0 12～67
CO2 6.0～50.0 4～20

3.2 Technology index

Parameters Accuracy(B) Precision(CV) Linearity(D) Stability(S) Carryover(C)

K+ ≤3.0% ≤1.0% ≤3.0% or ±0.08 mmol/L ≤2.0% ≤1.5%

Na+ ≤3.0% ≤1.0% ≤3.0% or ±2.0 mmol/L ≤2.0% ≤1.5%

Cl- ≤3.0% ≤1.0% ≤3.0% or ±2.0 mmol/L ≤2.0% ≤1.5%

Ca2+ ≤5.0% ≤3.0% ≤3.0% or ±0.04 mmol/L ≤3.0% ≤1.5%

pH ≤1% ≤2.0% ≤5.0% ≤2.0% ≤1.5%

Li+ ≤5.0% ≤5.0% ≤5.0% or ±0.1mmol/L ≤5.0% ≤5.0%

CO2 ≤6.0% ≤3.0% ≤5.0% or ±1.0mmol/L ≤3 mmol/L ≤10%

3.3 Features

Principle: Direct measurement by Ion Selective Electrode (ISE)

Sample variety: Serum、Plasma（whole blood）
、Cerebrospinal fluid or Dilute urine
Sample Volume: 150 µl
Throughput: Up to 60 samples /hour
Data Storage: Up to 200 patient results
Calibration: Automatic or On-demand
Input: Touch-screen, barcode reader
Display: Large LCD with backlight
Output: Internal thermal recorder, RS-232 serial port
Ambient Conditions:
Temperature: 10℃- 30℃
Relative humidity: （20~85）%, non-condensing environment

10
Input Voltage: AC 210V ± 10%, 50/60Hz
Power consumption: 60VA
Dimensions: 380mm(L)×330mm(W)×410mm (H) (Main unit)
490mm(L)×400mm(W)×450mm(H) (With auto sampler)
Weight: Main unit – 8.0 kg
Auto Sampler (option) -- 1.5kg
Auto Sampler with plate –2.0 kg

11
 Chapter 4 Installation
4.1 Environment requirements

1) The instrument should be installed on a stable and solid platform that is

free of mechanical vibration and away from vibration source.
2) The environment should be as free as possible from dust, corrosive gas,
loud noises and electrical interference.
3) Avoid placing the instrument in direct sunlight or in front of a source of
heat or vent.
4) Ambient temperature: 10℃~30℃, relative humidity: (20～85) %.
5) The power supply should be AC210V±10%, 50/60Hz±1Hz
6) Power supply and grounding should be connected correctly.

4.2 Unpack

1）Check accessories refer to the packing list, contact supplier immediately if

there is any damage or missing parts.
2） heck instrument name and model code, please contact supplier if there is
any part not match.

4.3 Installation of new electrodes

1）Electrode assembly of new instrument has been installed and tested in good
condition, before shipping.
2）Check if the filling solutions are sufficient or carry bladders.

Replace & check electrodes

1) Assemble the electrodes with the rubber gasket according to figure.
2) Put them through the axis, and then tighten the nuts firmly.
3) If the filling solutions are insufficient, remove the internal electrodes and fill
in correct filling solutions (K filling solution for K electrode, Ca filling
solution for Ca electrode, Na/Cl filling solution for Na, Cl and pH electrodes,
and reference filling solution for reference electrode).
4) Clean and dry the electrodes with soft tissue.
5) Install the whole electrode assembly to the electrode holder.
6) Connect the electrode lead wires and the grounding wire to the
corresponding plugs according to figure 3.

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1) Do not mix up the electrodes!
2) Replaced internal electrode must not be used without distilled
water rinsing.
3) Away from metal goods
4) 80~90% solution is sufficient
5) Do not touch electrode membranes by any hard subjects.
6) Lapping electrodes to eliminate bladder
7) Electrode installation must be follow figure 2.
8) Do not mix up K、Ca、Na、PH、Cl solutions

Figure 2

13
 Figure 3

4.4 Installation of the tubes

Connect the tubes according to figure 4. Figure 5 is the tube connection

diagram for CO2 reaction chamber (for HCO3-)

Figure 4

14
 Figure 5

4.5 Installation of the reagents

1. For external reagent pack user: take off rubber cap of external reagents,
connect tubes according to outlet marks.
2. For internal reagent pack user: stretching the rubber cap, insert reagent
package.
3. XI-921BT、XI-921DT model user, should discharge waste water into the
external waste bottle (W).
4. Reagent bottle user: open each bottle cap（A std、B std、Lactic acid solution
）insert covered pipette into reagent bottle, screw down cap, and then connect
corresponding inhaling tube to the stainless steel tube.
5. Insert waste water tube to “W” waste bottle, screw down cap.
6. Finally, check out the whole tubing system.

After taking out standard、control、filling solutions or other reagents

from the refrigerator, please wait for a moment until they warm up to the
room temperature, to prevent the damage of the electrodes. Be careful not
to contaminate the reagents during the installation or replacing.

4.6 Installation of the printing paper

1. Open the printer cover, insert the paper support on the stand.
2. Insert the paper into the guide slot. Make sure the thermal side of the paper

15
faces downward.

Printer feeder
Printer paper
feed wheel

Printer
pressure lever

3. Pull up the lever on the right, rotate the knob until the paper comes out, then
push the lever down; or touch “Paper” on the service menu until the paper comes
out. As below figures.

4.7 Installation of the auto sampler

Auto sampler is an option, we will attach it with its accessory when

received customer order
1. Assembly the data line under bottom panel. Please follow the direction of below
figure to place two plugs of the data line.

Data line of auto

Bottom plate of
sampler
auto sampler

2. Remove the wash block cover. Then place main unit on the bottom panel and

16
make sure its four feet are fits in the holds of bottom panel. Then connect auto
sampler and machine by data line. (see below figure)

3. Put auto sampler on bottom panel, and make sure it is well connected by data
line. (see below figure)

4. The auto sampler will go clockwise and anticlockwise when switch on machine.
That self -detection will make sure the auto sampler is in good condition or not.
The locating point on auto sampler should be correspondent to the center of
wash block. Put sample tray on the auto sampler. (see below figure)

During self-testing, do not put sample tray on auto sampler, if the auto
sampler rotate anomaly, to prevent sample probe damage; Turn off the
power, remove auto sampler、 junction panel and data cable when problems
occur, then restart up the instrument, auto electrolyte analyzer turns into
semi-analyzer; please contact our distributor directly.

17
4.8 Connect to power supply

1. AC210V±10%，50/60Hz±1Hz (Unstable power users, need to add the regulated

power supply to make sure the input voltage is at correct level).
2. Make sure the power switch is at OFF position.
3.Connect the instrument to the power supply with the power cable.

The instrument must be grounded properly.

18
 Chapter 5 Operation
5.1 Flow Chart of the operating procedure

Start-up

Self test

Positioning

Calibration

Main menu

Sample Cal. W. list

STD Service Results

Sample Cal. W. list

Input work list

Measure sample Calibration

STD Service Results

Measure STD Result review

Time Position QC

Printer Cleaning Factor

Send Voltage Paper

Reagent Language

19
5.2 Startup and self-test

5.2.1 Startup
After the instrument being correctly installed, turn on the power and boot up the
instrument, the screen displays:
Initialization……
The instrument carries out the self-test for the positioner, distribution vavle, printer
and auto sampler. The sample probe will lift up, and the screen displays:

Auto position OK

Printer OK

Sample tray not detected

Notes:
1. For auto sampler models, it will show “Sample tray “OK” if the auto sampler
is correctly installed.
2. The initialization will halt if any error detected on the liquid distribution valve,
elevator switch or optical couplers.

5.2.2 ISE STD

When the initialization finishes successfully, the sample probe comes down, and a
few seconds later, the screen displays:
Mesure ISE STD…
1562 1587
0247 0247
2328 2306
86.00 77.92 76.75 50.01 55.79
66.63 82.10 70.68 40.82 79.98
84.99 77.01 77.58 49.64 57.86

It indicates the instrument is carrying out the calibration. The system checks the
positioner’ voltage, pump pulse numbers and electrode potentials. The screen
displays:
1562 … (the positioner’s voltage (in mV) when calibrating without liquid, up to 3
readings)
0247 … (the positioner’s voltage (in mV) when calibrating with liquid, up to 3

20
 readings)
2328 2306 … (the pump pulse number corresponding to the sample volume,
up to 4 readings)
86.00 77.92 76.75 50.01 55.79 (the potential of each electrode when Standard
B aspirated)
84.99 77.01 77.58 49.64 57.86 (the potential of each electrode when
Standard A aspirated)
……
…… (The potentials with standard B and standard A displayed in turn, up
to 3 times)
When the calibration finish, slope of electrodes will be show on the screen, as
below figure

Slope
K: 58.9 Pass
Na: 53.1 Pass
Cl: 49.4 Pass
Ca: 28.7 Pass
PH： 52.3 Pass

If the slope of an electrode is unstable, it will display “Fluc.”on the right side. As
below figure.

Slope
K: 50.5 Fluc.
Na: -0.2 Fluc.
Cl: 0.0 Fluc.
Ca: 0.3 Fluc.
PH： -0.6 Fluc.
OK
The normal ranges of the slopes are:
K: 27-70 mV/dec Ca: 15-35 mV/dec
Na: 27-70 mV/dec PH: 27-70 mV/dec
Cl: 20-70 mV/dec Li: 12-67 mV/dec
CO2: 4-20 mV/dec

5.2.3 Print STD result

After the calibration, and the results will also be printed out as below:

21
 Slope normal
range

If the slope of an electrode is abnormal, it will display “X” on the right side, as
below figure.

After the calibration, the screen will display the main menu,as below figure.

12-05-02
Main Menu 14:05:07

Sample Cal. W.List

STD Service Results

1. The test result is depends on STD results. The test result is considered

invalid if STD results are abnormal.

2. The STD result might be abnormal if the machine has not been used for
some time. In that case, User should operate STD at least 2 or 3 times to
make sure its electrode and mechanism are in good condition. Then the
correct STD results appeared. (with ok displayed on the screen)

5.2.4 Standby mode

If there is no operation for more than 20 minutes, the instrument will enter into

22
“standby” mode, the screen displays:

12-05-02
16:15:57

Wake up

For electrolyte analyzer with auto sampler (only) in the main menu, if there is no
operation for more than 20 minutes, the instrument will enter into “standby” mode.
Othervise, the instrument will not enter into “standby” mode.
Before machine enters into wake up mode, the probe will go down half and
after serval seconds, it will continue going down until it touches wash block. That
design is used to protect the probe in case any error operation damage it.
Meanwhile “Wake up” will be appeared on software.
In the standby mode, if the operator wants to measure sample again, just
press “Wake up” to active the instrument. If the standby time is less than 30
minutes, the instrument will be washing automatically when it waked up, then the
instrument will return to the working state before standby mode. If the standby time
is over 30 minutes, the instrument will do calibration automatically when it waked
up. The electrodes will be maintained during the standby time.

5.3 Sample measurement

5.3.1 Operations without auto sampler

Press Sample enter into sample measurement menu, the screen displays:

000000000
Sample ID000000000

001 Get ID Aspirate

Num:
U/L

Exit

Present the sample under the sample probe; press Aspirate , the sample will
be aspirated into the system. Remove the sample from the sample probe in time

23
when the screen shows “Remove sample” and beep sound gives out. The results
will be displayed within 55 seconds:

K: 4.96 pH: 7.64

Na: 147.8 AG: 31.0

Cl:109.0

Ca: 1.44
CO2: 7.8

And the results will also be printed out as below figure:

Samples of normal
reference value

Press Aspirate again，then continue to mesure the next sample.

Press Exit , the instrument will be back to main menu.
■ “H” indicates the result is higher than the normal range.
“L” indicates the result is lower than the normal range.
“?” indicates the slope is abnormal or the electrode has not been
calibrated properly. Please enter into STD to calibration again.
■ After press Aspirate , Remove the sample from the sample probe in time
when the screen shows “Remove sample” and beep sound gives out.
The screen displays:
000000000
Sample ID:
000000000
000000000
Num: 001 Get ID Aspirate

U/C

Exit

24
 If user failed to remove sample in time. The machine will give “beep”
warning.and the probe will not go down. In that case, user should click
Exit to make the probe down but the test result is considered as invalid.
The ERROR 3# will appear in test report. See as below figure:
Nếu người dùng không thể xóa mẫu kịp thời. Máy
sẽ cho "tiếng bíp"
warning.and thăm dò sẽ không đi xuống. Trong
trường hợp đó, người dùng nên nhấp vào
Thoát để thực hiện thăm dò nhưng kết quả thử
nghiệm được coi là không hợp lệ.
ERROR 3 # sẽ xuất hiện trong báo cáo thử
nghiệm. Xem như hình dưới đây:

Khi nhạc cụ phát ra When the instrument gives out beep sound, the operator should
tiếng bíp, người điều
khiển nên remove the sample from the sample probe immediately!
lấy mẫu ra khỏi đầu dò
mẫu ngay lập tức!
Once the system date changes, the sample number will restart from 001 and
increase until 255 (maximum) automatically. If the operator wants to change the
patient ID number munually, press the button Get ID, the screen displays:

ID:

1 2 3

4 5 6

7 8 9

./- 0 cls

Yes Exit

Then enter the patient ID number; press Yes to save.

If the operator wants to change the patient ID number by barcode reader, the just
scan the barcode on the sample tube. After being scanned, the ID will be
displayed after “ID”.

Operation method of normal serum sample and special sample:

In the sample measurement menu, press U/L , the screen displays

25
 Sample 000000000
ID:
000000000

Num: 001 Get ID Aspirate

Urine

Exit

Continue to press Urine, the screen displays

000000000
Sample ID:000000000
000000000
Num: 001 Get ID Aspirate

Lipemic

Exit

Continue to press Lipemic, the screen displays

000000000
Sample ID: 000000000

Num: 001 Get ID Aspirate

U/L

Đo mẫu nước tiểu: Nhấn U

/ L, khi khóa quay sang Exit
Nước tiểu, sau đó bắt đầu
đo lường. Trước khi đo
mẫu nước tiểu, hãy pha Normal sample measurement: Don’t press U/L, direct to press Aspirate , then
loãng mẫu nước tiểu với
Chất pha loãng nước tiểu aspirating sample to measuring sample (Sample don't need to pretreatment).
của caretium (Tỷ lệ pha
loãng: Nước tiểu: Chất
pha loãng nước tiểu = 1: Urine sample measurement: Press U/L, when the key turn to Urine, then start to
1).
Đo lưu lượng máu trong measuring. Before urine sample measurement, please dilute urine sample with
máu: Nhấn U / L, khi phím
quay sang Nước tiểu, tiếp Caretium’s urine diluent (Dilution ratio: Urine:Urine diluent = 1:1).
tục
để nhấn nước tiểu, chìa
khóa sẽ chuyển sang Lipemic blood measurement: Press U/L, when the key turn to Urine , continue
Lipemic, sau đó bắt đầu đo
(Mẫu to press Urine , the key will turn to Lipemic, then start to measuring (Sample
không cần tiền xử lý)
don't need to pretreatment).

26
5.3.2 Operations with auto sampler

User should assembly auto sampler according to step 4.6 and check
process is all right
The sample tray of auto sampler has 26 positions: 1～ 20 is sample
position; E ℃and E ℃are emergency sample position; QC℃and QC℃are control
position; the other is calibration and cleaning position. As below figure

QCⅡ QCⅠ EⅡ

Calibration
E Ⅰ

Cleaning

In the main menu, press“Sample”; enter into sample measurement menu, the
screen displays:

Sample

Tray 01 QC1
Num
First QC2
01

Last 20 Eme.1

Eme.2

Start Exit

Press the 01 key on the right side of the “Tray”, the screen displays:

Tray Num.
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

“Tray Num” is virtual number, as every sample tray has 20 sample positions, The
operator must be changed the tray number when the samples more than 20,

27
 otherwise it will be covered used sample number. Tray number can be from 01~12
input. Input the tray number, then press Yes to save .
Press the 01 key on the right side of the “First ”, the screen displays:

First Num.
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Input the first sample number (between 1 to 20), then press Yes to save .

Press the 01 key on the right side of the “Last ”, the screen displays:

Last Num.
2 3
1
4 5 6

7 8 9
Nhập số mẫu cuối cùng (giữa
số mẫu đầu tiên đến 20), sau . /_ 0 cls
đó nhấn Yes để lưu.
Để đo kiểm soát, nhấn QC 1 Yes Exit
và / hoặc QC 2, màn hình sẽ
hiển thị “QC
1 √ ”và / hoặc“ QC 2 √ ”. Sau Input the last sample number (between first sample number to 20), then press Yes
đó, trước hoặc sau khi thử to save .
nghiệm mẫu, thiết bị sẽ đo
các điều khiển được đặt ở vị
trí “QC 1” hoặc “QC 2” tự To measure the controls, press QC 1 and/or QC 2 , the screen will show “QC
động. Ví dụ, nếu nhà điều
hành chỉ nhấn QC 1, thì thiết 1 √” and/or “QC 2 √”. Then, before or after the test for the samples, the instrument
bị sẽ đo kiểm soát ở vị trí “QC will measure the controls placed on position “QC 1”or “QC 2”automatically.
1” trước khi thử nghiệm cho
tất cả các mẫu. Và nếu người For example, if the operator presses QC 1 only, then the instrument will
vận hành chỉ nhấn “QC 2”, thì
thiết bị sẽ đo kiểm soát ở vị trí measure the control on position “QC 1”before the tests for all samples. And if the
“QC 2” sau khi kiểm tra tất cả
các mẫu.
operator presses “QC 2” only, then the instrument will measure the control on
position “QC 2” after the tests for all samples.
Sample

Tray 01 QC1√
Num
First 01 QC2√

Last 20 Eme.1

Eme.2

Start Exit

28
 Trong quy trình đo
thường xuyên, nếu các
mẫu khẩn cấp đến, During the regular measuring procedure, if emergency samples come, the
nhà điều hành nên đặt
chúng vào vị trí “E 1” operator should place them on position “E 1” or “E 2”of the sample tray. If there is
hoặc “E 2” của khay mẫu.
only one emergency sample, then it must be placed on position “E 1”. Press “E
Nếu đó là
chỉ có một mẫu khẩn cấp, 1” or “E 2”, it will show “E 1√” or “E 2√”. As below figure.
sau đó nó phải được đặt
ở vị trí “E 1”. Nhấn “E Sample
1 ”hoặc“ E 2 ”, nó sẽ hiển
thị“ E 1√ ”hoặc“ E 2√ ”.
Như hình dưới đây Tray 01 QC1
Num
First 01 QC2

Last 20 Eme.1√

Eme.2√

Start Exit
Sau đó, sau khi đo mẫu hiện
tại, thiết bị sẽ chuyển sang đo
mẫu khẩn cấp. Khi đo các Then, after measuring the current sample, the instrument will turn to measure the
mẫu khẩn cấp kết thúc, “√” sẽ
biến mất khỏi màn hình, thiết emergency samples. When the measurement of the emergency samples finishes,
bị sẽ quay trở lại phép đo đối
“√” will disappear from the screen, the instrument will return to the measurement
với các mẫu thông thường
for the regular samples.
Trong quy trình đo mẫu,
nhà điều hành có thể nhấn
“Thoát” để thoát ra tại During the sample measuring procedure, the operator can press “Exit” to quit at
Bất cứ lúc nào. Sau đó, any time. Then, after finish the measurement for the current sample, the
sau khi hoàn thành phép
đo mẫu hiện tại, instrument will return to the main menu.
nhạc cụ sẽ trở lại menu
chính.
Trong trường hợp lấy mẫu In case the auto sampler fails, the operator can power off the instrument and
tự động không thành công,
người vận hành có thể tắt disconnect the auto sampler from the main unit. When power on the instrument
thiết bị và again, it will automatically switch to stand-alone working mode. Then the operator
ngắt kết nối bộ lấy mẫu tự
động khỏi thiết bị chính. Khi can continue to operate the instrument without the auto sampler.
bật thiết bị
một lần nữa, nó sẽ tự động
chuyển sang chế độ làm
việc độc lập. Sau đó, toán Operation method of normal serum sample and special sample:
tử
Enter the W.list menu , press Num. , the screen displays:
có thể tiếp tục vận hành
thiết bị mà không cần lấy
mẫu tự động Sample
Number
Num 01 Num

ID 00000000000000 Num

U/L

Exit

Then press U/L , the key will turn to Urine , continue to press Urine, the key will
turn to Lipemic , press it again will return to U/L.

29
 Normal sample measurement: Direct to press sample , enter into sample
measurement menu, then press Aspirate , start to measuring sample(Sample don't
need to pretreatment) .
Urine sample measurement: Press U/L, when the key turn to Urine, back to the
main menu. Then press Sample enter to measuring sample.
Before urine sample measurement, please dilute urine sample with Caretium’s
urine diluent (Urine:Urine diluent = 1:1).
Lipemic blood measurement: Press U/L, when the key turn to Urine , continue
to press Urine , the key will turn to Lipemic, back to main menu. Then press
Sample enter to measuring sample(Sample don't need to pretreatment) .

Note: 1. During testing process, Please click Exit in case any particular
Lưu ý: 1. Trong quá trình
kiểm tra, vui lòng nhấp vào condition occur. After clicking Exit machine will continue to finish
Thoát trong trường hợp cụ
the current test and then go back to main menu.
thể
điều kiện xảy ra. Sau khi 2. Formula for Sample number: (tray No.-1)X20+current tray no.
nhấp vào Thoát máy sẽ tiếp
tục kết thúc Therefore, user should fill up all 20 samples position before starting
kiểm tra hiện tại và sau đó
quay trở lại menu chính. the test. Otherwise the sampler number might be appeared empty in
2. Công thức cho số mẫu: the next tray.
(khay số-1) X20 + khay hiện
tại không. 3. In case any failure in auto sampler, User can shut down machine
Do đó, người dùng nên điền
vào tất cả 20 vị trí mẫu trước first, remove the auto sampler from machine. So that they can
khi bắt đầu continue their job by sampling manually.
các bài kiểm tra. Nếu không,
số mẫu có thể xuất hiện trống 4. Press sample to edit the sample number. Please refer to step
trong
khay tiếp theo. 5.5
3. Trong trường hợp bất kỳ
thất bại trong lấy mẫu tự
động, người dùng có thể tắt 5.4 Calibration
máy
đầu tiên, lấy mẫu tự động ra When systematic bias errors exist, the operator should calibrate the factors with
khỏi máy. Để họ có thể
tiếp tục công việc của họ control.
bằng cách lấy mẫu theo cách
thủ công. Present the control on the calibration position for auto sample tray.
4. Nhấn mẫu để chỉnh sửa số
In the main menu , press Cal. , the instrument enter into the calibration program,
mẫu. Vui lòng tham khảo
bước the screen displays:
5,5

Target

5. 00

145.00.

105.00

Cal. Exit

30
Press the button after K, the screen displays:

K:
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the target value of K and press Yes to save.

Enter Na and Cl target values in the same way. When all target values entered,
press Cal. , the screen displays:

Cal.

Aspirate

Exit

Present the control under sample probe, press Aspirate , the instrument aspirates
the control and tests it. Remove the sample from the sample probe in time when
the screen shows “Remove sample” and beep sound gives out. After 55 seconds
test finished, the screen displays:

K: 4.97 pH: 7.64

Cal.
Na: 146.8 AG: 30.7
Cl: 108.9

Ca: 1.44
CO2: 7.3
Exit

Press Exit to quit. Press Cal. to calibrate and obtain new factors, the
instrument will be calculated factors automatically, the screen displays the new
factors:

31
 K: 1.244

Na: 1.039

Cl: 1.042

And the new factors will also be printed out. As below figure.

5.5 W. List

5.5.1 Input manual

In the main menu, press W. List , enter into input number program, the screen
displays:

Sample Number

Num 001 Num

ID 000000000000000000 Num

U/L

Exit

The operator can press Num▲ or Num▼ to increase or decrease the

sample number. The operator can also change the number by press the Num
001 , the screen displays:

32
 Num
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the sample number and press Yes to save.

If the operator wants to change the patient ID number manually, and then press
the button ID000000000000000000 , the screen displays:

Num
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the ID number and press Yes to save.

5.5.2 Input by scanner

Please connect barcode reader to machine, see picture as follows

There are two ways to input data.

1. Scan by batch
Click “number” in software and then scan barcode on each tube by reader. The
patient ID will appear in software.
The sample number will skip to next automatically after scanning.
User can review the patient ID number by using the key Num ▼and Num ▲

33
 2. Single scan
User can scan one patience ID and do the test. After the test, they can start next
one.

5.6 Measure STD （Manual）

This program is to calibrate the slope of the electrode. The procedure is same as
that of “section 5.2 Startup and self-test”.

5.6.1 XI-921B & XI-921D mode

In the main menu, press STD , the screen displays:

Sel. ISE

Sel. CO2

Exit

Press Sel.ISE to calibrate the slope of K/Na/Cl/Ca electrode. The calibration

procedure is same as that of “section 5.2 Startup and self-test”.
To calibrate the pressure sensor, press Sel. CO2 , the screen displays:

CO2 STD

Aspirate

Exit

Present Standard AB under sample probe and press Aspirate to start the
calibration. The screen displays:

CO2 STD

Measuring…

Exit
34
After AB solution aspirated. Machine will calculate automatically and compare the
current result and previous result. If there is great difference between the two
results. Machine will start aspirating again. This process will be repeated up to 3
times. Finally if the result passed, it will be printed out. In fact, the STD results are
not reliable if machine demands aspiration in 3 times. In order to get reliable
results, user should do STD again and compare its current result and last result. If
the deviation range is within ±2, user can start to measure samples; othervise,
operator must be check the instrument refer to chapter 9 troubleshooting.

CO2 STD

CO2 9.7

Printing format as below:

For auto sampler models, the operator should place Standard AB on position
“QC1” of the sample tray.

1. After taking out the reagents from the refrigerator, and wait for a
moment until they warm up to the room temperature. This is to prevent
the possible damage of the electrodes.
2. Rock the reagents properly before test, for best calibration result.
3. Screw up cap; keep solution away from air contact.
4. For AB and TCO2 test: Blood must be completely sealed from air, during
sample taking, centrifuging and transporting process.

35
5.6.2 XI-921ET model

In the main menu, press STD , the screen displays:

Sel. A/B

Sel. C

Exit

Press Sel. A/B to calibrate the slope of K/Na/Cl electrode. The calibration
procedure is same as that of “section 5.2 Startup and self-test”.
And the results will also be printed out as below figure:

After calibration, the operator must be to calibrate LiK. Press Sel. C sample
probe lift up，the screen displays:

C STD

Aspirate

Exit

Then press Aspirate , aspirate C STD solution, normally, we need to STD C

less than three times, and then show as LiK 0.007.
Printing format show as below figure:

36
 The normal range of LiK is 0.006 to 0.012, (the ideal value is 0.008).

If new Lithiumk and the last Lik have obvious difference( the difference of two LiK
1. The Slop of Li là cơ sở về giá trị more than 0.002), the instrument will be automatically re-STD Lithium. Only with
STD của nó. Slop được coi là không
hợp lệ nếu correct LiK, can we obtain accurate Lithium slop.
người dùng không thể có được kết
quả chính xác của LiK.
2. Thông thường LiK khá ổn định và
người dùng phải làm STDＣ một lần
một tháng. 1. The Slop of Li is basis on its STD value. The slop is regarded as invalid if
3. Người sử dụng phải luôn luôn
nhận thức được slop của Na. Thông user can not get the correct result of LiK.
thường giá trị slop
phải lớn hơn 50. Người dùng có thể 2. Normally the LiK is quite stable and users are required to do STDＣ once
điều chỉnh giá trị slop bằng cách sử
dụng Na a month.
điều hòa. Nếu điện cực Na bị hỏng.
Họ nên dừng đo 3. USer has to always be aware of the slop of Na. Normally the slop value
Li cho phù hợp.
4. Người sử dụng nên làm STD C should be more than 50. User can adjust the slop value by using Na
lần nữa nếu họ thay thế điện cực mới
Li hoặc Na hoặc conditioner. If the Na electrode is broken. They should stop measuring of
điều chỉnh điện cực Na bằng Na điều
hòa. Li accordingly.
4. USer should do STD C again if they replace new electrode Li or Na or
adjust Na electrode by Na conditioner.

5.7 Service

The instrument has comprehensive service programs. Press Service in the

main menu, the screen displays:

Service

Time Position QC

Printer Cleaning Factor

Send Voltage Paper

Reagent Language

Exit

37
5.7.1 Change time

Press Time , the screen displays:

Year:

1 2 3

4 5 6

7 8 9

./- 0 Cls

Yes Exit

Enter the value of “Year”. Press Yes to save. Set the month, date and time in
the same way.

5.7.2 Position

If the positioner is defective, operator can be check by manually.

Pull out liquid positioner and re-connect the tubing. This program is to setup the
correct aspiration volume. Press Position in the Service menu, the instrument
will be automatically aspirated Stnadard A solution, the screen displays:

Position

Repeat

Please Position

Exit

The operator should check if the liquid reaches the correct position: the liquid
surface should be about 2cm away from the inlet of the electrode assembly. Show
as below figure:

38
Press ▲ to increase the aspirating volume if the liquid does not reach that
position, press ▼ to decrease the aspirating volume if the liquid exceeds the
position. It is suggested to run this position program after any services performed.
The above operation can be skipped if the instrument has a positioner (fluid
position sensor) installed.
However, if the positioner is defective, the instrument will use the parameter that
obtained from the above operations. For this reason, it is always suggested to run
this program when installing a new instrument.

5.7.3 Quality Control

To run QC program, press QC in the Service menu, the screen displays:

QC

Aspirate

Stat.

Lot No.

Exit

To enter a new Lot number, press Lot No. , the screen displays:
Lot No.
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the new lot number (8 digitals, such as 20050330); press “Yes” to save.

39
 Note: All QC data stored in the instrument will be deleted automatically
when a lot number entered!

Present control under sample probe, press Aspirate , the control will be
aspirated into the system for measuring. (For auto sampler models, the control
should be placed to“QC1” position of the sample tray.)
The result will be displayed and printed out within 60 seconds.
When there exist 5 QC results or more (up to 255), a statistical report can be
obtained. Press Stat. , the screen displays:
Lưu ý: Tất cả dữ liệu QC được lưu trữ trong công cụ sẽ bị
xóa tự động
khi nhập số lô!
Kiểm soát hiện tại dưới đầu dò mẫu, nhấn Aspirate, điều
khiển sẽ By Day
hút vào hệ thống đo. (Đối với các mẫu lấy mẫu tự động,
điều khiển
nên được đặt ở vị trí “QC1” của khay mẫu.) By Month
Kết quả sẽ được hiển thị và in ra trong vòng 60 giây.
Khi có 5 kết quả QC trở lên (tối đa 255), báo cáo thống kê
có thể là
thu được. Nhấn Stat. , màn hình hiển thị:

Press By Day , then the screen will show a statistical report including mean
value (Mean), standard deviation (SD), coefficient variation (CV %).

MEAN SD CV

K：4.95 0.02 0.41

Na：144.5 1.97 1.36

Cl：104.5 0.13 0.12

Ca： 1.44 0.01 0.35

CO2： 7.84 0.24 3.06 Exit

The report will also be printed out as below:

If press By Month ; it will print out a statistical report for all the last QC results
of each day.

5.7.4 Printer

40
Press Printer in the Service menu, the screen displays:

Print ON

Print OFF

Press Print ON to enable the printer; press Print OFF to disable the printer.

5.7.5 Clean the electrode

Press Cleaning in the Service menu, the screen displays:

Cleaning

Deprotein.

Na Adjust

Exit

5.7.5.1 Deprotein
For auto sampler models, the operator should place the cleaning solution to the
“Calib” position of the sample tray.
Press Deprotein , Aspirate shows on the screen.

Cleaning

Aspirate

Deprotein

Na Adjust

Exit

Present the cleaning solution under sample probe. Press Aspirate to aspirate
the cleaning solution into the instrument. After aspiration, Aspirate disappears and
the percentage of the progress appears on the screen:

41
 Cleaning

Deprotein

Na Adjust
015%

Exit
The whole process will take about 5 minutes.
If any special reasons to suspend the program, press Exit directly to exit the
program. Trình bày điều hòa Na dưới đầu dò mẫu. Nhấn Aspirate để hút
Na điều hòa vào dụng cụ. Sau khi khát vọng, Aspirate biến mất và
Present the cleaning solution
tỷ lệ phần ontrình
trăm của tiến thexuất
“calibration” position of sample tray with auto
hiện trên màn hình.
Mất khoảng 1 phút để hoàn thành chương trình Na Adjust.
sampler. Nếu bất kỳ lý do đặc biệt nào để tạm ngưng chương trình, hãy nhấn Thoát trực tiếp để thoát khỏi
chương trình.

5.7.5.2 Na adjust
Press Na Adjust , the screen displays:

Cleaning
Trình bày điều hòa Na dưới đầu dò mẫu. Nhấn Aspirate để
hút Na điều hòa vào dụng cụ. Sau khi khát vọng, Aspirate
biến mất và tỷ lệ phần trăm của tiến trình xuất hiện trên màn Aspirate
hình.
Mất khoảng 1 phút để hoàn thành chương trình Na Adjust.
Nếu bất kỳ lý do đặc biệt nào để tạm ngưng chương trình,
Deprotein
hãy nhấn Thoát trực tiếp để thoát khỏi
chương trình. Na Adjust

Exit

Present the Na conditioner under sample probe. Press Aspirate to aspirate the
Na conditioner into the instrument. After aspiration, Aspirate disappears and the
percentage of the progress appears on the screen.
It takes about 1 minute to finish the Na Adjust program.
If any special reasons to suspend the program, press Exit directly to exit the
program.
Note:
If Na slope more than 45, ignore “Na adjust” program

5.7.6 Change factor

To change the calibration factors manually, press Factor , the screen displays:

42
 a b
Exit K: x1.000+.000

Na: x1.000+.000
K:
Cl: x1.000+.000
Ca:: x1.000+ 000

Note: a – slope, b – intercept

Select the item to be changed, for example K, then the screen displays:

K: a
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the new slope value (a) and press Yes to save, then the screen displays:

K: b
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Enter the new intercept value (b). Press Yes to save.

5.7.7 Data transfer

Press Send , the patient results will be transferred to the host computer.

5.7.8 Voltage

Press Voltage to check the potential of each electrode:

43
 Voltage

Aspirate.

Exit

Present the QC solution under the sample probe, then press Aspirate to check
the voltages. The screen displays the voltages (in mV) every two seconds:

Voltage

K: 67.36 CO2: 2.08

Na: 79.46

Cl: 70.61

Ca: 41.36
PH: 76.82 Phot.: 1601 Exit

For auto sampler models, the operator should place the QC solution to the “Calib”
position of the auto sample tray.
Press Exit to quit.
The operator can check the performance of the electrodes by this program. For
standard A or control, the normal values of K, Na and Cl should be about 50.
If the values of K, Na and Cl are all smaller than 20, then most likely the reference
filling solution or the reference membrane is getting aged and should be replaced.

5.7.9 Feed paper

Press Paper to feed the printing paper.

5.7.10 Reagent
To check the residual volume of the reagents, press Reagent , the screen
displays:

Std A: 650 ml
StdB: 280 ml
Date:12-05-03 16:00
No.: 000000

Refill Exit 44
The residual volume of the reagents will show on the screen. When replace a new
reagent pack, the operator should press“Refill”to reset the values.
Note:

1. When icon appears on the main menu screen, it indicates the

remaining reagents can run about 150 samples.

2. When icon appears on the main menu screen, it indicates the

remaining reagents can run about 20 samples only.

3. When icon appears on the main menu screen, it indicates the reagents
are run out.

5.7.11 Language
Press Language , the screen displays:

English Spanish French

Italian

Exit

This instrument has four languages to choose, English, Spanish, French, Italian.
The operator can according to your need to change the language.

5.8 Result review

The instrument only stores one-day patient results for review.

Press Results , the screen displays:
000000000
Results ID: 000000000

K: 5.03 PH 7.56 Num001

Na: 145.5 AG 31.0

Cl: 105.2

Ca: 1.30 Day 22 Print

CO2 7.8 All Exit

45
To review the result by sample number, press Num 001 , the screen displays:

Num
2 3
1
4 5 6

7 8 9

. /_ 0 cls

Yes Exit

Input the sample number, press Yes , then the corresponding result will be
displayed on the screen. The operator can also press “▲” or “▼” to change the
sample number.
Press Print to print out the sample result.
Press All to print out all sample results. If there is no data stored, the
screen will display “No data”.
Note: to print out the results, the printer should be enabled first. Refer to section
4.6
The printing format is as following:

Printing format of single sample review Printing format of all samples review

46
 Chapter 6 Precautions
6.1 Operation precautions

1. The analyzer is designed to work continuously for 24 hours a day. No

need to shut down the machine everyday.
2. Do not use the standard solutions for flame luminosity. They include strong
acid and other supplements that may damage the electrodes.
3. Not all commercial controls are suitable for ISE measurement. Some of
them contain too much chemical additives that may interfere in the
measurement.
4. The bubbles should be excluded during the sample aspiration; otherwise
the results will be unreliable.
5. When the sample reaches the checking point, make sure there are no
bubbles inside; otherwise the sample should be measured again.
6. Don’t use original tube for sampling directly after centrifugation. That will
cause clog easily. We suggest to remove serum to small sample cup by
pipette, and then put the cup on auto sampler.
7. If the ambient temperature fluctuates for more than 10degree, the
instrument should be calibrated again.
8. The pH value of the standard solutions and the samples should be within
6-9, otherwise it will interfere in the measurement of sodium ions.
9. Discard the reagent if mildew or deposition found.
10. Perform the routine maintenance according to the instructions.
11. Every electrode provided Lot number on its surface. User has obligation
to show lot number when require service. Otherwise we can not offer any
service for repair or replacement.

6.2 Safety precautions

1. The electric voltage inside the instrument may be harmful to human body.
Do not open the instrument before disconnect it from the power supply.
2. Because the samples may have pathogenesis bacterium or viruses, all
replaced tubes, electrodes and waste containers should be discarded
according to the safe laboratory procedures and government regulations.
3. The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.
4. The reagents are harmful to human body. If the reagents accidentally spill
on your skin, wash them off with plenty of water and if necessary, go to

47
 see a doctor.
If the reagents accidentally spill into your eyes, wash them off with plenty of water
and immediately go to see a doctor

6.3 Sample collection and handling

Sample collection and handling must be carried out by the professionals. Always
avoid the hematolysis. In addition, the following points should be noted:

1. The serum or plasma can be stored in the refrigerator, but they must be
warmed up to the room temperature before test.
2. When preparing the blood serum samples, do not add any materials like the
surface active agent that may interfere in the measurement or even damage
the sensor.

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 Chapter 7 Maintenance
7.1 Daily maintenance

Pay attention to the reagent residual volume; replace the reagent pack if
necessary.

7.2 Weekly maintenance

1. Check each electrode; see as if the internal filling solution is sufficient.

Refill the filling solution if it is less than 2/3 of the total volume.
2. Check if there is salty crystal on the electrode, if so, clean it with wet
tampon.
3. Check if the sample aspiration volume is correct (sample reaches the
checking point), if necessary, adjust the pump according to section 4.7.2.
4. Run cleaning program in service menu once a week if more than 20
samples measured every day. If less than 10 samples measured every
day, then the user just need to run the cleaning program every 2-3 weeks.
Please refer to section 4.7.5.
5. Run “Na adjust” program if the slope of Na electrode is less than 45.
Please refer to section 4.7.5.
6. Check the voltage of each electrode. If necessary, replace the reference
filling solution or reference membrane. Please refer to section 4.7.8.

7.3 Spare parts replacement

Check the following parts periodically:

1. Pump tube
2. Aspirating tube
3. Connecting tubes
4. Valve
5. Internal electrodes
If necessary, replace the aged part.

7.4 Check the tubing system

If the aspirating speed and volume is abnormal, check the tubing system to
see if there is any leakage.
1. Run the calibration program.
2. Check the flow inside the electrode assembly.
3. Check the flow inside the tubing system.
4. The normal condition is a long section of air followed by a long section of

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 liquid.
5. If the tubing connection loose, then bubbles can be found near the connector.
Connect the tubing again.
6. If somewhere between the electrodes leaks, then disassemble the electrodes
and check the gasket.
7. If the tubes are being tightly connected, then check if there is any blockage.
8. Replace the pump tube if it is seriously distorted, otherwise the aspiration
volume will not be sufficient.

7.5 Replace the electrode

1. Pull out all electrode wires from the plugs. Remove the tubes from the inlet
and outlet of the electrode assembly.
2. Loose the fixing nuts of the electrode assembly.
3. Disassemble the whole electrode assembly. Discard the electrode to be
replaced.Take a new electrode and add the filling solution.
4. Assemble the electrodes in correct order. Make sure that the rubber gaskets
are in right position.
5. Tighten the fixing nuts of the electrode assembly.
6. Connect the tubing. Insert the electrode wires back to the plugs.
7. Calibrating the instrument.

7.6 Replace the reference membrane

See following figures:

1 2 3 4

5 6 7
1. Ref 2. Screw cap 3. Reference membrane 4. internal electrode

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5. Internal interface 6. Fitting tool 7. “O” ring

1）Open the front door, then pull out the lead wires of electrodes from the plugs.
Remove the tubes from the incoming and outcoming of the electrode assembly.
See the following figure:

2）Loosen the fixing nuts of the electrode assembly and take out the reference
electrode. See the following figure:

3）Loosen the screw cap, take out the internal electrode, and remove the filling
solution, O-ring and old reference membrane. Clean the internal interface and Ref
with distilled water, and wipe it with clean cotton or tissue.
4）Place a new reference membrane in the distilled water to get it softened.
5）Place the fixed reference membrane into fitting tool with ‘O’ ring. See the
following figure:

6) Convert the internal interface and put the reference membrane on the top of it.
See the following figure:

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7）Connect the fitting tool with the internal interface and pull the ‘O’ ring onto the
internal interface. See the following figure:

8）Remove the interface. Make sure that the reference membrane is smooth and
not damaged. See the following figure:

9）Put the internal interface into Ref vertically, and then tighten the screw cap. See
the following figure:

10）Fill the internal interface with reference solution with an syringe (up to 2/3 of
the total volume excluding the bubbles in the bottom)
11）Screw on the reference internal electrode. If there is any filling solution
overflowed, wipe it with clean tissue. Otherwise, it will produce white salt
crystals after evaporation of water and might affect test results.
12）Re-assemble the electrodes in correct order, tighten the fixing nuts, and then

52
 re-install the incoming and out-coming tubing. Insert all the wires into the
plugs. Run calibration again.
Notes: The instrument may give “Error” signals in the first calibration after
replacement of the membrane. In this case, perform calibration again and the
results will be good. If the results are still not good after several calibrations, check
as following:
●Check whether the reference membrane is properly replaced. Replace it if
necessary.
● Check whether the reference filling solution is invalid or contaminated.
Replace new reference filling solution if necessary.
● Check whether there is bubble at the bottom of the reference electrode.
Remove the bubble.
● Check the internal electrode and make sure its coating doesn’t flake off.
Change it if necessary.

Note：
★ Order of replace the membrane: place the membrane→ place the internal
interface onto the Ref→ tighten screw cap→ inject Ref filling solution→
tighten internal electrode
★ Take a piece of new membrane with a pair of tweezers, soften it with
distilled water for some seconds, then tighten internal electrode. Clean
the Ref electrode with clean tissue.
★ Look at below figure. At the area of the hole located between the
membrane and the liquid flow, some deposit of protein can be found; its
color is dark red, easily identified. During the replacement of the
reference membrane procedure, check and clean the sample passing
hole with a pinhead firstly. Make sure NOT TO damage the electrode
channel.

Blocked hole

53
 Chapter 8 Error code
8.1 Printed error code

Code Description
Error 0# Liquid positioning failed
Error 1# Liquid detecting failed
Warn 2# Bubbles detected
Error 3# Too much or less sample aspirated
Error 4# Slope abnormal
Error 5# Slope unstable

8.2 Displayed error code

Code Description
1# Liquid distribution valve failed
2# Elevator switch failed
3# Optical coupler for the sample tray origin failed
4# Optical coupler for the sample tray cup failed

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 Chapter 9 Troubleshooting

9.1 Slope unstable

Cause Recommended Solution

Unreliable grounding Check the connection of the grounding
wire

No standard A or standard B aspirated Check and replace Standard A or B;

Check the tubing connection
Incorrect positioning Adjust positioning again
The reference filling solution or Replace the reference filling solution or
reference membrane not working reference membrane when necessary
The internal electrode turns gray Replace it when necessary
Poor connection of the electrode wire Check and connect again
Electrode membrane leaks Replace it when necessary
Power supply voltage fluctuates Use UPS or power stabilizer
Humidity too high Lower the humidity or move the
instrument to a dry place
Bubbles inside the tubing Check the tubing system
Liquid leakage inside the valve Replace gasket or valve when necessary
The electrode is not activated or the Activate the electrode first
activating time is insufficient

9.2 Slope abnormal

Cause Recommended Solution

Too many organic deposits on the Wash it with de-protein solution
electrode membrane
Reagent contaminated Replace the reagent
Insufficient filling solution Refill the filling solution
Electrode does not work Replace the failed electrode
Dust or moisture around the Clean and dry the electrode and plug
electrode or plug

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9.3 Aspiration abnormal

Cause Recommended Solution

Aspirating tube loose or broken Connect again or replace it
Pump tube sticks Restore the tube
Pump tube broken Replace the tube
Pump tube blocked Clear the blockage
The gasket between the electrodes Place the gasket properly
does not placed properly or missing
The electrode assembly leaks Tighten the assembly again
Dust on the positioner or Clean or replace
positioner damaged
Print “NO STDA” Check the Standard A solution
Check if there is leakage or blockage
in the tubing system
Print” AIR IN SENSOR” Check the Standard A solution
Check if there is aspirate sample or bubble
in the tubing system

9.4 Cleaning and disinfection of autosampler tray

Please check the autosampler tray to ensure whether it is polluted or not. If it is the
case, please use disinfectant to clean it.

Method:
From the instrument remove the transparent tray of autosampler tray, add 2% "84
Disinfectant" (or 2% glutaraldehyde solution and other disinfectant) and soak for 30
minutes, then wash it with clean water, and wipe it clean; you may use the same way
to clean the cover of the autosampler.

56
Appendix 1：Instructions of De –protein solution
There are two kinds of cleaning solution, daily cleaning solution(blue) and weekly
cleaning solution(coloress).The cleaning solution with alkalescence can be use for
removing fibers and protein, which remain in tube system after tests. The daily
cleaning solution only for the machine with auto sampler.
After De -protein, user should operate calibration in several times until slops
become stable.
The cleaning solution should be placed in a cool dark place.

Appendix 2：User manual for filling solution (Drop bottle 15mL)

The filling solution inside each electrode would be decreased when being used in
a certain time. If the liquid level lower than 2/3 of the chamber that would be effect the
test results. To avoid any error, please add filling solution in the proper time.

Make sure to add filling solution into the corresponding electrode. ( K filing
solution for Potasium; Na,Cl,Ph filling solution for Sodium, Chloride and Ph electrode;
Ca filling solution for Calcium; Li filling solution for Lithium and REF filling solution for
reference electrode).
First of all, Open the lid of bottle by turning in counterclockwise. Put the head of
bottle into the elctrode and then fill the electrode up to 2/3 of the chamber.
After filling, seal the lip by turning in clockwise and store it in a cool dry place
away from light.

1、Keep the gap between the head of bottle and chamber when solution filling.
Otherwise, It would be difficult to add solution. (overflow or no solution out)
2、Try to use tissue to dry the residual liquid when it is founded in the black plastic
portion of electrode. Otherwise, it is difficult to to add solution. (overflow or no
solution out)
3、In case the electrode go bad in cold weather during transportation, User would
get the empty electrode without filling solution inside. Please follow the steps
above to fill the electrode before using.

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Appendix 3：QC solution User’s manual
Caretium offers four kinds of QC solution:
QC Solution (K, Na, Cl)
QC Solution (K, Na, Cl, Ca)
QC Solution (K, Na, Cl, Li )Low value
QC Solution (K, Na, Cl, Li)High value
The QC solutions are only for evaluating performance of machine (All test
results should be within the measuring range). However, the results by QC solution
should not be used judge accuracy and user can not use it for calibration.

58
 Appendix 4：RS-232 standard output interface description
(1) Features
Electrical specification：accord with EIA RS-232C
Stop bit：1
Data bits：8
Parity check bit：No
Transmission speed： 19200 baud
(2) Data format
001 000000000000000000 063 5.23 151.2 111.4 1.44 7.62
Note：Serial number+ Space +ID+ Space + Flag byte + Space +K+ result +Space+
Na+ result + Space + Cl- result + Space + Ca2+ result + Space + PH result+ Space.

(3) Flag byte instructions：

Digit 7 Digit 6 Digit 4 Digit 3 Digit 2 Digit 1 Digit 0
1: Aspiration 1： Li+ result 1：pH STD 1：Ca2+ STD 1：Cl- STD 1：Na+ STD 1：K+ STD
abnormal 0： Cl- result Not Pass Not Pass Not Pass Not Pass Not Pass
0：Aspiration 0：Ph STD 0：Ca2+ STD 0：Cl- STD 0：Na+ STD 0：K+ STD
normal Pass Pass Pass Pass Pass

(4)RS-232 interface connection

XI-921 electrolyte analyzer PC

2 2
3 3
5 5

(5) Result transmission and format

Machine can print out test results and transit them in real time to computer by
RS-232 port. The result which transited to computer is same as printed one. If
results are not transited in time. User can click the key “Send data” to send them
manually.

59