Avian Pathology
Bacterial diseases: histo slides
ECVP-ESVP Summer School 2012
Frédérique NGUYEN
Bacterial diseases: histo slides
B1. Turkey.
Organs? Morphologic diagnosis? Special procedure?
B2. Hen.
Organ? Morphologic diagnosis? Etiologic diagnosis?
B3. Chicken.
Organ? Morphologic diagnosis? Etiology?
B4. Pigeon.
Organs? Morphologic diagnosis? Special procedure?
011381A
B1. Turkey (2 organs)
Organs? Keywords?
Morphologic diagnosis?
Special procedure needed to confirm your etiologic diagnosis?
011381A
B1. Turkey
Liver.
Diffuse lesion.
011381A
B1. Turkey
Sinusoids: hyperemia.
Kupffer cells: hypertrophy.
011381A
B1. Turkey
Sinusoids: hyaline thrombosis (fibrin)
Disseminated intravascular clotting
Lymphocytes
011381A
B1. Turkey
Sinusoids: extracellular, intravascular basophilic 1-micrometer anucleated
microorganisms (bacteria) + intracytoplasmic in Kupffer cells.
Hypercellularity (leukostasis)
011381A
B1. Turkey
Gram stain
Colonies of Gram-positive cocci (1 or 2 micrometers in diameter) within
the sinusoids and the cytoplasm of Kupffer cells.
011381A
B1. Turkey
Liver, acute diffuse moderate hepatitis with sinusoid hyperemia,
thrombosis and leukostasis, and intravascular presence of bacterial
colonies: septicemia
011381A
B1. Turkey
Spleen.
Multifocal lesion.
011381A
B1. Turkey
Acute vasculitis: thrombosis, medial necrosis.
011381A
B1. Turkey
Fibrinoid necrosis of both the white and red pulp.
Micro-hemorrhages (extravasated red blood cells).
Lymphocytic pyknosis, karyorrhexis.
011381A
B1. Turkey
Sinusoids: extracellular, intravascular basophilic 1-micrometer anucleated
microorganisms (bacteria) + intracytoplasmic in macrophages.
Red pulp: macrophage hypertrophy (activation, phagocytosis).
011381A
B1. Turkey
Gram stain
Intravascular, extracellular colonies of Gram-positive cocci
011381A
B1. Turkey
Spleen, multifocal acute marked necrotizing splenitis with intralesional
extracellular intravascular bacteria and septic vasculitis, septicemia.
Bacterial diseases: histo slides
B1. Turkey.
Organs? Morphologic diagnosis? Special procedure?
B2. Hen.
Organ? Morphologic diagnosis? Etiologic diagnosis?
B3. Chicken.
Organ? Morphologic diagnosis? Etiology?
B4. Pigeon.
Organs? Morphologic diagnosis? Special procedure?
890989
B2. Hen
Organ? Keywords?
Morphologic diagnosis?
Etiologic diagnosis?
890989
B2. Hen
Liver
The architecture of the liver is markedly distorted by random, multifocal to
coalescing, up to 1-mm diameter, variably encapsulated, granulomas.
890989
B2. Hen
The larger granulomas contain, from the centre to the periphery: a central mass
of amorphous hypereosinophilic material with karyorrhectic and pyknotic
nuclear debris (caseous necrosis); cholesterol clefts;
890989
B2. Hen
… surrounded by a thick rim of large, pale acidophilic cells with indistinct
borders and round hypochromatic nucleus (epithelioid macrophages);
giant multinucleate cells with abundant eosinophilic cytoplasm and either
peripheral nuclei (Langhans-type giant cells) or haphazardly-placed
nuclei (foreign-body-type giant cells);
890989
B2. Hen
… moderate amount of lymphocytes, few plasma cells and heterophils;
a thin rim of fibrosis. Within granulomas, some macrophages are round,
large, and filled with an empty cytoplasmic vacuole (lipid-laden
macrophages).
890989
B2. Hen
Smaller granulomas contain epithelioid macrophages, Langhans giant
cells, foreign body giant cells, and do not possess a necrotic centre.
890989
B2. Hen
Fite-Farraco stain
Acid-fast bacilli within the necrotic centres of the granulomas, low amount
890989
B2. Hen
Within the remnant hepatic parenchyma, there are multiple foci of
extramedullary heterophilic granulopoiesis (especially periportal)
and lymphopoiesis ± amyloid deposition.
890989
B2. Hen
Multifocal to coalescing marked granulomatous hepatitis and
perihepatitis with caseous necrosis.
Mycobacterial hepatitis and perihepatitis.
Bacterial diseases: histo slides
B1. Turkey.
Organs? Morphologic diagnosis? Special procedure?
B2. Hen.
Organ? Morphologic diagnosis? Etiologic diagnosis?
B3. Chicken.
Organ? Morphologic diagnosis? Etiology?
B4. Pigeon.
Organs? Morphologic diagnosis? Special procedure?
021308
B3. Chicken
Organ? Keywords?
Morphologic diagnosis?
Etiology?
021308
B3. Chicken
Brainstem (and cerebrum) (and cerebellum)
021308
B3. Chicken
In the brainstem (and cerebrum, not in all slides) is (are) one (two) well-
demarcated, unencapsulated, 1-3 mm of diameter, necrotic focus
(foci), in which …
021308
B3. Chicken
the neuropil is distended by coalescing empty spaces (edema),
neurons and glial cells are shrunken, hyperchromatic with pyknotic nucleus
(necrosis)
021308
B3. Chicken
capillary blood vessels are occluded by an amorphous eosinophilic
material (fibrin) and red blood cells (hyaline thrombosis)
few red blood cells are extravasated (mild hemorrhage).
021308
B3. Chicken
neurones are devoid of Nissl bodies (chromatolysis),
there are microglial cells and lymphocytes within Virchow-Robin spaces of
cerebral blood vessels (perivascular cuffing),
there is rarefaction of myelin sheaths (demyelination) and multifocal
axonal dilatation (spheroids).
021308
B3. Chicken
Focal (bifocal) acute cerebral necrosis with edema,
demyelination and capillary hyaline thrombi.
Enterococcus hirae, or Enterococcus durans
Bacterial diseases: histo slides
B1. Turkey.
Organs? Morphologic diagnosis? Special procedure?
B2. Hen.
Organ? Morphologic diagnosis? Etiologic diagnosis?
B3. Chicken.
Organ? Morphologic diagnosis? Etiology?
B4. Pigeon.
Organs? Morphologic diagnosis? Special procedure?
021660
B4. Pigeon
Organs? Keywords?
Morphologic diagnosis?
Special procedure?
021660
B4. Pigeon
Duodenum, pancreas, mesentery.
Enlargement of the mesentery, replacement of the adipose tissue.
021660
B4. Pigeon
Subtotal replacement of the pancreas by the inflammatory infiltrate.
021660
B4. Pigeon
Thickening of the duodenal mucosa by the same inflammatory infiltrate as
in the mesentery and pancreas.
021660
B4. Pigeon
Epithelioid macrophages.
Multinucleate giant cells, Langhans type.
Small amount of lymphocytes. GRANULOMATOUS INFILTRATE.
021660
B4. Pigeon
Fite-Farraco stain.
Massive amount of acid-fast bacilli within the cytoplasm of macrophages
and giant cells.
021660
B4. Pigeon
Liver.
Multifocal random granulomas, 100 micrometers to 1 mm in diameter.
021660
B4. Pigeon
Diffuse severe chronic granulomatous enteritis, pancreatitis and
peritonitis with Langhans-type giant cells.
Multifocal marked chronic granulomatous hepatitis.
Note: Mycobacterium avium subsp. silvaticum in pigeons.
