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Exp # 8

This document provides instructions for determining the percentage of acidity in milk through titration. Key steps include taking a 10 ml milk sample and titrating it against a 0.1N NaOH solution until a pink endpoint is reached. By calculating the volume of NaOH used, the percentage of lactic acid in the milk can be found. Reactions involving the neutralization of calcium phosphate, sodium phosphate, and lactic acid by NaOH are provided. The percentage acidity is then calculated based on the volume of NaOH used and known conversions between NaOH volume and grams of lactic acid.

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65 views2 pages

Exp # 8

This document provides instructions for determining the percentage of acidity in milk through titration. Key steps include taking a 10 ml milk sample and titrating it against a 0.1N NaOH solution until a pink endpoint is reached. By calculating the volume of NaOH used, the percentage of lactic acid in the milk can be found. Reactions involving the neutralization of calcium phosphate, sodium phosphate, and lactic acid by NaOH are provided. The percentage acidity is then calculated based on the volume of NaOH used and known conversions between NaOH volume and grams of lactic acid.

Uploaded by

Aasia Farrukh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Chemical Process Industries (lab)

Department of Chemical Engineering Course No. Ch.E-201


Wah Engineering College wahcantt.

EXPERIMENT# 08

Experiment Title: Milk analysis

Objectives: Determine the percentage of acidity in milk by titrating a known volume of milk sample
against a standard solution of sodium hydroxide.

Apparatus required: Titration flask, beaker, burette, pipette, and burner.

Chemicals required: N/10 NaOH solution,1% solution of phenolphthalein, milk.

Procedure:

Take 10 ml of milk sample in the titration flask. Add 40 ml of freshly boiled and cool
water in it. Add 0.5 ml of 1% solution of phenolphthalein in the titration flask. Take N/10 NaOH solution
in the burette. Titrate the milk sample against the N/10 NaOH solution. Note down the initial burette
reading and the final burette reading when the end point , a distinct pink colour is achieved. Calculate the
percentage of acidity in milk sample as below.

Observations and Calculations:

1. Normality of standard solution of NaOH = 0.1N


2. Volume of milk sample taken = 10 ml
3. Indicator = 1% solution of phenolphthalein
4. Initial colour of solution = milky white
5. End point colour of the solution = pink Colour

Reactions:
CaHPO4 + 2NaOH → Ca(OH)2 + Na2HPO4

Na2HPO4 + NaOH → Na3PO4 + H2O

CH3CH(OH)COOH + NaOH → CH3CH(OH)COONa + H2O

[Type text] Page 1


Chemical Process Industries (lab)
Department of Chemical Engineering Course No. Ch.E-201
Wah Engineering College wahcantt.

Sr.No. Initial Burette Reading Final Burette Reading Volume of M/100 EDTA used
(ml) (ml) (ml)

Mean Reading = ________

Volume of N/10 NaOH solution used = ________________________

1 Gm. EQ. of NaOH = 1 Gm. EQ. of lactic acid

1000 ml of 1 N NaOH = 90 g of lactic acid

1.5 ml of 1/10 N NaOH = (90/1000) * (1/10) * 1.5 gm of lactic acid

=________________ gm of lactic acid

%age of acidity in milk W/V = (_________________________/10) * 100

%age of acidity in milk W/WV = [_________________________/(10*density of milk)] * 100

[Type text] Page 2

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