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Position Effects and Genetic Disease

Position effects refer to changes in gene expression that occur when a gene is relocated to a new region of the genome near heterochromatin. This can silence gene expression in some cells through position-effect variegation, where heterochromatin spreads in a differential and heritable manner between daughter cells. While not fully understood, position effects are thought to be mediated by changes to chromatin packaging caused by proteins that modulate transcription factor binding to DNA. Mutations in genes encoding proteins that bind heterochromatin regions, like telomeres, can reduce gene suppression effects.

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62 views1 page

Position Effects and Genetic Disease

Position effects refer to changes in gene expression that occur when a gene is relocated to a new region of the genome near heterochromatin. This can silence gene expression in some cells through position-effect variegation, where heterochromatin spreads in a differential and heritable manner between daughter cells. While not fully understood, position effects are thought to be mediated by changes to chromatin packaging caused by proteins that modulate transcription factor binding to DNA. Mutations in genes encoding proteins that bind heterochromatin regions, like telomeres, can reduce gene suppression effects.

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Sharypro
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Position effects and genetic disease ERIC MILOT, PETER FRASER AND FRANK GROSVELD*

*vanderkamp~chl.fgg.eur.nl THE EP~.~;MI S UNIVEP, SITY, MGC-DEPARTME~ff OF CELL BIOLO(;Y AND GENETICS, PO BOX 1738, 3000 DR
Ro'rIERDAM, THE NETHERI.~.5;DS.

Position effects

The eukaryotic genome can be roughly divided into two cytologically distinguishable states:
euchromatin and heterochromatin L2. The eu- chromatin regions are decondensed in interphase.
They replicate early and contain mostly single-copy se- quences and genes. In contrast, the
heterochromatin regions remain con- densed throughout the cell cycle, usu- ally replicate late
and contain a high proportion of middle-repetitive and highly repetitive sequences . Exactly how
the state o fchromatin modulates gene expression is still largely un- known, although it is very
clear that the chromatin is not simply a passive structural scaffold 4. Position effects (PEs), that
is, a change in the expected level of gene expression, have been associated with integration or
translocation of a gene with other regions of the genome, In some cases, heterochromatic and
euchromatic regions can be juxta- posed, and this can cause a change in expression of the genes
located in the vicinity of the breakpoint s. One particular type of PE, first observed in Drosophila
and yeast, occurs through relocalization of a gene into a heterochromatic environ- ment, which
leads to a shut-down of expression of the gene in some of the cells. This cell-to-cell mosaic ex-
pression could be created by a dif- ferential spreading of the hetero- chromatin. TI, ,is
phenomena, which is donal and heritah!e in daughter cells, is known as podtion-effect varie-
gation (PEV; Ref. 6 ) and has yet ~o be explained fully at the molecular level. This effect can be
altered by the producLs of a number of genes known as enhancers or suppressors of PEV, which
are proteins that are thought to change chromatin pack- aging. The spreading of such factors into
neighbouring areas that contain the relocalized gene is thought to modulate the ability of
transcription factors to bind their target DNA sequences. This is strongly supported by studies on
telomeric silencing in yeast. Gene suppression is reduced by mutations occurring in SIR2, SIR3,
SIR4, NATI, ARD1 and RAP/, which encode factors that bind to telomeric regions 7. Other
spatial effects on gene expression have been observed and correlated to the presence of these
proteins. For example, RAP/is known to be present at high concentration in spots near the
nuclear envelope s and is proposed to be invoh,ed in the repression of genes in these areas of the
nucleus. Suppression by re

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