Biofloc in Aquaculture
High porosity aggregate in water body; made of
microorganism, particle, colloid, and organic matter
Biotic component (interaction of Abiotic
microorganism) component
Plankton, Particle,
Microalgae Bacteria colloid, organic
protozoa matter
(Junda, 2011)
Figure 1. Bioflok yang terapung
( www.aiyu.com, 2011)
Biofloc Production
• Flok adalah agregat yang terbentuk oleh bakteri berfilamen,
plankton, polimer organik, dan sel-sel mikroba yang telah
mati, dan disatukan oleh polimer ekstraseluler.
• Pembentukannya dipengaruhi oleh C/N
• Perannya bagi mikroba adalah untuk meningkatkan transpor
substrat ke dalam sel dalam medium yang substratnya
terbatas.
Produksi Bioflok
Biofloc Production
Natural resources of biofloc : BIOFLOC REACTOR
- Uncertainity floc composition Consorsium
- Unstable floc performance microalgae and
- Uncontroled water quality parameter bacteria to
optimize nutrient
cycle
CONTOH SKEMA PRODUKSI BIOFLOK
Hasil Seleksi Mikroba Pembentuk Bioflok dan Optimasi C/N
• Produce carbohydrate, lipid ,and
Nutrition
protein as nutrition for white
supplement shrimp
Water quality • Utilize ammonia so that support
control zero water discharge system
• Produce bacteriosin against
probiotic-like pathogen
Inoculation Observation Application Treatments Analyse
• C. gracilis • Structure • Optimum • Food • Water
and and biofloc culture conversion quality
nitrifying activity period of ratio
bacteria biofloc • Bacteria
diversity
BIOFLOC GROWTH
0,2000
0,1500
gram
0,1000
0,0500
0,0000
0 5 10 15
Day -
Suantika et al, 2011
Application of Biofloc
Example: application of biofloc in shrimp nursery
Nitrifying Bacteria, microalgae Chaetoceros gracilis
gracilis,, and
Probiotic Halomonas aquamarina
Suantika et al., 2011
Role of Biofloc technology in aquaculture :
Vibrio harveyi
5,00
4,00
log jumlah sel Vibrio
3,00 A (100% pakan)
B (90% pakan : bioflok-probiotik)
C (80% pakan : bioflok-probiotik)
2,00
D (70% pakan : bioflok-probiotik)
E (60% pakan : bioflok-probiotik)
1,00
0,00
0 2 4 6 8 10
minggu ke-
Vibrio harveyi load on nursery
Gede Suantika et al, 2011
White shrimp ( post larvae 13 ) in Bacteria Microalgae
35 days cultured with biofloc:
Combination of microalgae
Thalasiossira sp and bacteria
Achromobacter liquafacreas
Achromobacter sp. Thalassiosira sp.
Cellulose and pectin Diatoms
degradation Phytoplankton
Chitin hydrolysis as prime producer
Reduce accumulation of High salinity tolerance
phosphate
High salinity tolerance
(Suantika et al, 2007)
White shrimp ( post larvae 13 ) in 35 days cultured with :
BIOFLOC : combination of microalgae Thalasiossira sp and
bacteria Achromobacter liquafacreas
FCR SURVIVAL RATE TOTAL
BIOMASS
100 % feed 1,85 44 % 7,88 gram
10% v/v B, 50% feed 1,05 51,33 % 8,57 gram
10% v/v B, 75% feed 1,03 61,33 % 13,50 gram
10% v/v Biofloc - < 20 % 0,22 gram
(Suantika et al, 2007)
A low or zero water exchange production “tool” used
primarily in intensive shrimp / fish production
Utilizing pond microbial community to improve water
quality and maximize feed recycling
Promotion of stable, heterotrophic bacteria dominated
pond environment instead of phytoplankton
dominated that is light sensitive and highly unstable
Bacteria thrive and “floc” and flocs assimilate waste
nutrients
In short – your pond becomes your biofilter!
Thank You
